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研究生:賴國誌
研究生(外文):Kuochih-Lai
論文名稱:姬蕨抗癌成分之研究
論文名稱(外文):Studies on the Cytotoxic Principles from Hypolepis punctata
指導教授:徐鳳麟徐鳳麟引用關係
學位類別:碩士
校院名稱:臺北醫學大學
系所名稱:生藥學研究所
學門:醫藥衛生學門
學類:藥學學類
論文種類:學術論文
論文出版年:2003
畢業學年度:91
語文別:中文
論文頁數:112
中文關鍵詞:姬 蕨Pterosin+-pinoresinol-β-D-glucoside
外文關鍵詞:Hypolepis punctataPterosin+-pinoresinol-β-D-glucoside
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為期由天然物中開發具臨床價值之抗癌新藥,本論文以初步篩選顯示抗癌活性之植物「姬蕨」,配合活性實驗,進行活性成分分離與結構決定之研究。姬蕨全草鮮品之甲醇萃取物,經以n-Hexane, Ethyl acetate及H2O分配萃取,再藉由各種層析法,共分離出五個化合物,根據其物理數據及光譜儀器分析鑑定,其結構分別確定為Pterosin Z(1), Pterosin I(2), Pterosin A(3), Pterosin D(4)及(+)-pinoresinol-β-D-glucoside(5)。而(+)-pinoresinol-β-D-glucoside則首次於姬蕨中被分離出。另外乙酸乙酯層經管柱層析後,經初步藥理活性篩選,確定對胃癌(NUGC-3)及鼻咽癌(HONE-1)細胞株有明顯細胞毒性作用,而該活性部分分離出Pterosin D(4)。經藥理活性測試結果,首次證實Pterosin Z(1)及Pterosin I(2)對Hep G2肝癌細胞株有細胞毒性,其IC50分別為55.1μg/ml及32.8 μg/ml。
For developing to existed the clinical value using in the new anticancer drugs development. This research is the preliminary step to sieve cytotoxic exhibiter out from Hypolepis punctata (Thunb.) Mett. The fresh full plants of Hypolepis punctata (Thunb.) Mett., were extracted with methanol, to afford crude extracted, which was partitioned between n-Hexane, Ethyl acetate and H2O. These fraction were subject to various chromatography and preliminary screen test cytotoxic activity. Five compounds were isolated and purified. The structures of these compound were determined based on the analysis of their physical property and spectral studies, they are Pterosin Z(1), Pterosin I(2), Pterosin A(3), Pterosin D(4) and(+)-pinoresinol-β-D-glucoside(5). Among them, Fraction 5-1 of Ethyl acetate layer showed cytotoxic effects to NUGC-3 and HONE-1 cells. Moreover, it is note worthy that (+)-pinoresinol-β-D-glucoside(5) was isolated from Hypolepis punctata (Thunb.) Mett. for the first time. Pterosin Z(1) and Pterosin I(2) exhibited cytotoxic effects on the Hep G2 cells, which IC50 are 55.1μg/ml and 32.8μg/ml.
目錄 I
圖表 III
中文摘要 VII
英文摘要 VIII
基本架構 IX
名詞縮寫 X
緒論 1
一、 研究背景及目的 1
二、 台灣蕨類天然物化學研究 2
三、 姬蕨之相關研究 14
第一章 抽提與分離 17
第二章 化合物之構造決定 20
一、 Sesquiterpenes 20
1. HP-1 Pterosin Z (1) 20
2. HP-2 Pterosin I (2) 32
3. HP-3 Pterosin A(3) 40
4. HP-4 Pterosin D(4) 45
二、 Lignans 51
HP-5 (+)-pinoresinol-β-D-glucoside(5) 51
結論 73
一、 姬蕨化合物之探索部分 73
二、 藥理之探索部分 77
實驗部分 79
一、 儀器與材料 79
二、 層析法 80
三、 相關實驗 82
第一節 抽提與分離 82
第二節 相關成分物理性質 84
參考文獻 90
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