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研究生:張仲杰
研究生(外文):Chung-Chieh Chang
論文名稱:S-layer功能化表面之研究
論文名稱(外文):Study on S-layer Functionalized Surfaces
指導教授:譚世特
指導教授(外文):Shih-Teh Tan
學位類別:碩士
校院名稱:國立清華大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:28
中文關鍵詞:表層結晶蛋白表面功能化耐輻射奇異球菌
外文關鍵詞:S-layersurface functionalizationDeinococcus radiodurans
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耐輻射奇異球菌Deinococcus radiodurans細胞表面結晶蛋白(S-layer protein, SLP)已知為126 kDa具自我組合能力的蛋白質分子。本研究中,我們以另一耐輻射奇異球菌野生型菌株Deinococcus radiodurans IR (輻射抗性與生長速率均較R1為高)為材料,對D. radiodurans IR SLP作自組合性質分析及應用層面的開發。
我們在低濃度氫鍵破壞劑 (hydrogen-bonds-breaking agent)存在下,成功展現D. radiodurans IR SLP在native silicon wafer表面的自組合能力 (self-assembly capacity)。此自組合產品經光學顯微鏡檢(optic microscopy)及共軛焦顯微鏡鏡檢(confocal laser scanning microscopy),發現為大面積規模覆蓋wafer的膜狀結構(Large-scale self-assembly film, Large-scale SAF)與多層次堆疊結構(Multi-layer self-assembly film, Multi-layer SAF)。原子力顯微鏡鏡檢(atomic force microscopy)顯示,SAF的表面形貌為等大小顆粒狀結構。
應用層面上,我們能夠使用不同的系統在經由SAF功能化的wafer表面直接合成Ag nanowire(直徑~50-100 nm)以及不同形態的Ag nanostructures。對於Ag nanowire生成的條件我們也作了一些修正,透過製程改進可以將Ag nanowire平均長度提升到90 ± 6.7 μm (aspect ratio > 1000)。
The surface layer (S-layer) of Deinococcus radiodurans composed of single protein, that molecular weight is about 126 kDa. In this article, we got some investigations about properties of the D. radiodurans IR S-layer protein (SLP) self-assembled on native silicon wafer. We also found great potential of D. radiodurans IR SLP that apply in the nanobiotechnology.
We had demonstrate the capacity of D. radiodurans IR SLP self-assembled on native silicon wafer in low concentration hydrogen-bond-breaking agent which had added in a cell wall preparation to extract SLP. In optical microscopy (OM) and confocal laser scanning microscopy (confocal), we found self-assembled products with two structures on wafer:(1) Large-scale self-assembled film (large-scale SAF). (2) Multi-layered self-assembled film (multi-layered SAF). Atomic force microscopy (AFM) studies show that surface morphology of SAF was composed of uniform particles.
In applications, we used different buffer systems to directly synthesize Ag nanowire and variant Ag nanostructures on D. radiodurans IR SLP functionalized wafer. In improved systems, we can get dispersed Ag nanowire on wafer with average length=90 ± 6.7 μm (aspect ratio ~1000). In another system, we can adjust pH to fabricate variant Ag nanostructures on SAF.
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