本實驗目的為利用HPLC分析方法，針對三黃瀉心湯的組成生藥與各種製劑，進行13種指標成份的定量與指紋圖譜的建立，同時評估其體外交互作用，以達到中藥品質管制的功能，並進而分析三黃瀉心湯活性成份的藥物動力學。
為了了解三黃瀉心湯各個成份的生藥來源與單方，複方藥物動力學的差異，我們進一步製備單一生藥空白湯劑與三黃瀉心湯離心冷凍乾燥粉末，並進行分析。
利用HPLC分析1個檢品的時間為60分鐘，在分析組成生藥與各種製劑活性成份時，發現黃芩的baicalin含量最高，而且黃芩的指紋圖譜與三黃瀉心湯的指紋圖譜最為類似，接著我們也建立了三黃瀉心湯可以說明各個活性成份生藥來源的指紋圖譜，在體外交互作用方面，則發現黃連分別與大黃或黃芩煎煮時，會產生沉澱而使活性成份含量降低。
在藥物動力學實驗方面，我們分別在大鼠腹腔注射單一生藥與三黃瀉心湯離心冷凍乾燥製劑，利用本實驗分析方法可以成功分析到6個活性成份，針對活性成份的血中濃度分析發現，複方的活性成份和單方相比，複方的活性成份會有延緩釋出的情形。
所以從本實驗發現黃芩可能為三黃瀉心湯的君藥，而黃連具有相須，相使的功能。大黃則扮演臣藥的角色。

The purpose of this study was to use HPLC analysis method to qualify 13 compounds in herbs and products of San-huang-xie-xin-tang and to build the fingerprints of San-huang-xie-xin-tang. Then we try to evaluate the interaction in vitro. Such procedures can make us evaluate the quality control and the active components pharmacokinetics of San-huang-xie-xin-tang.
We made single herb powder, single herb blank powder, and San
-huang-xie-xin-tang standard powder because we would like to know the herb origins of all compounds and the difference of pharmacokinetics in single herb and San-huang-xie-xin-tang.
In the study, one sample needs 60 minutes to analyze. We found baicalin percentage in Scutellariae radix was the most when we analyzed all samples. The fingerprint of Scutellariae radix was as the same as fingerprint of San-huang-xie-xin-tang almost. We also built the San-huang
-xie-xin-tang fingerprint. In interaction aspect, Coptidis rhizome with Rhei rhizome and Coptidis rhizome with Scutellariae radix made precipitate when decocting together.
We could analyze 6 compounds in rat plasma when we I.P. San
-huang-xie-xin-tang and single herb. According to the results of analysis in vivo, We found combination of herbs could make compound release and metabolize more slowly.

目錄
頁次
壹、 摘要
中文摘要 1
英文摘要 2

貳、 緒論
一、 研究背景 3
二、 中藥品質管制之基本概述 4
三、 中藥藥物動力學之基本概述 5
四、 三黃瀉心湯之基本概述 6
五、 三黃瀉心湯各藥材與成份之基本概述 7
六、 三黃瀉心湯各藥材活性成份之分析研究 14
七、 三黃瀉心湯活性成份藥動學之研究 17

參、 實驗目的 21

肆、 材料與儀器設備
一、 材料 22
二、 儀器 23
伍、 實驗方法
一、 HPLC分析條件的確立 24
二、 體外檢量線的製備 25
三、 大黃，黃連，與黃芩各藥材的活性成份 25
含量分析
四、 三黃瀉心湯的製備 26
五、 三黃瀉心湯活性成份的含量分析 27
六、 體內實驗分析方法的建立 28
七、 三黃瀉心湯體內藥動學實驗 28
八、 三黃瀉心湯藥動學資料分析 29

陸、 結果與討論
一、 體外實驗分析方法 30
二、 大黃，黃連與黃芩藥材分析結果 43
三、 三黃瀉心湯自製品分析結果 47
四、 體內實驗分析方法 64
五、 三黃瀉心湯活性成份的藥物動力學 71
柒、 結論 94
捌、 參考文獻 95

Chang, H. M.Pharmacology and applications of Chinese Materia Medica., 1987; 2:1022-1028
Chen, C. M and Chang, H. C., Determination of berberine in plasma, urine, and bile by high-performance liquid chromatography. J. Chromatogr. B, 1995; 665: 17-123
Chen, X. Y., Wang, H. G., Du, Y., Zhong, D., Quantitation of the flavonoid wogonin and its major metabokite wogonin-7β-D-glucuronide in rat plasma by liquid chromatography-tandem mass spectrometry. J. Chromatogr. B, 2002; 775: 169-178
De Witte, P., Metabolism and pharmacokinetics of anthranoid. Pharmacology 1 (Suppl. 47), 1993:86-97
Kazuhiko, S., Toshiyuki, O., Tsuguchika, Y., Rapid and simple determination of sennoside A and B in Rhei Rhizoma by ion-pair high–performance–liquid　-chromatography. J. Chromatogr. , 1987;403:253-261
Kimura, Y., Kubo, M.,Tani, T., Arichi, S., Okuda, H.,Effect on lipidperoxidation in rat liver. Chem. Pharm. Bull., 1981;29:2610-2617
Lai, M. Y., Hsiu, S. L., Tsai, S. Y., Hou, Y. C., Lee P. D., Comparison of metabolic pharmacokinetics of baicalin and baicalein in rats. J. Pharm. Pharmacol., 2003:205-209
Lee, J. H., Kim, J. M., Kim, C. S., Pharmacokinetic analysis of rhein in Rheum undulatum L. J. Ethnopharmcol., 2003;84:5-9
Liang, J. W., Hsiu, S. L., Wu, P. P., Chao, P. D. L., Emodin pharmacokinetics in rabbits. Planta Med., 1995;65:406-408
Lin, S. J., Tseng, H. H., Wen, K. C., Suen, T. T., Determination of gentiopicroside, mangiferin, palmatine, berberine, baicalin, wogonin, and glycyrrhizin in the traditional Chinese medicinal preparation Sann-joong –kuey -jian-tang by high-performance liquid chromatography. J. Chromatogr. A, 1996; 730:17-23
Maity, T. K., Mandal, S. C., Bhakta, T., Pal, M., Saha, B. P., Metabolism of 1,8-dihydroxy 3-hydroxy methyl anthraquinone(aloe-emodin) isolated from the leaves of Cassia tora in Albino rats. Phytother. Res., 2001;15:459-460
Merck index, twelfth edition
Nicolas, P., Tod, M., Padoin, C., Petitjean, O., Clinical Pharmacokinetics of diacerein. Clin. Pharmacokinet., 1998;35:347-359
Okamura, N., Miki, H., Ishida, S., Ono, H., Yano, A., Tanaka, T., Ono, Y., Yagi, A., simultaneous determination of baicalin, wogonoside, baicalein, wogonin, berberine, coptisine, palmatine, jateorrhizine and glycyrrhizin in kampo medicines by ion-pair high-performance liquid chromatography. Biol. Pharm. Bull.,1999;22:1015-1021
Sagara, K., Ito, Y., Oshima, T., Misaki, T., Murayama, H., Simultaneous determination of baicalein, wogonin, oroxylin-A and their glucuronides in Scutellariae radix by ion-pair high-performance liquid chromatography. J. Chromatogr., 1985;328:289-297
Spence, C. M., Wilde, M. I., Diacerein. Drugs. 1997;53:98-106
Springolo, V. and Coppi, G., Simple method for the determination of rhein in biological fluids by high-performance liquid chromatography. J. Chromatogr. B., 1988;428:173-177
Teruaki, A., Kiyoshi, K., Erika, Y., Kazuhisa, I., Yasuharu, M., Yoko, W., Yoko, S., Kyoichi, K.J., Baicalin, the predominant flavone glucuronide of Scutellariae Radix, is absorbed from the rat gastrointestinal tract as the aglycone and restored to its original form. J. Pharm. Pharmacol., 2000;52:1563-1568
Tsai, T. H., Chou, C. J., Tsai, T. R., Chen, C. F., Determination of wogonin in rat plasma by liquid chromatography and its pharmacokinetic application. Planta. Med., 1996;62:263-266
Wu, J. M., Chen, D. W., Zhang, R.H. Study on the bioavailability of baicalin -phospholipid complex by using HPLC. Biomed. Chromatogr., 1999; 13: 493 -495
Yagi, T., Miyawaki, Y., Nishikawa, A., Horiyama, S., Yamauchi, K., Kuwano, S., J. Pharm. Pharmacol., 1990;42:542-545
Tetsuo, M., Kazuhiko, S., Mitsuharu, O., Sadao, K., Toshiyuki, O., Hiroshi, Y., Simultaneous determination of berberine, palmatine, coptisine in crude drug and oriental pharmaceutical preparation by ion-pair high-performance liquid chromatography. Chem. Pharm. Bull., 1982;30:354-357
Xie, L. H., Wang, X., Basnet, P., Matsunaga, N., Yamagi, S., Evaluation of variation acetoside and three major flavonoids in wild and cultivated Scutellaria baicalensis roots by micellar electrokinetic chromatography. Chem. Pharm. Bull., 2002;50:896-899
Yukio, O., Yohei, O., and Kunio, T., Hugh-performance liquid chromatographic separation of rhubarb constituents. J. Chromatogr. , 1986;360:303-306
Zeng, X. J. and Zeng, X. H., Relationship between the clinical effects of berberine on severe congestive heart failure and its concentration in plasma studied by HPLC. Biomed. Chromatogr., 1999;13:442-444
Zeng, X. H., Zeng, X. J., Lee, Y. Y., Huang, Y. H., J. Heart. Failure., 1997;4:66
矢數道明，1985，�c臨床應用漢方處方解說�c，正言出版社，28-29。
行政院衛生署中醫藥委員會，1999，�c中藥材品質管制-組織型態學鑑定�c。
行政院衛生署中醫藥委員會，1998，中醫藥年報3，1-2。
吳堅東，1993，�c板藍根注射液在家兔體內藥物動力學的評估�c，中成藥15（3），5。
張志榮、胡曉穎、蔣大義，�c銀黃劑中黃芩在家兔體內的代謝動力學研究�c，藥物分析學雜誌18（6），1。
李丹、肖東睿、許磊等，2001，�c中藥藥物動力學研究概況�c，時珍國醫國藥12，9 。
韓超、潘競鏘、劉惠純、李鎧洁、杜洁煒，2000，�c瀉心湯對正常和多種糖尿病模型的降血糖作用�c，中國實驗方劑學雜誌6，33-35。
成川一郎，1992，�c漢方製劑品質相關研究�c，家庭藥研究11，60-64。
後藤實，1967，日藥誌。
中藥藥理及應用，1984，715-731。
顏焜熒，1970，�c常用中藥之藥理�c，國立中國醫藥研究所，56-67。
林宗旦、林宗平、林景彬等，1980，�c中藥藥理學�c，國立編譯館，163-173。