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研究生:林家漢
研究生(外文):Chia-Han Lin
論文名稱:豬關節軟骨中的蛋白質以及醣胺素之生化分析
論文名稱(外文):Biochemical analysis of porcine articular cartilage
指導教授:張兗君
指導教授(外文):Yen-Chung Chang
學位類別:碩士
校院名稱:國立清華大學
系所名稱:分子醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:70
中文關鍵詞:醣胺素軟骨蛋白體學二維電泳
外文關鍵詞:glycosaminoglycancartilageproteomics2D-PAGE
相關次數:
  • 被引用被引用:0
  • 點閱點閱:130
  • 評分評分:
  • 下載下載:15
  • 收藏至我的研究室書目清單書目收藏:0
我們使用豬的關節軟骨做為實驗模型,以不同溫度及時間對軟骨組織中的蛋白質以及醣胺素進行萃取,進而對萃取物中之蛋白質以及醣胺素成分進行定量及定性分析。

目前,我們發現將軟骨碎片冷凍乾燥後以 guanidine HCl 並加溫至60℃以上,可以快速萃取出大量以及完整之蛋白,目前已針對這些萃取蛋白進行蛋白體學之分析。正在進行中的工作包括:探討萃取醣胺素之最佳條件,以及建立分析醣胺素之方法學。預定在上述方法學建立完備之後,進而分析正常與病變之人類軟骨組織,以期對軟骨之各種組成的功能有更深入的了解,並能提供組織工程在人工軟骨研發上有用的資訊。
We use porcine articular cartilage as an experimental model to extract proteins and glycosaminoglycans to optimize the conditions for extraction of proteins and GAGs from cartilage and the procedures for carrying out biochemical analyses of the extractable materials.

Currently, we can extract sufficiently large amount of proteins after treating the lyophilized cartilage slices with guanidine HCl at
60℃, and we have conducted proteomic analysis of these extractable proteins. The work still in progress include: to optimize the extract conditions for glycosaminoglycans and to establish the methodology for glycosaminoglycans analysis. We will analyze normal and pathological human cartilage after establishing above methodologies in order to understanding the function of various cartilage components and to apply useful information for the tissue engineers to develop artificial cartilage.
中文摘要------------------------------------------i
英文摘要-----------------------------------------ii
謝誌--------------------------------------------iii
圖目錄--------------------------------------------v
表目錄------------------------------------------vii
附錄目錄---------------------------------------viii
壹、 引言-------------------------------------1
貳、 研究背景及目的---------------------------5
參、 材料與方法------------------------------10
肆、 結果與討論------------------------------26
伍、 未來展望--------------------------------41
陸、 參考文獻--------------------------------42
圖表集-------------------------------------------46
附錄---------------------------------------------64
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