(3.235.41.241) 您好!臺灣時間:2021/04/15 03:54
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:左榮昇
研究生(外文):Jung-Chen Cho
論文名稱:四種蟳大眼幼體在外部型態及族群動態上之差異
論文名稱(外文):Differences in External Morphology and Population Dynamics among theMegalopae of Four Scylla species
指導教授:鄭金華
指導教授(外文):Jin-Hua Cheng
學位類別:碩士
校院名稱:國立中山大學
系所名稱:海洋生物研究所
學門:自然科學學門
學類:海洋科學學類
論文種類:學術論文
論文出版年:2002
畢業學年度:91
語文別:中文
論文頁數:76
中文關鍵詞:型態脫殼大眼幼體
外文關鍵詞:megalopamorphologyScyllamolt
相關次數:
  • 被引用被引用:3
  • 點閱點閱:260
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:39
  • 收藏至我的研究室書目清單書目收藏:0
本研究針對東港附近海域捕獲四種蟳之大眼幼體及第一期稚蟹之外部型態上詳加描述,並比較其差異,以作為鑑種的依據。花腳蟳大眼幼體在第五腹節具有指狀下緣,與其他三種明顯不同。紅腳蟳大眼幼體在左右第一觸角基節的剛毛數均為4, 4的組合,與其他三種的3, 3,3, 4或4, 3的組合明顯不同。貴蟳大眼幼體在第二觸角最後兩節之剛毛數為4, 5的組合,與其他三種均為3, 3的組合明顯不同。其次,四種蟳大眼幼體在第五泳足、第三顎足及第五步足都有較明顯的差異。另外,四種蟳大眼幼體在體型大小上亦有顯著差異,以額寬與頭胸甲寬為例紅腳蟳最大、花腳蟳次之、白腳蟳第三、貴蟳最小。在第一期稚蟹的形態描述亦發現:中央額齒及螯腳就如其成蟹在種間有明顯的差異。
在大眼幼體族群動態方面,本研究於1999年9月至2000年9月,自林邊溪口外海、林邊溪口海岸、番子崙外海及番子崙海岸等地採集大眼幼體。利用multiplex PCR技術大量鑑定大眼幼體的種別。在上述調查期間發現:白腳蟳最多,占67.7±21.5%;花腳蟳次之,占19.3±15.5%;紅腳蟳第三,占12.3±11.9%;而貴蟳最少,占0.7±1.7%。蟳大眼幼體之脫殼大部分發生在夜間,占77.1%。大眼幼體到達稚蟹所需要的天數自沿岸捕獲者比自外海捕獲者少一天(p<0.001),白腳蟳較紅腳蟳及花腳蟳多一天(p<0.001)。鹽度 (15-30 ppt) 對四種蟳大眼幼體的脫殼時程沒有影響(p>0.29)。
The purpose of this research is to describe and to compare the morphology of megalopa and the first crab of four Scylla species which lives along the coast of Tungkang. The megalopa of S. serrata has dactyl lower margin on the fifth abdominal segment which is different from three other Scylla species. S. olivacea has setae number of 4,4 combination on both sides of the basal segment of antenna. It is different from three other species which are either “ 3,3” or “ 3,4” or “ 4,3” combination. S. tranquebarica has setae number of 4,5 combination on the last two segments of antennule. It is different from three other species, which have 3,3 combination. In addition, these four kinds of megalopa are different on the fifth pleopod, third maxilliped and fifth pereiopod. Furthermore, megalopa of four Scylla species have significant differences in their shape and size. For example, S. olivacea is the largest one in both rostral width and carapace length, followed by S. serrata, S. paramamosain, and S. tranquebarica is the smallest one. In the first crab, as adult,
significant differences are found on the frontal median spines and chelipeds.
From September 1999 to September 2000, megalopae of four Scylla species were collected from the coast and offshore of Linpien and Fanzilum. Large amount of megalopa can be identified in to species using of one-step multiplex PCR. Results showed that S. paramamosain is the most abundant (67.7±21.5%) , followed by S. serrata (19.3±15.5%), S. olivacea (12.3±11.9%), and S. tranquebarica (0.7±1.7%) is the least one. Most of the megalopa (77.1%) molted during the night. The megalopa caught from coast took one day (p<0.001) less to become the first crab than those caught from offshore. S. paramamosain took one day more than S. serrata and S.
olivacea.Salinity doesn’t affect the time of molt.
目錄
章次 頁數
中文摘要 ……………………………………………………. III
英文摘要 ……………………………………………………. IV
謝辭 …………………………………………………………. V
目錄 …………………………………………………………. VI
表目錄 ………………………………………………………. VIII
圖目錄 ………………………………………………………. IX

一、前言 ………………………………………………. 1
二、材料與方法 ………………………………………. 4
I. 材料收集及處理 ………………………………. 4
II. 大眼幼體及第一期稚蟹外部形態之描述及比較……………………………………………... 4
III. 分生鑑種 …………………………………….. 6
a. chelex-100 resin 萃取法 …………………... 6
b. Multiplex PCR ……………………………… 7
c. 洋菜膠電泳分析 …………………………... 7
IV. 大眼幼體外部形態形質分析…………………. 8
V. 四種蟳大眼幼體之分布頻度與脫殼試驗 ..…………………………………………... 8
a.四種蟳大眼幼體的分布頻度 ………….…… 8
b.鹽度對大眼幼體脫殼之影響 ..…………….. 8
三、結果 ………………………………………………. 10
I. 四種青蟹大眼幼體外部形態及形質 ………… 10
章次 頁數
a. 貴蟳 ………………………………………... 10
b. 白腳蟳 …………………………………….. 11
c. 紅腳蟳 …………………………………….. 12
d. 花腳蟳 …………………………………….. 14
e. 四種青蟹大眼幼體之比較 ……………….. 15
f. 四種青蟹大眼幼體外部形態形質分析 …... 17
II. 四種青蟹第一期稚蟹外部形態 ……………… 17
a. 貴蟳 ………………………………………... 17
b. 白腳蟳 …………………………………….. 18
c. 紅腳蟳 …………………………………….. 20
d. 花腳蟳 …………………………………….. 21
e. 四種青蟹第一期稚蟹之比較 …………….. 22
III. 脫殼實驗 …………………………………….. 23
a. 海岸及外海脫殼實驗 ……………………... 24
b. 不同鹽度脫殼實驗 ……………………….. 24
c. 光照脫殼實驗 ……………………………... 24
IV. 蟳之生態分布 ……………………………….. 24
四、討論 ……………………………………………….. 26
五、參考文獻 ………………………………………….. 29
參考文獻
林仲惠,1993。以形態生化及行為特徵探討鋸緣青蟹之分類地位及族群關係之研究。國立海洋大學漁業研究所碩士學位論文。
黃丁郎、洪金抱,1971。蟳養殖。Aquiculture.1, 31-36。
黃榮富,1993。台灣產梭子蟹類之分類及分布研究。國立台灣大學漁業科學研究所博士學位論文。
施心展,1999。四種青蟹(Scylla spp.)在外部型態上及核糖體DNA上之差異。國立中山大學海洋生物研究所碩士學位論文。
陳溫柔,1995。東港沿岸蟹類大眼幼體之分類研究。國立中山大學海洋生物研究所碩士學位論文。
鄭金華、陳紫女英、吳慧娜、蘇茂森,1993。三種蟳苗之季節性頻度及其外部型態之分析。台灣省水產學會論文發表會摘要。58。
Antonio Rodriguez and Joel W. Martin. 1997. Larval development of the crab Xantho poressa (Decapoda: Xanthidae) reared in the laboratory. Journal of Crustacean Biology, 17(1): 98-110.
Chang, H. K. and S. G. Hwang. 1995. The complete larval development of the mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Decapoda, Brachyura, Grapsidae) reared in the laboratory and a key to the known zoeae of the Varunidae. Crustaceana, 68: 793-812.
Chen, W. J., J. H. Cheng and K. Y. Soong. 2000. On the Megalopae of three species of crab (Crustacea: Decapoda: Brachyura: Calappidae, Matutidae) from Tungkang, Southwestern Taiwan. Journal of the National Taiwan Museum, 53: 23-35.
Clark, P. F., D. K. Calazans and G. W. Pohle. 1998. Accuracy and standardization of brachyuran larval descriptions. Invertebrate Reproduction and Development, 33: 127-144.
Estampador, E. P. 1949. Scylla (Crustcea: Portunidae) II. Comparative studies on spermatogenesis and oogenesis. Philippine Journal of Science, 78: 301-353.
Felder, D, L., J. W. Martin and J. W. Goy. 1985. Patterns in early postlarval development of decapods. Pages 163-225 in A. M. Wenner, editor. Larval Groeth. Boston. Pp. 1-236.
Francesca Gherardi and Patsy A. McLaughlin. 1995. Larval and early juvenile development of the tube-dwelling hermit crab Discorsopa- gurusschmitti (Stevens) (Decapoda: Anomura: Paguridae) reared in the laboratory. Journal of Crustacean Biology, 15: 258-279.
Fuseya, R. and S. Watanabe. 1996. Genetic variability in the mud crab genus Scylla (Brachyura: Portunidae). Fisheries Science, 62:705 -709.
Guo, J. Y., N. K. Ng, A. Dai and K. L. Peter. 1997. The taxonomy of three commercially important species of mitten crabs of the genus Eriocheir De Hann, 1835 (Crustacea: Decapoda: Brachyura: Grapsidae). The Raffles Bulletin of Zoology, 45: 445-476.
Guo, J. Y., N. K. Ng, A. Dai and K. L. Peter. 1998. The complete larval development of the southern Chinese mitten crab, Eriocheir hepuensis Dai, 1991 (Decapoda, Brachyura, Grapsidae) reared under laboratory conditions. Crustaceana, 71: 493-517.
Hyun S. K. 1995. Larval development of Benthopanope indica (de Man, 1887) (Decapoda: Brachyura: Pilumnidae) in the laboratory. Journal of Crustacean Biology, 15: 280-290.
Hill, B. J. 1994. Offshore spawning by the portunid crab Scylla serrata (Crustacea: Decapoda). Marine Biology, 120:379-384.
Keenan, C. P., P. J. F. Davie and D. L. Mann. 1995. Genetic and morphological relationship of mud crabs, genus Scylla, from throughout the Indo-Pacific. ACIAR Project Report. QDPI. Brisbane.
Keenan, C. P., P. J. F. Davie and D. L. Mann. 1998. A revision of the genus Scylla de-Haan, 1833 (Crustacea, Decapoda, Brachyura, Portunidae). Raffles Bulletin of Zoology, 46: 217-245.
Keenan, C. P., J. M. Hughes and D. Gopurenko. 1999. Mitochondrial DNA evidence for rapid colonisation of the Indo-West Pacific by the Mudcrab Scylla serrata. Marine Biology, 134: 227-233.
Kim, C. H. and S. G. Hwang. 1990. The complete larval development of Eriocheir japonicus De Haan (Crustacea, Brachyura, Grapsidae) reared in the laboratory. Korean Journal of Zoology, 33: 411-427.
Klinbunga, S., A. Boonyapakdee and B.Pratoomchat. 2000. Genetic diversity and species-diagnostic markers of mud crabs (genus Scylla) in eastern Thailand determined by RAPD analysis. Marine Biotechology, 2: 180-187.
Montu, M., K. Anger and C. de Bakker. 1996. Larval development of the Chinese mitten crab Eriocheir sinensis H. Milne-Edwards (Decapoda Grasidae) reared in the laboratory. Hengolander Meeresuntersuchungen, 50: 223-252.
Ong, K. S. 1964. The early developmental stages of Scylla serrata Forskal (Crustacea Portunidae), reared in the laboratory. Proceeding Indo-Pacific Fish Congress, 11: 135-146.
Overton, J. L., D. J. Macintosh and R. S. Thorpe. 1997. Multivariate analysis of the mud crab Scylla serrata (Brachyura: Portunidae) from four locations in Southeast Asia. Marine Biology, 128: 55-62.
Shy, J. Y. and H. P. Yu. 1992. Complete larval development of the mitten crab Eriocheir rectus Stimpson, 1858 (Decapoda, Brachyra, Grapsidae) reared in the laboratory. Crustaceana, 63: 277-290.
Walsh, P. S., D. A. Metzger and R. Higuchi. 1991. Chelex 100 as a medium for sample extraction of PCR-base typing from forensic material. Biotechniques, 10: 506-513.
Yu, H. P. 1979. The swimming crabs (Crustacea, Decapoda, Portunidae) of Taiwan. Aquaculture, 2: 41-74.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關期刊
 
系統版面圖檔 系統版面圖檔