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研究生:蘇家潁
研究生(外文):Chia Ying Su
論文名稱:微生物對毒性物質PCP生物分解性與膠凝沈降性之間的關係探討
論文名稱(外文):The Discussion of the Rate of Biodegradation and the Rate of Flocculation on the Toxicants -PCP- on the Microorganisms
指導教授:張有義張有義引用關係
指導教授(外文):Y. I. Chang
學位類別:碩士
校院名稱:東海大學
系所名稱:化學工程學系
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2001
畢業學年度:90
語文別:中文
論文頁數:171
中文關鍵詞:生物分解膠凝沈降五氯酚
外文關鍵詞:biodegradationbioflocculationPentachlorophenol
相關次數:
  • 被引用被引用:9
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本論文主要目的為探討當以Flavobacterium sp.(ATCC 39723)好氧批次式反應實驗來分解不同濃度的PCP時(五氯酚、50-150 ppm),在特殊鹽類培養基中添加不同的碳源,對此菌種生物分解性的影響。並藉由菌體粒徑和菌體表面電位的測定來分析菌體膠凝沈降性和生物分解性間的關係。
實驗結果發現,在純PCP中添加其他碳源可以減少Flavobacterium sp.生長的遲滯期,其中以Glutamate和glucose為碳源時,分解PCP的速度最快,而以Cellobiose為碳源時則是最慢,此和其他文獻(33)有相同的結果。在純PCP培養基中,菌體溶液的穩定度會有最小值出現;添加其他碳源的菌體溶液之穩定度則會隨著培養時間的增加而增加,且會隨著PCP濃度的增加而變小。
The main purpose of this thesis is to discuss the influences of the different carbon sources added in the special miner salts on the biodegradation of various concentration of PCP (Pentachlorophenol) by Flavobacterium sp.(ATCC 39723) in the aerobic batch system. The relationship between bioflocculation and biodegradation of Flavobacterium sp. is investigated by measuring Zeta potential and diameter of this bacterium.
Experimental results, demonstrated that the lag phase of Flavobacterium sp. can be shorten by adding different corbon sources. The degradation rate of PCP of this bacterium is the fastest when using glutamate and glucose as the carbon sources and is the slowest when using cellobiose. Stability ratio of the solution has the minimum value on the pure PCP media. The higher concentration of PCP, the better the flocculation behavior of this bacterium.
第一章 緒 論…………………………………………………………1
1-1 前 言…………………………………………………………1
第二章 文獻回顧………………………………………………………5
2-1 五氯酚的物化性質……………………………………………5
2-2 Flavobacterium sp. (ATCC 39723) 的簡介………………11
2-3 PCP的生物分解………………………………………………13
2-3-1 Flavobacterium sp.(ATCC39723)分解五氯酚之代謝機 構……………………………………………………………………………13
2-3-2 加入不同碳源對Flavobacterium sp.分解五氯酚影響的探討……………………………………………………………………………16
2-3-3 微生物膠體的粒徑變化………………………………………17
2-4 菌體粒徑、表面電位對其膠凝沈降的影響…………………18
2-4-1 膠體表面電荷的起源…………………………………………19
2-4-2 電雙層理論……………………………………………………20
2-4-3 DLVO 理論……………………………………………………25
2-4-4 膠體膠凝之動力學理論………………………………………28
2-5 生物分解之動力學模式………………………………………30
2-5-1 單細胞微生物的生長速率……………………………………30
2-5-2 基質分解速率與增殖收率……………………………………33
第三章 實驗設備與方法…………………………………………………34
3-1 實驗目的………………………………………………………34
3-2 實驗材料………………………………………………………35
3-2-1 實驗藥品………………………………………………………35
3-2-2 實驗儀器與設備………………………………………………36
3-2-3 菌種……………………………………………………………37
3-3 實驗方法………………………………………………………38
3-3-1 菌體的培養……………………………………………………38
3-3-2 五氯酚溶液的製備……………………………………………42
3-4 實驗分析………………………………………………………43
3-4-1 生物分解實驗…………………………………………………43
3-4-2 表面電位的測定………………………………………………44
3-4-3 菌體粒徑大小的測定…………………………………………44
3-4-4 沈降實驗………………………………………………………45
3-5 檢量線…………………………………………………………46
3-5-1 PCP濃度的檢量線……………………………………………46
3-5-2 濁度值對細胞個數的檢量線…………………………………47
3-6 儀器操作與設定………………………………………………48
3-6-1 UV-VIS光譜儀…………………………………………………48
3-6-2 表面電位測定儀………………………………………………50
3-6-3 庫特計數器……………………………………………………51
第四章 結果與討論………………………………………………………52
4-1 固態和液態接種培養的Flavobacterium sp. ………………52
4-2 檢量線……………………………………………………………52
4-2-1 PCP濃度的檢量線………………………………………………52
4-2-2 濁度值對Flavobacterium sp. 細菌濃度個數的檢量線……55
4-2-3 Flavobacterium sp. 細胞乾重對濁度值的檢量線…………57
4-3 Flavobacterium sp.細菌生長曲線……………………………59
4-3-1 OD560和細菌個數的關係………………………………………59
4-3-2 加入其他碳源時之Flavobacterium sp.生長曲線比較………61
4-3-3 不同PCP濃度的影響……………………………………………67
4-3-4 相同PCP濃度不同碳源時的比較………………………………67
4-4 生長動力學模式…………………………………………………73
4-5 基質分解速率與產率……………………………………………78
4-6 Flavobacterium sp.菌體的粒徑變化…………………………83
4-7 表面電位的測定…………………………………………………88
4-8 沈降實驗之穩定度值……………………………………………94
第五章 結論………………………………………………………………105
5-1 結 論……………………………………………………………105
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