跳到主要內容

臺灣博碩士論文加值系統

(3.235.174.99) 您好!臺灣時間:2021/07/24 18:47
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

: 
twitterline
研究生:邱鈺珊
研究生(外文):Yu-Shan Chiou
論文名稱:表面DNA分子之氣相與液相原子力顯微術成像研究
論文名稱(外文):Molecular-Scale Imaging of Surface-Bound DNAs Using Atomic Force Microscopy in Air and Liquids
指導教授:蔣昭明
指導教授(外文):chao-ming chiang
學位類別:碩士
校院名稱:國立中山大學
系所名稱:化學系研究所
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:76
中文關鍵詞:去氧核醣核酸雲母原子力顯微鏡
外文關鍵詞:micaDNAatomic force microscopy
相關次數:
  • 被引用被引用:1
  • 點閱點閱:153
  • 評分評分:
  • 下載下載:11
  • 收藏至我的研究室書目清單書目收藏:0
以原子力顯微鏡(AFM)於氣相及液相環境觀察環圈形並已知鹼基對數目的質體DNA(pQE70),在緩衝溶液中控制離子鹽濃度輔以加熱或適當鬆弛試劑可消除DNA的聚集和摺疊,而在平坦的雲母基板掃描到環圈狀的DNA影像,由高度及寬度的分析,確認為單一的DNA分子。氣相AFM所測量到的pQE70長度(contour length)與B型DNA一致,但在異丙醇掃描介質中測量長度增長為B型的2.4倍之多。探討化學修飾對於雲母表面固定DNA能力的改善情形,比較利用醋酸鎂、氯化鋇和3-aminopropyl-triethoxysilane(APTES)改變表面電性後對DNA吸附及成像之效果。針對單股及雙股噬菌體DNA(M13mp18)作影像比對,發現兩者在AFM解析度限制下具有可茲辨識的特徵。
摘要 i
目錄 ii
圖表目錄 v

第壹章、緒論 1
1-1研究背景 1
1-2核酸 1
1-3 DNA的拓樸結構 6
1-4原子力顯微鏡 7
原子力顯微鏡之探針 8
原子力顯微鏡在生物學上的應用 10
原子力顯微鏡在生物學應用上的優缺點 11
研究動機 12
第貳章、實驗 14
2-1實驗儀器 14
2-2原子力顯微鏡原理 14
2-2-1 Contact mode(接觸模式) 16
2-2-2 Tapping mode(輕敲模式) 17
2-2-3液相掃描 18
液相AFM的操作步驟 18
調整探針共振頻率與振幅 20
2-2-4原子力顯微鏡偵測模式與探針選擇 22
2-3材料 24
2-3-1<a>基板 24
<b>樣品 24
<c>樣品配製的生物緩衝液及濃度 24
<d>AFM的Liquid Cell中液相環境中所使用的溶液 24
2-4實驗操作方法 29
2-4-1方法簡介 29
2-4-2實驗流程 30
2-4-3樣品配製 31
滅菌水 31
醋酸鎂將Mica表面修飾成帶正電的Mica-Mg(II) 31
以BaCl2將Mica表面修飾成帶正電的Mica-Ba(II) 32
AP-mica的製備 32
2-4-4 Q & A 34
第參章、結果與討論 36
3-1雲母基板(Mica substrate) 36
3-2氣相AFM觀察雲母基板上的DNA分子 37
3-3環圈狀DNA的長度測量 43
3-4液相AFM觀察雲母基板上的DNA分子 45
3-5利用化學試劑使纏繞的DNA鬆弛 49
3-5-1 以formamide 作為DNA鬆弛試劑 49
3-5-2 以ammonium acetate作為鬆弛試劑 52
3-6液相AFM所觀察到環圈狀DNA長度 53
3-7將雲母修飾成帶正電的表面 59
3-8 DNA超螺旋 64
3-9單股及雙股噬菌體DNA 65
3-9-1單股M13mp18 DNA 66
3-9-2雙股M13mp18 DNA 67
第肆章、結論 71
參考文獻 74
1. Morris; Kirby; Gunning Atomic Force Microscopy for Biologists; Imperial College Press, 1999
2.Digital Instrument Inc. MultimodeTM PicoForceTM Manual.
3.曾文聖、林良平 “科儀新知” 1998, 6, 4
4.Dickerson, R. E. Quant. Biol. 1982, 47 ,14
5.McKee, J. R.; McKee, T. Biochemistry (The Molecular Basis of Life Third Edition)
6.Conn; stumpf; Bruening; Doi Outline of Biochmistry 藝軒
7.譚景瑩、董志偉 “英漢生物化學及分子生物學詞典” 藝軒
8.Rugar, D.; Hansma, P. K. Phys. Today 1990, 23:Atomic Force Microscopy
9.Minne, S. C.; Manalis, S. R.; Quate, C. F. Bringing Scanning Probe Microscopy up to Speed, Kluwer Academic Publishers, 1999
10.Hansma, H. G. Annual review of physical chemistry 2001, 52, 71:Surface Biology of DNA by Atomic Force Microscopy
11.Bezanilla, M.; Hansma, P. K. Biophys. J. 1994, 67, 2454:Motion and Enzymatic Degradation of DNA in the Atomic Force Microscope
12.白春禮 “來自微觀世界的新概念-單分子科學與技術” 牛頓
13.Digital Instrument Inc. Chinese Manual
14.林鶴南、李龍正、劉克迅 “科儀新知” 1995, 12, 29
15.Digital Instrument Inc. Supporting Note
16.Hansma, H. G.; Sinsheimer, R. L.; Li, M. Q.; Hansma, P. K. Nucleic Acids Res. 1992 20, 3585:Atomic Force Microscopy of Single- and Double-Stranded DNA
17.Thundat, T.; Allison, D. P.; Warmack, R. J.; Doktycz, M. J.; Jacobson, K. B.; Brown, G. M. J. Vac. Sci. Technol. A 1993, 824:Atomic Force Microscopy of Single- and Double-Stranded Deoxyribonucleic Acid
18.Lyubcheno, Y. L.; Gall, A. A.; Shlyakhtenko, L. S.; Harrington, R. E.; Jacobs, B. L.; Oden, P. I.; Lindsay, S. M. J. Biomol. Struct. Dyn. 1992, 10, 589:Atomic Force Microscopy Imaging of Double Stranded DNA and RNA
19.SIGMA ProductInformation
20.Daugharty, H.; Long, E. G.; Swisher, B. L.; Warfield, D. T.; Feorino, P. M. J. Clin. Lab. Analysis 1990, 4, 283:Comparative Study with in Situ Hybridization and Immunocytochemistry in Detection of HIV-1 in Formalin-Fixed Paraffin-Embedded Cell Cultures
21.Digital Instrument Inc. Instruction Manual
22.Tsujimichi, K.; Tamura, H.; Hirotani, A.; Kubo, M.; Komiyama, M.; Miyamoto, A. J. Phys. Chem. B 1997, 101, 4260:Simulation of Atomic Force Microscopy Image of Cleaved Mica Surfaces
23.Tang, H.; Joachim, C.; Devillers, J. J. Vac. Sci. Technol. B 1994, 12, 2179:Interpretation of Atomic Force Microscopy Images:The Mica (001) Surface with a Diamond Tip Apex
24.Darake, B.; Prater, C. B.; Weisenhorn, A. L.; Gould, S. A. C.; Albrecht, T. R.; Quate, C. F.; Canell, D. S.; Hansma, H. G.; Hansma, P. K. Science 1989, 243, 1586:Imaging Crystals, Polymers, and Processes in Water with the Atomic Force Microscope
25.Murray, M. N.; Hansma, H. G.; Bezanilla, M.; Sano, T.; Ogletree, D. F.; Kolbe, W.; Smith, C. L.; Cantor, C. R.; Spengler, S.; Hansma, P. K.; Salmeron, M. Proc. Natl. Acad. Sci. USA 1993, 90, 3811:Atomic Force Microscopy of Biochemically Tagged DNA
26.Thundat, T.; Allison, D. P.; Warmack, R. J.; Ferrell, T. L. Ultramicroscopy 1992, 42-44, 1101-1106:Imaging isolated strands of DNA Molecules by Atomic Force Microscopy
27Bustamante, C.;Vesenka, J.; Tang, C. L.; Rees, W.; Guthold, M.; Keller, R. Biochemistry 1992, 31, 22:Circular DNA Molecules Imaged in Air by Scanning Force Microscopy
28.Samorf, B.; Siligardi, G.; Quagliariello, C.; Weisenhorn, A. L.; Vesenka, J.; Bustamante, C. J. Proc. Natl. Acad. Sci. USA, 90, 3598:Chirality of DNA Supercoiling Assigned by Scanning Force Microscopy
29.Hansma, H. G.; Revenko, I.; Kim, K.; Laney, D. E. Nucleic Acids Res 1996, 24, 713:Atomic Force Microscopy of Long and Short Double-Stranded, Single-Stranded and Triple-Stranded Nucleic Acids
30.Vesenka, J.; Guthold, M.; Tang, C. L.; Keller, D.; Delaine, E.; Bustamante, C. Ultramicroscopy 1992, 42-44, 1243:Substrate Preparation for Reliable Imaging of DNA Molecules with the Scanning Force Microscope
31.Hansma, H. G.; Bezanilla, M.; Zenhausern, F.; Adrian, M.; Sinsheimer, R. L Nucleic Acids Res. 1993, 21, No.3, 505-512:Atomic Force Microscopy of DNA in Aqueous Solutions
32.Hansma, H. G.; Vesenks, J.; Siegerist, C.; Kelderman, G.; Morrett, H.; Sinsheimer, R. L.; Elings, V.; Bustamante, C.; Hansma, P. K. Science 1992, 256, 1180:Reproducible Imaging and Dissection of Plasmid DNA Under Liquid with the Atomic Force Microscope
33.Thundat, T.; Warmack, R. J.; Allison, D. P.; Bottomley, L. A.; Lourenco, A. J.; Ferrell, T. L. J. Vac. Sci. Technol. A 1992, 10, 630:Atomic Force Microscopy of Deoxyribonucleic Acid Strands Adsorbed on Mica : The Effect of Humidity on Apparent width and Image Contrast
34.Yang, J.; Takeyasu, K.; Shao, Z. FEBS 1992, 301, 173:Atomic Force Microscopy of DNA Molecules
35.Thundat, T.; Allison, D. P.; Warmack, R. J.; Brown, G. M.; Jacobson, K. B.; Schrick, J. J.; Ferrell, T. L. Scanning Microsc. 1992, 6, 911:Atomic Force Microscopy of DNA on Mica and Chemically Modified Mica
36.Cluzel, P.; Lebrun, A.; Heller, C.; Lavery, R.; Viovy, J. L.; Chatenay, D.; Caron, F. Science 1996, 271, 792:DNA: An Extensible Molecule
37.Léger, J. F.; Romano, G.; Sarkar, A.; Robert, J.; Bourdieu, L.; Chatenay, D.; Marko, J. F. Phys. Rev. Lett. 1999, 83, 5, 1066
38.Thundat, T.; Allison, D. P.; Warmack, R. J. Nucleic Acids Res. 1994, 22, 4224:Stretched DNA Structures Observed with Atomic Force Microscopy
39.Cheatham, T. E.; Crowley, M. F.; Fox, T.; Kollman, P. A. Proc. Natl. Acad. Sci. USA 1997, 94, 9626:A Molecular Level Picture of the Stailization of A-DNA in Mixed Ethanol-Water Solutions
40.Zehfus, M. H.; Johnson, W. C. Biopolymers 1984, 23, 1269:Conformation of P-Form DNA
41.Allemand, J. F.; Bensimon, D.; Lavery, R.; Croquette, V. Proc. Natl. Acad. Sci. USA 1998, 95, 14152:Stretched and Overwound DNA Forms a Pauling-Like Structure with Exposed Bases
42.Boyer,R Concepts in Biochemistry.
43.Bezanilla, M.; Mann, S.; Laney, D. E.; Lyubchenko, Y. L.; Hansma, H. G. Langmuir 1995, 11, 655:Adsorption of DNA to Mica, Silylated Mica, and Minerals: Charaterization by Atomic Force Microscopy
44.Hansma, H. G.; Sinsheimer, R. L.; Groppe, J.; Bruice, T. C.; Elings, V.; Gurley, G.; Bezanilla, M.; Mastrangelo, I. A.; Hough, V. C.; Hansma, P. K. Scanning 1993, 15, 296:Recent Advances in Atomic Force Microscopy of DNA
45.Seong, G. H.; Niimi, T.; Yanagida, Y.; Kobatake, E.; Aizawa, M. Anal. Chem. 2000, 72, 1288:Single-Molecular AFM Probing of Specific DNA Sequencing Using RecA-Promoted Homologous Pairing and Strand Exchange
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top