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研究生:林四美
論文名稱:吳郭魚稚魚錳之最適需求量探討
論文名稱(外文):Studies on the manganese requirements of juvenile hybrid tilapia, Oreochromis niloticus x O. aureus
指導教授:蕭錫延蕭錫延引用關係
學位類別:碩士
校院名稱:國立海洋大學
系所名稱:食品科學系碩士在職專班
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:57
中文關鍵詞:吳郭魚
外文關鍵詞:manganese requirementjuvenile hybrid tilapia
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摘要
本研究主要探討吳郭魚稚魚對錳的最適需求量。基礎飼料中分別添加錳(MnSO4˙H2O) 0,3,6,10,15,20,30及60 mg Mn/kg diet等共八組實驗飼料,每組飼料三重複分別餵予平均初重0.64  0.02 g吳郭魚稚魚八週。結果顯示吳郭魚之增重百分率、飼料效率、蛋白質利用率、魚體之存活率、肝體比及魚體體組成在各組之間並無差異(P  0.05)。吳郭魚餵食30及60 mg Mn/kg diet之魚體肝臟中錳的含量明顯高於餵食0、3 mg Mn/kg diet組(P<0.05)。骨骼中錳的含量以飼食30及60 mg Mn/kg diet明顯高於餵食0,3,6,10,15,20 mg Mn/kg diet組(P<0.05)。全魚體中錳的含量以餵食30 mg Mn/kg diet組明顯高於餵食0,3,6,10,15,20 mg Mn/kg diet組(P<0.05)。餵食3及6 mg Mn/kg diet組之肝臟MnSOD酵素活性明顯高於未添加組(P<0.05)。魚體中錳型過氧化物歧化酶(MnSOD)活性以Broken-line迴歸分析及魚體中錳含量的平衡值以直線線性迴歸求得吳郭魚錳之最適需求量約為7 mg Mn/kg diet。

Abstract
The study was conducted to determine the optimum dietary manganese requirement of juvenile hybrid tilapia (Oreochromis niloticus × O. aureus). Purified diets with 8 graded levels (0, 3, 6, 10, 15, 20, 30 and 60 mg/kg diet) of supplemented MnSO4˙H2O were fed to tilapia (mean body weight 0.64  0.02 g) for 8 weeks. Each diet was fed to triplicate groups of fish. Results indicated that no differences (P > 0.05) in weight gain (WG), feed efficiency (FE), protein efficiency ratio (PER), and survival were observed among the dietary treatments. Fish fed diets supplemented with 30 and 60 mg Mn/kg diet had higher hepatic manganese concentration than fish fed 0 and 3 mg Mn/kg diets. Fish fed diets supplemented with 30 and 60 mg Mn/kg diet had higher bone manganese concentration than fish fed 0, 3, 6, 10, 15 and 20 mg Mn/kg diets. Fish fed diet supplemented with 30 mg Mn/kg diet had higher whole body manganese concentration than fish fed 0, 3, 6, 10, 15 and 20 mg Mn/kg diets. Fish fed diets supplemented with 3, 6 mg Mn/kg diet had higher hepatic manganese superoxide dismutase (MnSOD) activity than fish fed the unsupplemented control diet. Analysis by linear regression of whole body manganese retention and by broken-line regression of the MnSOD activity of fish indicated that the optimum dietary Mn concentration in juvenile tilapia is approximately 7 mg/kg diet.

目錄
摘要(中文) -----------------------------------------------Ⅰ
摘要(英文) -----------------------------------------------Ⅱ
文獻整理---------------------------------------------------1
前言-------------------------------------------------------16
材料與方法------------------------------------------------ 18
結果-------------------------------------------------------29
討論-------------------------------------------------------42
結論-------------------------------------------------------47
參考文獻-------------------------------------------------- 48
表目錄
表一、魚類對錳之營養需求量-------------------------------15
表二、實驗飼料配方---------------------------------------19
表三、實驗飼料之一般成份---------------------------------20
表四、以不同錳含量飼料餵食吳郭魚稚魚八週後之增重百分率
、飼料效率及存活率---------------------------------------31
表五、以不同錳含量飼料餵食吳郭魚稚魚八週後之蛋白質利用
率、肝體比-----------------------------------------------32
表六、以不同錳含量飼料餵食吳郭魚稚魚八週後之體組成-------33
表七、以不同錳含量飼料餵食吳郭魚稚魚八週後之肝臟、魚體
、骨骼及肌肉中錳的濃度-----------------------------------34
表八、以不同錳含量飼料餵食吳郭魚稚魚八週後之肝臟MnSOD
的活性---------------------------------------------------35
表九、吳郭魚稚魚餵食不同劑量的含錳飼料八週後之全魚體
內錳含量與餵食含錳飼料之平衡關係-------------------------36
圖目錄
圖一、飼料中實際錳含量對於吳郭魚稚魚肝臟中MnSOD酵素活性之影
響。在酵素活性之迴歸曲線中breakpoint為6.83 mg/kg diet -------37
圖二、飼料中實際錳含量對於吳郭魚稚魚全魚錳含量的相關性。可得吳
郭魚對錳需求量為6.97 mg/kg diet,迴歸方程式為Y=-3.55X+24.73 (r=0.99)-----------------------------------------------------38
圖三、飼料中實際錳含量對於吳郭魚稚魚肝臟錳含量之影響。以直線迴
歸得一方程式為Y=0.02X+2.56(r=0.97)---------------------------39
圖四、飼料中實際錳含量對於吳郭魚稚魚骨骼錳含量之影響。以直線迴
歸得一方程式為Y=0.82X+71.13 (r=0.94)------------------------ 40
圖五、飼料中實際錳含量對於吳郭魚稚魚肌肉錳含量之影響。以直線迴
歸得一方程式為Y=0.01X+0.94 (r=0.93)------------------------- 41

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