臺灣博碩士論文加值系統

本試驗在比較三種純化之類胡蘿蔔素(蝦紅素，Astaxanthin、β-胡蘿蔔素，β-carotene、裸藻酮，Canthaxanthin )以及一種天然類胡蘿蔔素來源(綠藻粉，Chlorella meal)，添加於飼料中對於白棘三列海膽(Tripenustes gratilla)各成長指數、生殖腺成長以及色澤的影響。
實驗設計為4x2複因子試驗，採三重複進行。第一個因子為含5%綠藻粉與不含綠藻粉之兩個組別；第二個因子則是飼料中添加 10mg/100g純化之蝦紅素、β-胡蘿蔔素、裸藻酮以及未添加任何類胡蘿蔔素之控制組，合計八組飼料。實驗用白棘三列海膽平均體重117.43±13.11 g，平均殼徑長為69.14±5.15 mm，平均殼高為39.67±2.40 mm。每日餵食體濕重1%之飼料餵食，實驗共進行60天。
實驗結果顯示飼料中無論是添加純化之類胡蘿蔔素或是天然綠藻粉海膽各項成長指數如增重百分率(Percent weight gain, WTG)、飼料轉換係數(Feed conversion ratio, FCR)、蛋白質效率(Protein efficiency ratio, PER)與日成長率(Specific growth rate, SGR)皆無顯著差異。由飼料中添加各種純化類胡蘿蔔素之比較結果發現，以餵食添加β-胡蘿蔔素飼料之海膽其生殖腺指數(GI, 13.34)為最高，其次為餵食添加裸藻酮飼料之海膽(GI, 10.87)，再其次為餵食添加蝦紅素飼料之海膽(GI, 8.78)，餵食控制組飼料之海膽其生殖腺指數最低(GI, 8.16)；而飼料中添加5%天然綠藻粉對於生殖腺指數之提升並無助益。至於生殖腺色澤分析結果顯示飼料中添加純化之β-胡蘿蔔素者有最佳之色澤係數(1.90)，添加純化裸藻酮者次之(2.10)，添加蝦紅素者再次之(2.33)，控制組(2.90)最差；但是飼料中有否添加綠藻粉對生殖腺之色澤係數影響則不明顯。比較生殖腺色素含量分析結果中生殖腺β-胡蘿蔔素之含量高低後發現，添加純化β-胡蘿蔔素餵食之海膽其生殖腺中β-胡蘿蔔素含量為4.38 mg/100g，高於餵食添加純化裸藻酮飼料之海膽(2.40 mg/100g)以及餵食添加純化蝦紅素(2.25 mg/100g)飼料之海膽，而餵食控制組飼料之海膽其生殖腺中β-胡蘿蔔素含量最低(2.23 mg/100g)；至於飼料中綠藻粉的添加與否並不影響海膽生殖腺中β-胡蘿蔔素含量。

中文摘要-----------------------Ι
英文摘要---------------------ΙΙΙ
謝辭----------------------- V
目錄-----------------------VI
表目錄---------------------VII
一、前言----------------------1
二、文獻整理------------------3
三、材料與方法----------------12
四、結果-------------------23
五、討論-------------------30
六、結論-------------------40
七、參考文獻----------------41
附錄、英文縮寫檢索------------84

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