臺灣博碩士論文加值系統

本研究目的主要是探討Gluconacetobacter xylinum生成細菌纖維素之生產條件與細菌纖維素之物理性質，以作為生物醫學材料可行性之評估。物理性質的探討包含了細菌纖維素的復水性、拉張力、透光度、水解度、蛋白質沈積性、水氣透過率與氧氣透過率。
當培養液初始pH為6.0時細菌纖維素的產生速度最快，所以可知產生細菌纖維素的最適初始pH值為6.0左右。靜置培養前期產生細菌纖維素的速度比震盪培養快，但以總生產量來說，震盪培養產生之細菌纖維素量會多於靜置培養。面積越大的培養液生成的細菌纖維素會越多，但會造成細菌纖維素薄膜的表面較不平滑、粗糙。
細菌纖維素的保水力約為200 %左右，其復水能力為冷凍乾燥（170 %）＞30 ℃通風乾燥（130 %）＞通風乾燥70 ℃（110 %）；蛋白質吸附量會因結構越緊密吸附量越多；細菌纖維素在14天重量損失約2.8 %，具有良好的水解穩定度；孔隙越大的細菌纖維素則水氣透過率與氧氣通透率越高；隨著細菌纖維素薄膜的厚度增加，其透光度會下降；當細菌纖維素水含量越高時，其拉張強度越小；乾燥的細菌纖維素具有相當高的拉張強度，當水含量上升時，細菌纖維素的拉張強度會有下降的現象，而氧氣通透率卻上升，反之，當水含量下降時，兩者有相反之結果。
細菌纖維素必須保存於中性水溶液之下，才能維持其細緻的網狀結構。

中文摘要……………………………………………………….……..I
英文摘要………………………………………………………..……II
一、前言……………………………………………………………...1
二、文獻整理………………………………………………………..4
1. 細菌纖維素…..……………………………………………...4
1.1. 細菌纖維素生合成之代謝途徑………………………..4
1.2. 細菌纖維素之形成……………………………………..5
2. 醋酸菌 Acetobater xylinum…………………………………9
2.1. A. xylinum之型態………………………………...……..9
2.2. A. xylinum生產細菌纖維素之培養特性……...............10
2.3. A. xylinum生產細菌纖維素之調節作用…...................10
2.4. 影響生成細菌纖維素醋酸菌生長之因子……………11
2.4.1. pH值…...………………………………………...11
2.4.2. 溫度……………………………………...………11
2.4.3. 溶氧量…………………………………………...15
2.4.4. 振盪與靜置培養……………………………...…15
2.5. A. xylinum菌種保存方法……………...………………15
3. 細菌纖維素的化學成分及其物理性質…...…………...….16
3.1. 細菌纖維素的化學成分…...…………...…………......16
3.2. 細菌纖維素之微組織結構…...…………...…………..16
3.3. 細菌纖維素之一般特性及與植物纖維之差異………16
3.4. 細菌纖維素的性質……………………………………17
3.5. 細菌纖維素品質上的修飾……………………………18
4. 細菌纖維素之特殊性質…………………………………...18
4.1. 超微細網狀結構（Ultrafine network structure）…….18
4.2. 親水能力（Hydrophilicity）………………………….19
4.3. 透明度（Transparency）………………………………19
5. 細菌纖維素的應用………………………………………...19
6. 水膠（Hydrogel）之定義、種類與應用方法……………25
6.1. 水膠的定義及分類……………………………………25
6.2. 生醫上的應用…………………………………………25
6.3. 水在水膠中所扮演的角色……………………………26
6.4. 含水量…………………………………………………28
6.5. 抗蛋白沈積……………………………………………29
6.6. 機械強度………………………………………………29
6.7. 水解穩定度……………………………………………30
三、材料與方法…………………………………………………….31
1. 實驗流程…………………………………………………...31
2. 實驗材料與儀器…………………………………………...32
2.1. 實驗使用菌株…………………………………………32
2.2. 菌株培養基……………………………………………32
2.3. 化學藥品………………………………………………33
2.4. 儀器設備………………………………………………33
3. 實驗方法…………………………………………………...34
3.1. 菌種活化………………………………………………34
3.2. 菌種培養………………………………………………35
3.2.1. 不同pH值………………………………………35
3.2.2. 靜置培養與震盪培養…………………………...35
3.2.3. 不同表面積……………………………………...35
3.3. 細菌纖維素薄膜保存…………………………………35
3.4. 厚度測定………………………………………………36
3.5. 機械性質測試…………………………………………36
3.6. 水含量測定……………………………………………36
3.7. 水氣透過測定…………………………………………37
3.8. 抗蛋白質沈積試驗……………………………………37
3.9. 水解穩定度測試………………………………………38
3.10. 氧氣通透性測試……………………………………..38
3.11. 透光度之測定………………………………………..40
3.12. 掃瞄式電子顯微鏡（SEM）之型態觀察…………..41
四、結果與討論……………………………………………………42
五、結論……………………………………………………………48
六、參考文獻...…………………………………………………….50
圖……………...…………………………………………………….55
表……………...…………………………………………………….72

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