臺灣博碩士論文加值系統

本研究探討藿香(植物基源為廣藿香Pogostemon chablin)之抗氧化成分，與其揮發性成分，主要分為以下兩個項目：
1.廣藿香之抗氧化成分
廣藿香粉末依序以正己烷、乙酸乙酯及甲醇萃取、過濾、濃縮後檢測其抗氧化性，三種溶劑萃取物之抗氧化性依序為BHA＞甲醇萃取物＞α­生育醇＞乙酸乙酯萃取物＞正己烷萃取物＞控制組。選擇具抗氧化較佳之廣藿香甲醇萃取物，經XAD-7液相層析區分出顯現強抗氧化性的沖提部分，利用高效液相層析法分析，選擇具抗氧化性，且所含成分較不複雜之區分，重複以高效液相層析分離純化，藉各種光譜分析法鑑定，得到三種純化物質，分別為adenosine、4-hydroxyethyl-catachol-1-O-(2’-α-L-rhamnopyranosyl)-β- D- gluco- pyranoside及ο-coumaroyl-β- D- glucopyranoside。
2.廣藿香之揮發性成分
廣藿香粉末利用水蒸氣蒸餾-溶劑萃取法及超臨界二氧化碳萃取法萃取揮發性成分，並分析其化學組成及檢測揮發性成分與廣藿香不同溶劑萃取物之抗氧化性。結果顯示，廣藿香揮發性萃取物經GC及GC-MS分離鑑定，共鑑定出19種化合物，其中醇類6種，烯類10種，酯類1種、酸類1種及1種酚型化合物。各種萃取物抗氧化性利用硫氰酸鐵法檢測，其強弱依序為BHA＞甲醇萃取物＞α-生育醇＞乙酸乙酯萃取物＞ 正己烷萃取物＞超臨界二氧化碳萃取物＞水蒸氣蒸餾-溶劑萃取物＞控制組。

The purposes of this study were to investigate antioxidative and volatile components from Pogostemon chablin. The results were summarized as the following:
(1) The antioxidative components of Pogostemon chablin
The ground powder of Pogostemon chablin was extracted with n-hexane, ethyl acetate and methanol. The antioxidative activity of the extract was measured by the ferric thiocyanate method. The order of antioxidative activity was : BHA > methanol extract > α-tocopherol > ethyl acetate extract > n-hexane > control. Due to the strong antioxidative activity of methanol extract, it was fractionated and isolated by various chromatographies to obtain three pure components— adenosine、4-hydroxyethyl- catachol-1-O- (2’-α- L- rhamnopyranosyl)-β- D- glucopyranoside及ο- coumaroyl-β- D- glucopyranoside.
(2) Volatile components of Pogostemon chablin
The volatile extract was obtained from Pogostemon chablin by Likens-Nickerson extraction and supercritical carbon dioxide extraction. It was isolated and identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The 19 components of the volatile extract had been identified, including 6 alcohols, 10 terpenes, 1 ester, 1 acid and 1 phenolic compound . The antioxidative activity of the volatile extract and the various solvent extract was analyzed by the ferric thiocyanate method. The order of antioxidative activity was: BHA > methanol extract >α-tocopherol > volatile extracts > ethyl acetate extract > n-hexane > supercritical carbon dioxide extract > Likens-Nickerson extraction > control

中文摘要 ----------------------------------------------------- Ⅱ
英文摘要 ----------------------------------------------------- Ⅲ
圖目錄 ----------------------------------------------------- Ⅳ
表目錄 ----------------------------------------------------- Ⅶ
第一章 前言----------------------------------------------- 1
第二章 抗氧化分之研究-------------------------------- 4
第一節 文獻整理----------------------------------------- 4
第二節 抗氧化成分之研究----------------------------- 45
材料介紹----------------------------------------- 45
材料與方法-------------------------------------- 46
結果與討論-------------------------------------- 52
第三章 揮發性成分之研究----------------------------- 91
第一節 文獻整理----------------------------------------- 91
第二節 揮發性成分之研究----------------------------- 105
材料與方法-------------------------------------- 105
結果與討論-------------------------------------- 110
結論----------------------------------------------- 116
參考文獻----------------------------------------- 117

八木國夫和五島雄一郎（1981）過氧化脂質與疾病。醫學書院，東京。
五十嵐脩、金田尚志、福場博保、美濃真（1986）過氧化脂質與營養。光生館，東京。
中谷延二 (1989) 香辛料的抗氧化性、抗菌性。香辛料成分的食品機能，岩井和夫、中谷延二編，p.69-96。光生館。
內山充、松尾光芳、嵯峨井勝（1985）過氧化脂質與生物體。學會出版中心，東京。
孔祥琳（1991）羌活揮發性、藥理活性及抗氧化性成分之研究。靜宜大學食品營養研究所碩士論文。
江蘇新醫學阮（1986）中藥大辭典（上，下）。上海科學技術出版社，上海。
吳芳真（1995）草果抗氧化成分之研究。東海大學食品科學研究所碩士論文。
吳芳真，蘇正德（1996）草果抗氧化成分之分離，純化及構造鑑定。中國農業化學會誌 3:438-451。
吳怡蓉（1998）高良薑揮發性及抗氧化成分之研究。東海大學食品科學研究所碩士論文。
宋禮邦（1994）茶梗抗氧化成分之研究。東海大學食品科學研究所碩士論文。
岩井和夫、中谷延二（1989）香辛料成分的食品機能。光生館，東京。
金田尚志，石井清之助（1953）多元不飽和脂肪酸之營養價值與毒性。日水誌 19:171-177。
金田尚志，渡邊寬子（1963）加工食品中油脂之過氧化物量。營養與食糧 16:211-212。
林天送（1995）你的生命活力-從自由基談起。健康世界雜誌社，台北。
林書玉（1997）紅豆蔻揮發性及抗氧化成分之研究。東海大學食品科學研究所碩士論文。
洪千雅（1994）香薷及槐花抗氧化成分之研究。東海大學食品科學研究所碩士論文。
洪裕閩（2001）雷丸抗氧化、螢光成分及揮發性成分之研究。東海大學食品科學研究所碩士論文。
張明慧，蘇正德和吳天賞（1996）茵陳蒿抗氧化成分之研究。食品科學23:594-607。
張為憲等（1995）食品化學。華香園出版社，台北。
張毅偉、蘇正德（1998）益智仁主要揮發性成分及其抗氧化性之研究，東海學報38:93-105。
陳振坤（1992）二氧化碳萃取精油之十年歷程。香料資訊4：21-33。
陳惠英（1996）茶葉萃取物抗致突變性及抗氧化特性之研究。中興大學食品科學研究所博士論文。
陳潔如（2001） 含類黃酮蔬菜抗氧化性及青花菜抗氧化成分之研究。東海大學食品科學研究所碩士論文。
喬長誠（1986）香味研究方法之簡介。食品工業18：22-27。
楊宗熙（1996）油脂氧化安定性與天然抗氧化劑效力之評估。食品工業月刊23-33。
葉佳聖和蘇正德（1993）芡實抗氧化成分之研究。東海學報34：1115- 1131。
葉佳聖和蘇正德（1993）補骨脂抗氧化成分之研究。食品科學20：574-585。
滿田久輝、安本教傳、岩見公和（1967）抗氧化成分對亞麻油酸之自氧化的影響。營養與糧食19：210-214。
蔡文鈐（2001）細辛揮發性及抗氧化成分之研究。東海大學食品科學研究所碩士論文。
樊謙騰（1996）鳳凰花及山竹果殼抗氧化成分與花青素之研究。東海大學食品科學研究所碩士論文。
劉珊珊（1996）肉豆蔻揮發性及抗氧化成分之研究。東海大學食品科學研究所碩士論文。
謝明訓和邱年永（1993）藿香與廣藿香考述。明通醫藥 200：16-21。
簡永亮（2000）沙苑子、馬櫻丹花抗氧化成分及沙苑子揮發性成分之研究。東海大學食品科學研究所碩士論文。
顏國欽（1993）食品安全學。藝軒出版社，台北市。
蘇正德和陳雅瑛（1991）省產稻米外殼抗氧化性之研究調查。東海學報 32：719-730。
蘇正德、蔡文騰、張基煌、蘇女淳（1991）茶湯與茶渣兒茶酚含量及抗氧化性之調查研究。食品科學18：234-248。
難波恒雄（1980）原色和漢藥圖鑑（上，下）。保育社，大阪。
Allen, J.C. and Hamilton, R. T.（1983）Rancidity in Foods, Applied Science Publishers, New York.
Aruoma , O. I. (1994) Nutrition and health aspects of free radicals and
antioxidants. Food Chem. Toxic. 32:671-683.
Bawn, C. E. H.(1953) Free-radical reactions in solution initiated by heavy metal ions. Disc. Farad. Soc. 14:181-199.
Branen, A. C.（1975）Toxicologes and biochemistry of butylated hydroxy anisol and butylated hydroxy toluene, J. Am.Oil Chem. Soc. 53：59.
Charalambous, G. (1987) Analysis of Foods and Beverages. Academic Press Inc.,New York
Cu, J.-Q., Perieau, F., Delmas, M.and Gaset, A.(1989) Comparison of thechemical composition of carrot seed essential oil extracted by different solvents. Flavour and Fragrance J 4:225-231 .
Cuvelier, M.E., Berset, C. and Richard, H.（1994）Antioxidant constituents in sage（Salvia officinalis L.）. J. Agric. Food Chem. 42： 665-669.
Eiserle, R. J. and Rogers, J, A. (1972) The composition of volatile oils derived from oleoresins. J. Am. Oil Chem. Soc. 49: 573- 577.
Farmer, E. H., Bloomfield, G. F., Sundralingam, A. and Sutton, D. A. (1942) The course and mechanism of autoxidation reactions in olefinic and polyolefinic substances, including rubber. Trans Faraday Soc. 38:348-356.
Fennema, O.R. (1985) Food Chemistry, edition 2, Marcel Dekker, Inc., New York .
Frankel E. N.（1984）Lipid oxidation：Mechanisms, products and biological significance. J. Am. Oil Chem. Soc. 61：1908-1917.
Fukada, Y., Nagata, M., Osawa, T., and Namiki, M.（1986）Contribution of lignan analogues to antioxidative activity of refined unroasted sesame seed oil. J. Am. Oil Chem. Soc. 63：1027-1031.
Fukuda,Y. and Namiki, M. (1988) Recent studies on sesame seed and oil, Nippon Shokuhin Kogyo Gakkaishi 35:552-562.
Halliwell, B. and Gutteridge John M. C.（1984）Oxygentoxicity, oxygenradicals, transition metals and disease. Biochem. J. 219：1-14.
Hara, T. and Kubota, E. (1984) Changes in aroma components of green tea during the fring process. Nippon Nogeikagaku Kaishi 58:25-30.
Heath, H. B. (1978) Flavor Technology. AVI Publishing Co., Inc.,Wesport, CT.
Heath, H. B. (1981) Source Book of Flavors. AVI Publishing Co., Inc., Westport,CT
Hirosue, T., Matsuzawa, M., Irie, I., Kawai, H. and Hosogai, Y. （1988） On the antioxidative activities of herbs and spices. Nippon Shokuhin Kogyo Gakkaishi 35：630-633.
Hirosue, T., Kawai, H. and Hosogai, Y.（1978）On the antioxidative activities of crude drugs. Nippon Shokuhin Kogyo Gakkaishi 25：691-694.
Houlihan, C.M. Ho C.T. and Chang,S.S.,（1984）Elucidation of the chemical structure of a novel antioxidant, rosemaridi-phenol; isolated from rosemary. J. Am. Oil Chem. Soc. 61：1036-1039.
Houlihan, C. M., Ho, C. T., and Chang, S. S.（1985）The structures of rosemariquinone a new antioxidant isolated from Rosemarinu officinalis L. J. Am. Oil Chem. Soc. 62：96-98.
Hudson, B. J. F.,（1990）Food antioxidants, Elsevierscience publishing Co. New York.
Igarashi, K., Takanashi, K., Makino, M. and Yasui, T.（1989）Antioxidative activity of major anthocyanin isolated from wild grapes (Vitis coignetiae). Nippon Shokuhin Kogyo Gakkaishi 36：852-856.
Igarashi, K. Yoshida, T. and Suzuki, E (1993) Antioxidative activity of nasunin in Chouja-nasu (little eggplant, Solanum Melongena L. Chouja’). Nippon shokuhin Kogyo Gakkaishi 40:138-143.
Inatani, R., Nakatani, N. and Seto, H.（1982）Structure of a new antioxidative phenolic diterpene isolated from rosemary（Rosmarinus officinalis L., Agric. Biol. Chem. 46：1661-1666.
Inatani, R., Nakatani, N. and Fuwa, H.（1983）Antioxidative effect of the constituents of rosemary（Rosmarinus officinalis L.）and their derivatives. Agric. Biol. Chem. 47：521-528.
Ito, N., Hirosye, M., Fukuashima, S., Tsuda, H., Shiria, T. and Tatematsu, M.（1986）Studies on antioxidant：their carcinogenic and modifying effects on chemical carcinogenesis. Food Chem. Toxic. 24：1071-1082
Kellogg, E. W.Ⅲ and Fridovich, Ⅰ.(1975) Superoxide, hydrogen peroxide and singlet oxygen in lipid peroxidation by a xanthine oxidase system. J. Biol. Chem. 250:8812-8815.
Kikuzaki, H. and Nakatani, N. (1987) A new antioxidative glucoside isolated from oregano (OreganumVulgare L.). Agric. Biol. Chem. 51:2727-2731.
Kubow, S.,（1990）Toxicity of dietary lipid peroxidation products. Trends in Food Science & Technology. 1：67-70.
Larson, R. A. (1988) The antioxidants of higher plants. Phytochem. 27:123-126.
Lee, M.-H.（1988）Natural antioxidant from teas, in〝Proceedings of the international symposium on recent development in tea of production〞p.299. Taiwan Tea Experiment Station,Taoyuan.
Loft, S. Vistisen, K. Ewertz, Z. Tjonneland A. Overvad, K. and Poulsen,H. E. (1992) Oxidative DNA damage estimated by 8- hydroxyquanosine excretion in humans : influence of smoking, gender and body mass index. Carcinogenesis 13:2441-2447.
Lu, Y. and Foo, L. Y. (2000) Antioxidant and radical scavenging activities of polyphenols from apple pomance. Food Chemistry, 68：81-85.
Matsuzaki, T. and Hara. Y.（1985）Antioxidative activity of tea leaf catechin. Nippon Nogei Kaguku Kaishi 59：129-134.
Marrse, H. and Belz, R. (1981) Isolation, separation and identification of volatiles compound in aroma . p.21, Kluwer Boston Inc., Hingham, H. A. U.S.A.
Nakatani, N and Kikuzaki, H.（1989）Structure of a new antioxidative phenolic acid from oregano（Oreganum vulgare L.）. Angric. Biol. Chem. 53：519-524.
Nakatani, N and Kikuzaki, H.（1993）Antioxidant effects of some ginger constituents, J. of Food Science 58:1407-1410.
Nakatani, N., Miura, K., and Inagaki, T.（1989）Structure of new deodorant biphenyl compounds from thyme （Thymus Vulgaris L.） and their activity against methylmercaptan. Agric. Biol. Chem. 53：1375-1381.
Namiki, M. (1990) Antioxidants / antimutagens in Foods. Crit. Rev. Food Sci. Nutri. 29:281-300.
Nawar, W. W. (1985) Lipid in 〝Food Chemistry〞, p. 139-244. Fennema, O. R. Ed., Marcel Dekker, INC., New York.
Niki, E., Yamamoto, Y., Komuro, E. and Sato, K. （1991）Membrane damage due to lipid oxidation. Am. J. Clin. Nutr.53：201-205.
Osawa, T., Nagata, M. (1985), A novel of antioxidants isolated from leaf waxes of Eucalyptus leaves. J. Agric. Food Chem. 33：777-780.
Pratt, D.E. and Miller, E.E. (1984). A flavonoid antioxidant in spanish peanuts (Arachia hypogoea). J. Am. Oil Chem. Soc. 61：1064-1067.
Perkins, E. G. (1967) Formation of nonvolatile decomposition products in heated fats and oils. Food Technol. 21:125-134.
Pesek, C.A., Wilson, L.A. and Hammond, E.G. (1985) Spice quality: effect of cryogenic and ambient grinding on volatiles. J . Food Science 50:599-601
Pruthi, J. S. (1980) Spices and comdiments : chemistry , microbiology, technology. Advances in Food Research Supp. 4. Academic press , Inc. , New York.
Ramarathnam, N., Osawa, T., Namiki, T. and Kawakishi, S. (1988) Chemical studies on novel rice hull antioxidants. I. Isolation, Fractionation and partial characterization. J. Agric. Food Chem. 36: 732-737.
Ramarathnam, N., Osawa, T., Namiki, T. and Kawakishi, S. (1989) Chemical studies of novel rice antioxidants. Ⅱ. Identification of isovitexin, a C-glycosyl flavonoid. J. Agric. Food Chem. 37: 316- 319.
Sherwin, E. R.（1978） Oxidation and antioxidants in fat and oil processing. J. Am. Oil Chem. Soc. 55：809-814.
Su, J.-D., Osawa, T., and Namiki, M.（1986）Screening for antioxidative activity of crude drugs. Agric. Biol. Chem. 50:199-203.
Su, J.-D., Osawa, T., and Namiki, M.（1987a）. Antioxidative flavonoids isolated from Osbeckia chinensis L. Agric. Biol.Chem. 51：2801- 2803.
Su, J.-D., Osawa, T., Kawakishi, S. and Namiki, M. (1987b). A novel antioxidative synergist isolated from Osbeckia chinenesis L. Agric. Biol. Chem.51：3449-3450.
Su, J.-D., Osawa T., Kawakishi, S. and Namiki , M. (1988) Tannin antioxidants from Osbeckia chinensis L..Phytochemistry. 27: 1315-1319.
Su, J.-D. (1992). Investigation of antioxidative activity and tocopherol contents on Chinese crude drugs of fruits of seeds. Food Science 19： 12-24.
Thomson, D. M. (1986) The meaning of flavour. Academic Press, Inc., New York.
Toda, S., Miyase, T., Arichi, H., Tanizawa, H., and Takino, Y.（1985）Natural antioxidants II. Antioxidative compounds isolated from seeds of Plantogo asiatica Linne. Chem. Pharm. Bull. 33：1725-1728.
Waters, W.A. (1971) The kinetics and mechanism of metal-catalysed autoxidation. J.Am. Oil Chem. Soc. 48:427-433.
Westwood, S. A.（1993）Supercritical fluid extraction and its use in chromatographic sample preparation . Balckie, Glasgow.
Wiliam, A. R. and Thomson, M. D. (1978) Medicine s from the earth. Mc Graw Book Company,New York.
Yagi, K. (1987) Lipid peroxides and human disease. Chem. Phys. Lipids. 45:337-341.