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研究生:蔡銘哲
研究生(外文):Ming-Che Tsai
論文名稱:苯胺系列化合物對假單胞菌的抑制行為
論文名稱(外文):The Inhibition Behavior of Aniline Derivatives Compounds to Pseudomonas putida
指導教授:黃守仁黃守仁引用關係黃得時黃得時引用關係
指導教授(外文):Shou-Ren HuangDe-Shi Huang
學位類別:碩士
校院名稱:國立成功大學
系所名稱:化學系碩博士班
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2003
畢業學年度:91
語文別:中文
論文頁數:57
中文關鍵詞:結構活性定量關係瞬時耗氧速率假單胞菌苯胺衍生物
外文關鍵詞:Initial Oxygen UptakePseudomonas putidaAniline DerivativesQSAR
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芳香族化合物常不經意的被排放至水域環境當中,造成生態環境的
毒害,為了進一步的防範與處理,勢必要先了解化合物的毒性強弱。
化合物的抑制機制有三種類型,而苯胺系列化合物對假單胞菌的抑
制皆屬於非競爭型抑制劑,所以當結合物理參數與實驗所得的抑制常數值則可建造出一套系統-結構活性定量關係,對於日後則可以迅速的被用來去評估化合物相對毒性強弱。
利用氧電極偵測假單胞菌會在不同濃度的受質與抑制劑下,氧消耗
的初始速率,藉由動力學模式求得化合物對假單胞菌的抑制常數值,此值表示當假單胞菌受抑制劑抑制達50%時濃度時的速率常數值。
藉由此方法,將實驗所得的抑制常數值與Tetrahymena pyriformis做
比對發現其有高度的相關性(R2=0.90)。此方法具有迅速、花費少、精準性與再現性好等優點。
Abstract
The toxicity of aniline derivatives was assessed by employing the
Pseudomonas initial oxygen uptake (PIOU) method. Based on enzymatic kinetics, there are three types of mechanism for the inhibition of chemicals to the oxygen-dependent Pseudomonas. The inhibition constant, Ki, of aniline derivatives were measured and found that most aniline derivatives are belonging to the non-competitive inhibitors to Pseudomonas putida. These Ki constant represent the concentration of the aniline derivatives, under which 50% of the oxygen uptake activity of Pseudomonas putida has been inhibited.

The inhibition constant Ki obtained in this study was correlated with both
the n-octanol/water partition coefficient (log p) and the energy of lowest unoccupied molecular orbital (LUMO) to establish a quantitative structure-activity relationship (QSAR) model and found that this established equation allow one to predict the inhibition mechanism toward P. putida.. Comparing the Ki data in this studies with the EC50 data from Tetrahymena pyriformi database, it was found that a highly correlation (R2=0.90) was obtained. This study shows that PIOU method was proven to be a rapid, economic, precision, and reliable method.
中文摘要……………………………………………………………….……..i
英文摘要……………………………………………………………….…….ii
誌謝………………………………………………………………………….iii
目錄………………………………………………………………………….iv
表目錄……………………………………………………………………….vi
圖目錄………………………………………………………………………vii
附錄………………………………………………………………………...viii
第一章緒論…………………………………………………………………1
一、生物分解應用於毒性評估……………………………………….…….1
二、利用生物測試化合物毒性的方法…………………………….……….3
三、假單胞菌的簡介……………………………………………………….11
四、結構-活性定量關係(QSAR)的介紹…………………………….…….12
五、苯胺系列化合物………………………………………………………18
六、化合物作用的毒性機制………………………………………………19
七、實驗目的………………………………………………………………20
第二章實驗………………………………………………………………..21
一、實驗藥品………………………………………………………….…...21
二、實驗儀器………………………………………………………….…...23
三、實驗內容………………………………………………………….…...25
(一)細菌的培養及處理………………………………………………..25
(二)實驗流程…………………………………………………………..26
(三)氧電極測試………………………………………………………..28
(四)數據處理…………………………………………………………..31
第三章結果與討論……………………………………………………….37
一、化合物的Ki值………………………………………………………..37
二、化合物毒性與結構分析……………………………………………...39
三、物理參數對毒物的關係……………………………………………...39
四、討論…………………………………………………………………...42
(1) 氧電極測試法……………………………………………………..42
(2) Comparative QSAR………………………………………………..43
(3) Compare with Pimephales promelas………………………………45
(4) 偏離點的分析……………………………………………………..46
五、結論…………………………………………………………………...47
參考文獻…………………………………………………………………...48
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