(3.238.186.43) 您好!臺灣時間:2021/02/25 01:54
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:劉清輝
論文名稱:壬基苯酚對牛腎上腺髓質嗜鉻細胞鈣恆定之影響
論文名稱(外文):Effects of nonylphenol on Ca2+ mobilization in bovine adrenal chromaffin cells
指導教授:劉佩珊劉佩珊引用關係
學位類別:碩士
校院名稱:東吳大學
系所名稱:微生物學系
學門:生命科學學門
學類:微生物學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:56
中文關鍵詞:壬基苯酚環境荷爾蒙牛腎上腺嗜鉻細胞鈣離子濃度尼古丁受器
相關次數:
  • 被引用被引用:4
  • 點閱點閱:67
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:1
動情素已知除了會活化細胞內的動情素受器引發基因型效應外,也可作用在膜上的離子通道而引起神經衝動快速改變; 其中的雌二醇被發現可影響包括尼古丁受器等膜蛋白的活性。而壬基苯酚(NP) 則是在塑膠工業上的常見污染物質,它可經由動情素受器引發似動情素的基因性效應。本篇文章探討了壬基苯酚對牛腎上腺髓質嗜鉻細胞內鈣離子濃度這個非基因性效應的影響。實驗結果發現壬基苯酚可抑制與細胞膜上的尼古丁受器(IC50 為0.9 μM)、膜電位敏感型鈣離子通道(IC50 為2.2 μM)相連結的鈣訊,這顯示出壬基苯酚可干擾此兩類膜蛋白之功能,而它在尼古丁受器的抑制作用是屬於非競爭性且具可逆性; 此外,壬基苯酚本身並可引起內質網貯鈣的釋出。綜合而言,壬基苯酚可釋出內質網貯鈣以及抑制與尼古丁受器、膜電位敏感型鈣離子通道相連結的鈣訊而干擾到細胞內的鈣離子濃度之恆定。
In addition to exerting genomic effects via intracellular steroid receptors, estrogen exerts nongenomic effects through interactions with membrane ion channels to lead the rapid alteration of neuronal excitability. Estradiol is known as to address the modulation the activities of membrane proteins, including nicotinic acetylcholine receptors (nAChR). Nonylphenol (NP), a common contaminant found in plastics and organic solvents, exerts genomic estrogenic-like effects via estrogen receptors. We investigated the possibility of NP exerting nongenomic estrogenic-like effects on calcium mobilization in bovine adrenal chromaffin cells. Our results show that NP inhibited Ca2+ signaling induced by high K+ solution (IC50 2.2 μM) as well as by the nicotinic ligand l, 1,1-dimethyl-4-phenyl- piperazinium iodide (DMPP) (IC50 0.9 μM). We therefore suggest that both nAChR and voltage-gated Ca2+ channels are addressing sites of NP on the plasma membrane. Its inhibitory effect on nAChR was found to be both noncompetitive and reversible, remaining unchanged as nAChR ligand concentration increased and decreasing after washing. In addition the above effects, NP itself can induce a calcium transient in the presence or absence of external calcium. Thapsigargin treatment will decrease the calcium transient induced by NP; this illustrates that NP release calcium from endoplasmic reticulum. We conclude that NP can interfere calcium mobilization, including it releasing internal calcium and inhibiting the calcium responses coupled with nAChR and voltage-gated ion channels.
縮寫表Ι
中文摘要II
英文摘要III
前言1
前人文獻回顧3
材料與方法9
結果14
討論21
參考文獻31
圖表36
Barker, J. L., Harrison, N. L., Lange, G. D., and Owen, D. G., (1987) Potentiation of -aminobutyric-acid-activated chloride conductance by a steroid anaesthetic in cultured rat spinal neurones. J. Physiol. 386,485-501.
Bergerone R., de Montigny, C., and Debonnel. G., (1996) Poytentiation of neuronal NMDA response induced by dehydroepiandosterone and its suppression by progesterone: effects mediated via sigma receptors. J. Neurosci. 16,1193-1202.
Cottrell, G. A., Lambert, J. J., and Peters, J. A., (1987) Modulation of GABAA receptor acitvity by alphaxalone. Br. J. Pharmacol. 90,491-501.
Demirgoren, S., Majewska, M. D., Spivak, C.E., London,E.D., (1991) Receptor binding and electrophysiological effects of dehydroepiandrosterone sulfate, an antagonist of the GABAA receptor. Neuroscience.45,127-35
Harrison N. L., Vicini, S., and Barker, J. L., (1987) A steroid anaesthetic prolongs inhibitory postsynaptic currents in cultured rat hippocampal neurons. J. Neurosci. 7,604-609.
Irwin, R.P., Lin, S.Z., Rogawski,M.A., Purdy, R.H., and Paul, S.M., (1994) Steroid potentiation and inhibition of N-methyl-D-aspartate receptor-mediated intracellular Ca2+ responses: structure-activity studies. J-Pharmacol-Exp-Ther. 271, 677-82
Irwin, R.P. and et al., (1992) Pregnenolone sulfate augments NMDA receptor mediated increases in intracellular Ca2+ in cultured rat hippocampal neurons. Neurosci-Lett. 141,30-4
Ke, L., and Lukas, R.J. (1996) Effects of steroid exposure on ligand binding and functional activities of diverse nicotinic acetylcholine receptor subtypes. J-Neurochem. 67,1100-12
Liu P.-S., Lin, M.-K. and Hsieh, H.-L. (1996) DHEAS inhibition of catecholamine secretion from bovine adreanl chromaffin cells. Neurosci. Let. 204,181-184.
Lopez, M. G., et al., (1991) Membrane-mediated effects of the steroid 17─estradiol on adrenal catecholamine release. J. Pharmacol. Exp. Ther. 259,279-285.
Majewska, M. D., Demirogorn, S., Spivak, C. E., and London, E. D., (1990), Binding of pregnenolone sulfate to rat brain membranes suggests multiple sites of steroid action at the GABAA receptor. Eur. J. Pharmamacol. 189,307-315.
Majewska, M. D. and Schwartz, R. D. (1987) Pregnenolone sulfate: an endogenous antagonist of the g-aminobutyric acid receptor complex in brain. Brain Res. 404,355-360.
Majewska, M.D., Demirgoren, S., Spivak, C.E. and London, E.D. (1990) The neurosteroid dehydroepiandrosterone sulfate is an allosteric antagonist of the GABAA receptor. Brain-Res. 526,143-6
Majewska, M.D. and et al., (1986) Steroid hormone metabolites are barbiturate-like modulators of the GABA receptor. Science. 232,1004-7
Majewska, M.D., Mienville, J.M. and Vicini, S. (1988) Neurosteroid pregnenolone sulfate antagonizes electrophysiological responses to GABA in neurons. Neurosci-Lett. 90,279-84
Olsen R.W., and Sapp, D. W., 1995 Neuroactive steroid modulation of GABAA receptors Adv. Biochem. Psychopharmacol. 48, 57-74.
Park-Chung, M., Wu, F.S. and Farb, D.H. (1994) 3 alpha-Hydroxy-5 beta-pregnan-20-one sulfate: a negative modulator of the NMDA-induced current in cultured neurons. Mol-Pharmacol. 46,146-50
Park-Chung, M., and et al., (1997) Distinct sites for inverse modulation of N-methyl-D-aspartate receptors by sulfated steroids. Mol-Pharmacol. 52,1113-23
Paul, S. M. and Purdy, R. M. (1992) Neuroactive steroid. FASEB J. 6, 2311-2322.
Peters, J. A., Kirkness, E. F., Callachan, H., Lambert, J. J., and Turner, A. J., 1988 Modulation of the GABAA receptor by depressant barbiturates and pregnane steroids. Br. J. Pharmacol. 94,1257-1269.
Prince,R. J., and Simmonds, M. A. (1992) Steroid modulation of the strychnine-sensitive glycine receptor. Neuropharmocology 31,201-205.
Simmonds, M. A., and Turner, J. P., (1985) Antagonism of inhibitory amino acids by the steroid derivative RU5135. Br. J. Pharmacol. 84,631-635.
Wu-FS; Chen-SC; Tsai-JJ (1997) Competitive inhibition of the glycine-induced current by pregnenolone sulfate in cultured chick spinal cord neurons. Brain-Res.750,318-20
Wu-FS; Gibbs-TT; Farb-DH (1990) Inverse modulation of gamma-aminobutyric acid- and glycine-induced currents by progesterone. Mol-Pharmacol. 37(5): 597-602
Wu-FS; Gibbs-TT; Farb-DH (1991) Pregnenolone sulfate: a positive allosteric modulator at the N-methyl-D-aspartate receptor. Mol-Pharmacol. 40(3): 333-6
Wu-FS; Gibbs-TT; Farb-DH (1993) Dual activation of GABAA and glycine receptors by beta-alanine: inverse modulation by progesterone and 5 alpha-pregnan-3 alpha-ol-20-one. Eur-J-Pharmacol. 246(3): 239-46
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
系統版面圖檔 系統版面圖檔