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研究生:蘇淑琍
論文名稱:薑黃素抑制v-Src癌化細胞的生長及移動
論文名稱(外文):Curcumin inhibits v-Src transformed cells mitogenesis and migration
指導教授:馬明琪
學位類別:碩士
校院名稱:中山醫學大學
系所名稱:生物化學研究所
學門:生命科學學門
學類:生物化學學類
論文種類:學術論文
論文出版年:2003
畢業學年度:91
語文別:中文
論文頁數:62
中文關鍵詞:薑黃素SrcFAK
相關次數:
  • 被引用被引用:1
  • 點閱點閱:317
  • 評分評分:
  • 下載下載:83
  • 收藏至我的研究室書目清單書目收藏:3
薑黃素 (curcumin) 是一個歷史悠久廣為人知的天然食用色素,它具有抗發炎、抗氧化、以及抗癌的效果。在本文研究中,我們發現薑黃素可以抑制 v-Src 的活性,導致 Src 受質 Shc、 cortactin、 FAK 酪氨酸磷酸化減少。而在我們 in vitro kinase 的實驗裡,我們也証明了薑黃素可直接抑制 Src 的活性。由於Src活性的下降,使Shc的 Pi-Tyr-317 減少,進而降低ERK 的活性,抑制 v-Src 癌化細胞的生長。此外,薑黃素也可直接及間接地抑制 FAK 的活性。藉由降低cortactin 的酪氨酸磷酸化及 FAK 酵素活性,薑黃素更減緩了 v-Src 癌化細胞的移動 (migration)。據我們的所知,這是第一篇有關薑黃素藉由抑制 Src 及 FAK 的酵素活性,進而抑制細胞生長及移動的報導。
Curcumin (diferulonylmethane) is a well-known agent with anti-inflammatory, antioxidant, and anticarcinogenic properties. In this study, we observed that curcumin inhibited the kinase activity of v-Src, which led to a decrease in tyrosyl substrate phosphorylation of Shc, cortactin, and FAK. Our in vitro kinase experiment revealed that the inhibitory effect of curcumin on Src could be direct. Consistent with the abrogation of Src activity was the reduction of Src Tyr-416 phosphorylation, Src-mediated Shc Tyr-317 phosphorylation, decreased ERK activation, and cell proliferation in v-Src transformed cells. Remarkably, curcumin not only exerted its negative effect on FAK via the disappearance of Src-mediated FAK phosphorylation, but also directly inhibited its enzymatic activity. Concurrent to reduced cortactin tyrosyl phosphorylation and FAK kinase activity was the abolishment of v-Src-mediated cell mobility. To our knowledge, this is the first report indicating that curcumin can retard cellular growth and migration via downregulation of Src and FAK kinase activity.
頁數
中文摘要 3
英文摘要 4
縮寫檢索表 5
第一章 序論 7
第二章 實驗材料與方法 14
第三章 結果 31
第四章 討論 38
參考文獻 42
圖表 49
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