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研究生:楊媛婷
研究生(外文):Yuan-Ting Yang
論文名稱:利用單株抗體研究人類血紅素結合蛋白(Haptoglobin)的免疫化學結構及其在臨床上的應用
論文名稱(外文):Immunochemical structure of human haptoglobin as probed by monoclonal antibodies and its clinical implications
指導教授:毛仁淡
指導教授(外文):SJT Mao
學位類別:碩士
校院名稱:國立交通大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2003
畢業學年度:91
語文別:中文
論文頁數:60
中文關鍵詞:單株抗體
外文關鍵詞:Haptoglobinacute phase proteinmonoclonal antibody
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人類的血紅素結合蛋白 (haptoglobin) 分為三種亞型: Hp 1-1,Hp2-2,以及Hp 2-1。Hp 1-1為單一分子構造,由兩個α1 subunit及兩個β subunit 經雙硫鍵鍵結所構成,Hp2-1及Hp 2-2則是以上述鍵結方式分別聚合α1,α2及β等subunits以多聚體的構造出現於人體中。血紅素結合蛋白和人類的許多具發炎反應之疾病有關,因此在臨床檢測是一有效的指標蛋白質。
本研究中成功地研製出六株haptoglobin的單株抗體,分別命名為8B1、2H3、W1、4A2、G2D及12B1。六株單株抗體中,對於haptoglobin結構中的β subunit具有極高特異性 (specificity) 者有五株,僅W1對α subunit具有特異性。Subtyping測試結果得知這六株抗體均歸屬於IgG1。探討haptoglobin的免疫活性過程中,我們利用單株抗體進行competitive ELISA,結果顯示Hp1-1的免疫活性大於其他haptoglobin亞型;另外,將haptoglobin甲基化改變其雙硫鍵結構做進一步探討,發現G2D單株抗體之免疫活性不受甲基化影響,且可以辨識經過trypsin切過的片段;藉此證據進而推測G2D無法辨識haptoglobin雙硫鍵鍵結之蛋白質相關結構。同時,利用單株抗體進行sandwich ELISA所得之標準定量曲線可以適當地應用於臨床haptoglobin的定量標準。
There are 3 major phenotypes of human haptoglobin (Hp), designated as 1-1, 2-2, and 2-1, respectively. The simplest form Hp 1-1 is a tetrachain structure composed of 2 α1 and 2 β chains linked by disulfide linkages. Whereas, Hp 2-2 and 2-1 are polymerized forms of higher molecular mass. Genetically, there is still no generally accepted or satisfactory mechanism explaining the functional maintenance of genetic polymorphism in human populations.
Six monoclonal antibodies (mAb): 8B1, 2H3, W1, 4A2, G2D, and 12B1, prepared against human Hp 2-1 were produced, purified, and characterized. The specificity of each mAb evaluated by Western blot shows that five of the mAb were specific to the β-chain of Hp. Whereas, only mAb W1 recognized α 1 and α 2 subunits of Hp. Each mAb was then subject for purification on a DEAE-column. Notably, the purity of each mAb was greater than 90%. Subtyping indicated that they were all IgG1 subclass.
To determine the role of cysteine in maintaining the immunochemical structure of Hp, Hp 1-1, 2-1, and 2-2 phenotypes were irreversibly modified by carboxymethylation to block all the disulfide linkages between the α- and β-chains as well as the inter-molecular linkages between each monomer of Hp 2-1 or 2-2. It was of significant interest that one of the β-chain monoclonal antibodies (G2D) possessed the immunoreactivity that was not affected by the blockage of disulfide linkages and trypsin fragmentation of Hp. Since Hp 1-1 represents a monomeric unit of Hp (α1β)2, we speculated that the antigenic epitopes might be more accessible for the antibody binding than that of polymeric Hp 2-1 or 2-2. To test this hypothesis, we determined the immunoreactivity of each phenotype of Hp in a competitive ELISA. We demonstrated that the immunoreactivity of Hp 1-1 was significantly greater (5x) than that of Hp 2-1 and 2-2 using mAb 2H3.
We conclude that: (1) intra-molecular disulfide linkages play an essential role in maintaining the antigenic structure of Hp; (2) Hp 1-1 appears to possess higher immunoreactivity then that of polymeric form Hp 2-1 and Hp 2-2. It further suggests the notion that Hp 1-1 provides more surface area and hence an increase in biological activity.
Contents------------------------ i
Abbreviation---------------------- ii
Abstract------------------------ iii
Introduction---------------------- 1
Materials and Methods------------------ 5
Results------------------------ 26
Discussion----------------------- 31
References----------------------- 35
Table------------------------- 39
Figure------------------------- 45
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