本研究中以不同去乙醯度及分子量之幾丁聚醣(chitosan)吸附二種卡馬西藍染料，以減少溶液中卡馬西藍染料之含量。結果顯示幾丁聚醣可以有效吸附溶液中卡馬西藍染料。以卡馬西藍R-250溶液而言，吸附效果最佳之幾丁聚醣為分子量11萬，去乙醯度90%，將0.006g幾丁聚醣置入10ml溶液中，在30分鐘內約可吸附83%之色度。以卡馬西藍G-250溶液而言，吸附效果最佳之幾丁聚醣為分子量55萬，去乙醯度90%，將0.006g幾丁聚醣置入10ml溶液中，在15分鐘內約可吸附80%之色度。本研究之結論顯示幾丁聚醣可以有效吸附溶液中的卡馬西藍染料。

　　The purpose of this study is to use chitosan to adsorb coomassie blue aqueous solutions. Our data showed that chitosan adsorb coomassie blue in a dose response manner. When chitosan with degree of deacteylation (DD) value of 90% and molecular weight of 110,000 at concentration of 6 mg/10ml can adsorb 83% of coomassie blue R-250 from the solution in 30 min. When chitosan with DD value of 90% and Mw of 550,000 at concentration of 6 mg/10ml can adsorb 80% of coomassie blue G-250 from the solution in 15min. This report demonstrates that chitosan can be used to effectively adsorb coomassie blue from its aqueous solutions.

中文摘要 Ⅰ
英文摘要 Ⅱ
目錄 Ⅲ
圖目錄 Ⅵ
表目錄 Ⅶ

壹、緒論
　　1-1前言 1
　　1-2研究動機 1

貳、文獻探討
　　2-1生體高分子(Biopolymer)的發展 3
　　2-2甲殼廢棄物之處理 3
　　2-3幾丁質與幾丁聚醣的特性與製備 4
　　　2-3-1幾丁質與幾丁聚醣的特性 4
　　　2-3-2幾丁質與幾丁聚醣的製備 6
　　　　2-3-2.1幾丁質的製備 6
　　　　2-3-2.2幾丁聚醣的製備 7
　　2-4幾丁質與幾丁聚醣的應用 9
　　　2-4-1生醫材料之應用 10
　　　2-4-2藥物傳輸系統之應用 12
　　　2-4-3機能性食品之應用 13
　　　2-4-4農業上的利用 17
　　　2-4-5食品加工及紡織上之應用 17
　　　2-4-6環境保護上的利用 18
　　2-5幾丁質與幾丁聚醣產品國內外市場分析 19
　　　2-5-1幾丁質與幾丁聚醣產品行銷通路 19
　　　2-5-2幾丁質與幾丁聚醣產品國外市場與未來動向 21
　　　2-5-3幾丁質與幾丁聚醣產品國內市場概況 22
　　2-6吸附理論 22
　　2-7卡馬西藍染料 23

參、實驗材料與方法
　　3-1藥品與設備 26
　　　3-1-1藥品 26
　　　3-1-2設備 26
　　3-2實驗方法 27
　　　3-2-1不同去乙醯度幾丁聚醣之製備 27
　　　3-2-2不同分子量幾丁聚醣之製備 27
　　　3-2-3幾丁聚醣去乙醯度之測定 27
　　　　3-2-3-1 Ultraviolet Spectrophotometer法(UV法) 28
　　　　3-2-3-2 Infrared Spectroscopy法(IR法) 29
　　　3-2-4幾丁聚醣分子量的測定 30
　　　3-2-5二種卡馬西藍染料檢量線之製作 31
　　　3-2-6不同反應時間之吸附實驗 31
　　　3-2-7不同粒徑之幾丁聚醣濃度對吸附影響之測定 32
　　　3-2-8不同幾丁聚醣濃度對吸附影響之測定 32
　　　3-2-9卡馬西藍染料之脫附實驗 33

肆、結果與討論
　　4-1幾丁聚醣去乙醯度之測定 36
　　　4-1-1 Ultraviolet Spectrophotometer法(UV法) 36
　　　4-1-2 Infrared Spectroscopy法(IR法) 36
　　4-2幾丁聚醣分子量的測定 36
　　4-3二種卡馬西藍染料之檢量線 37
　　4-4不同反應時間之吸附實驗測定 37
　　4-5不同去乙醯度、不同分子量之幾丁聚醣在不同反應時間之吸附實驗測定 38
　　4-6不同粒徑之幾丁聚醣對吸附影響的測定 39
　　4-7不同濃度之幾丁聚醣吸附能力的測定 39
　　4-8卡馬西藍染料之脫附實驗 40

伍、結論 41

陸、參考文獻 42

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