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研究生:李寶琴
研究生(外文):Bao-Chin Li
論文名稱:以反射吸收式紅外光譜分析技術探討氣/液界面上DPPC/Albumin混合分子層行為的研究
論文名稱(外文):A Study of the Mixed DPPC/Albumin Layer Behavior at the Air/Liquid Interfaces by Infrared Reflection-Absorption Spectroscopy
指導教授:張鑑祥張鑑祥引用關係
指導教授(外文):chien - hsiang chang
學位類別:碩士
校院名稱:國立成功大學
系所名稱:化學工程學系碩博士班
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:112
中文關鍵詞:氣液界面單分子層反射吸收式紅外光譜分析技術
外文關鍵詞:DPPCalbuminIRRASIR
相關次數:
  • 被引用被引用:7
  • 點閱點閱:166
  • 評分評分:
  • 下載下載:8
  • 收藏至我的研究室書目清單書目收藏:0
中文摘要

  本研究利用反射吸收式紅外光譜分析技術,探討於動態壓縮-擴張氣液界面面積的情況下,albumin對DPPC分子層行為的影響。
在空氣/albumin水溶液界面上,DPPC單分子層的碳氫鏈排列會因為albumin水溶液濃度的不同而受到不同程度的影響。當albumin水溶液濃度為10 ppm時,在壓縮-擴張氣液界面的過程中,DPPC碳氫鏈的排列行為與氣液界面上單純只有DPPC存在時的行為相似,顯示低濃度的albumin水溶液對氣液界面上DPPC碳氫鏈的排列位向影響較小,且在經過第一次壓縮後,於氣液界面擴張過程中albumin分子再吸附於界面上的量較少。反之,高濃度的albumin水溶液會使界面上DPPC分子排列更緊密,且分子碳氫鏈的排列位向更垂直於氣液界面,而存於氣液界面上自由DPPC分子的量,隨著albumin濃度增加而減少。此外,在高濃度的albumin水溶液中,albumin吸附於氣液界面上的速率較快,使得在三次壓縮擴張氣液界面的過程中,界面上自由的DPPC分子持續處於排列較緊密的狀態。
  經由改變DPPC及albumin分子層的分佈次序,並從事後續分子層的壓縮-擴張實驗,也發現界面上DPPC分子碳氫鏈的排列位向及緊密程度會受到分佈次序的影響。
Abstract

  This study investigated the influence of albumin on dipalmitoyl phosphatidylcholine (DPPC) monolayer behavior at cyclic air/liquid interfaces by infrared reflection-absorption spectroscopy (IRRAS).
  For a DPPC monolayer at the interfaces of air/albumin solutions, the orientation of DPPC hydrocarbon chains was affected with the extent dependent on albumin concentration. When the albumin concentration was 10 ppm, during the interface compression/expansion, the orientation of DPPC hydrocarbon chains was similar to that obtained for a DPPC monolayer on a pure water subphase. This result indicated that with lower albumin concentrations, the orientation of DPPC hydrocarbon chains was affected insignificantly and the amount of albumin re-adsorption during interface expansion could be neglected. On the contrary, with higher albumin concentrations, DPPC molecules at the interface were close-packed and the orientation of DPPC hydrocarbon chains became vertical to the interface. The amount of free DPPC molecules at interfaces was found to decrease with increasing albumin concentration. Moreover, for an albumin subphase with higher concentrations, the adsorption rate of albumin at the interface was faster. As a result, the free DPPC molecules at the interface were always close-packed during three cycles of interface compression/expansion.
  By changing the spreading order of DPPC and albumin at the interface to form mixed monolayers, it was found that the hydrocarbon chain orientation and surface density of DPPC molecules were affected by the spreading order, as judging from the experimental results.
中文摘要 I
英文摘要 II
致謝 III
總目錄 IV
表目錄 VII
圖目錄 IX
符號說明 XVIII

第一章 緒論 1
1.1前言 1
1.2文獻回顧 4
1.2.1反射吸收式紅外光譜分析技術應用於Langmuir 單分子層的研究 4
1.2.2反射吸收式紅外光譜分析技術應用於肺泡界面活性劑的研究 5
1.2.3反射吸收式紅外光譜分析技術應用於血漿蛋白質的研究 7
1.3研究動機與目的 8

第二章 實驗 9
2.1藥品 9
2.2儀器 9
2.2.1衰減全反射式紅外光譜儀分析技術 9
2.2.2反射吸收式紅外光譜分析技術 11
2.3實驗方法 14
2.3.1衰減全反射式紅外光譜的測量 14
2.3.2反射吸收式紅外光譜的測量 15
2.3.2.1 DPPC單分子層遲滯行為的測量 15
2.3.2.2 DPPC/albumin混合分子層動態吸附平衡的測量 16
2.3.2.3 DPPC/albumin混合分子層遲滯行為的測量 17
2.3.2.4 DPPC/albumin吸附分子層動態吸附平衡的測量 17
2.3.2.5 DPPC/albumin吸附分子層遲滯行為的測量 17
2.3.3反射吸收式紅外光譜儀的操作 17

第三章 技術建立 25
3.1儀器條件測試 25
3.2實驗的改良設計 27
3.3目前對於albumin IR光譜的偵測結果 31
3.4後續實驗的建議 32

第四章 結果與討論 46
4.1 DPPC與albumin的FTIR-ATR紅外光譜 46
4.2氣液界面上DPPC單分子層的IRRAS紅外光譜 47
4.3氣液界面上albumin分子層的IRRAS紅外光譜 55
4.4氣液界面上DPPC/albumin混合系統的IRRAS紅外光譜 56
4.4.1 DPPC在albumin吸附分子層中的IRRAS紅外光譜 56
4.4.2 混合分佈DPPC/albumin分子層的IRRAS紅外光譜 59
4.4.2.1先分佈DPPC分子層 59
4.4.2.2先分佈albumin分子層 60
第五章 結論與建議 96
5.1結論 96
5.2建議 97

參考文獻 99
附錄 107
自述 112
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