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研究生:王世弘
研究生(外文):Shi-Hong Wang
論文名稱:幾丁質與幾丁聚醣強化白蝦Litopenaeusvannamei免疫力及抵抗Vibrioalginolyticius感染之研究
論文名稱(外文):The immunostimulatory effects of chitin and chitosn on the white shrimp Litopenaeus vannamei and its resistaance against Vibrio alginolyticus
指導教授:陳建初陳建初引用關係
指導教授(外文):Jiann-Chu Chen
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:水產養殖學系
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:74
中文關鍵詞:白蝦幾丁質幾丁聚醣免疫因子吞噬作用清除能力
外文關鍵詞:litopenaeus vannameichitinchitosanimmune parametersphagocytic activityclearance efficiency
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摘要

本論文研究白蝦Litopenaeus vannamei經幾丁質(chitin)或幾丁聚醣(chitosan)注射處理後對其免疫因子(immune parameters)的影響,以及白蝦經幾丁質或幾丁聚醣注射處理後,增強對Vibrio alginolyticus感染抵抗力之研究。
白蝦注射6 μg g-1之幾丁質,經2天後,其總血球數明顯提高。白蝦注射6 μg g-1幾丁質後,經1天後,其酚氧化酵素活性與超氧離子產生明顯高於對照組。白蝦注射4 μg g-1與6 μg g-1幾丁質後,感染V. alginolyticus(2×105 cfu shrimp-1),經1天後,其吞噬作用及清除能力明顯高於對照組。白蝦注射4 μg g-1與6 μg g-1之幾丁質後,感染V. alginolyticus(2×106 cfu shrimp-1),然後放置於34 ‰之海水中,1天後,其活存率皆高於對照組及注射生理食鹽水組。證實白蝦經幾丁質處理下能提昇白蝦之免疫能力,以及增強對V. alginolyticus之扺抗力。
白蝦經注射4 μg g-1之幾丁聚醣,經1天後,總血球數明顯高於對照組,白蝦經注射2 μg g-1及4 μg g-1,經兩天後,其總血球數均高於對照組及注射生理食鹽水組。白蝦注射2 μg g-1及4 μg g-1之幾丁聚醣,經1天後,其酚氧化酵素活性、超氧離子產生及超氧岐化酵素活性明顯增加。白蝦經注射2 μg g-1及4 μg g-1之幾丁聚醣後,感染V. alginolyticus (2×106 cfu shrimp-1),然後放置於34 ‰海水中,經2天,其活存率明顯高於對照組及注射生理食鹽水組。白蝦注射2 μg g-1及4 μg g-1之幾丁聚醣後,感染V. alginolyticus,經1天,吞噬作用明顯增加。證實白蝦經幾丁聚醣處理下能提昇蝦之免疫能力,以及增加對V. alginolyticus之扺抗力。

關鍵字:白蝦、幾丁質、幾丁聚醣、Vibrio alginolyticus、免疫因子、吞噬作用、清除能力。
Abstract

This study examined the immune response of the white shrimp Litopenaeus vannamei, and its resistance against Vibrio alginolyticus when the shrimp were injected with chitin and chitosan.
L. vannamei injected with chitin at dose of 4 μg g-1 or 6 μg g-1 were examined for the immune parameters after 1, 2, 4 and 6 days. L. vannamei injected with chitin at a dose of 6 μl g-1 increased significantly its total haemocyte count (THC) after 2 days. L. vannamei injected with chitin at a dose of 6 μg g-1 increased significantly its phenoloxidase activity and respiratory burst after 1 day. L. vannamei injected with chitin at 4 μg g-1and 6 μg g-1 increased significantly its phagocytic activity and clearance efficiency against V. alginolyticus after 1 day. In another experiment, L. vannamei which had been injected with chitin, were challenged with V. alginolyticus at 2×106 colony-forming units (CFU) shrimp-1 and then placed in seawater of 34 ‰, the survival rate of shrimp that received chitin at dose of 4 μg g-1 and 6 μg g-1 after 1 day was significantly higher as compared to the shrimp that received saline and the control shrimp. It is therefore concluded that L. vannamei that received chitin at a dose of 4 μg g-1 increased its immune ability and resistance against V. alginolyticus infection.
L. vannamei injected with chitosan at dose of 2 μg g-1 or 4 μg g-1 were examined for the immune parameters after 1, 2, 4 and 6 days. L. vannamei injected with chitosan at 2 μg g-1 and 4 μg g-1 increased its total haemocyte count significantly as compared to the shrimp received saline and the control shrimp after 1 day. L. vannamei which was injected with chitosan at 2 μg g-1 and 4 μg g-1 increased significantly its phenoloxidase activity and respiratory burst after 1 day. L. vannamei injected chitosan at a dose of 4 μg g-1 significantly increased its phagocytic activity and clearance efficiency to V. alginolyticus after 2 days. In another experiment, L. vannamei which had been injected with chitosan, were challenged with V. alginolyticus at 2×106 CFU shrimp-1 and then placed in seawater of 34 ‰. The survival rate of shrimp that received chitosan at 2 μg g-1 and 4 μg g-1 after 2 days increased significantly as compared to the shrimp that received saline and the control shrimp. It is therefore concluded that L. vannamei that received chitosan at a dose of 2 μg g-1 increased its immune ability and resistance against V. alginolyticus infection.
The present study indicated that both chitin and chitosan can enchance the immune ability of L. vannamei. A continuous dose is necessary to maintain the immune ability of L. vannamei. Further research is needed to evaluate the immune ability of L. vannamei and its resistance against V. alginolyticus when shrimp fed a diet containing chitin or chitosan.

Key words: Litopenaeus vannamei, chitin, chitosan, Vibrio alginolyticus, immune parameters, challenge, phagocytic activity, clearance efficiency.
目錄
中文摘要..................................................................................................Ⅰ
英文摘要………………………………………………………………..Ⅲ
第一章 前言........................................................................................1
第二章 文獻整理................................................................................5
第三章 材料與方法..........................................................................23
3.1幾丁質與幾丁聚醣增強白蝦對於Vibrio alginolyticus感染抵抗力…………………………………………………………23
3.2 幾丁質與幾丁聚醣對白蝦免疫因子反應.............................26
3.3幾丁質與幾丁聚醣增強白蝦對於V. alginolyticus吞噬作用及清除能力………......................................................................30
第四章 結果......................................................................................32
4.1 幾丁質與幾丁聚醣增強白蝦對於V. alginolyticus感染抵抗   力………..................................................................................32
4.2 幾丁質與幾丁聚醣對白蝦免疫因子反應之影響.................33
4.3 幾丁質與幾丁聚醣增強白蝦對於V. alginolyticus吞噬作用及清除能力……………………………………………………..36
第五章 討論………………………………………………………..39
5.1 免疫刺激物對水產動物抵抗力之影響…………………….39
5.2 免疫刺激物對水產動物免疫因子反應影響.........................40
5.3 免疫刺激物對水產動物吞噬作用及清除能力之影響…….43
第六章 結論......................................................................................45
參考文獻..................................................................................................46
表..............................................................................................................64
圖..............................................................................................................66
附錄..........................................................................................................73
參考文獻

Allan, G. G., Fox, J. R., Kong, N., 1978. A critical evalution of the potential sources of chitin and chitosan. In : Muzzarelli, R. A. A., Pariser, E. R. (Eds.), Proceedings of the First International Coference on Chitin and Chitosan. MIT Sea Grant Program, Cambridge, Mass., pp. 64-78.
Anderson, D.P., 1992. Immunostimulant, adjuvants and vaccine carriers in fish: application to aquaculture. Ann. Rev. Fish Dis. 2, 281-307.
Anderson, D. P., Siwicki, A. K.,1994. Duration of protection against Aeromonas salmonicida in brook trout immunostimulated with glucan or chitosan by injection or immersion. Prog. Fish-Cult. 56, 258-261.
Anderson, R.S., 1996. Production of reactive oxygen intermediates haemocytes. Immunological significance. In : Söderhäll, K., Sadaaki, I., Vasta, G. (Eds), New Directions in Invertebrate Immunology. SOS Publications, Fair Haven. pp. 109-129.
Aoki, T., 1992. Chemotherapy and drug resistance in fish farms in Japan. In : Shariff, M., Subasighe, R. P., Arthur, J. R. (Eds.), Disease in Asian aquaculture 1. Fish Health Section, Asian Fisheries Society, Manila,Philippines, 519-529.
Austin, P. R., Brine, C. J., Castle, J. E. Zikakis, J. P., 1981. Chitin: new facts of research. Science 212 ,749-753.
Babior, B.M., Kipnes, R.S., Curnette, T., 1973. Biological defence system. The production by leukocytes of superoxide, a potential bactricidal agent. J. Clin. Invest. 52, 741-744.
Bachére, E., Mialhe, E., Rodríguez, J., 1995. Identification of defence parameters in the haemolymph of crustacean with particular reference to the shrimp Penaeus japonicus (Bate), prospects and application. Fish Shellfish Immunol. 5, 597- 612.
Barracco, M.A., Duvic, B., Söderhäll, K., 1991. The β-1,3-glucan binding protein from the crayfish Pacifastacus jeniusculus when reacted withβ-1,3-glucan, induces spreading and degranulation of crayfish granular cells. Cell Tissue Res. 266, 491-497.
Bauchau, A.G., 1981. Crustacean. In: Ratcliffe, N. A., Rowley, A. F. (Eds.), Invertebrate Blood Cell. Academic Press. pp. 387-417.
Bayne, C.J., 1990. Phagocytosis and non-self recognition in invertebrates. Phagocytosis appears to be an ancient line of defense. Bioscience 40, 723-731.
Bell, K.L., Smith, V.J., 1993. In vitro superoxide production by hyaline cells of the shore crab Carcinus maenas (L.). Dev. Comp. Immunol. 17, 211-219.
Campa-Córdrva, A.I., 1995. Diethyldithiocarbamate, a superoxidase dismutase inhibitor, counteracts the maturation of ischemic-like lesions caused by endothelin-1 intratriata injection. Neurosci. Lett. 190, 212-216.
Chen, J. Y., Pan, C. Y., Kuo, C. M., 2004. Molecular cloning and sequencing of shrimp (Penaeus monodon) penaeidin-5 cDNA. Fish Shellfish Immunol. 16, 665-670.
Cheng, W., Chen, J.C., 2001. Effects of intrinsic and extrinsic factors on the haemocyte profile of the prawn, Macrobrachium rosenbergii. Fish Shellfish Immunol. 11, 53-63.
Cheng, W., Liu, C.H., Yeh, S.T., Chen, J. C., 2004a.The immune stimulatory effect of sodium alginate on the white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus. Fish Shellfish Immunol. 10, 41-51.
Cheng, W., Liu, C. H., Yeh, S. T., Kuo, C. M., Chen, J. C., 2004b. Dietary administration of sodium enhances of white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus. Fish Shellfish Immunol.(in press)
Davis, J. F., Hayasaka, S. S., 1984. The enhancement of resistance of the American eel, Anguillar rostrata, to a pathogenic bacterium Aeromonas hydrophila, by an extract of the tunicate Ecteinascidia turbinate. J. Gen. Virol. 19: 125-127.
Destoumieux, D., Bulet, P., Loew, D., Van Dorsselaer, A., Rodriguez, J., Bachère, E., 1997. Penaeidins, a new family of antimicrobial petides isolated from the shrimp Penaeus vannamei (Decapoda). J. Biol. Chem. 272, 28398-28406.
Destoumieux, D., Bulet, P., Strub, J.M., Van Dorsselaer, A., Rodriguez, J., Bachère, E., 1999. Recombinant expression and range of activity of penaeidins, antimicrobial peptides from penaeid shrimp. Eur. J. Biochem. 266, 335-346.
Destoumieux, D., Saulnier, D., Garnier, J., Jouffrey, C., Bulet, P., Bachere, E., 2001. Crustacean immunity-antifungal peptides are generated from the c-terminus of shrimp hemocyanin in response to microbial challenge. J. Biol. Chem. 276, 47070-47077.
Di Guilio, R.T., Washburn, P.C., Wenning, R.J., Winston, G.W., Jewell, C. S., 1989. Biochemical responses in aquatic animals: a review of determinants of oxidative stress. Environ. Toxicol. Chem. 8, 1103-1123.
Duvic, B., Söderhäll, K., 1990. Purification and characterization of a β-1,3-glucan binding protein from plasma of the crayfish Pacifastacus leniusculus. J. Biol. Chem. 265, 9327- 9332.
Edahiro, T., Hamoguchi, M., Kusuda, R., 1990. Effects of glycyrrhizine against streptococcal infection of young yellowtail, Seriola quinqueradiata. Suisanzosoku 38: 239-243.
Esteban, M. A., Cuesta, A., Ortuno, J., Meseguer, J., 2001. Immunomodulatory effects of dietary intake of chitin on gilthead seabream (Sparus aurata L.) innate immune system. Fish Shellfish Immunol. 11, 303-315.
Esteban, M. A., Mulero, V., Cuesta, A., Ortuno, J., Meseguer, J., 2000. Effects of injecting chitin particles on the innate immune response of gilthead seabream (Sparus aurata L.). Fish Shellfish Immunol. 10, 543-554.
Fegan, D.F., 1998. The status of shrimp diseases in the APEC region, Aquaculture technical consultant, Alor Seetar, Kedah, Malaysia. In : Aquaculture forum, The 9th APEC FWG Meeting. Taipei, Chinese. 24.
Fox, C. J., 1993. The effect of dietary chitin on the growth, survival and chitinase levels in the digestive gland of juvenile Penaeus monodon (Fab.) Aquaculture 109, 39-49.
Ghidalia, W., Vengrely, R., Montmonty, C., Coirault, Y., Brouard, M. O., 1981. Coagulation in decapod crustacean. Comparative studies of the clotting process in species from group A, B and C. J. Comp. Physiol. 142, 473-478.
Hall, J.L., Rowlands, D.T., 1974. Heterogeneity of lobster agglutinins. II. Specificity of agglutinin-erythrocyte binding. Biochemistry 13, 828-832.
Hardie, L. J., Fletcher, T. C., Secombes, C. J., 1990. The effect of vitamin E on the immune response of Altanic salmon (Salmo salar). Aquaculture 87, 1-13.
Holmblad, T., Söderhäll, K., 1999. Cell adhesion molecules and antioxidative enzymes in a crustacean, possible role in immunity. Aquaculture 172, 111-123.
Hose, J.E., Martin, G.C., 1989. Defence functions of granulocytes in the ridgeback prawn Sicyonia ingentis. J. Invert. Pathol. 53, 335-346.
Hose, J.E., Martin, G.C., Gerard, A.S., 1990. A decapod hemocyte classification scheme integrating morphology, cytochemistry, and function. Biol. Bull. 178, 33-45.
Itami, T., Asano, M., Tokushige, K.,Kubono, K. Nakagawa, A., 1998. Enhancement of disease resistance of kuruma shrimp, Penaeus japonicus, after oral administration of peptidoglycan derived from Bifidobacterium thermophilum. Aquaculture, 164: 177-188.
Itami, T., Takahashi, Y., Tsuchihira, E., Igusa, H., Kondo, M., (1994) Enhancement of disease resistance of kuruma prawn Penaeus japonicus and increase in phagocytic activity of prawn hemocytes after oral adminisration of β- 1,3- glucan (Schizophyllan). In : Chou L.M., Munro, A.D., Lam, T. J., Chen, T.W., Cheong, L.K., Ding, J. K., Hooi, K. K. K., Phang, V. P. E., Shim, K. F., Tan, C. H. (Eds.), The Third Asian Fisheries Society, Manila, Philippines, pp 375- 378.
Jeuniaux, C., 1978. Distribution and quantitative importance of chitin in animals. In : Proceedings of the First international Conference on Chitin / Chitosan. By Muzzarelli, R. A. A., Pariser, E. R. (Eds.), MIT Sea Grant Program, Cambridge, Mass., pp.5-10.
Johansson, M.W., Söderhäll, K., 1985. Excytosis of the prophenoloxidase activating system from crayfish hemocytes. J. Comp. Physiol. 156, 175-181.
Johansson, M.W., Söderhäll, K., 1988. Isolation and purification of a cell adhesion factor from crayfish blood cells. J. Cell Biol. 106, 1795-1803.
Johansson, M.W., Söderhäll, K., 1989. A cell adhesion factor from crayfish hemocytes has degranulating activity towards crayfish granular cells. Insect Biochem. 2, 183-190.
Johansson, M.W., Keyser, P., Sritunyalucksana, K., Söderhäll, K., 2000. Crustacean haemocytes and haematopoiesis. Aquaculture 19, 45-52.
Johansson, M.W., Lind, M.I., Holmblad, T., Thörnqvist, P.-O., Söderhäll, K., 1995. Peroxinectin, a novel cell adhesion protein from crayfish blood. Biochem. Biophys. Res. Commun. 216, 1079- 1087.
Kajita, Y., Sakai, M., Atsuta, S., Kobayashi, M., 1990. The immunomodulation and adjuvant effects of levamisole on rainbow trout, Oncorhynchus mykiss. Fish Pathol. 25, 93-98.
Kang, C. J., Wang, J. X., Zhao, X. F., Yang, X. M., Shao, H. L., Xiang, J. H., 2004. Molecular cloning and expression analysis of Ch-penaeid, an antimicrobial peptide from Chinese shrimp, Fenneropenaeus chinensis. Fish Shellfish immunol. 16, 513-525.
Kawakami, H., Shinohara, N., Sakai, M., 1998. The non-specific immunostimulation and adjuvant effects of Vibrio anguillarum bacterin, M-glucan, chtin and Freund’s complete adjuvant against Pasteurella piscicida infection in yellowtail. Fish Pathol. 33, 287-292.
Kitao, T., Yoshida, T., 1986. Effect of an immunopotentiaor on Aeromonas salmonicida infection in rainbow trout (Salmo gairdneri). Vet. Immunol. Immunopathol. 12, 287-291.
Knorr, D., 1984. Use of chitinous polymer in food-A challenge for food research and development. Food Technol. 38: 85-97.
Kodama, H., Hirota, Y., Mukamoto, N., Baba, T., Azuma, I., 1993. Activation of rainbow trout (Oncorhynchus mykiss)phagocytes by muramyl dipeptide. Dev. Comp. Immunol. 17, 129-140.
Kono, M., Matsui, T., Shimizu, C., 1987. Effect of chitin, Chitosan and cellulose as diet supplements on the growth of cultured fish. Nippon Suisan Gakkaishi 53, 125-129.
Lee, K. K., Yu, S. R., Chen, F. R., Yang, T. Z., Liu, P. C., 1996. Virulence of Vibrio alginolyticus isolated from diseased tiger shrimp prawn Penaeus monodom. Curr. Microbiol. 33, 129-132.
Le Moullac, G., Le Groumellec, M., Ansquer, D., Froissard, S., Levy, P., Aquacop., 1997. Haemotological and phenoloxidase activity changes in the shrimp Penaeus stylirostris in relation with the moult cycle: protection against vibriosis. Fish Shellfish Immunol. 7, 227-234.
Liao, I. C., 1989. Penaeus monodon culture in Taiwan : Through two decades of growth. Int. J. Aq. Fish. Technol. 1, 16-24.
Lindsay, G. J. H., Walton, M. J., Adron, J. W., Flectcher, T. C., Cho, C. Y., and Cowey, C. B., 1984. The growth of the rainbow trout (Salmo Gairaneri) given diets containing chitin and its relationship to chitinolytic enzymes and chitin digestibility. Aquaculture 37, 315-334.
Lightner, D.V., Hedrick, R.P., Fryer, J.L., Chen, S.N., Liao, I.C., Kou, G.H., 1987. A survery of cultured penaeid shrimp in Taiwan for viral and other important diseases. Fish Pathol. 22, 127-140.
Liu, P.C., Lee, K.K., Yii, K.C., Kou, G.H., Chen, S.N., 1996. Isolation of Vibrio harveyi from diseased prawn Penaeus joponicus. Curr. Microbiol. 33, 129-132.
Liu, C. H., Yeh, S. T., Cheng, S. Y., Chen, J. C., 2004a. The immune response of the white shrimp Litopenaeus vannamei and its susceptibility to Vibrio infection in relation with the moult cycle. Fish Shellfish Immunol. 16, 151-161.
Liu, C. H., Cheng, W., Kuo, C. M., Chen, J. C., 2004b. Molecular cloning and characterisation of cell adhesion molecule, peroxinectin from the white shrimp Litopenaeus vannamei. Fish Shellfish Immunol. 17, 13-26.
Liu, C. H., Cheng, W., Hsu, J. P., Chen, J. C., 2004c. Vibrio alginolyticus infection in the white shrimp Litopenaeus vannamei confirmed by polymerase chain reaction and 16s rDNA sequencing. Dis. Aquat. Org. (in press).
Lo, C. F., Ho, C. H., Peng, S. E., Chen, C. H., Hsu, H. C., Chiu, Y. L., Chang, C. F., Liu, K. F., Su, M. S., Wang, C.H., Kou, G. H., 1996. White spot syndrome baculovirus (WSBV) detection in cultured and other arthropods. Dis. Aquat. Org. 27, 215-225.
Kobayashi, M., Johansson, M.W., Söderhäll, K., 1990. The 76kD adhension factor from crayfish hemocytes promotes encapsulation in vitro. Cell Tissue Res. 260, 13-18.
Kopacek, P., Gruhoffer, L., Söderhäll, K., 1993. Isolation and characterization of a hemagglutinin with affinity for lipopolysaccharides from plasma of crayfish Pacifastacus leniusculus. Dev. Comp. Immunol. 17, 407-418.
Maeda, M., Murakami, H., Ohta, H., Tajima, M., 1992. Stimulation of IgM production in human-human hybridoma HB4C5 cells by chitosan. Biosci. Biotechnol. Biochem. 56, 427-431.
Martin, G. G., Hose, J. E., Omori, S., Chong, C., Hoodbboy, T., McKrell, N., 1991. Localization and roles of coagulogen and transglutaminase in hemolymph coagulation in decapod crustaceans. Comp. Biochem. Physiol. 100 B, 517-522.
Merchie, G. E., Kontara, E., Lavens, P., Robles, R., Kurmaly, K., Sorgeloos, P., 1998. Effect of Vitamin C and astaxanthin on stress and disease resistance of postlarval tiger shrimp, Penaeus monodon (Fabricius). Aquacult. Res. 29, 579-585.
Minami, S., Suzuki, H., Okamoto, Y., Fujinaga, T., Shigemasa, Y., 1998. Chitin and chitosan active complement via the alternative pathway. Carbohydrate Polymers 36, 151-155.
Mix, M. C., Sparks, A. K., 1980. Hemocyte classification and differential counts in the dungenes crab, Cancer magister. J. Invert. Pathol. 35, 134-143.
Mori, K., Stewart, J. E., 1978. Natural and induced bactericidal activity of the hepatopancreas of the American lobster, Homarus americanus. J. Invert. Pathol. 32, 171-176.
Mourente, G., Rodriguez, A., 1997. Effects of salinity and dietary DHA (22:6n-3) content on lipid composition and performance of Penaeus kerathurus postlarvae. Mar. Biol. 128: 289-298.
Muzzarelli, R. A. A. Rocchetti, R., 1985. Deterination of the degree of acetylation of chitosan by first derivative ultraviolet spectrophotometry. Carbohyd. Polym. 5, 461-472.
Ninomiya, M., Hatta, H., Fujiki, M., Kim, M., Yamamoto, T., Kusuda, R., 1995. Enhancement of chemotactic activity of yellowtail (Seriola quinqueradiata) leucocytes by oral administration of quillaja saponin. Fish Shellfish Immunol. 5, 325-328.
Nishimura, K., Ishihara, C., Ueki, S., tokura, S., Azuma, I., 1986. Stimulation of cytokine production in mice using deacetylated chitin. Vaccine 2, 151-156.
Ogata, K., Muramoto, K., Yamazaki, M., Kamiya, H., 1983. Isolation and characterization of Balanus balanoides agglutinin. Bull. Jpn. Soc. Sci. Fish. 49, 1371-1375.
Persson, M., Vey, A., Söderhäll, K., 1987. Encapsulation of foreign particles in vitro by separated blood cells from crayfish, Astacus leptodactylus. Cell Tissue Res. 247, 409-415
Ravindranath, M.H., Paulson, J.C., 1988. O-acetyl-sialic acid specific lectin from the crab Cancer antennarius. Methods Enzymol. 138, 520-527.
Ratanapo, S., Chulavatnatol, M., 1990. Monodin, a new sialic acid-specific lectin from black tiger prawn (Penaeus monodon). Comp. Biochem. Physiol. 97 B, 515-520.
Ratcliffe, N.A., Rowley, A.F., Fitzgerald, S.W., Rhodes, C.P., 1985. Invertebrate immunity: basic concepts and recent advances. Internat. Rev. Cytol. 97, 183-350.
Robertsen, B., Rorstad, G., Engstad, R., Raa, J., 1990. Enhancement of non- specific disease resistance in Atlantic salmon, Salmo salar L., by a glucan from Saccharomyces cerevisiae cell walls. J. Fish Dis. 13, 391- 400.
Rodríguez, J., Le Moullac, G., 2000. State of the art of immunological tools and health control of penaeid shrimp. Aquaculture 191, 109-119.
Sakai, M., 1999. Current research status of fish immunostimulants. Aquaculture, 172:63-92.
Sakai, M., Kajita, Y., Kobayashi, M., Kawauchi, H., 1997. Immuostimulating effect of growth hormone: in vivo administration of growth hormone in rainbow trout enhances resistance to Vibrio anguillarum. Vet. Immunol. Immunopathol. 57, 147-152.
Sakai, M., Kobayashi, M., Kawauchi, H., 1996. In vitro activation of fish phagocytic cells by GH, prolactin and somatolactin. J. Endocrinol. 151, 113-118.
Sakai, M., Otubo, T., Atsuta, S., Kobayshi, M., 1993. Enhancement of resistance to bacterial infection in rainbow trout, Oncorhynchus mykiss (Walbaum) by oral administration of bovine lactoferrin. Fish Dis. 16, 239-247.
Sakai, M., Kamiya, H., Ishii, S., Atsuta, S., Kobayashi, M., 1992. The immunostimulating effects of chitin in rainbow trout, Oncorhynchus mykiss. In: Shariff, M., Subasighe, R. P., Arthur, J. R. (Eds.), Disease in Asian Aquaculture 1. Fish Health Section, Asuan Fisheries Society, Manlia, Philippines, 413-417.
Seidman, E.R., Lawrence, A.L., 1985. Growth, feed digestibility, and proximate body composition of juvenile Penaeus vannamei and Penaeus monodon grown at different dissolved oxygen levels. J. World Maricult. Soc. 16, 333- 346.
Shiau, S. Y., Yu, Y. P., 1998. Chitin but Not Chitosan Supplementation Enchances growth of Grass shrimp, Penaeus monodon. J. nutr. 128, 908-912.
Shiau, S. Y., Yu, Y. P., 1999. Dietary supplementation of chitin and chitosan depresses growth in tilapia, Oreochromis niloticus × O. aureus. Aquaculture 179, 439-446.
Shibata, Y., Forst, L. A., Metzger, W. J., Myrvik, Q. N., 1997. Alveolar macrophage priming by intravenous administration of chitin particles, polymer of N-acetyl-D-glucosamine, in mice. Infect. Immun. 65, 1734-1741.
Siwicki, A. K., Anderson, D. P., Rumsey, G, L., 1994. Dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against furunculosis. Vet. Immunol. Immunopathol. 41, 125-139.
Siwicki, A. K., Miyazaki, T., Komatsu, I., Matsuzato, T., 1996. In vitro influence of heat extract from firefly squid Watasenia scintillans on the phagocyte and lymphocyte activities in rainbow trout Oncorhynchus mykiss. Fish Pathol. 31, 1-7.
Smith, V.J., Johnston, P.A., 1992. Differential haemotoxic effect of PCB congeners in the common shrimp, Crangon crangon. Comp. Biochem. Physiol. 101 C. 641-649.
Smith, V.J., Söderhäll, K., 1983. β-1,3-glucan activation of crustacean hemocytes in vitro and in vivo. Biol. Bull. 164, 299-314.
Söderhäll, K., Cerenius, L., 1992. Crustacean immunity. Ann. Rev. Fish Dis. 3-23.
Söderhäll, K., Cerenius, L., 1998. Role of the prophenoloxidase activating system in invertebrate immunity. Curr. Opin. Immunol. 10, 23-28.
Söderhäll, K., Smith, V. J., 1983. Separation of the haemocyte populations of Carcinus maenas and other marine decapoda, and prophenoloxidase distribution. Dev. Comp. Immunol. 7, 229-239.
Söderhäll, K., Aspan, A., Duvic, B., 1990. The proPO system and associated proteins; role in cellular communication in arthropods. Res. Immunol. 141, 896-907.
Söderhäll, K., Smith, V.J., Johansson, M.W., 1986. Excytosis and uptake of bacteria by isolated hemocyte populations of two crustacean: Evidance for cellular cooperation in the defense reactions of arthropods. Cell Tissue Res. 245, 43-49.
Song, Y.L., Hsieh, Y.T., 1994. Immunostimulation of tiger shrimp (Penaeus monodon) hemocytes for generation of microbicidal substances: analysis of reactive oxygen species. Dev. Comp. Immunol. 18, 201-209.
Song, Y.L., Cheng, W., Wang, C.H., 1993. Isolation and characterization of Vibrio damsela infectious for cultured shrimp in Taiwan. J. Invert. Pathol. 61, 24-31.
Sritunyalucksana, K., Söderhäll, K., 2000. The proPO and clotting system in crustaceans. Aquaculture. 191,53-69.
Sritunyalucksana, K., Wongsuebsantati, K., Johansson, M. W., Söderhäll, K., 2001. Peroxinectin, a cell adhesive protein associated with the proPO system from the black tiger shrimp, Penaeus monodom. Dev. Comp. Immunol. 25,353-63.
Stewart, J.E., Foley, E.M., 1969. A precipitin reaction of the hemolymph of the lobster Homarus americanus. J. Fish Res. Bd. Can. 26, 1392-1397.
Stewart, J.E., Zwicker, B.M., 1972. Natural and induced bactericidal activities of the lobster, Homarus americanus: products of hemocyte-plasma interaction. Can. J. Microbiol. 18, 1499-1509.
Sung, H. H., Kou, G. H., Song, Y. L., 1994. Vibriosis resistance induced by glucan treatment in tiger shrimp (Penaeus monodon). Fish Pathol. 29, 11-17.
Sung, H. H., Yang, Y. L. Song, Y. L., 1996. Enhancement of microbicidal activity in the tiger shrimp Penause monodom via immunostimulation. J. Crustacean. Biol. 16, 278-284.
Suzuki, K., Okawa, Y., Hashimoto, K., Suzuki, S., Suzuki, M., 1984. Protecting effect of chitin and chitosan on experimentally induced murine candidiasis. Microbiol. Immunol. 28: 902-912.
Suzuki, Y., Ai, T., 1989. IHN resistance with megalevel doses ascorbic acid in rainbow trout, Salmo gairaneri fry. Bull. Shizuoka Pref. Fish. Exp. Stn. 24: 5-29, (In Japanese).
Takahashi, Y., Itami, T., Kondo, M., 1995. Immunodefense system of Crustacea. Fish Pathol. 30, 141-150.
Takahashi, Y., Kondo, M., Itami, T., Honda, T., Inagawa, H., Nishizawa, T., Soma, G.I., Yokomizo, Y., 2000. Enhancement of disease resistance against penaeid acute viraemia and induction of virus-inactivating activity in haemolymph of kuruma shrimp, Penaeus japonicus, by oral administration of pantoea agglomerans lipopolysaccharide(LPS). Fish Shellfish Immunol. 10, 555- 558.
Tamai, T., Shirahata, S., Noguchi, T., Sato, N., Kimura, S., Murakami, H., 1993. Cloning and expression of flatfish (Paralichthys loivaceus) interferon cDNA. Biochem. Biophys. Acta 1174, 182-186.
Tanigawa, T., Tanaka, Y., Tomita, K., Sasaki, T., Sashiwa, H., Saimoto, H., Shigemasa, Y., Okamoto, Y., Minami, S., Matsuhashi, A., 1992. Effect of chitin on the production of interleukin-1b in human blood monocytes. Yonago Acta Medica 35, 147-150.
Thörnqvist, P.-O., Johansson, M.W., Söderhäll, K., 1994. Opsonic activity of cell adhesion protein and β-1,3-glucan binding protein from two crustaceans. Dev. Comp. Immunol. 18, 3-12.
Truscott, R., White, K.N., 1990. The influence of metal and temperature stress on the immune system of crabs. Funct. Ecol. 4, 455-461.
Vasta, G.R., Warr, G.W., Marchalonis, J.J., 1983. Serological characterization of humoral lectins from the freshwater prawn Macrobrachium rosenbergii. Dev. Comp. Immunol. 7, 13- 20.
Vargas-Albores, F., Jiméne-Vega, F., Söderhäll, K., 1996. A plasma protein isolated from brown shrimp ( Penaeus californiensis) which enchances the activation of prophenoloxidase system by β-1,3-glucan. Dev. Comp. Immunol. 20, 299-306.
Vargas-Albores, F., Jiméne-Vega, F., Yepiz-Plascencia. G., 1997. Purification and comparisom of β-1,3-glucan binding protein from white shrimp (Penaeus vannamei). Comp. Biochem. Physiol. 116B, 453-458.
Yano, T., Mangindaan, R.E.P., Matsuyama, H., 1989. Enhancement of the resistance of carp Cyprinus carpio to experimental Edwardsiella tarda infection, by some β-1,3- glucan. Nippon Suisan Gakkaishi 55, 1049- 1054.
Yoshida, T., Kruger, R., Inglis, V., 1995. Augmentation of non-specific protection in African catfish, Clarias gaiepinus (Burchell), by the long-term oral admininstration of immunostimulants. Fish Dis. 18, 195-198.
Yu, C. I., Song, Y. L., 2000. Outbreaks of Taura syndrome in Pacific white shrimp Penaeus vannamei culture in Taiwan. Fish Pathol. 35, 21-4.
王偉、秦汶、李素清、薄淑琴,1991。甲殼素的分子量。應用化學,8: 85-87。
中華民國台灣地區漁業年報,2003。行政院農業委員會漁業署。
江晃榮,1998。生體高分子(幾丁質、膠原蛋白)產業現況與展望。財團法人生物技術開發中心,pp 1-99。
宋延齡、黃志成,1999。應用免疫賦活劑以防治蝦類疾病。生物產業10:159-169.
林俊煌,1992,不同去乙醯程度之幾丁聚醣的流變性質與鍊柔軟度、膜之物理特性的關係。國立台灣海洋大學食品科學研究所碩士論文
張正芳、蘇茂森、陳宏遠、廖一久,1996。由Schizophyllum commune 萃取之多醣類β-1,3-glucan與多聚磷酸態維生素C ( Polyphosphorylated L-ascorbic Acid )對強化草蝦抵抗弧菌與受傷組織復原能力之研究。水產研究,4 : 43-54。
張錦宜,李武忠,陳淑玲,徐崇仁,1997。飼料中添加幾丁質對斑節蝦成長及抗菌活性之影響。水產研究,5:11-19。
張錦宜,徐崇仁,1999。飼料中添加幾丁質對草蝦免疫活性之影響。行政院農委會 水產試驗所-潮訊,131:3-6。
陳逢叡,1993。Vibrio damsela對草蝦的致病性研究。國立台灣海洋大學生物研究所碩士論文.
楊佳宏,2002。應用幾丁質與幾丁聚醣強化草蝦抵抗白點桿狀病毒及弧菌感染之研究。國立中山大學海洋生物研究所碩士在職專班碩士論文。
賴淑琪,1999。病原性弧菌其細胞外產物與石斑魚血清成分之交互作用。國立台灣海洋大學水產養殖所碩士論文。
劉瓊淑,1994。幾丁質,幾丁聚醣及其相關酵素之特性與應用。食品工業,26: 26-36。
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