跳到主要內容

臺灣博碩士論文加值系統

(35.168.110.128) 您好!臺灣時間:2022/08/16 06:12
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:陳冠丞
論文名稱:闊葉大豆(金門一條根)之抗氧化特性及其有效多酚類化合物之分離
論文名稱(外文):The Antioxidative and Bioactive Componentsof Root Extract from Glycine tomentella Hayata
指導教授:孫寶年孫寶年引用關係廖若川
學位類別:碩士
校院名稱:國立海洋大學
系所名稱:食品科學系
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
中文關鍵詞:一條根脂氧合酵素抗氧化多酚類天然物
外文關鍵詞:Glycine tomentella HayataI-Tiao-Gunglipoxygenaseantioxidationpolyphenol
相關次數:
  • 被引用被引用:23
  • 點閱點閱:1730
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:3
二年生金門產一條根(闊葉大豆之主根部)樣品長度為12.0 ~ 14.3 cm。一條根之水、50 %乙醇以及95 %乙醇粗萃取物中,多酚類 (gallic acid equivalent) 含量分別為34.82、103.72以及88.54 mg/g,而三種溶劑中以95 %乙醇之萃取產率為最高(12.0%),其次為水萃取之產率(10.1%),50 %乙醇萃取產率最低(9.4%)。
對血色素催化亞麻油酸(linoleic acid)的抑制能力,以95 %乙醇粗萃取物之IC50最低 (4.63 μg/ml),其次為50 %乙醇粗萃取物 (IC50=9.06 μg/ml),水粗萃取物抑制脂肪酸氧化之能力為三者中最低(IC50=28.16 μg/ml),一條根萃取溶劑之極性較低,其萃取物之抗氧化性較高。
掃除DPPH (α, α-diphenyl-β-picrylhydrazyl)自由基之抗氧化試驗中,發現三種粗萃取物皆具有掃除DPPH自由基之抗氧化特性,其掃除之能力依序為95 %乙醇粗萃取物 > 50 %乙醇粗萃取物 > 水粗萃取物,其結果與抑制脂肪酸自氧化之能力一致。
金門產一條根粗萃取物對於黃豆脂氧合(soybean lipoxygenase, LOX)皆具有抑制作用,產物與發光劑luminol產生之冷光,以95 %乙醇粗萃取物最高,水粗萃取物居末;三者皆可降低15-LOX催化花生四烯酸(eicosatetraenoic acid)形成之15-氫過氧化物(15- hydroperoxy eicosatetraenoic acid, 15-HpETE)之產量,其抑制率以95 %乙醇粗萃取物最高;三種粗萃取物皆具有螯合鐵離子之能力,可能會與LOX活性中心之鐵離子產生螯合,使酵素失活。
三種一條根粗萃取物對於人類血液流變性質之影響,根據冪次方程式σ=K۰γn ,其流體行為指標(fluid behavior index, n)皆為0.71,皆較未添加一條根萃取物之血液(n=0.65)更接近牛頓型流體(n=1);而添加三種一條根萃取物黏稠指標(fluid consistency, K)皆為0.08,較控制組(K=0.13)低,代表一條根萃取物具有降低血液黏稠度之功能,並增加紅血球細胞膜之完整性。
一條根95%乙醇粗萃取物利用polyamide管柱及流動相水、10 %乙醇、30 %乙醇、50 %乙醇、70 %乙醇、95 %乙醇、氯仿-甲醇(1:1)、及甲醇分離,發現
50 %乙醇分離物在A254nm之純度為88.6 %,並經呈色試驗推測為黃酮類,在UV及可見光譜之吸光波峰類似epicatechin;此分離物具有抑制亞麻油酸自氧化、掃除DPPH之能力、及抑制LOX作用亞麻油酸產物與luminol反應之冷光之抗氧化特性,但不具螯合
I-Tiao-Gung, root of Glycine tomentella Hayata, used in this study was about 12.0 ~ 14.3 cm in length. The polyphenol content expressed as gallic acid equivalent extracted from I-Tiao-Gung using water, 50 % ethanol and 95 % ethanol as solvent were 34.82, 103.72 and 88.54 mg/g respectively. Extraction with 95 % ethanol gave the highest yield (12.0 %).
Inhibition of hemoglobin-induced linoleic acid (C18:2) oxidation by root extracts of I-Tiao-Gung was indicated by the IC50 being 4.63, 9.06, 28.16 μg/ml, for the 95 % ethanol, 50 % ethanol, water extract, decreased with increased polarity of the extraction solvent.
All three crude extracts had free radical-scavenging activity determined with DPPH. The capacity was similar with inhibition of lipid autoxidation in an order of 95 % ethanol extract > 50 % ethanol > water.
Inhibition on the soybean lipoxygenase catalyzed arachidonate oxidation was the highest with 95 % ethanol extract, and followed the same order in reducing 15-hydroperoxy eicosatetraenoic acid (15-HpETE) formation shown by HPLC, and chemiluminescence intensity using luminol. All three crude extracts chelated iron ion, while the lipoxygenase active site has Fe3+.
Blood thinning effects were observed in vitro by mixing the extract of I-Tiao-Gung with human red blood cells. The rheological data fitted the power law model, σ = K γ n, where σ =shear stress, γ = shear rate, K = fluid consistency, n =flow behavior index, with a correlation coefficient of R2 = 0.99 at a constant hematocrit of 44 %. The addition of extract resulted in a reduced K from 0.13 to 0.08, and an increased n from 0.65 to 0.71 indicative of a change in flow behavior closer to Newtonian type and a thinning consistency.
Separation of the crude 95 % ethanol extract of I-Tiao-Gung using polyamide column eluted by water, 10 % ethanol 30 % ethanol, 50 % ethanol, 70 % ethanol, 95 % ethanol, methanol-chloroform (1:1), and methanol as mobil phase, obtained one fraction eluted by 50 % ethanol showing a purity of 88.6 % as detected with A254nm. The fraction had color reactions and UV-Vis spectrum similar to those of epicatechin. It inhibited linolate autoxidation, and lipoxygenase activity, as well as DPPH free radical scavenging, but did not chelate iron.
中文摘要 …………………………………………………… I
英文摘要 ………………………………………………………… III
壹、前言 ………………………………………………………… 1
貳、文獻整理 ……………………………………………… 4
一、金門產一條根之藥用植物學考察及毒性 ………………… 4
二、多酚類化合物之分佈及定性 ……………………………… 6
三、抗氧化劑對於紅血球與血液黏度之影響 ………………… 7
四、脂氧合與血液黏度之關係 ……………………………… 9
五、脂氧合與心血管疾病的關係 …………………………… 10
六、5-脂氧合在動物體之生理功能 ………………………… 12
七、發炎及過敏反應之抑制 …………………………………… 14
八、12-lipoxygenase及生成物hepoxilin之生理功能………… 17
九、12-lipoxygenase之抑制作用 ……………………………… 19
參、材料與方法 ………………………………………………… 21
一、材料 ………………………………………………………… 21
二、分析方法 ……………………………………………… 21
1. 一條根萃取物之製備………………………………………… 21
2. 總多酚類之定量……………………………………………… 22
3. 對血色素催化亞麻油酸自氧化之抑制作用………………… 22
4. 脂氧合總活性測定………………………………………… 23
5. 逆向高效液相層析法測定脂氧合異構活性…………… 24
6. 血液黏度測定………………………………………………… 25
7. 血液滲透壓之測定…………………………………………… 25
8. 掃除自由基試驗……………………………………………… 25
9. 螯合鐵能力之測定…………………………………………… 26
10. 一條根粗萃取物之初步分離………………………………… 27
11. 酚類化合物之HPLC分析……………………………………… 28
12. 分離物之呈色反應………………………………………… 28
肆、結果與討論 ……………………………………………… 29
一、一條根萃取條件 …………………………………………… 29
1. 溶劑之選擇…………………………………………………… 29
2. 萃取次數及時間之選擇……………………………………… 30
二、一條根粗萃取物之功能特性 ……………………………… 30
1. 產率及多酚類之含量………………………………………… 30
2. 一條根粗萃取物對於脂肪酸自氧化作用之影響…………… 33
(1) 對亞麻油酸自氧化之影響 ……………………………………33
(2) 掃除DPPH自由基之能力 …………………………………… 35
3. 對脂氧合催化不飽和脂肪酸氧化之影響………………… 37
(1) 脂氧合總活性抑制………………………………………… 38
(2) 脂氧合異構之抑制 …………………………………… 40
4. 一條根粗萃取物對人血液特性之影響.……………………… 42
三、一條根萃取物多酚類之組成與抗氧化性…………………… 45
1. HPLC分離條件之確定………………………………………… 45
2.一條根分離物之多酚類含量及組成…………………………… 48
(1)水分離物 ……………………………………………………… 48
(2) 10 %乙醇分離物……………………………………………… 52
(3) 30 %乙醇分離物……………………………………………… 54
(4) 50 %乙醇分離物……………………………………………… 56
(5) 70 %乙醇分離物……………………………………………… 59
(6) 95 %乙醇分離物、氯仿-甲醇分離物、及甲醇分離物…… 60
3. 95%乙醇分離物經polyamide管柱層析分離物之抗氧化性… 61
(1) 對黃豆脂氧合作用亞麻油酸之影響 …………………… 61
(2) 對血色素催化脂質自氧化之影響…………………………… 64
伍、結論 ………………………………………………………… 66
陸、參考文獻 …………………………………………………… 68
林文川、柯裕仁與吳岳文。2000。闊葉大豆(金門產一條根)水粗萃取物對大鼠28天餵食毒性作用。中國醫藥學院雜誌,11:205-215。
林幸榮。1998。抗氧化物在心臟血管疾病預防與治療的最新發展。臨床醫學,42: 157-162。
肖崇厚。1997。中藥化學。新學書店。上海。中國。
吳禮字、吳岳文、施純青、柯裕仁與林文川。2001。台灣金線連和闊葉大豆(金門產一條根)之初步基因毒性試驗。中國醫藥學院雜誌,12:173-178。
柯裕仁、謝文全、邱年永與陳忠川。1999。市售一條根類藥材之藥用植物學考察。中國醫藥學院雜誌,8:33-46。
許秀華。1991。飼料中添加維生素E對養殖烏魚血液黏度及紅血球脂肪酸組成的影響。國立台灣海洋大學水產食品科學研究所碩士論文。
陳存仁,1979。中國藥學大辭典。中正書局出版,台一版。
陳明豐。1997。血管升壓素轉換酵素抑制劑對血管保護-動脈硬化之預防角色。內科學誌,8:103-108.
陳素鳳。1993。攝食維生素E及魚油對於養殖烏魚血液脂氧合特性及血液黏度的影響。國立台灣海洋大學水產食品科學研究所碩士論文。
劉玉茹。2000。鎘污染及綠茶液對魚血液、鰓脂氧合及白三烯素形成之影響。國立台灣海洋大學食品科學研究所碩士論文。
Antόn, R., L. Puig, T. Esgleyes, J.M. Moragas and L. Vila. 1998. Occurrence of hepoxilins and trioxilins in psoriatic lesions. J. Invest. Dermatol., 110:303-310.
Ardlie, N. G., M.L. Selley and L.A. Simms. 1989. Platelet activation by oxidatively modified low density lipoproteins. Atherosclerosis, 76:117-124.
Aruoma, O.I. 1998. Free radicals, oxidative stress, and antioxidants in human health and dieases. JAOCS, 75:199-212.
Berliner, J.A. and A.D. Watson. 1995. The role of oxidized lipid in cardiovascular disease. pp. 433-449. In Valentine, J.S., C.S. Foote, A. Greenberg and J.F. Liebman.(Eds.) Active Oxygen In Biochemistry. Chapman & Hall. New York.
Brand-Williams, W., M.E. Cuvelier and C. Berset. 1995. Use of a free radical method to evaluate antioxidant activity. Technology, 28:25-30.
Cadenas, S., C. Rojas, R. Pérez-Campo, M. López-Torres and G. Barja. 1995. Vitamin E protects guinea pig liver from lipid peroxidation without depressing levels of antioxidants. Int. J. Biochem.Cell Biol., 27:1175-1181.
Cai, X., R.T. Scannell, D. Yaeger, Md.S. Hussoin, D.B. Killian, C. Qian, J. Eckman, S.B. Hwang, L. Libertine-Garahan, C.G. Yeh, S.H. Ip and T.Y. Shen. 1998. (±)-trans-2-[3-Methoxy-4-(4-chlorophenylthioethoxy)-5-(N- methyl-N-hydroxyureidyl)methylphenyl]-5-(3,4,5-trimethoxyphenyl) tetrahydrofuran (CMI-392), a potent dual 5-lipoxygenase inhibitor and platelet-activating factor receptor antagonist. J. Med. Chem., 41:1970-1979.
Carbonneau, M.A., C.L. Leger, B. Descomps, F. Michel and L. Monnier. 1998. Improvement in the antioxidant status of plasma and low-density lipoprotein in subjects receiving a red wine phenolics mixture. JAOCS, 75:235-240.
Chalas, J., C. Claise, M. Edeas, C. Messaoudi, L. Vergnes, A. Abella and A. Lindenbaum. 2001. Effect of ethyl esterification of phenolic acids on low-density lipoprotein oxidation. Biomed. Pharmacother., 55:54-60.
Chen, H.Y. and G.C. Yen. 1998. Free radicals, antioxidant defenses and humans health. Nutri. Sci. J., 23:105-121.
Chi, Y.S., H.G. Jong, K.H. Son, H.W. Chang, S.S. Kang and H.P. Kim. 2001. Effect of naturally occurring prenylated flavonoids on enzymes metabolizing arachidonic acid: cyclooxygenases and lipoxygenases. Biochem. Pharmacol., 62:1185-1191.
Chien, S., S. Usami, R.J. Dellenback and M.I. Gregersen. 1967. Blood viscosity: influence of erythrocyte deformation. Science, 157:827-829.
Chien, S., S. Usami, R.J. Dellenback and M.I. Gregersen. 1971. Comparative hemorheology- hematological implication of species differences in blood viscosity. Biorheology, 8:35-57.
Chiu, D. and B. Lubin. 1989. Oxidative hemoglobin denaturation and RBC destruction the effect of heme on red cell membranes. Seminar in Hematology. 26:128-135.
Chuang, W.L., B. Sun Pan and J.S. Tsai. 2000. Inhibition of lipoxygenase and blood thinning effects of mackerel protein hydrolysate. J. Food Biochem., 24:333-343.
Chung, T.W., T.Z. Chen, J.J.H. Yu, S.Y. Lin and S.C. Chen. 1995. Effect of α-tocopherol nicotinate on hemorheology and retinal capillary blood flow in female NIDDM with retinopathy. Clin. Hemorheol., 15:775-782.
Coffey, M.J., C.S. Wheeler, K.B. Gross, W.L. Eschenbacher, P.H.S. Sporn and M. Peters-Golden. 1996. Increased 5-lipoxygenase metabolism in the lung of human subjects exposed to ozone. Toxicology, 114:187-197.
Fletcher, G.L. and R.T. Haedrich. 1987. Rheological properties of rain bow trout blood. Can. J. Zool., 65:879-883.
Frémont, L. 2000. Biological effects of resveratrol. Life Sciences, 66:663-673.
Frenette, R., J.H. Hutchinson, S. Léger, M.Thérien, C. Brideau, C.C. Chan, S. Charleson, D. Ethier, J. Guay, T.R. Jones, M. McAuliffe, H. Piechuta, D. Riendeau, P. Tagari and Y. Girard. 1999. Substituted indoles as potent and orally active 5-lipoxygenase activating protein (FLAP) inhibitors. Bioorg. Med. Chem. Letters, 9:2391-2396.
Fujii, M., N. Matsumura, K. Mito, T. Shimizu, M. Kuwahara, S. Sugano and H. Karaki. 1993. Antihypertensive effects of peptides in autolysate of bonito bowels on spontaneously hypertensive rats. Biosci. Biotech. Biochem., 57:2186-2188.
Fujimoto, Y., S. Sakuma, M. Tsunomori, T. Sumiya and T. Fujita. 1999. Sphingomyelin inhibits platelet 12-lipoxygenase activity. Eur. J. Pharmacol., 378:233-236.
German, J.B. and R. Berger. 1990. Formation of 8,15-dihydroxy eicosatetraenoic acid via 15- and 12-lipoxygenases in fish gill. Lipids, 25:849-853.
Gordon, D.O. and M.D. Lowe. 1988. Clinical Blood Rheology, Volume I. CRC Press, Inc. Boca Raton, Florida, pp.1-10.
Grinberg, L.N., H. Newmark, N. Kitrossky, E. Rahamim, M. Chevion and E.A. Rachmilewitz. 1997. Protective effects of tea polyphenols against oxidative damage to red blood cells. Biochem. Pharmacol., 54:973-978.
Halder, J. and A.N. Bhaduri. 1998. Protective role of bleak tea against oxidative damage of human red blood cells. Biochem. Biophys. Res. Commun., 244:903-907.
Harris, R.R., G.W. Carter, R.L. Bell, J. L. Moore and D. W. Brooks. 1995. Clinical activity of leukotriene inhibitors. Int. J. Immunopharmac., 17:147-156.
Henderson, W.R. 1994. The role of leukotrienes in inflammation. Annals of Internal Meddicine, 21:684-697.
Holman, R.T., P.O. Egwin and W.W. Christie. 1969. Substrate specificity of soybean lipoxidase. J. Biol. Chem., 244:1149-1151.
Hsieh, R.J., J.B. German and J.E. Kinsella. 1988. Lipoxygenase in fish tissue: Some properties of the 12-lipoxygenase from trout gill. J. Agric. Food Chem., 36:680-685.
Hsien, M.T., Y.L. Chang and C.F. Chen. 1991. Effects of Tao-Hung-Tang on haemorheological disturbance induced by thrombus formation in rats. J. Clim. Med., 2:76-85.
Jain, S.K., R. Mcvie, J. Duett and J.J. Herst. 1989. Erythrocyte membrane lipid peroxidation and glycosylated hemoglobin in diabetes. Diabetes, 38:1539-1543.
Kelman, D.J., J.A. Drgray and R.P. Mason. 1994. Reaction of myoglobin with hydrogen peroxide forms a peroxyl radical which oxidizes substrates. J. Biol. Chem., 269:7458-7463.
Kinsella, J.E. 1987. Effect of polyunsaturated fatty acid on factors related to cardiovascular disease. Am. J. Cardiol., 30:23-32.
Kishimoto, K., M. Nakamura, H. Suzuki, T. Yoshimoto, S. Yamamoto, T. Takao, Y. Shimonishi and T. Tanabe. 1996. Suicide inactivation of porcine leukocyte 12-lipoxygenase associated with its incorporation of 15-lipoxygenase-5,8,11,13-eicosatetraenoic acid derivative. Biochimica et Biophysica Acta., 1300:56-62.
Koketsu, M. 1997. Antioxdative activity of tea polyphenol. pp.37-50. In Yamamoto, T., L.R. Juneja, D.C. Chu and M. Kim. (Eds.) Chemistry and Applications of Green Tea. CRC Press LLC. New York.
Kühn, H., J. Belkner, S. Zaiss, T. Fährenlemper and S. Wohlfeil. 1994. Involvement of 15-lipoxygenase in early stages of atherogenesis. J. Exp. Med., 179:1903-1911.
Kühn, H. and B.J. Thiele. 1999. The diversity of the lipoxygenase family. FEBS Lett., 449:7-11.
Kuo, J.M., D.B. Yeh and B.S. Pan. 1999. Rapid photometric assay evaluating antioxidative activity in edible plant material. J. Agric. Food Chem., 47:3206-3209.
Leira, E., A. Botana and R. Cela. 1996. Resolution of complex mixtures of non-flavonoid polyphenols by column-switching high- performance liquid chromatography using octadecylsilica and graphitized carbon columns. J. Chromatogr. A, 724:67-78.
Lu, Y. and L.Y. Foo. 2000. Antioxidant and radical scavenging activities of polyphenol from apple pomace. Food Chem., 68:8185.
Maiani, G, M. Serafini, M. Salucci, E. Azzini and A. Ferro-Luzzi. 1997. Application of a new high-performance liquid chromatographic method for measuring selected polyphenols in human plasma. J. Chromatogr. B, 692:311-317.
Malamas, M.S., R.P. Carlson, D. Grimes, R. Howell, K. Claser, I. Gunawan, J.A. Nelson, M. Kanzelberger, U. Shah and D.A. Hartman. 1996. Azole phenoxy hydroyureas as selective and orally active inhibitors of 5-lipoxygenase. J. Med. Chem., 39:237-245.
Matsumura, N., M. Fujii, Y. Takeda and T. Shimizu. 1993. Isolation and characterization of angiotensin I-coverting enzyme inhibitory peptides derived from bonito bowels. Biosci. Biotechnol. Biochem., 57:1743-1744.
Merken, H.M. and G.R. Beecher. 2000. Measurement of food flavonoids by high-performance liquid chromatography: A review. J. Agric. Food Chem. (Review), 48:577-599.
Metzger, K., G. Angres, H. Maier and W.D. Lehmann. 1995. Lipoxygenase products in human saliva: patients with oral cancer compared to controls. Free Radical Biology & Medicine, 18:195-194.
Mita, H., Y. Yui, N.Taniguchi, H. Yasueda and T. Shida. 1985. Increased activity of 5-lipoxygenase in polymorphonuclear leukocytes from asthmatic patients. Life Science, 37:907-914.
Műller, K. and I. Gawlik. 1995. Novel 10-substituted anthrones as inhibitors of epidermal 12-lipoxygenase and lipid peroxidation in membranes. Biochem. pharmacol., 50:2077-2083.
Műller, K. and I. Gawlik. 1996. Inactivation of mouse epidermal 12-lipoxygenase by anthralin- Implications for the role of oxygen redicals. Biochem. pharmacol., 51:1173-1179.
Natarajan, R., H. Pei, J.L. Gu, J.M. Sarma and J. Nadler. 1999. Evidence for 12-lipoxygenase induction in the vessel wall following balloon injury. Cardiovasc. Res., 41:489-499.
Pace-Asciak, C.R., D. Reynaud and P. Demin. 1995. Mechanistic aspects of hepoxilin biosynthesis. J. Lipid Mediators Cell Signalling, 12:307-311.
Pan, B.S., H.H. Hsu, S. Chen and H.M. Chen. 1999. Effects of α-tocopherol on lipoxygenase-catalyzed oxidation of highly unsaturated fatty acid. pp. 76-85. In Whitaker, J.R., N.F. Haard, C.F. Shoemaker and R.P. Singh (Eds.). Food for Health in the Pacific Rim. Food & nutrition Press, Inc. Trumbull, Ct. USA.
Parthasarathy, S. and S.M. Rankin. 1992. Role of oxidized low density lipoprotein in atherogensis. Prog. Lipid Res., 31:127-143.
Pietta, P.G. 2000. Flavonoids as antioxidants. J. Nat. Prod., 63:1035-1042.
Puerta, R., V.R. Gutierrez and J.R.S. Hoult. 1999. Inhibition of leukocyte 5-lipoxygenase by phenolics from virgin olive oil. Biochemical Pharmacology, 57:445-449.
Rankin, S.M., S. Parthasarathy and D. Stcincerg. 1991. Evidence for a dominant role of lipoxygenase(s) in the oxidation of LDL by mouse peritoneal. J. Lipid Res., 32:449-456.
Rao, S.I., A. Wilks, M. Hamberg and P.R. Ortiz de Montellano. 1994. The lipoxygenase activity of myoglobin. Oxidation of linoleic acid by the ferryl oxygen rather than protein radical. J. Biol. Chem., 269:7210-7216.
Revilla, E. and J.M. Ryan. 2000. Analysis of several phenolic compounds with potential antioxidant properties in grape extracts and wines by high-performance liquid chromatography-photodiode array detection without sample preparation. J. Chromatogr. A, 881:461-469.
Ross, R. 1993. The pathogenesis of atherosclerosis: A perspective for 1990s. Nature, 362:801.809.
Samuelsson, B. 1991. Arachidonic acid metabolism: role in inflammation. Z. Pheumatol. 50:3-6.
Samuelsson, B. and C.D. Funk. 1989. Enzymes involved in the biosynthesis of leukotriene A4. J. Biol. Chem., 264:19469-19472.
Samuelsson, B., S.K. Dahlen, J.A. Lindgren, C.A. Rouzer and C.N. Serhan. 1987. Leukotrienes and lipoxins: Structure, biosynthesis and biological effects. Science, 237:1171-1175.
Sánchez-Moreno, C., B.Sc., A. Jiménez-Escrig, B. Sc., F. Saura-Calixto, Ph. D. Prof. 2000. Study of low-density lipoprotein oxidizability indexes to measure the antioxidant activity of dietary polyphenols. Nutrition Research, 20:941-953.
Scheidegger, K.J., S. Bultler and J.L. Witztum. 1997. Angiotensin II increases macrophage-mediated modification of low density lipoprotein-dependent pathway. J. Bio. Chem., 272:21609-21615.
Schewe, T. and H. Kűhn. 1991. Do15-lipoxygenases have a common biological role? Elsevier Science Publishers, 16:369-373.
Sendobry, S.M., J.A. Cornicelli, K. Welch, T. Bocan, B. Tait, B.K. Trivedi, N. Colbry, R.D. Dyer, J. Feinmark and A. Daugherty. 1997. Attenuation of diet-induced arteriosclerosis in rabbits with a highly selective 15-lipoxygenase in inhibitor lacking significant antioxidant properties. Brit. J. Pharmacol., 120:1199-1206.
Seppo, Y.H. 1991.Macrophages and oxidized low density lipoxygenase in the pathogenesis of atherosclerosis. Annals of Med., 23:561-567.
Serhan, C.N. and M. Romano. 1995. Lipoxin biosynthesis and action: role of the human platelet LX-synthase. J. Lipid Mediators Cell Signalling, 12:293-306.
Shahrzad S. and I. Bitsch. 1998. Determination of gallic acid and its metabolites in human plasma and urine by high-performance liquid chromatography. J. Chromatogr. B, 705:87-95.
Sigal, E., C.W. Laughton and M.A. Mulkins. 1994. Oxidation, lipoxygenase, and atherogenesis. Annals New York Academy of Sciences, 714:211-224.
Singleton, V.L. and J.A. Russi, J.R. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic., 16:382-388.
Snyder, L.M., N.J. Fortier, J. Trainor, J. Jacobs, L. Leb, B. Lubin, D. Chiu, S. Shohet and N. Mohandas. 1985. Effect of hydrogen peroxide exposure on normal human erythrocyte deformability, morphology, surface characteristics, and spectrin-hemoglobin cross-linking. J. Clin. Invest., 76:1971-1977.
Sun, D. and C.D. Funk. 1996. Disruption of 12/15-lipoxygenase expression in peritoneal macrophages. Biol. Chem., 271:24055-24062.
Sutherland, M., T. Schewe and S. Nigam. 2000. Biological actions of the free acid of hepoxilin A3 on human neutrophils. Biochem. Pharmacol., 59:435-440.
Tedesco, I., M. Russo, Paola, G. Lacomino, G.L. Russo, A. Carraturo, C. Faruolo, L. Moio and R. Palumbo. 2000. Antioxidant effect of red wine polyphenols on red blood cells. J. Nutr. Biochem, 11:114-119.
Vázquez, G., G. Antorrena, J. González and S. Freire. 1995. Studies on the composition of pinus pinaster foliage. Bioresource Technology, 51:83-87.
Virmani, J., E.N. Johnson, A.J.P. Klein-Szanto and C.D. Funk. 2001. Role of ‘platelet-type’ 12-lipoxygenase in skin carcinogenesis. Cancer Lett., 162:161-165.
Werz, O., D. Szellas, M. Henseler and D. Steinhilber. 1998. Nonredox 5-lipoxygenase inhibitors require glutathione peroxidase for efficient inhibition of 5-lipoxygenase activity. Mol. Pharmacol., 54:445-451.
Wu, C.H., W.L. Dai, H.Y. Tsai, M.T. Hsieh and W.C. Chang. 1999. The antiatherogenic effects of Magnolia Officanalis and Alismatis Rhizoma. Clin. Med. J., 8:17-23.
Yokoyama, K., H. Chiba and M. Yoshikawa. 1992. Peptide inhibitors for angiotensin I-converting enzyme from thermolysin digest of dried bonito. Biosci. Biotech Biochem., 56:1541-1545.
Yoshimoto, T. and S. Yamamoto. 1995. Arachidonate 12-lipoxygenase. J. Lipid Mediators Cell Signalling, 12:195-212.
Young, R.N. 1999. Inhibitors of 5-lipoxygenase: a therapeutic potential yet to be fully realized? Eur. J. Med. Chem., 34:671-685.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top