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研究生:吳靖穎
研究生(外文):Jing-ying Wu
論文名稱:台灣西海岸蚵岩螺族群Ark(argininekinase)同功酵素之時空變化
論文名稱(外文):Temporal and Spatial Variations of Arginine Kinase Allozyme Among Populations of Thais clavigera From the West Coasts of Taiwan
指導教授:劉莉蓮
指導教授(外文):Li-Lian Liu
學位類別:碩士
校院名稱:國立中山大學
系所名稱:海洋生物研究所
學門:自然科學學門
學類:海洋科學學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:46
中文關鍵詞:蚵岩螺族群遺傳時空變化同功酵素
外文關鍵詞:temporal and spatial variationarginine kinaseThais clavigerapopulation genetics
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前蚵岩螺族群遺傳研究顯示arginine kinase (Ark)基因型頻率在香山、台西、布袋、七股等四地點有分化的現象。本研究探討地點間Ark基因頻率的差異是否源自各地點有不同之補充群,以蛋白質電泳技術來檢測蚵岩螺個體的Ark基因型,探討蚵岩螺Ark基因型頻率在空間上(香山、台西、布袋、七股)及時間上(體型:<15mm, 15-20mm, 20-30mm, 30-40mm, >40mm及年間:2001, 2003)的變化。結果顯示補充進入各地點的補充群(<15mm)Ark基因型頻率(ark63/63, ark63/100, ark100/100),除布袋外皆無顯著差異,而在體型15-20mm類群ark63/63頻率有由南自北漸增的現象,分別為七股(0.21)<布袋(0.33)<台西(0.60)<香山(0.66),推測可能與對高溫或乾燥之耐受力有關;而雌雄間Ark基因型頻率差異最顯著在台西30-40mm類群,但Ark基因型頻率地點間差異則與雌螺雄化程度無關。
The variation of Arginine kinase (Ark) genotype frequency among populations of Thais clavigera has been reported in previous study. However, the difference which is originated from self-seeding or differential selection after recruitment is not understood. To answer this question, the spatial (i.e. Shainsan, Taisi, Budai, and Chiku) and temporal (sell length: <15mm, 15-20mm, 20-30mm, 30-40mm, and >40mm; year: 2001 and 2003) variations of Ark allozyme were examined by starch gel electrophoresis. There was no significant difference in the ratio of Ark genotypes (ark63/63, ark63/100, ark100/100) in the size range of <15mm among Shainsan, Taisi and Chiku. The result indicated that the populations of T. clavigera is from the same recruitment. In the size range of 15-20mm, the frequency of ark63/63 increased from southern to northern populations, i.e. 0.21(Chiku) < 0.33(Budai) < 0.60(Taisi) < 0.66(Shainsan), respectively. It is proposed that the variation of Ark genotypes among populations is resulting from stress of temperature or dessication. A significant difference in the ratio of Ark genotypes was found in the population of Taisi with the size range of 30-40mm. Besides, the frequency of Ark genotypes among populations was not related to the degree of imposex.
目錄章次 頁次
中文摘要……………………………………………………………Ⅲ
英文摘要……………………………………………………………Ⅳ
目錄…………………………………………………………………Ⅴ
表目錄………………………………………………………………Ⅵ
圖目錄………………………………………………………………Ⅶ
一、緒論……………………………………………………………1
二、材料與方法……………………………………………………5
三、結果……………………………………………………………8
四、討論……………………………………………………………14
參考文獻……………………………………………………………21
個人履歷……………………………………………………………46
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