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研究生:游秋虹
研究生(外文):CHIU-HUNG YU
論文名稱:斑馬魚SIM基因對頭骨發育的影響
論文名稱(外文):ZEBRAFISH SIM GENE AND HEAD SKELETON DEVELOPMENT
指導教授:胡清華胡清華引用關係
指導教授(外文):CHIN-HWA HU
學位類別:碩士
校院名稱:國立海洋大學
系所名稱:水產生物技術研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:87
中文關鍵詞:斑馬魚頭骨發育
外文關鍵詞:SIM
相關次數:
  • 被引用被引用:2
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由近來的研究中發現,一群bHLH-PAS蛋白在脊椎動物的胚胎發育過程中扮演著重要的角色。其中sim是bHLH-PAS蛋白群之一,並由研究証實與中樞神經系統的發育有密切關係。人類的sim2 gene位於人類第21條染色體上的唐氏症候群染色體區(Down syndrome critical region, DCR),經研究証實過量表現hsim2所造成神經解剖學(neuroanatomic)及神經化學(neurochemical)異常發育的情況,與唐氏症病患的表現型相似。
先前,本實驗室成功的自斑馬魚(zebrafish)胚胎中選殖出一段sim基因,利用RNA全覆式原位雜交法觀察,結果顯示,SIM 會表現於間腦、頷弧和舌弧。為了進一步了解SIM 在斑馬魚胚胎發育過程中所扮演的角色,因此構築了三種不同的質體,在斑馬魚卵受精後的1-2個細胞時期,進行顯微注射,結果顯示,在注射pCMV-SIM2(150pg)及pCMV-AB(150pg)的斑馬魚胚胎,其顱底(neurocranium)及咽骨(mandibular arch , hyoid arch)的發育有異常的情形,接著更進一步利用專一性標示基因shh、msxB、dlx3、gsc進行RNA全覆式原位雜交法,發現其於斑馬魚胚胎中皆有異常的表現,因此本實驗証實SIM2基因在斑馬魚頭骨發育中扮演著重要的角色。

  Recently , a novel bHLH-PAS protein family were revealed to have important roles in vertebrate development. Single-minded(sim) is a member of bHLH-PAS family that acts important role in central nervous system development. The human sim homolog , hsim2, was identified in the Down syndrome critical region of chromosome 21. It was proposed that the trisomy of the hsim2 gene induces the neuroanatomic and neurochemical abnormalities that often occur in DS.
Recently we have isolated a sim2 homolog gene from a zebrafish cDNA library (AF363019) .The expression pattern of zebrafish sim in the developing embryo was revealed by whole mount in situ hybridization. It shows that sim mRNA was detectable at diencephalons, mandibular arch and hyoid arch. To investigate the functions of sim in zebrafish development, we have microinjected a variety forms of sim DNA into fertilized embryo at 1-2 cell stages. Ectopic expression of sim2 gene or its C-terminal truncation form induced abnormal neurocranium and pharyngeal skeleton development and the expression pattern of midline regulator , shh , and downstream target of ET-1(endothelin-1) , including mexB, dlx3,gsc, were all disturbed by the ectopic sim2 expression , it suggests that sim2 gene plays important role in head skeleton development .

目錄
中文摘要……………………………………………………1
英文摘要…………………………………………………….2
壹、 緒論……………………………………………………4
貳、 實驗材料與方法………………………………………14
參、 結果……………………………………………………33
肆、 討論……………………………………………………39
伍、 參考文獻………………………………………………43
陸、 圖表……………………………………………………52

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