跳到主要內容

臺灣博碩士論文加值系統

(44.200.77.92) 您好!臺灣時間:2024/02/27 05:05
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

: 
twitterline
研究生:梁書蓉
研究生(外文):liang Shu-jung
論文名稱:台灣母馬生殖道之菌相調查
論文名稱(外文):The survey of the bacterial flora in the genital tract of mares in Taiwan
指導教授:陳鵬文陳鵬文引用關係茅繼良
學位類別:碩士
校院名稱:國立中興大學
系所名稱:獸醫學系
學門:獸醫學門
學類:獸醫學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
中文關鍵詞:菌相母馬調查
外文關鍵詞:bacterial floramaresurvey
相關次數:
  • 被引用被引用:2
  • 點閱點閱:297
  • 評分評分:
  • 下載下載:14
  • 收藏至我的研究室書目清單書目收藏:0
本實驗調查台灣母馬生殖道之菌相,自91年6月至92年7月間,從飼養於台灣中南部四馬場,共30匹健康母馬進行生殖道採樣,細菌培養環境分為好氧 (aerobes)、嗜二氧化碳 (capnophiles) 及厭氧 (anaerobes) 培養,以GFB-14E電腦密碼細菌鑑定系統、Biolog MicroStation電腦密碼自動鑑定系統 (Biolog, Hayward, California, USA)及核醣體16S核醣核酸和其基因 (16S rDNA) 序列定序進行菌種鑑定。總共分離出240株菌,其中子宮內膜、前庭、陰蒂窩、陰蒂竇部位所分離出的細菌數量分別為4、35、90及111株菌種,細菌分離率分別為10.0% (3/30)、66.7% (20/30)、100% (30/30) 及100% (30/30),臨床上健康的母馬仍可由其外生殖道分離出病原菌,包括由十二匹母馬的陰蒂竇、陰蒂窩及前庭及子宮中分離出Escherichia coli [40.0%(12/30)] ,四匹母馬的陰蒂竇、陰蒂窩及前庭分離出Streptococcus zooepidemicus [13.3%(4/30)],一匹無生產紀錄之母馬的陰蒂窩分離出Pseudomonas aeruginosa [3.3%(1/30)] 及由一匹無生產紀錄之母馬的陰蒂竇分離出Klebsiella pneumoniae [3.3%(1/30)]等可能造成子宮內膜炎之病原菌,這些病原在繁殖季節時可能藉由交配而傳播。馬傳染性子宮炎 (contagious equine metritis;CEM) 的病原菌Taylorella equigenitalis皆沒有被分離出。本實驗結果顯示,由有生產記錄的年老母馬子宮中可分離出細菌,前庭可分離出的細菌數也較無生產記錄的母馬較多(21株菌/10匹 vs. 7株菌/6匹)。至於陰蒂部分的細菌數量則和年齡及生產記錄組間沒有差異(101株菌/16匹 vs. 60株菌/8匹)。潛在子宮內膜炎病原菌在外生殖道存在的比例也沒有組別間的差異(7/16, 44 % vs.4/8, 50 %)。本實驗結果顯示,台灣中南部地區健康母馬的生殖道菌相中,其外生殖道存在著潛在的病原菌,故於馬匹配種時應特別注意性病的防治。
This study investigated the bacteria flora in the genital tract of mares in Taiwan. From June 2001 to July 2002, thirty healthy mares in Middle and Southern Taiwan were used for this investigation. Swab sampling of uterus, vestibule, clitoral fossa and clitoral sinus for bacteriological culture were performed on the 30 clinically normal mares during their diestrus period. The swab specimens were inoculated in different incubation atmospheres, including aerobic, capnophilic and anaerobic conditions, respectively. The isolates were purified and identified by methods of GFB-14E, Biolog MicroStation and 16S rDNA sequencing. The result indicated that a total of 240 strains of bacteria were isolated and identified, including 4, 30, 90 and 111 strains from the 4 collected sites, respectively. The isolated rate were 10.0% (3/30)、66.7% (20/30)、100% (30/30) and 100% (30/30) from endometrial, vestibule, clitoral fossa and clitoral sinus, respectively. Pseudomonas aeruginosa (3.3%), Klebsiella pneumoniae (3.3%), Streptococcus zooepidemicus (13.3%), and Escherichia coli (40.0%), which may possibly reduce endometritis, were isolated from the genital tract of clinically health mares. Taylorella equigenitalis was not found in this investigation. There were more bacteria isolated from the uterus in the uterus in the older and multiparous mares. The vestibule harbored more bacteria in the multipara than in those without breeding record (21 strains/10 mares vs. 7 strains /6 mares). The amount of clitoral bacteria has no difference between age and breeding record (101 strains /16 mares vs. 60 strains /8 mares). The isolated rate of potential uterine pathogens have no difference between age and breeding record (7/16, 44 % vs.4/8, 50 %). These results from this investigation in mares in Middle and Southern Taiwan demonstrated that the outer reproductive sites in mares supported the growth of organisms, including potential uterine pathogens. Thus, it is important to prevent transmitting the venereal disease of horses in the breeding season.
表次……………….…………………………………………………………Ⅱ
圖次 …………………………………………………………………………Ⅱ
中文摘要 ……………………………………………………………………Ⅲ
英文摘要 ……………………………………………………………………Ⅴ
第一章 緒言 ….…………………………………..…………………..1
第二章 文獻探討…………………………………………………………2
一、 生殖道常在菌相 ……………………………………………….2
二、 子宮內膜炎的病原菌 …………………………………….....3
三、 子宮防禦機制 ………………………………………………….9
四、 生殖道採樣方法 ………………………………………………15
五、 細菌分類方法 …………………………………………………17
六、 微生物自動鑑定系統 …………………………………………20
第三章 實驗材料與方法 ………………………………………………22
一、 實驗動物 ………………………………………………………22
二、 採樣方法 ………………………………………………………22
三、 菌種分離 ………………………………………………………23
四、 菌種鑑定 ………………………………………………………23
五、 菌種保存 ………………………………………………………27
第四章 結果 ……………………………………………………………28
一、 細菌鑑定 ………………………………………………………28
二、 菌相調查 ………………………………………………………31
第五章 討論 ……………………………………………………………44
一、 細菌鑑定 ………………………………………………………44
二、 菌相調查 ………………………………………………………45
參考文獻 ………………………………………………………………….49
1. 王昭閔。第三章 材料及方法。引自:好氣式堆肥處理之菌相調查及嗜高溫菌之分類,國立中興大學獸醫微生物學研究所碩士論文,台中。27-29,2001。
2. 林哲祺。染色法。引自:獸醫臨床細菌鑑定圖譜,國立中興大學附設家畜醫院編著。藝軒,台北,1-24,1984。
3. 鄭孝全。第五章 討論。引自;犬正常齒齦與牙周病齒齦和齒時之菌相調查,國立中興大學獸醫學系碩士論文,台中。103-112,2002。
4. Allen WE, Pycock JF. Current views on the pathogenesis of bacterial endometritis in mares. Vet Rec 125: 298-301, 1989.
5. Asbury AC, Lyle SK. Infectious causes of infertility. In: Angus OM, James LV. ed. Equine reproduction. Lea & Febiger, Philadelphia, London, 381-389, 1993.
6. Asbury AC, Schultz KT, Klesius PH, Foster GW, Washburn SM. Factors affecting phagocytosis of bacteria by neutrophils in the mare's uterus. J. Reprod. Fert., Suppl 32: 151-159,1982.
7. Blanchard TL, Et al. Comparison of two techniques for obtaining endomrtrial bacteriologic culture in the mare. Theriogenology 16: 85-93, 1981.
8. Blanchard TL, Varner DD, Schumacher J. Uterine defense mechanisms in the mares. In: Blanchard TL, Varner DD, Schumacher J ed. Manual of equine reproduction. Mosby-year book, college station, Taxas, 47-58, 1998.
9. Bleumink-Pluym NMC, Weerdler MEB, Houwers DJ, Parlevliet JM, Colnebrander B, Zeijst BAM. Development and evaluation of PCR test for detection of Taylorella equigenitalis. J Clin Microbiol 32:893-896, 1994.
10. Boyd EH, Allen WE. Absorption of neomycin from the equine uterus: effect of bacterial and chemical endometritis. Vet Rec 122: 37-39, 1988.
11. Chanter N, Vigano F, Collin NC, Mumford JA. Use of a PCR assay for Taylorella equigenitalis applied to samples from the United Kingdom. Vet Rec 143: 225-227, 1998.
12. Cooke Graeme, Parker J. Screening for the CEM Organism. Vet Rec 152:442, 2003
13. Couto MA, Hughes JP. Sexually transmitted (venereal) disease of horses. In: Angus OM, James LV. ed. Equine reproduction. Lea & Febiger, Philadelphia, London, 845-854, 1993.
14. Crowhurst RC. Genital infection in mares. Vet Rec 100: 476, 1977.
15. Croxton-Smith P, Benson JA, Dawson FLM,Powell DG. A complement fixation test for antibody to the contagious equine metritis organism. Vet Rec 103: 275-278, 1978.
16. Doig PA, Waelchli RO. Endometrial biopsy. In: Angus OM, James LV. ed. Equine reproduction. Lea & Febiger, Philadelphia, London, 225-233, 1993.
17. Eguchi M, Kuniyasu C, Kishima M. Passive hemagglutination test for detection of antibodies against Taylorella equigenitalis in sera of mare. Vet. Microbiol. 18: 155-161, 1988.
18. Evans MJ, Hamer JM, Gason LM, Irvine CHG. Factors affecting uterine clearance of inoculated materials in mares. J. Reprod. Fert., Suppl. 35: 327-334, 1987.
19. Goodfellow M, O'Dpnnell AG. Roots of bacterial systematics. In: Goodfellow M, O'Dpnnell AG.. Handbook of new bacterial systematics, 2th ed. Academic Press, London. 3-56, 1994.
20. Greenhof GR, Kenney RM. Evaluation of reproductive status of nonpregnant mares. J. Am. Vet. Med. Assoc 167: 449-458, 1975.
21. Hinrichs K, Cummings MR, Sertich PL, Kenney RM. Clinical significance of aerobic bacterial flora of the uterus, vagina, vestibule, and clitoral fossa of clinically normal mares. J Am Vet Med Assoc 193: 72-75, 1988.
22. Katz JB, Evans LE, Hutto DL, Linda CST, Carew AM, Donahue JM, Hirsh DC. Clinical, bacteriologic, serologic, and pathologic features of infections with atypical Taylorella equigenitalis in mares. J Am Vet Med A 216: 1945-1948, 2000.
23. Kikuchi T, Iguchi I, Hiramune T. Capsule types of Klebsiella pneumoniae isolated from the genital tract of mares with Metritis, extra-genital sites of healthy mares and the genital tract of stallions. Veterinary microbiology 15: 219-228, 1987.
24. Mackintosh ME. Bacteriological techniques in the diagnosis of equine genital infections. Vet Rec 108: 52-55, 1981.
25. Matsuda M, Moore JE. Recent advances in molecular epidemiology and detection of Taylorella equigenitalis associated with contagious equine metritis (CEM). Vet Microbiology 97: 111-122, 2003.
26. Mitchell G, Liu IKM, Perrtman LE, Stabenfeldt GH Hughes JP. Preferential production and secretion of immunoglobulins by the equine endometrium - a mucosal immune system. J. Reprod. Fert., Suppl 32: 161-168, 1982.
27. Nikolakopoulos E, Watson ED. Uterine contractility is necessary for the clearance of intrauterine fluid but not bacteria after bacterial infusion in the mare. Theriogenology 52: 413-423, 1999.
28. Odumeru JA, Steele M, Fruhner L, Larkin C, Jiang J, Mann E, McNAB WB. Evaluation of accuracy and repeatability of identification of food-borne pathogens by automated bacterial identification systems J Clin Microbiol 37: 944-949, 1999.
29. Olson JD, Ball L, Mortimer RG, Farin PW, Adney WS, Huffman EM. Aspects of bacteriology and endocrinology of cows with pyometra and retained fetal membranes. Am J Vet Res 45: 2251-2255, 1984.
30. Ozgur NY, Ikiz S, Carioglu B, Kilicarslan R, Yilmaz H, Akay O, Ilgaz A. Contagious equine metritis in Turkey: first isolation of Taylorella equigenitalis from mares. Vet Rec 149: 120-122, 2001
31. Palys T, Nakamura LK, Cohan FM. Discovery and classification of ecological diversity in the bacterial word: the role of DNA sequence data. Int J Syst Bacteriol 47: 1145-1156, 1997.
32. Premanandh J, George LV, Wernery U, Sasse J. Evaluation of a newly developed real-time PCR for the detection of Taylorella equigenitalis and discrimination from T. asinigenitalis. Veterinary microbiology 95: 229-237, 2003.
33. Quinn PJ, Markey BK, Carter ME, Donnelly WJ, Leonard FC. Chapter 18 and 19. In: Veterinary microbiology and Microbiol disease. Blackwell Science Ltd, London, 121-126, 2002.
34. Rickeets SW, Mackintosh ME. Role of anaerobic bacteria in equine endometritis. J. Reprod. Fert., Suppl 35: 343-351, 1987.
35. Ricketts SW, Young A, Medici EB. Uterine and clitoral cultures. In: Angus OM, James LV. ed. Equine reproduction. Lea & Febiger, Philadelphia, London, 234-244, 1993.
36. Ricketts SW. Bacteriological examinations of the mare's cervix: Techniques and interpretation of results. Vet Rec 108: 46-51, 1981.
37. Ricketts SW. Klebsiella aerogenes in mares. Vet Rec:99 489-490, 1976.
38. Rigby SL, Barhoumi R, Burghardt PC, Colleran P, Thompson JA, Varner DD, Blanchard TL, Brinsko SP, Tarlor T, Wilkerson MK, Delp MD. Mares with delayed uterine clearance have an intrinsic defect in myometrial function. Biology of reproduction 65:740-747, 2001.
39. Sahu SP, Pierson RE, Dardiri AH. Contagious Equine Metritis: effect of intrauterine inoculation of contagious equine metritis agent in pony mares. Am J Vet Res 41: 5-9, 1980.
40. Sahu SP, Wool S, Breese SS. Observation on the morphology of contagious equine metritis bacterial colonies isolated from infected pony mares. Am J Vet Res 43:796-780, 1982.
41. Shin SJ, Lein DH, Aronson AL, Nusbaum SR. The bacteriological culture of equine uterine contents, in-vitro sensitivity of organisms isolated and interpretation. J. Reprod. Fert., Suppl. 27: 307-315, 1979.
42. Simons MAP. Contagious equine metritis. Vet Rec 102: 43, 1978.
43. Stackebrandt E, Goebel BM. A Place for DNA-DNA reassociation and 16SrRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846-849, 1994.
44. Stager CE, Davis JR. Automated systems for identification of microorganisms. Clin Microbiol Rev 5: 302-327, 1992.
45. Stager CE, Davis JR. Automated systems for identification of microorganisms. Clin Microbiol Rev 5: 302-327, 1992.
46. Strzemienski PJ, Benson CE, Acland HM, Kenney RM. Comparison of uterine protein content and distribution of bacteria in the reproductive tract of mares after intrauterine inoculation of Haemophilus equigenitalis or Pseudomonas aeruginosa. Am J Vet Res 45: 1109-1114, 1984.
47. Strzemienski PJ, Do D, Kenney RM. Antibacterial activity of mare uterine fluid. Biolog of reproduction 31:301-311, 1984.
48. Suzuki K, Goodfellow M, O'Donnell AG. Cell envelopes and classification. In: Goodfellow M, O'Donnell AG. Handbook of new bacterial systemics, 2th ed. Academic Press, London. 195-250, 1994.
49. Swaney LM, Sahu SP. CEM: Bacteriological methods. Vet Rec 102:43, 1978.
50. Swerczek TW. Elimination of CEM organisms from mares by excision of clitoral sinuses. Vet Rec 105: 131-132, 1979.
51. Terlaak EA, Wangenaars CMF. Autoagglutination and the specificity of the indirect fluorescent antibody test applied to the identification of Taylorella equigenitalis. Res Vet Sci 49: 117-119, 1990.
52. Thoresen SI, Jenkins A, Ask E. Genetic homogeneity of Taylorella equigenitalis from Norwegian trotting horses revealed by chromosomal DNA fingerprinting. J Clin Microbiol 33:233-234, 1995.
53. Timoney PJ, Ward J. A contagious genital infection of mares. Vet Rec 101: 103, 1977.
54. Timoney PJ. Contagious equine metritis. Comp. Immun. Microbiol. Infect. Dis. 19: 199-204, 1996.
55. Troedsson MHT, Liu IKM, ING M, Pascoe J, Thurmond M. Multiple site electromyography recordings of uterine activity following an intrauterine bacterial challenge in mares susceptible and resistant to chronic uterine infection. J. Reprod. Fert. 99: 307-313, 1992.
56. Troedsson MHT, Scott MA, Liu IKM. Comparative treatment of mares susceptible to chronic uterine infection. Am J Vet Res 56: 468-472, 1995.
57. Troedsson MHT. Uterine clearance and resistance to persistent endometritis in the mare. Theriogenology 52: 461-471, 1999.
58. Vandamme P, Pot B, Gillis M, Kerster K,Swings J. Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60: 407-438, 1996.
59. Watson ED. Swabbing protocols in screening for contagious equine metritis. Vet Rec 140: 268-271, 1997.
60. Widders PR, Warner S, Huntington PJ. Immunisation of mares to control endometritis caused by Streptococcus zooepidemicus. Res. Vet Sci 58: 75-81, 1995.
61. Wilks M, Stokes CR, David JSE, Bourne FJ, Ricketts SW. Quantitative bacteriology of the vaginal flora in genital disease. J med Microbiol 18: 217-231, 1984.
62. Wingfield Digby NJ, Ricketts SW. Results of concurrent bacteriologic and cytologic examinations of the endometritium of mares in routine stud farm practice1978-1981. J. Reprod. Fertil. Suppl. 32: 181-185, 1982.
63. Witherspoon DM, Goldston RT, Adsit ME. Uterine culture and biopsy in the mare. J Am Vet Med Assoc 161: 1365-1366, 1972.
64. Woese CR. Bacterial evolution. Microbiol Rev 51: 221-271, 1987.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top