臺灣博碩士論文加值系統

本論文主要目的在研究馬鞭草科(Verbenaceae)黃荊屬(Vitex)中黃荊(Vitex negundo)的化學成分及抗疱疹活性之研究。由黃荊的氯仿層獲得一個新的化合物6-Hydroxy vitexilactone (1)，與一個已知的化合物，vitexilactone (2)；化合物1-2均是屬於labdane-type diterpene。從乙酸乙酯層獲得一個已知的芳香族有機酸，p-hydroxybenzoic acid (3)。上述化合物之化學結構均是經由各類型的圖譜(1H和13C NMR、HMQC、1H-1H COSY、HMBC、NOESY及IR)解析與物理資料(熔點、旋光度)的測定及比對相關文獻資料而建立。利用西方墨漬分析法(western blot analysis)進行抗疱疹活性之測試，化合物1-3無顯著的活性。

The main objectives of this research are to determine the chemical constituents and their activities to anti-Herpes virus of the methanolic extracts of Vitex negundo ,Verbenaceae. Two compounds have been obtained from the chloroform partitioned layer, including a new compound 6-hydroxy vitexilactone (1), and one known compound, vitexilactone (2). Both of them are belonged to labdane-type diterpene. One aromatic organic acid, p-hydroxybenzoic acid (3), had been obtained from the ethyl acetate partitioned layer. The structure of above compounds were determined on the basis of extensive NMR experiments, including HMQC, HMBC, 1H-1H COSY and NOESY and their physical properties (such as melting point, optical rotation) and the comparison with related references. The activity of anti-Herpes virus was analyzed by bio-activity screen method. Compounds 1-3 were not showed the significant activity.

目錄……………………………………………………………………I
圖表目錄………………………………………………………………II
中文摘要………………………………………………………………1
英文摘要………………………………………………………………2
第一章 緒論…………………………………………………………3
第一節 前言………………………………………………………3
第二節 文獻回顧…………………………………………………8
第三節 植物之分佈及型態………………………………………44
第四節 研究目的…………………………………………………45
第二章實驗程序與方法………………………………………………46
第一節 研究材料成分之抽取與分離……………………………46
第二節 生物活性之篩檢…………………………………………51
第三節 使用儀器與材料…………………………………………54
第三章實驗結果與結構解析…………………………………………56
第一節 6-Hydroxy vitexilactone (1)……………………………56
第二節 Vitexilactone (2)…………………………………………64
第三節 p-Hydroxybenzoic acid (3) ……………………………72
第四節 生物活性…………………………………………………78
第四章 結論…………………………………………………………84
第五章 實驗數據整理………………………………………………85
第六章 參考文獻……………………………………………………87
圖表目錄
頁次
圖1 黃荊之分離流程………………………………………………48
圖2 氯仿層之分離流程……………………………………………49
圖3　乙酸乙酯層之分離流程………………………………………50
圖1-1 6-Hydroxy vitexilactone (1)之1H NMR光譜(CDCl3) ………………………………………………………59
圖1-2 6-Hydroxy vitexilactone (1)之13C NMR光譜 (CDCl3) ………………………………………………………59
圖1-3 6-Hydroxy vitexilactone (1)之COSY光譜 (CDCl3) ………………………………………………………60
圖1-4 6-Hydroxy vitexilactone (1)之HMBC光譜 (CDCl3) ………………………………………………………61
圖1-5 6-Hydroxy vitexilactone (1)之HSQC光譜 (CDCl3) ………………………………………………………61
圖1-6 6-Hydroxy vitexilactone (1)之NOESY光譜 (CDCl3) ………………………………………………………62
圖1-7 Selective 1H-1H COSY and HMBC correlation of 1 ……63
圖1-8 Selective NOE correlation of 1 …………………………63
圖2-1 Vitexilactone (2)之1H NMR光譜 (CDCl3) ……………67
圖2-2 Vitexilactone (2)之13C NMR光譜 (CDCl3) ……………68
圖2-3 Vitexilactone (2)之DEPT光譜 (CDCl3) ………………68
圖2-4 Vitexilactone (2)之COSY光譜 (CDCl3) ………………69
圖2-5 Vitexilactone (2)之HSQC光譜 (CDCl3) ………………69
圖2-6 Vitexilactone (2)之HMBC光譜 (CDCl3) ………………70
圖2-7 Vitexilactone (2)之NOESY光譜 (CDCl3)………………70
圖2-8 Selective 1H-1H COSY and HMBC correlation of Vitexilactone (2) ……………………………………………………………………71
圖2-9 Selective NOE correlation of Vitexilactone (2) ……………………………………………………………………71
圖3-1 p-Hydroxybenzoic acid (3)之1H NMR光譜(CD3COCD3)…73
圖3-2 p-Hydroxybenzoic acid (3)之13C NMR光譜(CD3COCD3)…74
圖3-3 p-Hydroxybenzoic acid (3)之DEPT光譜(CD3COCD3)……74
圖3-4 p-Hydroxybenzoic acid (3)之HSQC光譜(CD3COCD3)……75
圖3-5 p-Hydroxybenzoic acid (3)之COSY光譜(CD3COCD3)……76
圖3-6 p-Hydroxybenzoic acid (3)之HMBC光譜(CD3COCD3)……77

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