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研究生:江武達
研究生(外文):Wu-Da Jiang
論文名稱:馬拉松賽對粒線體膜電位及細胞自發性死亡影響之研究
論文名稱(外文):The Effect of Marathon Race on both Disruption of Mitochondria Transmembrane Potential and Apoptosis in Human Peripheral Blood
指導教授:徐台閣
指導教授(外文):Tai-Ger Hsu
學位類別:碩士
校院名稱:國立體育學院
系所名稱:運動科學研究所
學門:民生學門
學類:運動科技學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:55
中文關鍵詞:粒線體膜電位細胞自發性死亡馬拉松賽
外文關鍵詞:mitochondria transmembrane potentialapoptosismarathon race
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馬拉松賽對粒線體膜電位及
細胞自發性死亡影響之研究

摘要

適當的運動能增進體適能及健康外,也可增進生活品質,但是在高強度耐力運動時,因耗氧量的增加,導致體內產生過多的活性氧物種 (reactive oxygen species,ROS),當身體機能無法將過多活性氧轉換成氧分子和水時;則造成氧化傷害,其毒性產物的逐漸累積會造成細胞及組織不可逆的氧化傷害。本研究目的在探討馬拉松比賽,對人體粒線體膜電位及細胞自發性死亡的影響。以參加2003年台北國道國際馬拉松賽15位男選手為受試對象,檢測受試者比賽前、比賽後30分鐘、24小時、72小時及一週,血液中淋巴球細胞自發性死亡的比例、粒線體膜電位以及脂質過氧化的影響。本研究採用同一受試者重複量數之設計,受試者血液分析以單因子變異數分析(one-way ANOVA),若達顯著差異時,則利用薛費法進行事後比較。本研究發現受試者在跑完馬拉松賽後粒線體的膜電位 (Δym),明顯下降 (p < .05),顯見高強度耐力運動會造粒線體膜電位下降。多形核白血球 (polymophonu clear leukocyte,PMN) 細胞自發性死亡的比率也較賽前明顯增高(p < .05),而在跑後第三天跑後達到最高值。脂質過氧化 (Lipid peroxidation,LPO) 的濃度明顯增加,表示這種強度的運動,對耐力運動員而言會造成氧化傷害。本研究的結論是馬拉松賽會造成氧化傷害,因此賽後需注意身體保健,以免造成身體不適。

關鍵詞:馬拉松賽、細胞自發性死亡、粒線體膜電位。
The effect of marathon race on both disruption of mitochondria transmembrane potential and apoptosis in human peripheral blood

Abstract

Appropriate exercise can promote not only health but also the quality of life. But heavy physical exercise may produce excessive reactive oxygen species and cause damages. In previous study, Tai-Ger Hsu et al. indicated that the heavy physical exercise actually cause the accumulated damage.
The purpose of this study was to evaluate time-sequence changes in the mitochondria transmembrane . We collect the data pre-race,30 min,24 hour,72 hour and a week after the marathon race. One-way repeated ANOVA was used to testing the data we collection. The alpha level was set at .05.
We find that the mitochondria transmembrane potential (MTP) declined immediately post-race (p < 0.5), polymophonu clear leukocyte (PMN) gradually increased during the postexercise and reached supranormal levels in 72 hours affter the race, and the density of lipid peroxidation (LPO) increased obviously.We may conclude that the body functions will be disturbanced and it will decrease the cell activities.

Key words: marathon race, apoptosis,
mitochondria transmembrane potential
目 錄

第壹章 緒論………………………………………… 1

第一節 前言 ………………………………………… 1
第二節 研究背景…………………………………… 2
第三節 研究的重要性 ……………………………… 4
第四節 研究目的…………………………………… 4
第五節 研究假設……………………………………… 4
第六節 研究範圍 …………………………………… 4
第七節 研究限制……………………………………… 5
第八節 名詞操作性定義……………………………… 5

第貳章 文獻探討…………………………………… 7

第一節 粒線體膜電位………………………………… 8
第二節 脂質過氧化…………………………………… 12
第三節 細胞自發性死亡……………………………… 14
第四節 結語…………………………………………… 16

第叁章 研究方法與步驟…………………………… 18

第一節 研究對象 …………………………………… 18
第二節 研究設計與實驗變項 ……………………… 18
第三節 實驗方法……………………………………… 19
第四節 實驗的儀器…………………………………… 20
第五節 細胞粒線體膜電位、細胞自發性死亡…… 21
第六節 脂質過氧化分析……………………………… 24
第七節 實驗控制……………………………………… 24
第八節 統計分析……………………………………… 25

第肆章 結果……………………………………………26

第一節 受試者………………………………………… 26
第二節 粒線體膜電位的變化………………………… 26
第三節 細胞脂質過氧化的變化……………………… 31
第四節 細胞自發性死亡的變化……………………… 33

第伍章 討論 ………………………………………… 36

第一節 粒線體膜電位與細胞自發性死亡的影響…… 36
第二節 脂質過氧化與細胞自發性死亡的影響……… 39

第陸章 結論……………………………………………41

參考文獻…………………………………………………42

附錄
一 受試者須知及同意書…………………………………51
二 受試者健康情況調查表………………………………52
三 馬拉松比賽前、後粒線體膜電流式細胞儀分析圖 53


表 目 錄

表4-1受試者基本資料表………………………………………… 27
表4-2馬拉松比賽前、後多型核細胞粒線體膜電位的變化表… 28
表4-3馬拉松比賽前、後單核球細胞粒線體膜電位的變化表… 30
表4-4馬拉松比賽前、後淋巴球細胞粒線體膜電位的變化表… 31
表4-5馬拉松比賽前、後血漿中丙二醛的變化表……………… 33
表4-6馬拉松比賽前、後多型核細胞自發性死亡變化表……… 34
表4-7馬拉松比賽前、後單核球細胞自發性死亡變化表……… 36




圖 目 錄

圖3-1抽血步驟與流程圖………………………………………… 20
圖3-2流式細胞分析儀 (flow cytometry) …………………… 21
圖3-3酵素免疫分析儀 (ELISA reader) ……………………… 21
圖4-1馬拉松比賽前、後多型核細胞粒線體膜電位的變化圖… 29
圖4-2馬拉松比賽前、後單核球細胞粒線體膜電位的變化圖… 30
圖4-3馬拉松比賽前、後淋巴球細胞粒線體膜電位的變化圖… 31
圖4-4馬拉松比賽前、後血漿中丙二醛的變化圖……………… 33
圖4-5馬拉松比賽前、後多型核細胞自發性死亡變化圖……… 35
圖4-6馬拉松比賽前、後單核球細胞自發性死亡變化圖……… 36
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