臺灣博碩士論文加值系統

製備出微粒子，此粒子粒徑範圍在5.6~9.8 μm，整體粒徑＜10μm，使用此粒子當作藥物 ( 5-FU )之載體 ( carrier )，此載體本身具有高孔隙性 ( 孔隙度可達到55 % )。然後將載體置入一已含藥之溶液中，此溶液為不同濃度 ( w/w % )之poly acrylic acid ( PAA ) ( 3.5%、0.35%與0.035% )與Chitosan ( 0.3%、0.2%與0.1% )，其比例為載體:藥物:溶液= 0.3g : 0.01g : 10 ml，以微膠囊( microcapsule )之型式包覆藥物/載體。然後在不同pH值 ( pH 3、pH 4.5、pH 6、pH 7.4、pH 8.4 )作藥物釋放之測試。利用Mt / M = ktn之方程式，確定藥物釋放的量與時間之關係，得到n值在0.03-0.25的範圍內，並與Fickian diffusion的結果比較之，說明我們的釋放為一non-Fickian。最後，模擬藥物在人體內部 ( 口腔 ( 0-5 min )-胃( 5 min-2 h )-小腸 ( 2-5 h )-大腸 ( 5-24 h ) )不同pH 環境之釋放情形，個別累積釋放量為口腔:1.12×103 mg/g；胃:1.39×103 mg/g；小腸:2.06×103 mg/g；大腸:3.05×103 mg/g。

目錄
第一章 序論…………………………………………………………... 1
1-1前言……………………………………………………………. 1
1-2研究動機與目標………………………………………………. 2
1-3 5-FU之介紹…………………………………………………... 3
1-4 pH敏感性 ( pH-sensitivity ) 高分子之介紹 ( PAA )………. 4
1-5 Chitin與Chitosan之介紹……………………………………. 6
第二章 文獻回顧…………………………………………………….. 7
第三章 理論…………………………………………………………. 11
3-1 微粒子………………………………………………………. 11
3-2 分散聚合法…………………………………………………. 12
3-3 粒種乳化聚合法……………………………………………. 12
3-4 藥物釋放……………………………………………………. 13
3-5 藥物之吸收…………………………………………………. 16
第四章 實驗部分…………………………………………………… 17
4-1 藥品…………………………………………………………. 17
4-2 儀器設備……………………………………………………. 18
4-3 實驗步驟………………………………………………….… 20
4-3-1 Polystyrene粒種製備流程……………………………... 20
4-3-2粒子之製備與性質檢測……………………………….. 20
4-3-3 粒子吸水測試…………………………………………. 22
4-3-4粒子 + PAA + 5-FU……………………………………. 22
4-3-5粒子 + PAA + 5-FU + Chitosan……………………….. 23
4-3-6粒子 + Chitosan + 5-FU……………………………….. 23
4-3-7 粒子 + Chitosan + 5-FU + PAA………………………. 23
4-3-8 藥物釋放………………………………………………. 24
4-3-8-1 Phosphate buffered saline ( PBS )溶液調配……… 24
4-3-8-2 5-FU之UV圖譜與減量線………………………. 24
4-3-8-3釋放步驟………………………………………….. 25
第五章 結果討論…………………………………………………... 27
5-1 FT/IR………………………………………………………... 27
5-2 孔隙度……………………………………………………… 27
5-3 粒子粒徑、分佈與形狀…………………………………… 28
5-4 TGA…………………………………………………………. 29
5-5 DSC…………………………………………………………. 29
5-6 粒子澎潤比曲線…………………………………………… 30
5-7 藥物釋放…………………………………………………… 31
第六章 結論………………………………………………………... 37
參考文獻……………………………………………………………. 87

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