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研究生:江宜錚
論文名稱:液相層析質譜術於中藥複方當歸拈痛湯與消風散中之五種指標成分之偵測
論文名稱(外文):Determination of five marker compounds in the traditional Chinese medicinal preparation Dang-guei-nian-tong-tang and Shiau-feng-saan by LC/MS/MS
指導教授:鐘婷婷鐘婷婷引用關係
學位類別:碩士
校院名稱:國立中興大學
系所名稱:化學系
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:51
中文關鍵詞:液相層析質譜術苦蔘當歸知母甘草
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本研究是探討利用液相層析質譜儀之電灑游離法正離子模式,來分離偵測二種複方中四種藥材:苦蔘、當歸、知母、甘草中之其指標成分苦蔘鹼、氧化苦蔘鹼、阿魏酸、芒果苷、甘草甜素的定量分析。
研究中實驗所得結果為,苦蔘鹼的線性範圍為50-1500 ng/ml,線性相關係數(R2)為 0.9987,偵測極限為20 ng/ml,氧化苦蔘鹼的線性範圍為50-1500 ng/ml,線性相關係數(R2)為 0.9987,偵測極限為20 ng/ml,阿魏酸的線性範圍為50-1500 ng/ml,線性相關係數(R2)為 0.9977,偵測極限為25 ng/ml,芒果苷的線性範圍為50-2000 ng/ml,線性相關係數(R2)為 0.9975,偵測極限為25 ng/ml,甘草甜素的線性範圍為75-2500 ng/ml,線性相關係數(R2)為 0.9971,偵測極限為50 ng/ml。在複方消風散之含量測定方面,苦蔘鹼的含量分佈由198.5至503.6 g/g,氧化苦蔘鹼的含量分佈由84.6至315.8 g/g,阿魏酸的含量分佈由80.8至217.9 g/g,芒果苷的含量分佈由97.4至113.8 g/g,甘草甜素的含量分佈由75.2至109.4 g/g;當歸拈痛湯的含量測定方面,苦蔘鹼的含量分佈由103.7至215.2 g/g,氧化苦蔘鹼的含量分佈由81.9至127.3 g/g,阿魏酸的含量分佈由79.6至90.1 g/g,芒果苷的含量分佈由77.4至93.7 g/g,甘草甜素的含量分佈由137.5至180.6 g/g。

謝誌 …………………………………………………………i
中文摘要 ..................................................................................ii
目錄 ………………………………...……………………….iii
圖次 ………………………………...……………………….v
表次 …………………………………………………………viii
參考文獻 ……………………………..…………………......52
前言 ………………………………………………………… 1
中文部分
第一章緒論
一、中藥單方之簡介 ………………………………………1
二、五種指標成份之簡介…………………………………..4
三、複方消風散與當歸拈痛湯的簡介……………………..8
四、研究動機………………………………………………..10
第二章實驗與分析方法
一、 標準樣品部分…………………………………………11
二、樣品的置備與萃取……………………………………..11
三、 儀器與設備……………………………………………12
四、 實驗藥品………………………………………………13
五、液相層析質譜儀之實驗部分…………………………..14
(一) 母離子的偵測………………………………………….14
(二) 斷裂離子分析………………………………………….15
(三) 定量離子之掃描模式………………………………….15
(四) 內標的選擇與分析…………………………………….16
(五) 流動注入分析與質譜條件找尋……………………….16
(六) 層析條件的探討……………………………………….17
(七) 質譜儀參數之找尋…………………………………….17
(八) 層析條件總覽………………………………………….24
(九) 質譜條件總覽………………………………………….25
第三章結果與討論
一、電灑游離法液相層析質譜術的分析…………………..29
[1] 不同添加劑對電灑游離法的影響………………………29
[2] 五種指標成份的質譜特性……………………………....30
二、以LC-ESI-MS/MS分析五種指標成分之檢量線測定..42
三、以LC-ESI-MS/MS分析五種指標成分之準確度……..47
四、真實樣品的測定………………………………………..48
第四章結論

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