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研究生:蔣捷名
論文名稱:Serratiamarcescens幾丁質酶之蛋白質工程
論文名稱(外文):Protein Engineering of the Chitinases from Serratia marcescens
指導教授:林富邦
學位類別:碩士
校院名稱:國立海洋大學
系所名稱:水產生物技術研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:130
中文關鍵詞:幾丁質幾丁聚醣幾丁寡糖幾丁質酶黏質沙雷氏菌基因工程蛋白質工程
外文關鍵詞:chitinchitosanchito-oligochitinaseSerratia marcescensDNA shufflingError prone PCRMutagenic PCR
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  • 被引用被引用:6
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以聚合酶鏈反應 ( Polymerase Chain Reaction )夾出Serratia marcescens CCRC 15362之幾丁質酶 (Chi22)基因全長。S. marcescens幾丁質酶(Chi22)基因全長675個鹼基對,可轉譯出225個胺基酸的蛋白質。於E. coli BL21(DE3)pLysS所表現的重組蛋白質為不溶性的內涵體 (inclusion bodies ),且難以renaturation恢復活性。
參考已發表文獻中S. marcescens Chi(L38484)與Chi(AB015998)幾丁質酶的胺基酸序列,針對S. marcescens CCRC 15362幾丁質酶基因中可能導致蛋白質為不溶性的序列設計了定點突變的引子,並以PCR定點突變此基因,選殖並表現於E. coli BL21(DE3)pLysS,此重組幾丁質酶SMChi22mut由原先不溶性的內涵體變為可溶的蛋白質,但仍無活性。嘗試以DNA shuffling及Error prone PCR等方式進行突變後,亦篩選不到具有活性的菌株,再次比對文獻與突變後的此基因序列,針對可能導致蛋白質無活性的序列設計了定點突變的引子,並以PCR定點突變此基因,但仍無幾丁質酶活性。
The gene encoding the chitinases (chi22) from Serratia marcescens was amplified from its chromosomal DNA by PCR techniques. The full-length chitinase (chi22) gene of S. marcescens is consisted of 675 base pairs and encode 225 amino acid residues. The recombinant chitinase was expressed in E. coli BL21(DE3)pLysS as insoluble inclusion bodies and were difficult to be renatured.
A site-directed mutagenesis of S. marcescens chitinase gene was performed by PCR based on the amino acid sequence homology comparision of the chitinases among S. marcescens Chi(L38484) and S. marcescens Chi(AB015998). The mutant gene SMchi22mut was cloned and expressed as a soluble protein from the bacterial expression host E. coli BL21(DE3)pLysS. Unfortunately, it had no chitinase activity. DNA shuffling and Error prone PCR were both used to mutate SMchi22mut gene. Any mutant with significant chitinase activity were not found.
The second site-directed mutation of SMchi22mut gene was performed by PCR based on the amino acid sequence homology comparision. Again, it failed to have any clones with chitinase activity.
目錄
Abstract …………………………………………………………………i
中文摘要…………………………………………………………………ii
第一章 文獻整理
第一節 黏質沙雷氏菌 ( Serratia marcescens )……………………1
第二節 幾丁質水解酵素…………………………………………………6
第三節 幾丁質與幾丁聚醣………………………………………………17
第四節 基因的人工洗牌 ( DNA Shuffling )…………………………29
第五節 錯誤傾向聚合酶鏈反應 ( Error prone PCR )………………34
第二章 實驗材料與方法
第一節 實驗材料及實驗藥品……………………………………………37
第二節 Serratia marcescens幾丁質酶基因之選殖……………………43
第三節 Serratia marcescens幾丁質酶重組蛋白質之表現、純化……50
第四節Serratia marcescens幾丁質酶基因的人工洗牌…………………57
第五節 Serratia marcescens幾丁質酶基因的定點突變及其表現……60
第六節 Serratia marcescens幾丁質酶基因的錯誤傾向聚合酶鏈反應(Error prone PCR)………………………………………………………63
第三章 實驗結果
第一節 Serratia marcescens幾丁質酶基因之選殖與表現……………66
第二節 Serratia marcescens幾丁質酶基因第一次定點突變及其選殖與表現……………………………………………………………………………78
第三節 Serratia marcescens幾丁質酶基因的人工洗牌及其選殖與表現……………………………………………………………………………89
第四節 Serratia marcescens幾丁質酶基因的錯誤傾向聚合酶鏈反應(Error prone PCR)及其選殖與表現……………………………………95
第五節 Serratia marcescens幾丁質酶基因第二次定點突變及其選殖與表現……………………………………………………………………………98
第四章 討論與結論
第一節 討論………………………………………………………………109
第二節 結論………………………………………………………………113
第五章 參考文獻…………………………………………………………114
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