臺灣博碩士論文加值系統

細菌表層蛋白（S-layers）為許多細菌及古生菌細胞最外層的組成結構。Deinococcus radiodurans具有複雜的細胞壁組成結構，其中包含S-layers。傳統上，製備方面通常都是利用化學藥劑處理方式將D. radiodurans的S-layers分離，效率不彰；應用性方面，S-layers通常以二維平面層次來利用。本篇論文想開發一種新的製程取得S-layers，並且探討S-layers在三維層次變化上的發展性。而我們成功的提供一種新穎簡單且自然的方法從D. radiodurans分離S-layers，我們稱為S-layers fraction (SLF)。更進一步，首先我們利用一種物理性力量使SLF形成許多中空顆粒 (SLFP)，直徑約100 – 200 nm。另外，我們在中性環境下將Ag+加至SLF溶液中。我們意外的發現一種中空球體結構，稱SLF-Ag，直徑約1 – 30 mm。這些結果顯示新的製程方式可有效的從D. radiodurans分離S-layers，此SLF能形成三維中空球體結構而具有利用性、可塑性、並且保有其原本在細胞上所扮演的生理角色。此外，SLF-Ag可能含有biogenic minerals，polarity，和evolution等意涵。

Bacterial cell surface layer proteins (S-layers) were the outermost cell envelope component of many bacteria and archaea. Deinococcus radiodurans, had a complex cell wall profile that including S-layers. Traditionally, in preparations, S-layers of D. radiodurans were usually isolated by chemical reagents treatment; in applications, S-layers were usually used in two-dimension form. We wanted to build up a method to efficiently isolate S-layers from D. radiodurans and research the development of S-layers in three-dimension form. We found that we succeeded in building up an easy and novel method to isolate the S-layers fraction (SLF). Furthermore, we firstly used physical force to make SLF to form particles (SLFP), diameter ~100 – 200 nm. Secondary, when Ag+ was added to SLF buffer solution at neutral condition, we unexpectedly found there were hollow spheres called SLF-Ag, the diameter ~1 – 30 mm. These results indicated that the novel method could efficiently isolate S-layers from D. radiodurans. SLF could form three-dimension hollow spheres and then they were useful, plasticity, and still owning inherent properties. Moreover, the meaning of the SLF-Ag may be about the implication of biogenic minerals, polarity, and evolution.

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