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研究生:江清瑩
研究生(外文):Ching-Ying Chiang
論文名稱:GN51傘軟珊瑚化學成分及癌細胞毒殺活性之研究
論文名稱(外文):Chemical Constituents and Cytotoxicity of Formosan Soft Coral Xenia sp. (GN51)
指導教授:杜昌益杜昌益引用關係
指導教授(外文):Chang-Yih Duh
學位類別:碩士
校院名稱:國立中山大學
系所名稱:海洋資源學系研究所
學門:自然科學學門
學類:海洋科學學類
論文種類:學術論文
論文出版年:2003
畢業學年度:91
語文別:中文
論文頁數:127
中文關鍵詞:傘軟珊瑚
外文關鍵詞:Xenia
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綠島產 (Xenia sp.) (GN51)傘軟珊瑚之有機層萃取物經層析分離後得到12個化合物,分別為isoxeniolide-A (1), xeniolide-B (2), 4b -hydroxgermacra-1(10),5-diene (3), parahigginone (4), GN51-118 (5), GN51-119 (6), floride A (7), xenione (8), 13-epi-9-desacetyl-xenicin (9), xenitorin C (10), xenitorin A (11), xenitorin B (12)。其中GN51-118 (5)為新骨架的化合物, GN51-119 (6)為新化合物。

上述化合物經癌細胞毒殺活性測試後,xeniolide-B (2), xenione (8), 13-epi-9-desacetyl-xenicin (9)對P-388、HT-29細胞株均有顯著的抑制作用,化合物5對P-388細胞株有抑制作用。
Organisms of Formosan soft coral Xenia sp. (GN51) (collected at green island off Taiwan) were freeze-dried to give 600 g of a solid, which was extracted with CH2Cl2 and CH2Cl2/MeOH (1:1). After removal of solvent in vacuo, the residue was chromatographed over Sical gel 60 separation resulated in the isolation of two new diterpenes, GN51-118 (5) (new carbon skeleton) and GN51-119 (6), five known diterpenes isoxeniolide-A (1), xeniolide-B (2), floride A (7), xenione (8), 13-epi-9-desacetyl-xenicin (9) as well as five sesquiterpenes 4b- hydroxgermacra-1(10),5-diene (3), parahigginone (4), xenitorin C (10), xenitorin A (11)and xenitorin B (12)。
目錄
頁次
壹、 緒論 1
一、前言 1
二、Xenia屬軟珊瑚之天然物化學研究回顧 2~23
三、研究動機及目的 24
貳、 GN51傘軟珊瑚化學成分之研究
一、材料採集與鑑定 25
二、GN51傘軟珊瑚之分類地位 25
三、GN51傘軟珊瑚之萃取與分離 26~27
四、GN51傘軟珊瑚化學成分之分離純化 28
(1) GN51-181之分離純化 28~30
(2) GN51-183之分離純化 31~33
(3) GN51-47之分離純化 34~36
(4) GN51-49之分離純化 37~39
(5) GN51-118之分離純化 40~42
(6) GN51-119之分離純化 43~45
(7) GN51-120之分離純化 46~48
(8) GN51-138之分離純化 49~51
(9) GN51-139之分離純化 52~54
(10) GN51-121之分離純化 55~57
(11) GN51-122之分離純化 58~60
(12) GN51-123之分離純化 61~63
五、GN51傘軟珊瑚化學成分之結構解析
(1) GN51-181之結構解析 64~68
(2) GN51-183之結構解析 75~78
(3) GN51-47之結構解析 85~88
(4) GN51-49之結構解析 95~99
(5) GN51-118之結構解析 108~113
(6) GN51-119之結構解析 123~128
(7) GN51-120之結構解析 135~136
(8) GN51-138之結構解析 139~142
(9) GN51-139之結構解析 149~153
(10) GN51-121之結構解析 160
(11) GN51-122之結構解析 163
(12) GN51-123之結構解析 166

参、 GN51傘軟珊瑚所分出化合物之細胞毒殺活性 169
肆、 結論 170
伍、 參考文獻 171~176
陸、 附錄
一、 儀器及材料 177~178
二、 癌細胞毒殺活性測試之分析方法 179
三、 MTT 分析原理 180
伍、參考文獻
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