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研究生:蔡銘偉
研究生(外文):Ming-Wei Tsai
論文名稱:乳羊在分娩後四個月內之乳過氧化酶系統、體細胞數及乳量之變化
論文名稱(外文):The Profiles of Lactoperoxidase System, Somatic Cell Counts and Milk Yields during Four Months of Postparturition in Does
指導教授:白火城白火城引用關係
指導教授(外文):Huo-Cheng Peh
學位類別:碩士
校院名稱:國立中興大學
系所名稱:畜產學系
學門:農業科學學門
學類:畜牧學類
論文種類:學術論文
論文出版年:2003
畢業學年度:91
語文別:中文
論文頁數:78
中文關鍵詞:羊乳乳過氧化酶硫氰酸鹽過氧化氫體細胞數乳量生菌數
外文關鍵詞:goat milkLactoperoxidasethiocyanatehydrogen peroxidesomatic cell countsmilk yieldsstandard plate counts
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本試驗之目的,為探討母山羊在泌乳期間之乳過氧化酶系統、體細胞數與乳量的變化。本試驗共使用母山羊8頭,自母山羊分娩後至泌乳期之前4個月,每週分別記錄個別母山羊之乳量,並採集上午擠乳時的分房乳樣,以分析乳樣的乳過氧化酶活性、硫氰酸鹽與過氧化氫含量,並測定其生菌數及體細胞數。試驗結果顯示: (1) 乳過氧化酶活性:羊乳樣品在採集時之平均乳過氧化酶活性為1.390±0.021 U/ml,而採集之羊乳樣品經貯藏3天後,其平均乳過氧化酶活性降為1.216±0.020 U/ml,且與採樣時之乳過氧化酶活性呈顯著差異 (P<0.05) 。初產與經產母山羊之羊乳中乳過氧化酶活性活並無顯著差異 (P>0.05) 。母山羊在泌乳第一週之乳過氧化酶活性顯著較第二週以後之其他週次為低 (P<0.05) ,而且隨著泌乳週次之增加乳過氧化酶之活性逐漸升高,至泌乳第12週時,乳過氧化酶活性達高峰,其後乳過氧化酶活性則呈逐漸降低的趨勢。 (2) 硫氰酸鹽:羊乳樣品在採集時之平均硫氰酸鹽含量為0.082±0.001 mM,而採集之羊乳樣品經貯藏3天後,其平均硫氰酸鹽含量降為0.075±0.001 mM,且與採樣時之硫氰酸鹽含量呈顯著差異 (P<0.05) 。初產與經產母山羊之羊乳硫氰酸鹽含量,並無顯著差異 (P>0.05) 。母羊在泌乳第一週之羊乳硫氰酸鹽含量為0.106 mM,且顯著較其他週次為高(P<0.05),而其他週次之羊乳硫氰酸鹽含量間並無顯著差異 (P>0.05) 。 (3) 過氧化氫: 羊乳樣品在採集時之平均過氧化氫含量為0.246±0.004 mM,而採集之羊乳樣品經貯藏3天後,其平均過氧化氫含量降為0.207±0.004 mM,並與採樣時羊乳中之過氧化氫含量呈顯著差異 (P<0.05) 。初產與經產母山羊之羊乳過氧化氫含量,並無顯著差異 (P>0.05) ;而在泌乳期間羊乳之過氧化氫含量呈現不規則之變化。(4) 生菌數:本試驗測得採樣時羊乳之平均生菌數為4.568±0.023 log CFU/ml,而經密閉貯存在4℃下,經3天後其生菌數增加為4.746±0.018 log CFU/ml,且兩者呈顯著差異 (P<0.05)。初產與經產母山羊之羊乳生菌數,並無顯著差異 (P>0.05) 。在試驗期間,不同泌乳週次之羊乳生菌數間,並無顯著差異 (P>0.05) ,且無明顯之規律性變化趨勢。 (5) 體細胞數:本試驗乳樣之平均羊乳體細胞數為482.3×103 cell/ml。初產母山羊之平均羊乳體細胞數為373.33±40.31×103 cell/ml,而經產母山羊之羊乳體細胞數為591.30±356.96×103 cell/ml,其中以經產母山羊之羊乳體細胞數顯著較初產母山羊為高 (P<0.05)?。母山羊在泌乳第1週時,羊乳之體細胞數最高,為1075.7×103 cell/ml,而後隨著泌乳時間之增加,羊乳體細胞數逐漸下降,但至泌乳第12週後,羊乳體細胞數則又逐漸上升。(6)? 乳量:試驗期間泌乳母山羊之每日平均泌乳量為2.130±0.071㎏,其中以經產母山羊之每日平均乳量顯著較初產母山羊為高? (P<0.05)? 。在泌乳之前3週,乳量隨著泌乳週次之增加而逐漸上升,至第3週達到泌乳高峰,而此高峰持續至第9週,其後乳量則隨泌乳週次之增加而逐漸下降。
The purpose of this study was to examine the change of Lactoperoxidase system, somatic cell counts, and milk yields in lactating does from kidding to four months of postparturient periods. Eight does were used in this experiment. During the experimental period, milk yields were recorded weekly, and lactoperoxidase activities (LP), thiocyanate (SCN-) contents, hydrogen peroxide (H2O2) contents, somatic cell counts (SCC) and standard plate counts (SPC) of milk samples were analyzed weekly. The results showed that (1) LP: The average LP activitity of milks on sampling day were 1.390±0.021 U/ml, and it dropped to 1.216±0.020 U/ml after storage for 3 days. LP activities of milk on sampling day was significantly higher than those of storage for 3 days. Parity didn’t affect the activities of lactoperoxidase. Lactoperoxidase activities of milk samples at first lactating week were significantly lower than those of the other weeks. (2) SCN-: The average SCN- contents of milks on sampling day were 0.082±0.001 mM, and it dropped to 0.075±0.001 mM after storage for 3 days. SCN- contents of milks on sampling day were significantly higher than those of storage for 3 days. Parity didn’t affect the SCN- contents of milks. SCN- contents of milk samples at first lactating week were higher than those of the other weeks. (3) H2O2: The average H2O2 contents of milks on sampling day were 0.246±0.004 mM, and it dropped to 0.207±0.004 mM after storage for 3 days. H2O2 contents of milks at sampling day were significantly higher than those of storage for 3 days. Parity didn’t effect the H2O2 contents of milks. During the lactating period, the H2O2 contents of milks changed irregularly. (4) SCC: The average SCC of milks were 482.3×103, and it increased with parity number. Milk SCC at first lactating week was higher than those of the other weeks. From 2nd to 11th weeks of lactating period, milk SCC decreased gradually. (5) Milk yields: The average milk yields of lactating does were 2.130±0.071 kg per day. Milk yields of lactating does were significantly increased with parity. Milk yields at 3rd to 9th weeks of lactating period were higher than those of the other weeks. (6) SPC: The average SPC of milks at sampling day were 4.568±0.023 log CFU/ml, and it increased to 4.746±0.018 log CFU/ml after storage for 3 days. SPC of milk at sampling days was significantly lower than those of storage for 3 days. Parity didn’t affect the SPC of milks. During the lactating period, SPC of milks changed irregularly.
壹、中文摘要 1
貳、前言 3
參、文獻檢討 4
一、 台灣地區乳用山羊簡介 4
二、 泌乳週期中乳腺之生長 4
三、 乳過氧化系統之發現 6
四、 LP system之作用機制 9
五、 乳過氧化系統之應用 11
六、 乳過氧化系統中三種組成分之分述 14
七、 乳中的體細胞數 23
八、 乳量 27
肆、試驗材料與方法 31
一、 試驗動物 31
二、 樣品之採集及處理 31
三、 分析項目及方法 31
四、 結果之統計分析 39
伍、結果與討論 40
一、 母羊在試驗期間之各項測定值比較 40
二、 各項數值間之相關 59
陸、結論 62
柒、參考文獻 64
捌、英文摘要
中國國家標準。1991。食品微生物之檢驗法-生菌數之檢驗,CNS 總號 10890,類號N 6186。經濟部中央標準局,台北市。
白火城。1996。本省乳用山羊事業之沿革與未來展望。興大農業 17:16-24。
白火城。2002。家畜解剖生理學實習。國立中興大學,台中市。
行政院農業委員會。2001。農業統計年報,pp.146-166。台北市。
林炯仁。2002。台灣養羊事業概況。羊協一家親 23:17-21。
林春基。1990。羊乳體細胞數與乳質關係之研究。碩士論文,國立中興大學,台中市。
林春基、張勝善。1994。羊乳體細胞數與乳質關係之研究。中畜會誌23:407-418。
林冠彰。1998。生牛乳之乳過氧化酵素系統及其延長貯存之研究。碩士論文,私立東海大學,台中市。
徐銘辰,2002。泌乳山羊血中及乳中嗜中性白血球經不同熱處理方式其呼吸暴發及凋亡之差異。碩士論文,國立中興大學,台中市。
張勝善。1995。牛乳與乳製品。增訂再版。長河出版社。台北市。
劉姿錡。2001。胎次與泌乳期對羊乳品質之影響。碩士論文,國立中興大學,台中市。
楊正彬。2000。乳山羊乳房炎檢測方法之研究。碩士論文,國立台灣大學,台北市。
鄭三寶、白火城、朱志成、劉炳燦。1997。畜牧(二)。東大圖書公司,台北市。
蔡濱燦。2000。羊乳組成分與含氮化合物與體細胞數之關係。碩士論文,私立東海大學,台中市。
謝明君。1998。生羊乳之乳過氧化酵素系統及其延長貯存期之研究。碩士論文,私立東海大學,台中市。
蘇安國、謝瑞春。1999。乳山羊餵飼精粗料完全混合日糧對其泌乳及血液性狀之影響。畜產研究 32:183-191。
Ali, A. K. A., W. A. Mohammad, M. Grossman and R. D. Shanks. 1983. Relationships among lactation and reproduction traits of dairy goats. J. Dairy Sci. 66:1926-1936.
Althaus, R. L., M. P. Molina, M. Rodriguez and N. Fernandez. 2001. Analysis time and lactation stage influence on lactoperoxidase system component in dairy ewe milk. J. Dairy Sci. 84:1829-1835.
Bardsley, W. C. 1985. Steady-state kinetics of lactoperoxidase catalysed reactions. In: The lactoperoxidase system. pp. 55-83. Marcel Dekker. New York.
Barrett, N. E., A. S. Grandison and M. J. Lewis. 1999. Contribution of the lactoperoxidase system to the keeping quality of pasteurized milk. J. Dairy Res. 66: 73-80.
Bibi, W., and M. R. Bachmann. 1997. The rate of thiocyanate in the lactating bovine. Milchwissenschaft 52: 8-10.
Brown, R. W., and M. N. Mickelson. 1979. Lactoperoxidase, thiocyanate and free cystine in bovine mammary secretions in early dry period and at the start of lactation and their effect on Streptococcus agalactiae growth. Am. J. Vet. Res. 40: 250
Browning, R., M. L. Leite-Browning and T. Sahlu. 1995. Factors affecting standardized milk and fat yields in Alpine goats. Small Rumin. Res. 18:173-178.
Carlsson, J., Y. Iwami and T. Yamada. 1983. Hydrogen peroxide excretion by oral Streptococci and effect of lactoperoxidase-thiocyanate- hydrogen peroxide. Infect. Immum. 40: 70-80.
Crepaldi, P., M. Corti and M. Cicogna.1999. Factors affecting milk production and prolificacy of Alpine goats in Lombardy(Italy). Small Rumin. Res. 32:83-88.
Das, M., and M. Singh, 2000. Variation in blood leucocytes, somatic cell count, yield and composition of milk of crossbred goats. Small Rumin. Res. 35:169-174.
De Wit, J. N., and A. C. M. van Hooydonk. 1996. Structure, functions and applications of lactoperoxidase in natural antimicrobial systems. Nether. Milk Dairy J. 50: 227-244.
Devendra, C., and M. Burns, 1983. Goat production in the tropics. pp. 64-73. Commonwealth agricultural bureaux press. London, UK.
Dulin, A. M., M. J. Paape and W. P. Wergin. 1982. Differentiation and enumeration of somatic cells in goat milk. J. Food Prot. 45: 435-439.
Dulin, A. M., M. J. Paape, W. D. Schultze and B. T. Weinland. 1983. Effect of parity, stage of lactation and intramammary infection on concentration of somatic cell and cytoplasmic particles in goat milk. J. Dairy Sci. 66 : 2426-2433.
Egwu, G. O., L. T. Zaria, P. A. Onyeyili, A. G. Ambali, S. S. Adamu and M. Birdling. 1994. Studies on the microbiological flora of caprine mastitis and antibiotic inhibitory concentrations in Nigeria. Small Rumin. Res. 14: 233-239.
Ferrier, L. K., N. F. Olson and T. Richardson. 1970. Analysis of hydrogen peroxide in milk using titanium tetrachloride. J. Dairy Sci. 53: 598-599.
Gipson, T. A., and M. Grossman. 1990. Lactation curves in dairy goats: a review. Small Rumin. Res. 3:383-396.
Goonewardene, L. A., E. Okinea, N. Patrick and H. D. Scheer. 1999. The relationship between multiple births and milk yields in non-suckled intensively managed dairy goats. Small Rumin. Res. 32:181-185.
Haenlein, G. F. W.. 1996. Status and prospects of the dairy goat industry. J. Anim. Sci. 74:1173-1181.
Hanssen, F. S. 1924. The bactericidal property of milk. Br. J. Exp. Pathol. 5: 271-280.
Hogg, D. M., and G. R. Jago. 1970. The antibacterial action of lactoperoxidase; the nature of the bacterial inhibitor. Biochem. J. 117: 779-790.
Jago, G. R., and M. Morrison. 1962. Anti-streptococcal activate of lactoperoxidase. Proc. Soc. Exp. Biol. Med. 111: 585-588.
Juven, B. J., and M. D. Pierson. 1996. Antibacterial effects of hydrogen peroxide and methods for its detection and quantitation. J. Food Prot. 59: 1233~1241.
Klebanoff, S. J., W. H. Clem and R. G. Luebke. 1966. The peroxidase-thiocyanate-hydrogen peroxide antimicrobial system. Biochem. Biophys. Acta 117: 63-72.
Korhonen, H. J., and B. Reiter. 1983. Production of H2O2 by bovine blood and milk polymorphonuclear leucocytes. Acta Microbio. Polo. 32: 53-64.
Kumar, R., and K. L. Bhatia. 1999. Standardization of method for lactoperoxidase assay in milk. Lait 79: 269-274.
Kussendrager, K. D., and A. C. M. van Hooijdonk. 2000. Lactoperoxidase: physico-chemical properties, occurrence, mechanism of action and applications. Brit. J. Nutri. 84: s19-25.
Marshall, V., W. M. Cole and A. J. Bramley. 1986. Influence of the lactoperoxidase system on susceptibility of the udder to Streptococcus uberis infection. J. Dairy Res. 53: 507-514.
Mckellar, R. C., S. Liou and H. W. Modler. 1996. Predictive modeling of lactoperoxidase and glutamyl transpeptidase inactivation in a high-temperature short-time pasteurizer. Int. Dairy J. 6: 295-301.
Mepham, T. B. 1987. Physiology of lactation. Secretory processes. pp. 94-107. Open University press. PA, USA.
Morgante, M., S. Ranucci, M. pauselli, C. Casoli and E. Duranti. 1996. Total and differential cell count in milk of primiparous Comisana ewes without clinical signs of mastitis. Small Rumin. Res. 21 : 265-271.
Nakada, M., S. Dosako, R. Hirano, M. Oooka and I. Nakajima. 1996. Lactoperoxidase suppresses acid production in yoghurt during storage under refrigeration. Int. Dairy J. 6: 33-42.
Nielsen, M. O., and K. Jakobsen. 1993. Changes in mammary glucose and protein uptake in relation to milk synthesis during lactation in high- and low-yielding goats. Comp. Biochem. Physiol. 106: 359-365.
Oram, J. D., and B. Reiter. 1966. The inhibition of Streptococci by lactoperoxidase ,thiocyanate and hydrogen peroxide. The effect of the inhibitory system on susceptible and resistant strains of group N streptococci. Biochem. J. 100: 373-382.
Paape, M. J., B. Poutrel, A. Contreras, J. C. Marco and A. V. Capuco. 2001. Milk somatic cells and lactation in small ruminants. J. Dairy Sci. 84 : E237-244.
Park, Y. W., and R. D. Humphrey. 1986. Bacterial cell counts in goat milk and their correlations with somatic cell counts, percent fat and protein. J. Dairy Sci. 69:32-37.
Paul, K. G., and P. I. Ohlsson. 1985. The chemical structure of lactoperoxidase. In: The lactoperoxidase system. pp. 15-29. Marcel Dekker. New York.
Peris, S., G. Caja and X. Such. 1999. Relationships between udder and milking traits in Murciano-Granadina dairy goats. Small Rumin. Res. 33:171-179.
Peris, S., X. Such and G. Caja. 1996. Milkability of Murciano-Granadina dairy goats. Milk partitioning and flow rate during machine milking according to parity, prolificacy and mode of suckling. J. Dairy Res. 63:1-9.
Poutrel, B., and C. Lerondelle. 1983. Cell content of goat milk: California Mastitis Test, Coulter Counter and Fossomatic for predicting half infection. J. Dairy Sci. 66 : 2575-2579.
Pruitt, K. M., and J. O. Tenovuo. 1985. The lactoperoxidase system. Marcel Dekker, New York. p. 257.
Reiter, B., and R. A. Gibbons. 1964. Some further aspects of the lactoperoxidase-thiocyanate-hydrogen peroxide inhibitory system with special reference to the behaviour of spermatozoa in cervical mucus. Annu. Rep. Natl. Inst. Res. p. 87. Dairying, England.
Reiter, B., V. Marshall, L. Bjorck and C. G. Rosen. 1976. Nonspecific bactericidal activity of the lactoperoxidase-thiocyanate-hydrogen peroxide system on milk against Escherichia coli and some Gram-negative pathogens. Infect. Immun. 13 : 800-813.
Reiter, B., and G. Harnulv. 1984. Lactoperoxidase antibacterial system: natural occurrence, biological functions and practical applications. J. Food Prot. 47 : 724-732.
Reiter, B., and J. P. Perraudin. 1991. Lactoperoxidase: biological functions. In: Peroxidase in chemistry and biology, pp. 143-180. Boca Raton: CRC Press.
Rota, A. M., C. Gonzalo, P. L. Rodriguez, A. I. Rojas, L. Martin and J. J. Tovar. 1993. Somatic cell type in goats milk in relation to total cell count, stage and number of lactation. Small Rumin. Res. 12 : 89-98.
Sanchez, A. J. C., J. Marco and A. Contreras. 1998. Aplicación del recuento de células somáticas para el control de las mamitis capronas. En: “ Mamitis caprinas II ”.Ovis 54: 37-51.
Schoos, S. S., G. Oliver and F. M. Fernandez. 1999. Relationships between lactoperoxidase system components in Creole goat milk. Small Rumin. Res. 32: 69-75.
Stephens, S., R. A. Harkness and S. M. Cockle. 1979. Lactoperoxidase activity in guinea pig milk and saliva: correlation in milk of lactoperoxidase with bactericidal activity against Escherichia coli. Br. J. Pathol. 60: 252-258.
Sung, Y. Y., T. I. Wu and P. H. Wang.1999. Evaluation of milk quality of Alpine, Nubian, Saanen and Toggenburg breeds in Taiwan. Small Rumin. Res. 33: 17-23.
Wilson, D. J., K. N. Stewart and P. M. Sears. 1995. Effects of stage of lactation, production, parity and season on somatic cell counts in infected and uninfected dairy goats. Small Rumin. Res. 16 : 165-169.
Wolfson, L. M., and S. S. Sumner. 1993. Antibacterial activity of the lactoperoxidase system: a review. J. Food Prot. 56:887-892.
Wright, R. C., and J. Tramer. 1958. Factors influencing the activity of cheese starters. The role of milk peroxidase. J. Dairy Res. 25: 104-118.
Ying, C., H. T. Wang and J. T. Hsu. 2002. Relationship of somatic cell count, physical, chemical and enzymatic properties to the bacterial standard plate count in dairy goat milk. Livest. Prod. Sci. 74 : 63-77.
Zapico, P., P. Gaya, M. De Paz, M. Nunez and M. Medina. 1991.Influence of breed, and days of lactation on lactoperoxidase system components in goat milk. J. Dairy Sci. 74: 783-787.
Zeng, S. S., and E. N. Escobar. 1995. Effect of parity and milking method on somatic cell count, standard plate count and composition of goat milk. Small Rumin. Res. 17: 269-274.
Zeng, S. S., and E. N. Escobar. 1996. Effect of breed and milking method on somatic cell count, standard plate count and composition of goat milk. Small Rumin. Res. 19: 169-175.
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