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研究生:蘇加新
研究生(外文):Chia-Shin Su
論文名稱:三黃瀉心湯品質管制及其活性成份藥物動力學研究
論文名稱(外文):Quality control and active components pharmacokinetics of San-huang-xie-xin-tang
指導教授:蔡義弘蔡義弘引用關係
指導教授(外文):Yi-Hung Tsai
學位類別:碩士
校院名稱:高雄醫學大學
系所名稱:藥學研究所碩士班
學門:醫藥衛生學門
學類:藥學學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:98
中文關鍵詞:大黃黃連黃芩三黃藥動學分析交互作用中藥
外文關鍵詞:baicalinanalysissennosideherbpharmacokineticSan-huang-xie-xin-tang
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本實驗目的為利用HPLC分析方法,針對三黃瀉心湯的組成生藥與各種製劑,進行13種指標成份的定量與指紋圖譜的建立,同時評估其體外交互作用,以達到中藥品質管制的功能,並進而分析三黃瀉心湯活性成份的藥物動力學。
為了了解三黃瀉心湯各個成份的生藥來源與單方,複方藥物動力學的差異,我們進一步製備單一生藥空白湯劑與三黃瀉心湯離心冷凍乾燥粉末,並進行分析。
利用HPLC分析1個檢品的時間為60分鐘,在分析組成生藥與各種製劑活性成份時,發現黃芩的baicalin含量最高,而且黃芩的指紋圖譜與三黃瀉心湯的指紋圖譜最為類似,接著我們也建立了三黃瀉心湯可以說明各個活性成份生藥來源的指紋圖譜,在體外交互作用方面,則發現黃連分別與大黃或黃芩煎煮時,會產生沉澱而使活性成份含量降低。
在藥物動力學實驗方面,我們分別在大鼠腹腔注射單一生藥與三黃瀉心湯離心冷凍乾燥製劑,利用本實驗分析方法可以成功分析到6個活性成份,針對活性成份的血中濃度分析發現,複方的活性成份和單方相比,複方的活性成份會有延緩釋出的情形。
所以從本實驗發現黃芩可能為三黃瀉心湯的君藥,而黃連具有相須,相使的功能。大黃則扮演臣藥的角色。
The purpose of this study was to use HPLC analysis method to qualify 13 compounds in herbs and products of San-huang-xie-xin-tang and to build the fingerprints of San-huang-xie-xin-tang. Then we try to evaluate the interaction in vitro. Such procedures can make us evaluate the quality control and the active components pharmacokinetics of San-huang-xie-xin-tang.
We made single herb powder, single herb blank powder, and San
-huang-xie-xin-tang standard powder because we would like to know the herb origins of all compounds and the difference of pharmacokinetics in single herb and San-huang-xie-xin-tang.
In the study, one sample needs 60 minutes to analyze. We found baicalin percentage in Scutellariae radix was the most when we analyzed all samples. The fingerprint of Scutellariae radix was as the same as fingerprint of San-huang-xie-xin-tang almost. We also built the San-huang
-xie-xin-tang fingerprint. In interaction aspect, Coptidis rhizome with Rhei rhizome and Coptidis rhizome with Scutellariae radix made precipitate when decocting together.
We could analyze 6 compounds in rat plasma when we I.P. San
-huang-xie-xin-tang and single herb. According to the results of analysis in vivo, We found combination of herbs could make compound release and metabolize more slowly.
目錄
頁次
壹、 摘要
中文摘要 1
英文摘要 2

貳、 緒論
一、 研究背景 3
二、 中藥品質管制之基本概述 4
三、 中藥藥物動力學之基本概述 5
四、 三黃瀉心湯之基本概述 6
五、 三黃瀉心湯各藥材與成份之基本概述 7
六、 三黃瀉心湯各藥材活性成份之分析研究 14
七、 三黃瀉心湯活性成份藥動學之研究 17

參、 實驗目的 21

肆、 材料與儀器設備
一、 材料 22
二、 儀器 23
伍、 實驗方法
一、 HPLC分析條件的確立 24
二、 體外檢量線的製備 25
三、 大黃,黃連,與黃芩各藥材的活性成份 25
含量分析
四、 三黃瀉心湯的製備 26
五、 三黃瀉心湯活性成份的含量分析 27
六、 體內實驗分析方法的建立 28
七、 三黃瀉心湯體內藥動學實驗 28
八、 三黃瀉心湯藥動學資料分析 29

陸、 結果與討論
一、 體外實驗分析方法 30
二、 大黃,黃連與黃芩藥材分析結果 43
三、 三黃瀉心湯自製品分析結果 47
四、 體內實驗分析方法 64
五、 三黃瀉心湯活性成份的藥物動力學 71
柒、 結論 94
捌、 參考文獻 95
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