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研究生:朱榮昌
研究生(外文):jung-chang chu
論文名稱:鯖魚內臟水解物作為Bacillussubtilis培養基氮源生產鹼性蛋白
論文名稱(外文):Mackerel Viscera Hydrolysate as Nitrogen Source of Bacillus subtilis Culture for Producing Alkaline Protease
指導教授:蕭泉源蕭泉源引用關係
指導教授(外文):chyuan-yuan shiau
學位類別:碩士
校院名稱:國立海洋大學
系所名稱:食品科學系碩士在職專班
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
中文關鍵詞:鯖魚內臟水解物醱酵
外文關鍵詞:mackerel viscera hydrolysatefermentation
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中文摘要
鯖魚內臟在50℃自家消化5小時後溶解度為65.5 %,水解液 pH 值下降,總氮 (TN)、非蛋白質態氮 (NPN)、游離胺基酸 (FAA)及氨含量則明顯增加,個別FAA中以牛磺酸 (Tau)、天冬胺酸 (Asp)、丙胺酸 (Ala)、麩胺酸 (Glu)、白胺酸 (Leu)、離胺酸 (Lys)等為主。與二種市售魚溶漿比較,TN、NPN與FAA皆較高。
Nutrient Broth (NB)與Luria Broth (LB)兩種培養基均能活化培養Bacillus subtilis CCRC 11062,但以LB為培養基在37℃、pH 7.5、8小時條件下培養效果較佳。比較不同氮源培養CCRC 11062生產鹼性蛋白,發現鯖魚內臟水解物培養效果僅次於酵母抽出物、Bacto- peptone,而優於麩胺酸、(NH4)2SO4、NH4Cl、(NH4)H2PO4及市售魚溶漿等。
鯖魚內臟水解物混合酵母抽出物、碳源、無機鹽類培養CCRC 11062生產鹼性蛋白,其最適培養基組成為: 鯖魚內臟水解物1.0 %、葡萄糖1.0 %、酵母抽出物 0.2 %及K2HPO4 0.05 %、MgSO4 0.04 %、Na2CO31.0 % 等,經利用2.5 L / 5 L醱酵槽,於溫度37℃、攪拌速率 500 rpm、通氣量1.0 vvm、pH 7.0條件下培養,可得酵素活性為2100 U / ml之鹼性蛋白。
鯖魚內臟水解物確能作為B. subtilis CCRC 11062菌株生長並代謝生產鹼性蛋白的氮源,而利用微生物的醱酵技術生產鹼性蛋白,有助於提昇魚類加工廢棄物的附加價值。
ABSTRACT
The solubility of mackerel viscera was 65.5 % after being hydrolyzed at 50℃ for 5hrs. The pH of the hydrolysate decreased with the progress of the reaction, while the amounts of total nitrogen (TN), non-protein nitrogen (NPN), free amino acid (FAA) and ammonia increased. The major FAA in the mackerel viscera hydrolysate were taurine (Tau), aspartic acid (Asp), alanine (Ala), glutamic acid (Glu), leucine (Leu), and lysine (Lys). As compared to two commercially available fish solubles, mackerel viscera hydrolysate had higher amounts of TN, NPN and FAA.
Bacillus subtilis CCRC 11062 was able to grow in both media of Nutrient Broth (NB)Medium and Luria Broth (LB)Medium, while it grow faster in the LB with the conditions of 37℃, pH 7.5 and 8 hrs. As nitrogen source of B. subtilis culture for the production of alkaline protease, the mackerel viscera hydrolysate had lower ability than yeast extract and Bacto-peptone, but higher than glutamic acid, (NH4)2SO4, (NH4)H2PO4, NH4Cl and commercial fish solubles.
In addition to mackerel viscera hydrolysate as nitrogen source, the culture medium of B. subtilis for producing alkaline protease contained yeast extract, carbon source, and inorganic salts. The most suitable ingredients in the medium included hydrolysate 1.0 %, glucose 1.0 %, yeast extract 0.2 %, K2HPO4 0.05 %, MgSO4 0.04 % and Na2CO3 1.0 %. Under the conditions of culture at 37℃, 500 rpm rotation , 1.0 vvm ventilation, and pH 7.0 in a 5-L fermenter with working volume of 2.5 L for 48 hrs, the alkaline protease with 2100 U/ml enzyme activity was obtained.
According to the results of this study, the mackerel viscera hydrolysate was able to serve as a nitrogen source for the growth of B. subtilis to produce the alkaline protease. The fish processing waste could be utilized efficiently through the fermentation technology of microorganism, and increased its additional value.
中文摘要………………………………………………………………….І
英文摘要………………………………………………………………...II
目錄……………………………………………………………………...III
壹、前言………………………………………………………………...1
貳、文獻整理………………………………………….…………......3
參、材料與方法………………………………………………………….19
肆、結果與討論………………………………………………………...29
伍、結論………………………………………………………………...43
陸、參考文獻…………………………………………………………...44
柒、表…………………………………………………………………….65
捌、圖…………………………………………………………………….83
陸、參考文獻
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