本研究目的為比較外生菌根，內生菌根與外生及內生菌根接種對尾葉桉幼苗生長之影響。本研究分別以硬皮馬勃外生菌根之菌絲體與刺繡球菌之孢子，分別接種至穴植管內人工培養土中生長4個月大之尾葉桉幼苗，接種一年後，即作各處理之比較分析。
尾葉桉幼苗經菌根菌接種後，對其生長皆具顯著促進效應。就苗高生長而言，依序為接種硬皮馬勃者>接種硬皮馬勃+刺繡球菌>接種刺繡球菌>未接種者，所有接種者皆與控制組呈顯著差異(P<0.05)。就地際根徑言，所有處理皆互不呈顯著差異(P>0.05)。就地上部乾重言，依序為接種硬皮馬勃+刺繡球菌>接種硬皮馬勃者>接種刺繡球菌>未接種者，且各接種處理皆與控制制組呈顯著差異(P<0.05)。就根系乾重言，依序為接種硬皮馬勃者>接種硬皮馬勃+刺繡球菌>接種刺繡球菌>未接種者，接種硬皮馬勃及雙重接種者與接種刺繡球菌或控制組互呈顯著差異(P<0.05)。就苗幹總體積言，依序為接種硬皮馬勃+刺繡球菌者>接種硬皮馬勃>接種刺繡球菌>未接種者，接種硬皮馬勃及雙重接種者與接種刺繡球菌及未接種者互呈顯著差異(P<0.05)。各處理之T/R率互不呈顯著差異(P>0.05)。尾葉桉與硬皮馬勃形成之菌根感染率較刺繡球菌者高，且互呈顯著差異。接種硬皮馬勃菌根之感染率與苗高生長呈顯著性正相關(r=0.97)。

The purpose of this study was to compare the influences of ecto- , endo- , and double-mycorrhizal inoculation on the growth of Eucalyptus urophylla Blake seedlings.Ectomycorrhizal mycelia of Scleroderma areolatum Her. and spores of Glomus spinosum Hu were used to inoculate to the 4-month old E.urophylla seedlings,which were cultured in the dibblings， tube containing artificial soil.One year after inoculation,comparative analysis was made among the treatments .
One year after inoculation,it showed that E.urophylla seedlings could from (1) ectomycorrhizae with S. areolatum , (2) endomycorrhizae with G. spinosum, and (3) double symbioses with the former both fungi.
The growth of E.urophylla seedlings was significantly stimulated (P<0.05) after being treated with mycorrhizal inoculation. In terms of height growth,the order was those inoculated with S.areolatum > S.areolatum + G.spinosum > those inoculated with G.spinosum.All inoculated seedlings were significant higher (P<0.05) than those of the uninoculated. In terms of root collar diameter,there was no significant difference (P>0.05) among all the treatments.In terms of top dry weight, the order was those inoculated with S.areolatum + G. spinosum > those inoculated with S.areolatum > those inoculated with G.spinosum > those of the uninoculated. All inoculated seedlings were significant higher (P<0.05) than those of the uninoculated. In terms of root dry weight, the order was those inoculated with S.areolatum > those inoculated with S.areolatum + G.spinosum > those inoculated with G.spinosum > those of the uninoculated. Those inoculated with S.areolatum and with the double-inoculated were significantly different (P<0.05) from those inoculated with G.spinosum only or those of the uninoculated. In terms of stem volume, the order was those inoculated with S.areolatum + G.spinosum > those inoculated with S. areolatum > those inoculated with G.spinosum > those with the uninoculated.The stem volume was significantly different (P<0.05) between those inoculated with S.areolatum,double inoculation and those with G.spinosum only or the uninoculated. T/R ratio was not significantly different among all the treatments.Mycorrhiza infection percentage formed by S. areolatum was significantly more (P<0.05) than formed by G. spinosum.Mycorrhiza infection percentage showed positive relationship (r=0.97) with seedlings height growth.

【目錄】
摘 要-------------------------------------------------------- Ⅰ
SUMMARY--------------------------------------------------------------------------------- III
目錄----------------------------------------------------------------------------- V
表目次----------------------------------------------------------------- VI
圖目次---------------------------------------------------------------- Ⅷ
壹、前言---------------------------------------------------------------------------- 1
貳、前人研究------------------------------------------------------------------------ 3
參、材料與方法--------------------------------------------------------------------- 21
一、尾葉桉之播種--------------------------------------------------------------- 21
二、菌根接種源之製備-------------------------------------------------------- 21
三、尾葉桉苗木之移植、接種及培育---------------------------------------- 22
四、苗木生長分析-------------------------------------------------------------- 23
五、苗木菌根感染率之測定---------------------------------------------------- 24
六、試驗設計及統計分析------------------------------------------------------- 26
七、研究流程圖---------------------------------------------------------------- 27
肆、結果------------------------------------------------------------------------ 28
一、地際根徑生長--------------------------------------------------- 28
二、苗高生長--------------------------------------------------------------------- 30
三、地上部及根系乾重----------------------------------------------------- 32
四、苗幹總體積--------------------------------------------------------------- 35
五、T/R率------------------------------------------------------------------ 36
六、菌根感染百分率------------------------------------------------------------ 37
七、各處理變數之相關性------------------------------------ 38
八、菌根在立體顯微鏡下之特徵--------------------------------------- 43
伍、討論---------------------------------------------------------------------------- 46
陸、結論---------------------------------------------------------------------------- 52
柒、參考文獻-------------------------------------------------------------------- 54
捌、附錄---------------------------------------------------------------------- 61
【表目次】
頁次
表2-1 華南地區桉樹外生菌根菌種類及分佈----------------------------------- 8
表2-2 華南地區桉樹VAM菌根菌種類及組成-------------------------------------- 9
表4-1 各處理對尾葉桉連月地際根徑生長之變方分析---------------------- 28
表4-2 各處理對尾葉桉連月地際根徑生長量(mm)之影響-------------- 30
表4-3 各處理對尾葉桉連月苗高生長之變方分析------------------------ 31
表4-4 各處理對尾葉桉連月地際根徑生長量(cm)之影響-------------------- 33
表4-5 經不同菌根菌接種處理尾葉桉苗木之地上部乾重變方分析----- 34
表4-6 經不同菌根菌接種處理尾葉桉苗木之根系乾重變方分析------ 34
表4-7 各處理對尾葉桉地上部及根系乾重、苗幹總體積、T/R率及菌根感染率之影響------------------------------------------------- 35
表4-8 經不同菌根菌接種處理尾葉桉苗木之苗幹總體積生長量變方分析------------------------------------------------------ 37
表4-9 經不同菌根菌接種處理尾葉桉苗木之T/R率變方分析-------- 38
表4-10 不同菌根菌接種處理尾葉桉苗木之菌根感染率(%)變方分析----------------------------------------------------------------------- 39
表4-11 尾葉桉苗木接種不同處理經12個月後之菌根感染百分率--------------- 40
表4-12 對照組之尾葉桉試驗培育12個月後生長量之相互相關係數-------- 41
表4-13 接種Sa之尾葉桉試驗培育12個月後感染率、生長量之相互相關係數------------------------------------------------------------------ 42
表4-14 接種Gs之尾葉桉試驗培育12個月後感染率、生長量之相互相關係數----------------------------------------------------------------- 43
表4-15 接種Sa+Gs之尾葉桉試驗培育12個月後感染率、生長量之相互相關係數----------------------------------------------------------------- 44
【圖目次】
頁次
圖2-1 外生菌根的主要形態--------------------------------------------------- 11
圖2-2 內生菌根的主要結構形態------------------------------------------------ 12
圖3-1 刺繡球菌孢子之外觀形態--------------------------------- 22
圖4-1 尾葉桉苗木接種龜紋硬皮馬勃、刺繡球菌、龜紋硬皮馬勃+刺繡球菌與對照組苗木試驗培育12個月後之根徑狀態比較------------- 29
圖4-2 尾尾葉桉苗木接種龜紋硬皮馬勃、刺繡球菌、龜紋硬皮馬勃+刺繡球菌與對照組苗木試驗培育12個月後之苗高生長狀態比較-------- 32
圖4-3 尾葉桉苗木接種龜紋硬皮馬勃、刺繡球菌、龜紋硬皮馬勃+刺繡球菌與對照組苗木試驗培育12個月後之根系狀態比較------------------- 36
圖4-4 尾葉桉苗木接種龜紋硬皮馬勃、刺繡球菌、龜紋硬皮馬勃+刺繡球菌與對照組苗木試驗培育12個月後之生長狀態比較--------------- 37
圖4-5 尾葉桉幼苗與硬皮馬勃形成之白色菌根形態------------------ 46
圖4-6 尾葉桉幼苗短根接種刺繡球菌初期所形成感染菌絲特徵-------- 46
圖4-7 尾葉桉幼苗接種刺繡球菌新根尚未感染與感染後之特徵------ 47
圖4-8 尾葉桉幼苗幼根構造經染色，所呈現囊狀體與胞外菌絲的特徵-- 47

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