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研究生:郭廷威
研究生(外文):Kuo Ting-Wei
論文名稱:日本腦炎病毒封套蛋白第三區塊與單株抗體和肝素鍵結的結構研究
論文名稱(外文):Structural studies of the Domain III of Japanese encephalitis virus envelope protein binding with monoclonal antibody and Heparin.
指導教授:程家維
指導教授(外文):Cheng Jya-Wei
學位類別:碩士
校院名稱:國立清華大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
畢業學年度:92
語文別:中文
論文頁數:59
中文關鍵詞:日本腦炎病毒封套蛋白單株抗體肝素
外文關鍵詞:Japanese encephalitis virusenvelope proteinmonoclonal antibodyHeparin
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日本腦炎病毒分佈於整個亞洲,東至日本,西到印度。日本腦炎病毒封套蛋白在與細胞受體鍵結、免疫反應、病毒附著和低pH值融合進細胞的活姓中扮演重要的角色。
在我們應用核磁共振技術HSQC實驗的化學位移變化得到日本腦炎病毒封套蛋白Domain III對單株抗體E3.3的抗原決定位置後。我們應用四個單點突變的日本腦炎病毒封套蛋白Domain III,來進一步確認我們在HSQC實驗上所的到的結果。
我們也運用旋光儀來探討日本腦炎病毒封套蛋白Domain III的穩定性,其△G為5.15 kcal/mole。
在最近的研究中(11),日本腦炎封套蛋白261-402能夠與肝素鍵結,但是只有292-402時無法與肝素鍵結。我們已經進行了用肝素滴定日本腦炎封套蛋白Domain III(292-402)的實驗。 從HSQC實驗來看,並沒有明顯的化學位移發生,這樣的結果與最近的研究報告(11)相同。我們現在使用日本腦炎病毒封套蛋白261-420來進行與肝素的滴定實驗,企圖用NMR的技術來找到確切肝素與日本腦炎封套蛋白的鍵結位置。這一部份尚在進行中。
Japanese encephalitis virus (JEV) is found throughout Asia from India in the west to Japan in the east. JEV envelope protein plays an important role in cell receptor binding, immunological response, virion assembly and fusion activity at low pH state.
After we determining the epitope of JEV envelope protein Domain III binding with monoclonal antibody E3.3 by the chemical shift of NMR HSQC experiment. We used four site- directed mutant Domain III to confirm our result of HSQC experiment.
We also used circular dichroism to study the stability of JEV envelope protein Domain III. The △G of Domain III is 5.15 kcal/mole.
A recently study(11) shows that JEV envelope protein 261-402 can bind to Heparin, but only 292-402 can’t bind to Heparin. We already titrated Heparin to JEV envelope protein Domain III (292-402). By the HSQC experiment, there is no significant chemical shift in Heparin titrated to JEV envelope protein 292-402. This result is the same with the recently study(11). Now we use JEV envelope protein 261-420 to titrate with Heparin. Try to figure out the binding site of the JEV envelope protein by NMR. This part is still working.
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