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研究生:白福祥
研究生(外文):Fu-Hsiang Pai
論文名稱:Paclobutrazol前處理對甘藷耐寒性之影響
論文名稱(外文):The Effect of Paclobutrazol Pretreatment on the Chilling Tolerance of Sweet Potato (Ipomoea batatas(L.) Lam.)
指導教授:羅筱鳳
指導教授(外文):Hsiao-Feng Lo
學位類別:碩士
校院名稱:中國文化大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:104
中文關鍵詞:低溫逆境低溫耐受性甘藷抗氧化系統自由基
外文關鍵詞:chilling stresschilling tolerancesweet potatoantioxidant systemfree radicalPaclobutrazolPP333
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甘藷(Ipomoea batatas ( L.) Lam.)為重要雜糧作物,氣溫低於10℃時發生寒害。試驗一根據22個甘藷品種在5℃生長14天後之外表形態,以及24個甘藷品種在常溫(24/20℃)1天、低溫(7/7℃)1、3、5天和回溫(24/20℃)1、3天之葉片葉綠素含量(CHL)、Malondialdehyde含量(MDA)、葉綠素螢光反應、電解質滲漏(Leakiness)與相對含水量(RWC),篩選出台灣經濟栽培品種耐寒‘台農71號’與不耐寒‘台農65號’做為試驗二及試驗三之材料。
試驗二亦根據上述常溫、低溫5天及回溫3天期間六項參數,測試‘台農65號’ PBZ前處理之適當濃度,以葉面噴施300μg/5ml/plant為佳。
試驗三‘台農71號‘和‘台農65號‘於常溫(24/20℃)下葉面噴施PBZ 300μg/ 5mL/ plant及dH2O 5mL/ plant後24hr,予以低溫(7/7℃)5天及回溫(24/20℃)3天處理,期間測定葉片之膨壓(TP)、相對含水量、葉綠素含量、葉綠素螢光、電解質滲漏、Ascorbate peroxidase (APX)和glutathione reductase (GR)活性、以及Ascorbate (ASA)與Glutathione含量。在低溫與回溫期間,’台農65號’比’台農71號’有較高之CHL含量、APX與GR活性及GSH/ GSSG值,而’台農71號’則比’台農65號’有較佳之TP、RWC、Leakiness、Fv/Fm、Fo及ΦPSⅡ,’台農71號’確較耐寒。
在低溫前葉面噴施PBZ 300μg/5mL/plant僅增加耐寒品種’台農71號’於低溫及回溫期間之ASA含量。而不耐寒’台農65號’在噴施PBZ後,低溫及回溫期間TP、CHL及還原劑GSH與ASA含量增加,Leakiness和Fo亦減小,但APX及GR活性降低。

Sweet potato is an important food crop. Temperatures below 10℃ cause chilling injury of sweet potato. In Experiment 1, the appearance of 22 varieties at 5℃ for 14 days was observed. Leaf chlorophyll (CHL) and malondialdehyde contents, leakiness, chlorophyll fluorescence and relative water content (RWC), of 24 varieties were measured during 5 days of chilling (7/ 7℃) and the following 3 days of recovery (24/20℃). Taiwan cultivated chilling-tolerant variety ‘Tainung 71’ and chilling-sensitive ‘Tainung 65’ were selected as experimental materials of Experiment 2 and 3.
In Experiment 2, based on the mentioned six parameters during 5-days chilling (7/ 7℃) and the following 3-days recovery (24/20℃), PBZ pretreatment concentration of ‘Tainung 65’ was measured. The optimal concentration was 300μg/5mL/plant by leaf spray.
In Experiment 3, turgor pressure (TP), RWC, CHL, chlorophyll fluorescence, leakiness, ascorbate peroxidase (APX) and glutathione reductase (GR) activity, ascorbate (ASA) and glutathione contents of ‘Tainung 71’ and ‘Tainung 65’ leaves during 5 days chilling and 3 days recovery were measured after 300μg/5mL /plant PBZ or 5mL/plant d H2O of leaf spray at 24hr before chilling. ‘Tainung 65’ possessed higher CHL content, APX and GR activity, and GSH/GSSG during chilling and recovery. While ‘Tainung 71’ exhibited better RWC, Leakiness, Fv/Fm, Fo, ΦPSⅡ than ‘Tainung 65’. ‘Tainung 71’ was more chilling-tolerant.
Pre-spray of PBZ 300μg/5mL/plant only promoted ASA content of chilling -tolerant ‘Tainung 71’ during chilling and recovery; but increased TP, CHL and reducing agents GSH and ASA, also inhibited Leakiness and Fo of ‘Tainung 65’. While APX and GR activity of ‘Tainung 65’ were lowered.

中文摘要………….………………………………………………….....1
壹、前言……………………………………………………………….….2
貳、前人研究………………………………………………………………4
一. 甘藷近期發展………………………………………….....…4
二. 溫度對甘藷生產之影響.………………………………….….4
三. 寒害對植物生長之影響….……….…………………….....6
四. 寒害對植物生理之影響………………...………………..…8
(一)細胞膜結構改變……………………………..….…………..….…8
(二)光合作用………………………….…………………………..…...9
(三)葉綠素螢光………………………………………..…………..…..9
(四)活化氧族之產生….………….…………………………………...11
五. 植物的抗氧化系統…….……………………………….....12
六. Paclobutrazol的之生理作用…….…………………..……13
七. Paclobutrazol減少氧化逆境傷害………….………………15
八. Paclobutrazol對環境之影響…….………………………..15
參、材料與方法....…………………………………………….…....17
一、耐寒性品種篩選.………………………….……………….…....17
(一)低溫之外表形態….………………….………………...……....17
(二)低溫下之生理變化……………………….………………...….…17
二、Paclobutrazol濃度測驗….……………………………………...18
(一)’桃園2號’ Paclobutrazol 處理濃度試驗….……………18
(二)’台農65號’Paclobutrazol處理濃度試驗….…………..…….19
三、Paclobutrazol前處理對甘藷低溫生理之影響…...…………...19
四、測定項目….………………………...……………………….....21
(一)葉片膨壓 ( turgor pressure )……………………………….…21
(二)相對含水量 ( relative water content ).…..……………...21
(三)電解質滲漏測定 ( Leakiness ) .……………..………….....21
(四)脂質過氧化 ( Lipid peroxidation )…………..……………..22
(五)葉綠素含量 ( chlorophyll content ).……….……………….22
(六)葉綠素螢光 ( chlorophyll fluorescence ) ..………….…..22
(七)蛋白質萃取…….……………………………………………..…..23
(八)Ascorbate peroxidase ( APX )活性 .…..…………………….24
(九)Glutathione reductase ( GR )活性…………………………...24
(十)Ascorbate含量 ……….………………………………….……...25
(十一)Glutathione含量….…………………………………..……….25
肆、試驗結果…………………………………….…………………..…28
試驗一、耐寒性品種篩選….……………………………………..…..28
(一)低溫之外表形態變化……………………….…………..……28
(二)低溫下之生理變化.. .……………………….………..…..28
試驗二、Paclobutrazol濃度測驗………………………………………33
(一)’桃園2號’ Paclobutrazol 處理濃度試驗….……...….33
(二)’台農65號’Paclobutrazol處理濃度試驗.…………….………33
三、Paclobutrazol前處理對甘藷低溫生理之影響.………………….35
伍、討論….………………………………………………………………42
一、耐寒性品種篩選….………………………………………………..42
二、Paclobutrazol濃度測驗…….………………….………………..44
三、PBZ前處理對甘藷低溫生理之影響…..………………………....45
(一)低溫對植物水分吸收之影響…..…………………...……..45
(二)低溫對植物細胞電解質滲漏之影響.…………………….….46
(三)低溫對植物光合作用之影響………………………………….47
(四)低溫對甘藷ascorbate-glutathione cycle的影響.……….50
陸、結論….. …………………………………………………...…….57
圖……………. ………………………………......………………….58
表….……………………………………………….…………………….63
引用文獻……………. ……………………..……………………..….96
英文摘要……….………………………………………………….……105

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