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研究生:楊螢蓁
研究生(外文):Ying-Chen Yang
論文名稱:酵母菌中七萬道爾吞熱休克蛋白Ssa1p和Ssa2p甲基化之研究
論文名稱(外文):Methylation of Ssa1p and Ssa2p in Saccharomyces cerevisiae
指導教授:王群王群引用關係林彩雲林彩雲引用關係
指導教授(外文):Chung WangTsai-Yun Lin
學位類別:碩士
校院名稱:國立清華大學
系所名稱:生物資訊與結構生物研究所
學門:生命科學學門
學類:生物訊息學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:英文
論文頁數:41
中文關鍵詞:熱休克蛋白酵母菌甲基化
外文關鍵詞:Ssa1pSsa2pyeastMethylationmethyllysinemethylarginine
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蛋白質的後修飾作用(post-translational modification)包含了甲基化(methylation)、磷酸化(phosphorylation)…等。在生物體內某些蛋白分子之胺基酸在合成過程中可以被甲基化,如:組織胺酸(histidine)、離胺酸(lysine)和精胺酸(arginine),這些甲基化反應多半是不可逆的,但有可能是可被調控的,且往往在某些生理反應上扮演著重要的作用。在本論文中,我的實驗目的,是為了想去了解酵母菌(Saccharomyces cerevisiae)內分子量約略為七萬的熱休克蛋白(Ssa1p及Ssa2p)甲基化之狀況。我發現在正常的生長狀況下,熱休克蛋白(Ssa1p及Ssa2p)中某些離胺酸(lysine)和精胺酸(arginine)可以被甲基化。因此我試著想找出其甲基化的酵素(AdoMet-dependent methyltransferases),在一些實驗後,發現Ynl092wp可能是Ssa1p或Ssa2p的甲基化酵素(methyltransferases)。接著我利用雙突變菌種(double deletion strain)來探討這一課題。結果發現Ynl092wp並非Ssa1p的甲基化酵素。很不幸的,我們並沒有得到 ssa1Δynl092wΔ的雙突變種(double deletion strain),因此Ynl092wp是否為Ssa2p的甲基化酵素(methyltransferases)則不得而之。而Ssa1p和Ssa2p甲基化所扮演的作用亦不得而知,推測Ssa1p和Ssa2p的甲基化可能與生物體內的調節或是和反應基質(substrate)之間的相互作用力有著重要的關係。
Protein methylation is one of the post-translational modifications that may have important function. The basic amino acid residues such as histidine, lysine, and arginine can be methylated in vivo; and the methylation on basic amino acid appears irreversible and coupled to protein synthesis. In this study, I characterized the methylation of the 70-KDa heat shock proteins (Ssa1p and Ssa2p) in Saccharomyces cerevisiae. I showed that Ssa1p and Ssa2p were methylated in cells grown under normal conditions. The Ssa1p and Ssa2p are methylated on lysine as well as arginine residue(s). I also intended to identify the Ssa1p and Ssa2p-specific S-adenosylmethionine (AdoMet)-dependent methyl-transferases. On first trial, it appeared that Ynl092wp might be responsible for the methylation of Ssa1p or Ssa2p. Then, the double deletion strain was generated for further study. It is clear that Ynl092wp was not a Ssa1p-specific methyltransferase. However, the ssa1Δ ynl092wΔ double mutant was not obtained. Thus, it remains to be determined if Ynl092wp is a methyltransferase responsible for the methylation of Ssa2p. Moreover, biological functions of methylated Ssa1p and Ssa2p need to been determined. It might have important function in cell regulation or substrate interaction.
中文摘要 ………………………………..……………… i
謝誌 …………………………………....….………… ii
Abstract ………………………………....………… iii
Abbreviations …………………………………………… 1
Introduction ……….…………………….…………… 2
Materials and methods ………………………………. 7
Results ………..….…….……………………………. 12
Discussion ………....………………………………. 18
Tables …………….…………………….…………... 21
Figures ………….,…………………..…………….… 24
References ……………..………………………………. 37
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