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研究生:游大慶
研究生(外文):Yu, Ta-Ching
論文名稱:EB病毒核蛋白EBNA-1對試管內轉譯及PKR功能影響之探討
論文名稱(外文):Analysis of the Effect of EBV Nuclear Antigen 1 (EBNA-1) on in vitro Translation and PKR Function
指導教授:陳美如陳美如引用關係
指導教授(外文):Chen, Mei-Ru
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:微生物學研究所
學門:生命科學學門
學類:微生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:64
中文關鍵詞:EB病毒核蛋白試管內轉譯PKR功能
外文關鍵詞:EBNA-1in vitro translationPKR function
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EBNA-1是一個具有多元化功能的蛋白質,其已知的生物功能包括促進EB病毒潛伏期DNA的複製及維持EB病毒以質體型式穩定存在於細胞中。EBNA-1也是唯一會持續在所有與EB病毒相關惡性腫瘤中表現的病毒核抗原。在EBNA-1的胺基酸序列中,發現其帶有3個與RNA結合有關的RGG motifs,並經由RNA homopolymer binding assay實驗證明RGG motifs對於EBNA-1結合RNA的功能是重要的。為了進一步瞭解EBNA-1結合RNA在細胞內可能之生物功能,我們首先利用桿狀病毒系統表現的重組EBNA-1 (rEBNA-1),探討其對3種5’端不同結構的luciferase RNA進行體外轉譯時的影響。結果顯示EBNA-1可在次佳的體外轉譯條件下,增加3種5’端不同結構RNA之體外轉譯效率。其次因為許多具有RNA結合能力的病毒蛋白常藉由抑制PKR功能幫助病毒複製,因此進一步探討EBNA-1是否會影響PKR的活性。我們發現rEBNA-1可回復poly(I)(C)引起之體外轉譯抑制現象。進一步在免疫沉澱-蛋白質激酶活性實驗中,則發現rEBNA-1會抑制PKR自我磷酸化,同時也觀察到rEBNA-1有被磷酸化情形。

The multi-functional Epstein-Barr virus nuclear antigen 1 (EBNA-1) has been shown to play critical roles in both viral replication and maintenance of the viral episome. EBNA-1 is the only latent viral nuclear antigen consistently expressed in EBV-associated malignancies. There are three RGG motifs with putative RNA binding activities in EBNA-1. By using RNA homopolymer binding assay, we demonstrated that RGG motifs are essential for RNA binding of EBNA-1 in a previous study. In order to further study possible biological function(s) of this RNA binding ability, we first analyzed the effect of baculovirus expressed recombinant EBNA-1 (rEBNA-1) on in vitro translation of luciferase RNA with different 5’ non-coding sequences. We found that EBNA-1 was able to stimulate translation efficiency of different templates under a sub-optimal in vitro translation condition. Since many viral RNA binding proteins help virus replication through inhibiting PKR function, experiments were performed to examine whether EBNA-1 could inhibit PKR activity. rEBNA-1 was found to rescue the poly(I)(C) induced inhibition of in vitro translation activity. In addition, PKR auto- phosphorylation in immunoprecipitaion kinase assay was partly inhibited by rEBNA-1, whereas in vitro phosphorylation of rEBNA-1 was observed at the same time.

中文摘要 ------------------------------------------- I
英文摘要 ------------------------------------------- II
緒論 ----------------------------------------------- 1
材料與方法 ------------------------------------------12
實驗結果 --------------------------------------------18
討論 ------------------------------------------------26
圖表 ------------------------------------------------32
附錄 ------------------------------------------------48
參考文獻 --------------------------------------------54

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