(3.237.20.246) 您好!臺灣時間:2021/04/15 12:01
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:張衿菱
研究生(外文):Chang Chin Ling
論文名稱:探討CDK抑制劑抑制LPS誘導microglialcell發炎反應及NO誘導細胞凋亡之作用機制
論文名稱(外文):Cdk inhibitors, olomoucine and roscovitine,reduce pro-inflammatory responses and NO-induced apoptosis in BV-2 microglial cell
指導教授:唐世杰
指導教授(外文):Tang Shye-Jye
學位類別:碩士
校院名稱:國立海洋大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2003
畢業學年度:91
語文別:中文
論文頁數:81
中文關鍵詞:細胞生長抑制劑一氧化氮細胞凋亡酯質多醣類微膠細胞
外文關鍵詞:CDKINOapoptosisLPSBV-2
相關次數:
  • 被引用被引用:1
  • 點閱點閱:383
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:38
  • 收藏至我的研究室書目清單書目收藏:0
人類神經退化疾病Alzheimer’s、prion disease、Parkinson’s、Huntington’s diseases的分子機制並不完全了解。但2個主要神經疾病病理上的改變為神經細胞死亡及出現活化的microglial cells,microglial為常駐在中樞神經系統中的macrophage,microglial cells被活化會釋放許多cytokines:TNF-α、IL-1β、也產生發炎相關的酵素:i NOS。及reactive oxygen species (ROS)。microglial cells適當的活化有益於保護神經系統,而異常活化會造成神經系統損壞。因此本論文選用BV-2當作學習的模式,使用LPS誘使BV-2細胞發炎,及SNAP促使BV-2細胞凋亡,發現LPS誘導NO產生會被JNK、P38、PI3K的抑制劑給抑制,表示JNK、P38、PI3K途徑參與LPS誘導NO產生,先處理CDK抑制劑可藉著抑制JNK的活化而達到抑制LPS誘導之i NOS m RNA、i NOS蛋白質、NO的產生;CDK抑制劑也會藉著抑制TNF-α降解以抗NO所誘導之細胞凋亡.
The molecular mechanisms leading to human neurodegenerative diseases such as prion diseases, Alzheimer’s, Parkinson’s, and Huntington’s diseases are not well understood. Two of the major neuropathological changes that characterize these diseases are neuronal cell apoptosis and the presence of activated microglial cells. Microglia are resident macrophages with wide distribution in nervous tissues. The activation of microglial cells in response to pathological stimuli is associated with the production of various cytokines, such as TNF-α and IL-1β, and the induction of inflammation-related enzymes such as inducible nitric oxide synthase (iNOS). Proper activation of microglial cells may be beneficial for the neuroprotective processes, but an abnormal activation of microglia, may become dangerous to nervous tissues. We studied in LPS-stimulated BV-2 cells as a model of microglia activation and SNAP-stimulated BV-2 cells as a model of microglia apoptosis. The LPS-induced production of NO was inhibited by the selective p38 MAPK inhibitor SB203580、JNK inhibitor SP600125、PI3K inhibitor LY294002. These results indicated that the p38 MAPK、JNK、PI3K signaling pathway was involved in the LPS-induced NO production. CDKI suppressed the phosphorylation of JNK and resulted in a reduced in LPS-induced nitrite, iNOS mRNA and protein levels. CDKI suppressed the TNF-α degradation and resulted in a reduced in the NO-induced apoptosis.
目錄……………………………………………………………………….1
謝誌……………………………………………………………………….4
中文摘要………………………………………………………….....…5
英文摘要…………………………………………………………......6
縮寫表…………………………………………………………………...7
壹、序論…………………………………………………………..…..8
1.microglial cells在細胞生理的功能…………………………...8
2.microglia活化與Alzheimer’s diseases的係………………….8
3.LPS產生的發炎反應之訊號傳遞…………………………………….8
4.LPS活化microglia細胞…………………………………….………….9
5.NO與細胞凋亡…………………………………………………..10
6.細胞週期的調控…………………………………………………..…10
7.細胞週期抑制劑…………………………………………….…….…11
8.制劑細胞週期與細胞的生理反應…………………………….….….12
9.實驗目的………………………………………………………..…….12
貳、實驗材料..……………………………………………….…….13
1.細胞株(Cell line)………………………………….……..13
2.酵素(Enzyme)………………………………………………….13
3.PCR primer…………………………………..………………..……13
4.Kit………………………………………………………….………….14
5.Marker………………………………………………………………..14
6.抗體(antibody)………………………………………………..…..14
7.細胞培養(Cell culture)………………………………………...…15
8.chemical…………………………………………………………..…..15
叁、方法………………….……………………………………...16
1.(MTS)細胞數測定………………………………………………..16
2.RNA純化………………………………………………………….…16
3.反轉錄反應(Reverse Transcription, RT)………………….17
4.聚合酵素連鎖反應…………………………………………….17
5.細胞破碎……………………………….…………………19
6.SDS-PAGE…………………….………………………….…..…..19
7.西方點墨法(Western blotting)………………………..……..21
8.細胞分化測定…………………………………………………….…….22
肆、結果…………………………………………………………...23
伍、討論….………………………………………………...32
陸、參考文獻………………….……………..….37
柒、附 圖…………………………………………………..…...48
Abraham RT, Acquarone M, Andersen A, Asensi A, Belle R, Berger F, Bergounioux C, Brunn G, Buquet-Fagot C and Fagot D(1995)Cellular effects of olomoucine, an inhibitor of cyclin-dependent kinases. Biol Cell. 83:105-120.
Barger SW, Horster D, Furukawa K, Goodman Y, Krieglstein J and Mattson MP (1995) Tumor necrosis factors alpha and beta protect neurons against amyloid beta-peptide toxicity: evidence for involvement of a kappa B-binding factor and attenuation of peroxide and Ca2+ accumulation. Proc Natl Acad. Sci.U.S.A. 92:9328—9332
Binetruy B, Smeal T and Karin M (1991) Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain Nature 351:122—127
Bitting L, Naidu A, Cordell B and Murphy GM Jr (1996) Beta-amyloid peptide secretion by a microglial cell line is induced by beta-amyloid- (25-35) and lipopolysaccharide. J. Biol. Chem.271:16084—16089
Blasi E, Barluzzi R, Bocchini V, Mazzolla R and Bistoni F (1990) Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus. J. Neuroimmunol. 27:229-237.
Boulet I, Ralph S, Stanley E, Lock P, Dunn AR, Green SP and Phillips WA(1992.)Lipopolysaccharide- and interferon—gamma—induced expression of hck and lyn tyrosine kinases in murine bone marrow -derived macrophages. Oncogene 7:703-710.
Busse R and Mulsch A (1990) Calcium-dependent nitric oxide synthesis in endothelial cytosol is mediated by calmodulin. FEBS Lett. 265:133—136.
Carter AB, Monick MM and Hunninghake GW (1999) Both Erk and p38 kinases are necessary for cytokine gene transcription. Am. J. Respir .Cell Mol. Biol. 20:751—758
Chan FK, Zhang J, Cheng L, Shapiro DN and Winoto A.(1995)Identification of human/mouse p19, a novel CDK4/CDK6 inhibitor with homology to p16ink4. Mol. Cell. Biol. 15:2682—2688.
Charalambidis ND, Foukas LC and Marmaras VJ(1991)Bacterial lipopolysaccharide stimulates protein tyrosine phosphorylation in macrophages. Proc. Natl. Acad. Sci. USA 88:4148-4152.
Chen J, Saha P, Kornbluth S, Dynlacht BD and Dutta A(1996)Cyclin- binding motifs are essential for the function of p21Cip1. Mol. Cell. Biol.16:4673—4682.
Choi YB, Tenneti L, Le DA, Ortiz J, Bai G, Chen HS and Lipton SA (2000) Molecular basis of NMDA receptor-coupled ion channel modulation by S-nitrosylation. Nat. Neurosci. 3:15—21.
De Azevedo WF, Leclerc S, Meijer L, Havlicek L, Strnad M and Kim SH(1997)Inhibition of cyclin-dependent kinases by purine analogues: crystal structure of human cdk2 complexed with roscovitine. Eur J Biochem. 243:518-526.
DelaTorre A, Schroeder RA and Kuo PC (1997) Alteration of NF-kappa B p50 DNA binding kinetics by S-nitrosylation. Biochem. Biophys. Res. Commun. 238:703—706.
delaTorre A, Schroeder RA, Bartlett ST and Kuo PC (1998) Differential effects of nitric oxide-mediated S-nitrosylation on p50 and c-jun DNA binding. Surgery 124:137—141.
Devary Y, Gottlieb RA, Lau LF and Karin M (1991) Rapid and preferential activation of the c-jun gene during the mammalian UV response. Mol. Cell. Biol. 11:2804—2811
Duhe RJ, Nielsen MD, Dittman AH, Villacres EC, Choi EJ and Storm DR (1994) Oxidation of critical cysteine residues of type I adenylyl cyclase by o-iodosobenzoate or nitric oxide reversibly inhibits stimulation by calcium and calmodulin. J. Biol. Chem. 269:7290—7296.
Edamatsu H, Gau CL, Nemoto T, Guo L and Tamanoi F(2000)Cdk inhibitors, roscovitine and olomoucine, synergize with farnesyltransferase inhibitor (FTI) to induce efficient apoptosis of human cancer cell lines. Oncogene 19:3059-3068.
Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A and Nagata S (1998) A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 391:43—50
Ezhevsky SA, Nagahara H, Vocero-Akbani AM, Gius DR, Wei MC and Dowdy SF(1997)Hypo-phosphorylation of the retinoblastoma protein (pRb) by cyclin D:Cdk4/6 complexes results in active pRb. Proc. Natl. Acad. Sci. 94:10699—10704.
Finkel T (1999) Signal transduction by reactive oxygen species in non-phagocytic cells. J. Leukocyte Biol. 65:337—340
Galea E, Reis DJ, Fox ES, Xu H and Feinstein DL(1996)CD14 mediate endotoxin induction of nitric oxide synthase in cultured brain glial cells. J. Neuroimmunol. 64:19-28.
Geng Y, Valbracht J and Lotz M (1996) Selective activation of the mitogen-activated protein kinase subgroups c-Jun NH2 terminal kinase and p38 by IL-1 and TNF in human articular chondrocytes. J. Clin. Invest. 98:2425—2430
Geppert TD, Whitehurst CE, Thompson P and Beutler B (1994) Lipopolysaccharide signals activation of tumor necrosis factor biosynthesis through the ras/raf-1/MEK/MAPK pathway. Mol.Med. 1:93—103
Glab N, Labidi B, Qin LX, Trehin C, Bergounioux C and Meijer L.(1994)Olomoucine, an inhibitor of the cdc2/cdk2 kinases activity, blocks plant cells at the G1 to S and G2 to M cell cycle transitions. FEBS Lett.2:207-211.
Gonzalez-Scarano F and Baltuch G (1999) Microglia as mediators of inflammatory and degenerative diseases. Annu. Rev. Neurosci. 22:219—240.
Gopalakrishna R, Chen ZH and Gundimeda U (1993) Nitric oxide and nitric oxide-generating agents induce a reversible inactivation of protein kinase C activity and phorbol ester binding. J. Biol. Chem. 268:27180—27185.
Gordon S (1998) The role of the macrophage in immune regulation. Res. Immunol 149:685—688
Gremo F, Sogos V, Ennas MG, Meloni A, Persichini T, Colasanti M and Lauro GM (1997) Features and functions of human microglia cells. Adv.Exp.Med.Biol 429:79—97.
Gu Y, Turck CW and Morgan DO(1993)Inhibition of CDK2 activity in vivo by an associated 20K regulatory subunit. Nature 366:707—710.
Hambleton J, Weinstein SL, Lem L and DeFranco AL (1996) Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc.Natl Acad Sci. U. S. A. 93:2774—2778
Han IO, Kim KW, Ryu JH and Kim WK(2002)p38 mitogen-activated protein kinase mediates lipopolysaccharide, not interferon-gamma, -induced inducible nitric oxide synthase expression in mouse BV2 microglial cells. Neurosci Lett.325:9-12.
Hannon GJ and Beach D(1994)p15INK4b is a potential effector of TGFβ-induced cell cycle arrest. Nature 371:257—261.
Hanukoglu I, Rapoport R, Weiner L and Sklan D (1993) Electron leakage from the mitochondrial NADPH-adrenodoxin reductase—adrenodoxin- P450scc (cholesterol side chain cleavage) system. Arch. Biochem.Biophys. 305:489—498
Hirai H, Roussel MF, Kato JY, Ashmun RA and Sherr CJ(1995)Novel INK4 proteins, p19 and p18, are specific inhibitors of the cyclin D-dependent kinases CDK4 and CDK6. Mol. Cell. Biol.15:2672—2681.
Kim WG, Mohney RP, Wilson B, Jeohn GH, Liu B and Hong JS (2000) Regional difference in susceptibility to lipopolysaccharide-induced neurotoxicity in the rat brain: role of microglia. J. Neurosci 20:6309-6316.
Kitagawa M, Higashi H, Jung HK, Suzuki-Takahashi I, Ikeda M, Tamai K, Kato J, Segawa K, Yoshida E, Nishimura S and Taya Y(1996)The consensus motif for phosphorylation by cyclin D1-Cdk4 is different from that for phosphorylation by cyclin A/E-Cdk2. EMBO J. 15:7060—7069.
Kopec KK and Carroll RT (1998) Alzheimer''s beta-amyloid peptide 1-42 induces a phagocytic response in murine microglia. J. Neurochem 71: 2123—2131
Lander HM, Hajjar DP, Hempstead BL, Mirza UA, Chait BT, Campbell S and Quilliam LA (1997) A molecular redox switch on p21(ras). Structural basis for the nitric oxide-p21(ras) interaction. J. Biol. Chem. 272:4323—4326.
Lander HM, Ogiste JS, Teng KK and Novogrodsky A (1995) p21ras as a common signaling target of reactive free radicals and cellular redox stress. J. Biol. Chem.270:7017—7020.
Lee JC, Laydon JT, McDonnell PC, Gallagher TF, Kumar S, Green D, McNulty D, Blumenthal MJ, Heys JR and Landvatter SW (1994) A protein kinase involved in the regulation of inflammatory cytokine biosynthesis Nature 372:739—746
Lee MH, Reynisdottir I and Massague J(1995)Cloning of p57Kip2, a cyclin-dependent kinase inhibitor with unique domain structure and tissue distribution. Genes Dev 9:639—649.
Lowenstein CJ, Alley EW, Raval P, Snowman AM, Synder SH, Russell SW and Murphy WJ (1993) Macrophage nitric oxide synthase gene: Two upstream regions mediate induction by interferon-gand lipopolysaccharide. Proc Natl Acad Sci USA 90:9730—9734.
Ludlow JW, Glendening CL, Livingston DM and DeCarprio JA(1993)Specific enzymatic dephosphorylation of the retinoblastoma protein. Mol. Cell. Biol. 13:367—372.
Ludlow JW, Shon J, Pipas JM, Livingston DM and DeCaprio JA(1990)The retinoblastoma susceptibility gene product undergoes cell cycle-dependent dephosphorylation and binding to and release from SV40 large T. Cell 60:387—396.
Lundberg AS and Weinberg RA(1998)Functional inactivation of the retinoblastoma protein requires sequential modification by at least two distinct cyclin-CDK complexes. Mol.Cell. Biol. 18:753—761.
Maccioni RB, Munoz JP and Barbeito L (2001) The molecular bases of Alzheimer’s diseases and other neurodegenerative disorders. Arch Med Res 32:367-381
Mannick JB, Hausladen A, Liu L, Hess DT, Zeng M, Miao QX, Kane LS, Gow AJ and Stamler JS (1999) Fas-induced caspase denitrosylation. Science 284:651—654.
Marletta MA (1994) Nitric oxide synthase: aspects concerning structure and catalysis. Cell 78:927—930.
Matsushime H, Quelle DE, Shurtleff SA, Shibuya M, Sherr CJ and Kato JY(1994)D-type cyclin-dependent kinase activity in mammalian cells. Mol. Cell. Biol. 14:2066—2076.
McConnell BB, Gregory FJ, Stott FJ, Hara E and Peters G(1999)Induced expression of p16INK4a inhibits both CDK4 and CDK2-associated kinase activity by reassortment of cyclin-CDK-inhibitor complexes. Mol. Cell. Biol. 19:1981—1989.
Meda L, Cassatella MA, Szendrei GI, Otvos L Jr, Baron P, Villalba M, Ferrari D and Rossi F (1995) Activation of microglial cells by betaamyloid protein and interferon-gamma. Nature 374:647-650.
Meyerson M and Harlow E(1994)Identification of a G1 kinase activity for cdk6, a novel cyclin D partner. Mol. Cell. Biol.14:2077—2086.
Minghetti L and Levi G (1998) Microglia as effector cells in brain damage and repair: focus on prostanoids and nitric oxide. Prog. Neurobiol 54:99—125.
Munoz-Fernandez MA and Fresno M (1998) The role of tumour necrosis factor, interleukin 6, interferon-gamma and inducible nitric oxide synthase in the development and pathology of the nervous system. Prog. Neurobiol. 56:307—430
Nathan C and Xie QW (1994) Nitric oxide synthases: roles, tolls, and controls. Cell 78:915—918.
Okuno S, Shimizu S, Ito T, Nomura M, Hamada E, Tsujimoto Y and Matsuda H(1998)Bcl-2 prevents caspase-independent cell death. J Biol Chem. 273:34272-34277.
Padmanabhan J, Park DS, Greene LA and Shelanski ML(1999)Role of cell cycle regulatory proteins in cerebellar granule neuron apoptosis. J Neurosci. 19:8747-8756.
Park DS, Farinelli SE and Greene LA (1996) Inhibitors of cyclin- dependent kinases promote survival of post-mitotic neuronally differentiated PC12 cells and sympathetic neurons. J Biol Chem 271:8161—8169.
Park DS, Morris EJ, Greene LA and Geller HM (1997) G1/S cell cycle blockers and inhibitors of cyclin-dependent kinases suppress camptothecin-induced neuronal apoptosis. J. Neurosci.17:1256—1270
Park DS, Morris EJ, Padmanabhan J, Shelanski ML, Geller HM and Greene LA (1998b) Cyclin-dependent kinases participate in death of neurons evoked by DNA-damaging agents. J. Cell Biol 143:457—467
Park DS, Morris EJ, Stefanis L, Troy CM, Shelanski ML, Geller HM and Greene LA (1998a) Multiple pathways of neuronal death induced by DNA-damaging agents, NGF deprivation, and oxidative stress.J. Neurosci. 18:830—840
Park HS, Huh SH, Kim MS, Lee SH and Choi EJ(2000)Nitric oxide negatively regulates c-Jun N-terminal kinase/stress-activated protein kinase by means of S-nitrosylation. Proc Natl Acad Sci U S A. 97:14382-14387.
Park HS, Park E, Kim MS, Ahn K, Kim IY and Choi EJ (2000) Selenite inhibits the c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) through a thiol redox mechanism. J. Biol.Chem. 275:2527—2531.
Parry D, Bates S, Mann DJ and Peters G(1995)Lack of cyclin D-cdk complexes in Rb-negative cells correlates with high levels of p16INK4/MTS1 tumor suppressor gene product. EMBO J. 14:503—511.
Parry D, Mahony D, Wills K and Lees E(1999)Cyclin D-CDK subunit arrangement is dependent on the availability of competing INK4 and p21 class inhibitors. Mol. Cell. Biol.19:1775—1783.
Petrova TV, Akama KT and Van Eldik LJ (1999) Selective modulation of BV-2 microglial activation by prostaglandin E2:differential effects on endotoxin-stimulated cytokine induction. J.Biol Chem.274:28823-28827
Pombo CM, Bonventre JV, Avruch J, Woodgett JR, Kyriakis JM and Force T (1994) The stress-activated protein kinases are major c-Jun amino-terminal kinases activated by ischemia and reperfusion. J. Biol. Chem. 269:26546—26551
Raingeaud J, Gupta S, Rogers JS, Dickens M, Han J, Ulevitch RJ and Davis RJ (1995) Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine. J. Biol. Chem. 270:7420—7426
Russo AA, Jeffrey PD, Patten AK, Massague J and Pavletich NP(1996)Crystal structure of the p27Kip1 cyclin-dependent kinase inhibitor bound to the cyclin A-cdk2 complex. Nature 382: 325—331.
Scherle PA, Jones EA, Favata MF, Daulerio AJ, Covington MB, Nurnberg SA, Magolda RL and Tracks JM (1998) Inhibition of MAP kinase kinase prevents cytokine and prostaglandin E2 production in lipopolysaccharide-stimulated monocytes. J. Immunol. 161:5681—5686
Serrano M, Hannon GJ and Beach D(1993)A new regulatory motif in cell cycle control causing specific inhibition of cyclinD/CDK4. Nature 366:704—707.
Stamler JS (1994) Redox signaling: nitrosylation and related target interactions of nitric oxide. Cell 78:931—936.
Stoll G and Jander S (1999) The role of microglia and macrophages in the pathophysiology of the CNS. Prog. Neurobiol. 58:233—247.
Tabuchi A, Sano K, Oh E, Tsuchiya T and Tsuda M (1994) Modulation of AP-1 activity by nitric oxide (NO) in vitro: NO-mediated modulation of AP-1. FEBS Lett. 351:123—127.
Toyoshima H and Hunter T(1994)p27, a novel inhibitor of G1 cyclin/cdk protein kinase activity, is related to p21. Cell 78:67—74.
Troy CM, Stefanis L, Prochiantz A, Greene LA, Shelanski ML (1996a)
The contrasting roles of ICE family protease and interleukin-1b in apoptosis induced by trophic factor withdrawal and by copper/zinc superoxide dismutase down-regulation. Proc Natl Acad Sci USA 93:5635—5640.
Troy CM, Stefanis L, Greene LA, Shelanski ML (1997) Nedd2 is required for apoptosis after trophic support withdrawal, but not superoxide dismutase (SOD1) down regulation, in sympathetic neurons and PC12 cells. J Neurosci 17:1911—1918.
VVesely J, Havlicek L, Strnad M, Blow JJ, Donella-Deana A, Pinna L, Letham DS, Kato J, Detivaud L and Leclerc S(1994)Inhibition of cyclin-dependent kinases by purine analogues. Eur J Biochem. 224:771-786.
Xie QW, Wishnan R and Nathan C (1993) Promotor of the mouse gene-encoding calcium-independent nitric oxide synthase confers inducibility by interferon-gand bacterial lipopolysaccharide. J Exp Med 177:1779—1784.
Xiong Y, Hannon GJ, Zhang H, Casso D, Kobayashi R and Beach D (1993)p21 is a universal inhibitor of cyclin kinases. Nature 366:701—704.
Zhou X, Gordon SA, Kim YM, Hoffman RA, Chen Y, Zhang XR, Simmons RL and Ford HR(2000)Nitric oxide induces thymocyte apoptosis via a caspase-1-dependent mechanism. J Immunol. 165:1252-1258.
Zielasek J and Hartung HP (1996) Molecular mechanisms of microglial activation. Adv. Neuroimmunol 6:191—222
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
1. 樟芝發酵液之抗發炎及其誘導癌細胞凋亡機制之探討
2. 紫草素及其衍生物對內毒素致發炎反應之機制探討
3. 台灣五葉松松針之抗氧化及其抗發炎效果
4. 超氧陰離子與一氧化氮引發粒線體功能失調在脊髓受傷後脊髓神經元發生細胞凋亡所扮演的角色之探討
5. 氧化壓力誘導培養之大白鼠大腦皮質細胞凋亡:神經細胞與神經膠質細胞之不同反應性
6. Mechanismsinvolvedinanticarcinogenicpropertiesofrosemaryphytopolyphenols
7. 探討利用細胞週期抑制劑olomoucine抑制小神經膠細胞發炎反應及一氧化氮所誘發細胞凋亡之研究
8. 探討由LPS刺激巨噬細胞生成ROS媒介MBT-2致死之機制
9. 澳洲茶樹精油水溶性成份抗發炎之機制探討
10. Phenethylisothiocyanate(PEITC)與Benzylisothiocyanate(BITC)經由氧自由基(ROS)與粒線體路徑誘導人類骨肉瘤細胞U-2OS細胞凋亡
11. 顆粒性白血球群落刺激因子可刺激Akt/GSK3β/NFκB的訊息傳遞以抑制內毒素引起之過渡活化的微膠細胞
12. 抑制肝醣合成酶激酶-3改善内毒血症引起的急性腎衰竭:小鼠模式的研究
13. 登革熱二型病毒核心蛋白於人類大主動脈內皮細胞所造成一氧化氮活性及細胞凋亡之影響
14. 在內毒素刺激下顆粒性白血球-群落刺激因子對神經及微膠細胞的作用
15. 兩種腸道上皮細胞株在常態生長與細菌內毒素刺激下NFkappaB活化及細胞凋亡現象關聯性之探討
 
系統版面圖檔 系統版面圖檔