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研究生:張家榮
研究生(外文):Chang Chia Jung
論文名稱:兩性次微米高分子粒子之合成與鑑定
論文名稱(外文):Synthesis and characterization of amphoteric submicron polymer particles
指導教授:陳崇賢陳崇賢引用關係
指導教授(外文):C.S. Chern
學位類別:碩士
校院名稱:國立臺灣科技大學
系所名稱:化學工程系
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:139
中文關鍵詞:批次無乳化聚合反應半批次種子無乳化聚合反應
外文關鍵詞:batch soap-free emulsion polymerizationbatch soap-free emulsion polymerization
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本研究利用批次無乳化聚合反應製備種子,再利用半批次種子無乳化聚合反應來合成乳漿粒子,由於配方中加入 AAP、MAA 濃度不同,使得乳漿粒子表面帶有正 (或負) 電性,並使粒子具有 pH 敏感性。當 pH 增加時,粒子表面 ζ 之絕對值隨著 [MAA] 增加亦隨之增加,當 pH 減小時,粒子表面的 ζ 隨著 [AAP] 增加亦隨之增加。
藉由乳漿粒子分別與Lysozyme、Pepsin、Hemoglobin進行恆溫吸附以觀察粒子粒徑變化及表面的電性,發現隨著改變不同的溶液環境,乳漿粒子與三種蛋白質吸附後所呈現的性質亦會不同。進一步利用乳漿粒子對 Lysozyme、Pepsin 恆溫吸附行為顯示,在 pH = 3 時,Pepsin 分子吸附在粒子表面上,而 Lysozyme 不會產生吸附現象;在 pH = 7 時, Lysozyme 分子藉由靜電作用力吸附在乳漿粒子 (C2-1)上,Pepsin 反而會藉著疏水作用力吸附在粒子上;至於在 pH = 9.5 時,Lysozyme 分子吸附在粒子上,而 Pepsin 反而不會吸附在其上。

The seed latex particles were prepared by batch soap-free emulsion polymerization. The latex products were then synthetized by semibatch seeded soap-free emulsion polymerization. As a result of different AAP and MAA concentrations in the recipes used, latex particles carrying different charges and displaying pH-sensitivity were obtained. At high pH, the Zeta potential of latex particles (ζ) increases with increasing [MAA]. At low pH , ζ increases with increasing [AAP].
Isothermal adsorption of latex particles with Lysozyme, Pepsin, or Hemoglobin were carried out. When the aqueous solution environment was varied, the properties of the adsorbed latex particles were quite different. At pH = 3, Pepsin adsorbed on the particle surfaces, but Lysozyme could not adsorb on the particle surfaces. At pH = 7, Lysozyme adsorbed on latex particle surfaces (C2-1) by electrostatic interaction, whereas Pepsin adsorbed on latex particle surfaces by hydrophobic interaction. At pH = 9.5, Lysozyme adsorbed on particle surfaces, whereas Pepsin was incapable of adsorbing.

中文摘要…………………………………………………………………….i
英文摘要…………………………………………………………………….ii
誌謝………………………………………………………………………….iii
目錄………………………………………………………………………….iv
圖目錄………………………………………………………………………viii
表目錄………………………………………………………………………..xi
第一章 緒論………………………………………………………………..1
1-1 研究背景及目的………………………………………………….1
1-2 研究內容簡介…………………………………………………….1
第二章 文獻回顧…………………………………………………………..3
2-1 無乳化聚合反應簡介……………………………………………….3
2-1-1 無乳化聚合反應的成核機構…………………………………..4
2-1-2 種子聚合反應…………………………………………………..5
2-2 膠體溶液穩定性………………………………………………….6
2-2-1 電雙層理論……………………………………………………..6
2-2-2 膠體粒子表面帶電原因………………………………………..7
2-2-3 DLVO 理論…………………………………………………….8
2-3 高分子對膠體溶液穩定性之影響…………………………………12
2-4 膠體粒子的失穩定性………………………………………...17
2-5 高分子聚合體粒子在生化分離上之應用…………………………18
2-5-1 聚合體粒子和蛋白質之分離機構…………………………..19
2-5-2 聚合體粒子吸附載體的設計………………………………..21
2-5-3 聚合體粒子在生化分離上之文獻化分離上之文獻………..27
2-6 本研究室在蛋白質純化上之研究…………………………………..34
第三章 實驗藥品、設備及方法…………………………………………..38
3-1 藥品………………………………………………………………....38
3-2 實驗儀器及設備……………………………………………………40
3-3 實驗方法……………………………………………………………41
3-3-1 乳漿之製備……………………………………………………41
3-3-2 乳漿之清洗步驟………………………………………………46
3-3-3 利用TEM 測量乳漿粒子粒徑………………………………47
3-3-4 電導度滴定 ( Conductometric Titrations)…………………... 47
3-3-5 pH 值對乳漿粒子表面電位 (ζ) 之影響…………………..48
3-3-6 不同 pH 值對乳漿粒子 (dP) 之影響……………………….48
3-3-7 不同 NaCl 濃度對乳漿粒子表面電位 (ζ) 之影響……….49
3-3-8 不同 NaCl 濃度對乳漿粒子粒徑 (dP) 之影響……………..49
3-3-9 以元素分析法測量高分子粒子中的氮含量…………………50
3-3-10 乳漿粒子對 NaCl 濃度之凝聚動力學實驗…………………50
3-3-11 加入的乳漿產品 (A3) 來測試乳漿粒子之性質…………….50
3-3-12 加入不同濃度蛋白質對乳漿粒子表面電位及粒徑之影響………………………………………………………………51
3-3-13 Lysozyme 及 Pepsin 濃度檢量線之測定………………….51
3-3-14 乳漿粒子對 lysozyme 之恆溫吸附實驗…………………...52
3-3-15 乳漿粒子對 pepsin 之恆溫吸附實驗………………………52
第四章 結果與討論……………………………………………………….61
4-1 乳漿粒子之合成製備及特性………………………………………61
4-1-1 乳漿之製備……………………………………………………61
4-1-2 乳漿粒子上所帶之胺基含量…………………………………62
4-1-3 乳漿粒子中羧基的分佈………………………………………63
4-1-4 pH 值對乳漿粒子之 Zeta 電位 (ζ) 之影響………………65
4-1-5 pH 對乳漿粒子粒徑 (dp) 之影響……………………………67
4-1-6 NaCl 濃度對乳漿粒子之 Zeta 電位 (ζ) 及粒徑 (dp) 之影響
…………………………………………………………………..68
4-1-7 NaCl 濃度對乳漿粒子之凝聚動力探討……………………...69
4-2 乳漿粒子之鑑定……………………………………………………72
4-2-1 利用乳漿粒子 (A3) 測試乳漿粒子之性質………………….72
4-2-2 不同蛋白質濃度對乳漿粒子 ζ 電位及粒子粒徑 (dp) 之影響
………………………………………………………………...73
4-2-3 乳漿粒子對蛋白質恆溫吸附行為…………………………..78
第五章 結論與建議……………………………………………………...126
5-1 結論………………………………………………………………..126
5-1-1 乳漿粒子之合成製備及特性…………………………………126
5-1-2 乳漿粒子之鑑定………………………………………………126
5-1-2-1不同蛋白質濃度對乳漿粒子之電性交互作用影響……..126
5-1-2-2乳漿粒子對蛋白質之交互作用…………………………..127
5-2 建議………………………………………………………………..128
參考文獻…………………………………………………………………...129
附錄………………………………………………………………………...137

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