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研究生:林佳永
論文名稱:胎鼠大腦神經元突觸之麩胺酸受器對鈣離子通透性研究
指導教授:張兗君
學位類別:碩士
校院名稱:國立清華大學
系所名稱:分子醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:39
中文關鍵詞:麩胺酸受器鈣離子電生理
外文關鍵詞:AMPA receptorsGluR2Ca2+ - permeable
相關次數:
  • 被引用被引用:0
  • 點閱點閱:111
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  • 下載下載:20
  • 收藏至我的研究室書目清單書目收藏:0
摘要


AMPA receptors在大腦中調控大部分快速的興奮性神經傳導物質,缺乏GluR2 的 AMPA receptors 會表現Ca2+ - permeable,這些受器會分布在大腦各個不同部位,有一些會表現在抑制性的 GABAergic interneurons 上。而在大腦皮質發育的早期,隨著發育的時期不同,也需要不同的AMPA receptors的活性。有些研究發現,在老鼠大腦發育早期大約出生後12到16天,AMPA receptors表現型為Ca2+ - permeable,但到了晚期 ( 出生16天之後 ) ,就為Ca2+ - impermeable。在這裡我們以胎鼠( E18 )取得大腦皮質神經細胞,進行離體無血清神經細胞培養,在培養第一週 ( 7DIV或8DIV ) 、第二週 ( 14DIV )時,以電生理的方法記錄突觸上麩胺酸受器的表現情形。我們利用high/low calcium交換實驗比較培養第一週 ( 7DIV或8DIV ) 與第二週 ( 14DIV ) 的神經元突觸上AMPA receptors對於Ca2+通透性表現的情形,結果發現,第一週的AMPA receptor不具有讓Ca2+通透的特性,而發育至第二週時,AMPA receptor亦不具有讓Ca2+通透的特性。顯示第一週及第二週時突觸上的AMPA receptor大多含有GluR2 subunit。
目 錄


壹、 緒論………………………………………1

貳、 實驗方法…………………………………8

參、 結果………………………………………16

肆、 討論………………………………………21

伍、 參考文獻…………………………………26

陸、 圖表集……………………………………31
伍、參考文獻


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