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研究生:李文靜
研究生(外文):Wen-Ching Lee
論文名稱:蝴蝶蘭屬植物核醣體RNA基因的選殖及實質定位
論文名稱(外文):Cloning and physical mapping of rRNA genes in Phalaenopsis orchids
指導教授:高燕玉
指導教授(外文):Yen-Yu Kao
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:植物學研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:47
中文關鍵詞:蝴蝶蘭核醣體實質定位
外文關鍵詞:PhalaenopsisrRNA genesphysical mapping
相關次數:
  • 被引用被引用:4
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摘要
本實驗由原生種蝴蝶蘭P. aphrodite 中選殖出17~18S-5.8S-25~28S 及5S rDNA ,前者以 biotin — 16 - dUTP 標定,後者以 digoxigenin — 11 - dUTP 標定,利用雙重螢光原位雜交,同時定位在七種蝴蝶蘭原生種的染色體上。其中 P. aphrodite 有一對 17~18S-5.8S-25~28S rDNA 基因座和兩對 5S rDNA 基因座; P. mannii、P. cornu-cervi 及 P. lueddemanniana 各具一對 17~18S-5.8S-25~28S rDNA 基因座及5S rDNA 基因座;P. amboinensis 有七對17~18S-5.8S-25~28S rDNA 基因座及一對 5S rDNA 基因座; P. venosa 有五對 17~18S-5.8S-25~28S rDNA 基因座及一對 5S rDNA 基因座; P. violacea 有四對 17~18S-5.8S-25~28S rDNA 基因座及兩對 5S rDNA 基因座。根據傳統分類、細胞遺傳學證據以及 rDNA 實質定位的結果,推測 P. lueddemanniana、P. mannii及P. cornu-cervi 的親緣關係似乎較為接近,而 P. amboinensis、P. venosa 及 P. violacea 之 17~18S-5.8S-25~28S rDNA 幾乎都位於小染色體上,現象特殊,而這些基因是否表現尚待進一步的研究。
Abstract
DNA fragments 17~18S - 5.8S — 25~28S and 5S rDNA were cloned from Phalaenopsis aphrodite. The chromosomal localization of these two rDNAs in seven Phalaenopsis species was determined by double target fluorescence in situ hybridization. In this study, we detected (1) two 17~18S - 5.8S — 25~28S rDNA loci and four 5S rDNA loci in P. aphrodite; (2) two 17~18S - 5.8S — 25~28S rDNA loci and two 5S rDNA loci were in P. mannii, P. cornu-cervi and P. lueddemanniana; (3) fourteen 17~18S - 5.8S — 25~28S rDNA loci and two 5S rDNA loci in P. amboinensis; (4) ten 17~18S - 5.8S — 25~28S rDNA loci and two 5S rDNA loci in P. venosa; (5) eight 17~18S - 5.8S — 25~28S rDNA loci and four 5S rDNA loci in P. violacea. P. mannii, P. cornu-cervi and P. lueddemanniana were considered to be closely related according to traditional classification, cytogenetic data and physical mapping of these two rDNAs. Almost all the 17~18S - 5.8S — 25~28S rDNA loci were found in small chromosomes of P. amboinensis, P. venosa and P. violacea. Whether these genes are expressed remains further investigation.
目錄
中文摘要--------------------------------------------------i
英文摘要-------------------------------------------------ii
簡寫字對照表--------------------------------------------iii
壹、前言--------------------------------------------------1
貳、材料與方法
一、植物材料------------------------------------------9
二、DNA 的抽取----------------------------------------9
三、rDNA 選殖體的篩選---------------------------------10
四、螢光原位雜交--------------------------------------18
參、結果
一、rDNA 選殖體的篩選、定序與分析---------------------25
二、螢光原位雜交--------------------------------------26
肆、討論--------------------------------------------------38
伍、參考文獻----------------------------------------------41
伍、參考文獻
李宜學. 2000. 利用螢光原位雜交探討微衛星體序列在蝴蝶蘭的實質分佈. 國立台灣大學植物學研究所碩士論文.
高玉馨. 2001. 以 5S rDNA 基因間空白區序列探討蝴蝶蘭屬植物之親緣關係. 國立台灣大學植物學研究所碩士論文.
Adams, S. P., I. J. Leitch, M. D. Bennett, M. W. Chase, and A. R. Leitch. 2000. Ribosomal DNA evolution and phylogeny in Aloe (Asphodelaceae). Am. J. Bot. 87: 1578-1583.
Ali, H. B. M., A. Meister, and I. Schubert. 2000. DNA content, rDNA loci, and DAPI bands reflect the phylogenetic distance between Lathyrus species. Genome 43: 1027-1032.
Appels, R., C. Driscoll, and W. J. Peacock. 1978. Heterochromatin and highly repeated DNA sequences in rye (Secale cereale). Chromosoma 70: 67-89.
Arends, J. C. 1970. Cytological observations on genome homology in eight interspecific hybrids of Phalaenopsis. Genetica 41: 88-100.
Ausubel, F. M., R. Brent, D. D. More, J. G. Sediman, J. A. Smith, and K. Struhl. 1987. Current Protocols in Molecular Biology. Greene Publishing Associates and Wile-Interscience USA.
Badaeva, E. D., B. Friebe, and B. S. Gill. 1996. Genome differentiation in Aegilops. 2. Physical mapping of 5S and 18S-26S ribosomal RNA gene families in diploid species. Genome 39: 1150-1158.
Chung, M. C., C. N. Ning, and H. K. Wu. 1993. Localization of ribosomal RNA genes on rice chromosomes. Bot. Bull. Acad. Sin. 34: 43-55.
D’Hont A., D. Ison, K. Alix, C. Roux, and J. C. Glaszmann. 1998. Determination of basic chromosome numbers in the genus Saccharum by physical mapping of ribosomal RNA genes. Genome 41: 221-225.
Dressler, R. L. 1993. Phylogeny and classification of the orchid family. Cambridge University Press, Cambridge.
Flavell, R. B. 1985. Repeated sequences and genome change. In: B. Hohn and E.S. Dennis (eds) Genetic Flux in Plants. Springer-Verlag, Wien, New York. pp. 139-156.
Flavell, R. B. 1986a. Repetitive DNA and chromosome evolution in plants. Phil. Trans. R. Soc. Lond. B, 312: 227-242.
Flavell, R. B. 1986b. The structure and control of expression of ribosomal RNA genes. Oxford Surv. Plant Mol. Cell Biol. 6: 251-274.
Fu Y. M., W. H. Chen, W. T. Tsai, Y. S. Lin, M. S. Ghyou, and Y. H. Chen. 1997. Phylogenetic studies of taxonomy and evolution among wild species of Phalaenopsis by random amplified polymorphic DNA markers. Rept. Taiwan Sugar Res. Inst. 157: 27-42.
Fuchs, J., M. Kűhne, and I. Schubert. 1998. Assignment of linkage groups to pea chromosomes after karyotyping and gene mapping by fluorescence in situ hybridization. Chromosoma 107: 272-276.
Gall, J. G., and C. Pardue. 1969. Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc. Natl. Acad. Sci. U. S. A. 63: 378-383.
Gawel, N. J., and R. L. Jarret. 1991. A modified CTAB DNA extraction procedure for Musa and Ipomoea. Plant Mol. Biol. Reptr. 9: 262-266.
Hasterok, R., G. Jenkins, T. Langdon, and R. N. Jones. 2001. Ribosomal DNA is an effective marker of Brassica chromosomes. Theor. Appl. Genet. 103: 486-490.
Heslop-Harrison, J. S. 1991. The molecular cytogenetics of plants. J. Cell Sci. 100: 15-21.
Hubbell, H. R. 1985. Silver staining as an indicator of active ribosomal genes. Stain Technol. 60: 285-294.
Kao, Y. Y., S. B. Chang, T. Y. Lin, C. H. Hsieh, Y. H. Chen, W. H. Chen, and C. C. Chen. 2001. Differential accumulation of hetrochromatin as a cause for karyotype variation in Phalaenopsis orchids. Ann. Bot. 87: 387-395.
Kreike, C. M., J. R. A. de Koning, and F. A. Krens. 1990. Nonradioactive detection of single-copy DNA-DNA hybrids. Plant Mol. Biol. Reptr. 8: 172-179.
Kubis, S., T. Schmidt, and J. S. Heslop-Harrison. 1998. Repetitive DNA elements as a major component of plant gemones. Ann. Bot. 82 (Supplement A): 45-55.
Lapitan, N. L. V. 1992. Organization and evolution of higher plant nuclear genomes. Genome 35: 171-181.
Leitch, A. R., T. Schwarzacher, D. Jackson, and I. J. Leitch. 1994. In Situ Hybridization: a Practical Guide. BIOS Scientific Publishers, Oxford, UK.
Leitch, I. J., and J. S. Heslop-Harrison. 1993. Physical mapping of four sites of 5S rDNA sequences and one site of the α-amylase-2 gene in barley(Hordeum vulgare). Genome 36: 517-523.
Lin, S., H. C. Lee, W. H. Chen, C. C. Chen, Y. Y. Kao, Y. M. Fu, Y. H. Chen, and T. Y. Lin. 2001. Nuclear DNA contents of Phalaenopsis sp. and Doritis pulcherrima. J. Amer. Soc. Hort. Sci. 126: 195-199.
Mukai, Y., T. R. Endo, and B. S. Gill. 1991. Physical mapping of the 18.26 rRNA multigene family in common wheat: identification of a new locus. Chromosoma 100: 71-78.
Murata, M, J. S. Heslop-Harrison, and F. Motoyoshi. 1997. Physical mapping of the 5S ribosomal RNA genes in Arabidopsis thaliana by multi-color fluorescence in situ hybridization with cosmid clones. Plant J. 12: 31-37.
Osuji, O. J., J. Crouch, G. Harrison, and J. S. Heslop-Harrison. 1998. Molecular cytogenetics of Musa species, cultivars and hybrids: location of 18S-5.8S-25S and 5S rDNA and telomere-like sequences. Ann. Bot. 82: 243-248.
Panaud, O., G. Magpantay, and S. McCouch. 1993. A protocol for nonradioactive DNA labeling and detection in the RFLP analysis of rice and tomato using single-copy probes. Plant Mol. Biol. Reptr. 11: 54-59.
Pardue, M. L., S. A. Gerbi, R. A. Eckhardt, J. G. Gall. 1970. Cytological localization of DNA complementary to ribosomal RNA in polytene chromosomes of Diptera. Chromosoma 29: 268-290.
Porebski, S., L. G. Bailey, and B. R. Baum. 1997. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol. Biol. Reptr. 15: 8-15.
Sanger, F. 1981. Determination of nucleotide sequences in DNA. Science 214: 1205-1210.
Sagawa, Y. 1962. Cytological studies of the genus Phalaenopsis. Am. Orchid Soc. Bull. 31: 459-465.
Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Shindo, K., and H. Kamemoto. 1963. Karyotype analysis of some species of Phalaenopsis. Cytologia 28: 390-398.
Sweet, H. R. 1980. The genus Phalaenopsis. Day Printing Corp., Pomon, California.
Speicher, M. R., S. G. Ballard, and D. C. Ward. 1996. Karyotyping human chromosomes by combinational multi-fluor FISH. Nat.Genet. 12: 368-375.
Trask, B. J. 1991. Fluorescence in situ hybridization: applications in cytogenetics and gene mapping. Trends Genet. 7: 149-154.
Westneat, D. F., W. A. Noon, H. K. Reeve, and C. F. Aquadro. 1988. Improved hybridization conditions for DNA “ fingerprints “ probed with M13. Nucleic Acids Res. 16: 4161.
Woodard,J. W. 1951. Some chromosome numbers in Phalaenopsis. Am. Orchid Soc. Bull. 20: 356-358.
Zoldos, V., D. Papes, M. Cerbah, O. Panaud, V. Besendorfer, and S. Siljak-Yakovlev. 1999. Molecular-cytogenetic studies of ribosomal genes and heterochromatin reveal conserved genome organization among 11 Quercus species. Theor. Appl. Genet. 99: 969-977.
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