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研究生:林禎祥
研究生(外文):Tzun-Sheang Lin
論文名稱:夜來香組培苗發根、田間植株性狀表現及核型分析之研究
論文名稱(外文):Rooting of In Vitro Plantlets, Field Characteristic Performance of In Vitro Rooted Plants and Karyology of Polianthes tuberosa L.
指導教授:沈再木沈再木引用關係
指導教授(外文):Tsai-Mu Shen
學位類別:碩士
校院名稱:國立嘉義大學
系所名稱:園藝學系碩士班
學門:農業科學學門
學類:園藝學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:129
中文關鍵詞:夜來香發根切花產量染色體數核型分析
外文關鍵詞:Polianthes tuberosa L.rootingcut flower yieldchromosome countkaryology analysis
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夜來香組培苗發根、田間性狀表現及核型分析之研究
摘要
以Modified MS基礎培養基中分別添加IBA或NAA誘導夜來香‘重瓣’及‘單瓣’品種瓶內不定根之發生,結果顯示低濃度處理即可有效的誘導不定根的發生,產生有正常功能的根毛,並促進瓶苗生長及提高出瓶存活率。但NAA 1.0 mg/l之高濃度處理,根部生長量被促進,根部TTC還原反應增加,根數減少、根伸長受抑制,芽體葉綠素含量及葉片TTC還原力則較低,芽體的生長明顯受到抑制,推測此為造成瓶苗出瓶存活率低僅達28.2~34.2%之原因。夜來香‘單瓣’品種未發根之試管苗移出於瓶外進行發根試驗,結果顯示,生長素的添加並非為決定成活與否的關鍵因素,苗株的大小影響較大,當芽體直徑達2mm以上時,不論是否有處理生長素其存活率均可達55~64%。而瓶內發根之苗株,出瓶存活率顯著較直接瓶外發根者為佳。
夜來香‘單瓣’品種依其母球來源及不同塊莖的大小以比較其對切花產量及品質之影響,結果顯示,組織培養球不論母球大小為何,單株切花產量均較田間久經無性繁殖者為高,但切花品質而言,各處理間差異不大。夜來香‘重瓣’品種組織培養苗經出瓶成活後,直接種植於田間並與無性繁殖母球直徑0.5及2.0 cm之植株,進行切花品質比較,顯示花序長及花蕾數上差異不大;而組織培養苗有13.3%的植株產生較長花莖的型式,花莖長度顯著長於其他處理者。
夜來香核型分析結果顯示夜來香‘單瓣’ 2n=60=10L+50S,‘重瓣’2n=54=10L+2M1+42S;雜交選育之品種夜來香‘嘉農紅海’ 2n=56=2L+6M2+12M1+36S及夜來香‘嘉農小精靈’ 2n=56=6L+2M2+4M1+44S;P. howardii 2n=58=10L+6M1+42S;其染色體數目有變化,且與親本相較,有大染色體減少,而中、小染色體增加的現象。

Rooting of in vitro plantlets, field characteristic performance of in vitro rooted plants and karyology of Polianthes tuberosa L.
Abstract
The modified MS medium supplemented with IBA or NAA was used to induce adventitious root formation of Polianthes tuberosa ‘Double’and ‘Single’. Low concentration of IBA or NAA could induce root formation effectively, and produced normal functional root hair to improve the growth of plantlets in vitro and the survival rate rooted plantlets of in vivo conditions. However, high NAA concentration at 1.0 mg/l increased biomass of root and TTC reduction ability, decreased number of roots, and inhibited of root elongation. The chlorophyll content of shoot and TTC reduction ability of leaf was low. Therefore, shoot growth was significantly inhibited. It was supported that low survival rate (28.2-34.2%) of rooted plantlets was influenced by high concentration of NAA. In vitro plantlets without root were transplanted for rooting in vivo conditions and results showed that the addition of auxins was not required for plantlet survival. The size of shoot was a more critical factor. Regardless of the addition of auxins, the survival rate could reach 55-64% when shoots at 2mm or more in diameter. The survival rate on in vitro rooted plantlets was higher than those of in vitro plantlets without root.
The effects of different sources and tuber sizes of tuberose stock were studied on the yield and quality of cut flower. Tubers of ‘Single’produced from tissue culture, regardless of tuber size, had higher yield of cut flower than those of tubers produced from traditional vegetative division. However, there was no significant difference in quality of cut flower. In ‘Double’cultivar, there was no significant difference in length of flower spike, number of flower bud and performance of cut flower between plants produced from tissue culture and plants produced from traditional vegetative division by tubers 0.5 and 2.0cm in diameter. However, 13.3% plants produced from tissue culture showed significantly longer flower stalks.
Karyology analysis of P. tuberosa‘Single’showed that the chromosome count 2n=60, with 10L+50S, and P. tuberosa‘Double’ 2n=54, 10L+2M1+42S. However, the hybrids of P. tuberosa‘Chia-Nong Pixie’had chromosome count at 2n=56, 6L+2M2+4M1+44S, P. tuberosa‘Chia-Nong Red Sea’ 2n=56, with 2L+6M2+12M1+36S, P. howardii 2n=58, 10L+6M1+42S. Comparing with the originated species, the hybrids had fewer in large chromosomes, and increase in medium and small chromosomes.
Key words:Polianthes tuberosa L., rooting, cut flower yield, chromosome count, karyology analysis.

目錄(Content)
P.
壹、 前言(Introduction)……………………………………………1
貳、 前人研究(Literature review)
一、 瓶苗發根…………………………………………………………3
(一).生長素濃度梯度之影響………………………………………………3
(二).生長素對於枝梢及根部發育之影響…………………………………4
(三).瓶內發根之效益………………………………………………………6
(四).生長素濃度及種類對瓶苗生育之影響………………………………7
(五).生長素施用方式…………………………………………………….11
(六).木本及草本植物不定根發生之差異性…………………………….12
(七).組織培養時根部發生之過程……………………………………….14
(八).不定根發生之基因分析…………………………………………….15
(九).組織培養時根毛之發育…………………………………………….16
(十).組織TTC還原性測定………………………………………………..18
(十一).糖類吸收對葉率素含量之影響………………………………….24
二、 夜來香之分類地位及遺傳背景……………………………….25
(一).夜來香之分類地位………………………………………………….25
(二).夜來香之育種……………………………………………………….26
三、 夜來香病毒感染情形………………………………………….27
四、 染色體核型分析……………………………………………….29
(一).染色體之形成……………………………………………………….30
(二).核型分析前處理原理……………………………………………….32
參、 材料與方法(Materials and methods)
一、 夜來香瓶苗發根試驗……………….………………………..34
二、 夜來香瓶苗植株性狀觀察……….……………………………39
三、 夜來香核型分析…………………….…………………………42
肆、 結果(Results)
一、 夜來香瓶苗發根試驗…………………….……………………44
(一).生長素濃度試驗…………………………………………………….44
(二).葉綠素含量分析…………………………………………………….45
(三).組織TTC還原力測定…………………………………………………45
(四).根形態及根毛功能觀察…………………………………………….46
(五).生長素處理對瓶苗出瓶種植之影響……………………………….47
(六).瓶外發根試驗……………………………………………………….47
(七).瓶內及瓶外發根苗之比較………………………………………….48
二、 組培苗植株性狀比較………………………………………….48
(一).夜來香 ‘單瓣’(P. tuberosa‘Single’)…………………….48
(二).夜來香 ‘重瓣’(P. tuberosa‘Double’)…………………….51
三、 夜來香核型分析……………………………………………….52
(一).染色體大小………………………………………………………….52
(二).核型………………………………………………………………….52
伍、 討論(Discussion)…………………………………………….93
陸、 參考文獻(References)……………………………………..110
附錄(Appendix)…………………………………………………….… .126

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