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研究生:林芳村
研究生(外文):Lin , Fang-tsun
論文名稱:銀杏葉萃取物充填聚乳酸-聚甘醇酸共聚合物(PLGA)導管內襯許旺氏細胞於大白鼠坐骨神經再生之肌電圖評估
論文名稱(外文):Assessment of Electromyography on Regeneration of Severed Rat Sciatic Nerve through Schwann cell- seeded PLGA Conduit filled with Ginkgo biloba Extract
指導教授:徐善慧徐善慧引用關係
指導教授(外文):Hsu, Shan-hui
學位類別:碩士
校院名稱:國立中興大學
系所名稱:生命科學院碩士在職專班
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2003
畢業學年度:91
語文別:中文
論文頁數:36
中文關鍵詞:銀杏葉萃取物聚乳酸-聚甘醇酸共聚合物導管許旺氏細胞神經再生肌電圖
外文關鍵詞:Ginkgo biloba ExtractPLGA ConduitSchwann cellNerve RegenerationElectromyography
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依據目前對於神經細胞再生所做的研究,可以得知神經的再生成功與否端賴於Schwann cell能否在神經再生的過程中扮演一個導引的角色。因此在神經導管修補法中,選擇理想的神經導管、結合管壁充填Schwann細胞、加入具有刺激神經再生的物質、配合純熟的外科顯微手術是缺一不可的方法。
本實驗使用20隻體重為250∼300公克健康的雄性SD品系大白鼠。將其右後肢的坐骨神經截斷,形成10mm的缺損後。利用顯微縫合技術,以聚乳酸聚甘醇酸共聚合物導管,充填許旺氏細胞予以修補。所有的大白鼠分成對照組(自體神經組)以及四組實驗組(神經導管組)。在實驗組中,導管分別被灌注0、10、50、100 g/ml不等濃度的銀杏葉萃取液。
所有動物分別在第2、4、6週,進行系列的神經電生理評估--肌電圖檢查。
研究結果發現:代表去神經狀態的動作電位及正相銳波,有逐週下降的趨勢。而代表神經再生的運動單位動作電位,雖然在第2週並未出現,但4週開始出現並逐漸加大振幅。最佳的實驗結果組別濃度為10 g/ml。
本研究結論為銀杏葉萃取液,可以促進周邊神經的再生及功能恢復。而且,使用肌電圖,可以提供神經病變的嚴重程度、發生時間長短、以及其預後生長,是最佳的神經電位生理診斷利器。
In the recent study, Schwann cell guidance channel plays an important role in the nerve regeneration. For this reason, there are some major methods to accomplish a successful nerve conduit study, including choice of ideal conduit, seeding with Schwann cell, skilled microscopic technique, and so on.
In this study, 20 Spraque-Dawley rats weighting 300 ± 50 gm were used. The right sciatic nerves of the rats were transected with gaps of 10mm and bridged with Schwann cell seeded PLGA conduits under microscopic repair. Among the conduits were filled with different concentration experiment groups, of Ginkgo biloba extract (0,10,50,100 mg/ml).
The rats were assigned to our control group (Autologous nerve) and four experiment groups (nerve conduits). All animals were studied with serial needle electromyography (EMG) at the 2nd, 4th and 6th week following nerve section.
In animal sequentially followed, fibrillation potential & positive sharp wave amplitudes varied from week to week. However, the overall trend was amplitude decay. The MUAPs was absent at the 2nd week, but generated at the 4th week and the amplitudes increased gradually. It represents the first sign of regeneration of a previously totally denervated muscle. The best condition used in this experiment was 10 mg/ml of Ginkgo biloba extract.
For the results, the Ginkgo biloba extract could enhance the regeneration and the functional recovery of the peripheral nerve. Besides, needle electromyography could provide the useful information about the severity, duration and prognosis of a nerve lesion. It is the most powerful tool of the electromyography study.
第一章 緒論 1
1-1前言
1-2研究目的
第二章 文獻探討 4
2-1週邊神經的再生 4
一、週邊神經損傷的原因
二、周圍神經損傷的定義及分類
三、神經元傷害後之變化->瓦勒氏退化(Walleriandegeneration)
四、神經的手術修復
2-2 神經導管的究 7
一、許旺細胞與生物材料的複合
二、神經營養因子
三、生物材料
2-3生理評估法 9
一、步徑分析法
二、電氣生理學評估
三、肌電圖Electromyography
第三章 材料與方法(Material and Method) 13
3-1 實驗材料
一、聚乳酸-聚甘醇酸共聚合物(PLGA)神經導管
二、許旺式細胞(Schwann cell)
三、銀杏葉萃取液(EGb761)
3-2 實驗動物模式及計劃 13
一、麻醉方式
二、大白鼠坐骨神經神經導管顯微縫合手術
三、實驗動物大白鼠飼養環境
四、觀察及紀錄實驗六週中變化
五、腓腸肌之針極肌電圖 (EMG)檢查
六、觀察展趾恢復時間及程度
七、觀察神經導管植入後變化及神經再生情形
八、組織切片與染色
九、組織切片的定性分析
十、統計方式
第四章 結果 16
4-1 大白鼠外觀 16
4-2神經導管外觀、腓腸肌重量變化及神經再生的情況 17
一、 神經導管外觀
二、 腓腸肌重量變化
4-3神經再生組織學切片及染色檢查 17
4-4 神經生理評估 18
一、步徑分析試驗(Walking track analysis)
二、電氣生理之肌電圖評估
第五章 討論 25
5-1肌電圖電生理檢查的重要性
5-2統計方式及結果
第六章 結論 32
參考文獻 33
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