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研究生:陳播暉
研究生(外文):Po-Hui Chen
論文名稱:幾丁聚醣/果膠複合材料之製備與特性探討
論文名稱(外文):Preparation and Characterization of Chitosan/Pectin Composite Materials
指導教授:謝學真
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:化學工程學研究所
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:120
中文關鍵詞:果膠幾丁聚醣
外文關鍵詞:pectinchitosan
相關次數:
  • 被引用被引用:3
  • 點閱點閱:346
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本研究針對幾丁聚醣(chitosan)與果膠(pectin)這兩種天然的多醣類高分子進行複合材料的研究。由於幾丁聚醣分子帶正電而果膠帶負電,故兩種分子能以離子鍵的作用方式形成離子型複合材料。另一作用方式則是在離子鍵之外,再透過以EDC及NHS的交聯作用使兩種分子之間還有共價鍵的形成,稱之為離子與共價型複合材料。從機械性質測定的結果顯示,離子型複合基質在果膠比例提高的情況下,其強度從0.05 MPa上升一倍至0.12 MPa左右。另外在離子與共價型複合基質方面,隨著交聯劑濃度的提高,複合基質在強度上從0.08 MPa上升至0.12 MPa左右,但在延展性方面卻不受影響。另外在離子型複合材料方面,當果膠比例提高時,複合材料之親水性也隨之上升,而在離子與共價型複合材料方面,隨著交聯劑濃度上升,複合材料之親水性也跟著變佳。從DSC的結果,在離子型或離子與共價型複合材料,其兩種物質之間都因為內部作用力的增強,使其熱性質發生變化,Tg值上升。果膠的添加亦增加了幾丁聚醣之降解性與細胞相容性。本研究成功的以幾丁聚醣與果膠這兩種多醣類高分子,以離子鍵或離子與共價鍵的方式進行複合,開發出新型的複合材料,此一複合材料具有強度較高、親水性佳、細胞相容性較優等特點,故它在生醫材料及其相關領域具有其應用的潛力和價值。
In this research, chitosan and pectin, two kinds of natural polysaccharide were used to form composite materials. Because chitosan is poly-cationic and pectin is poly-anionic, the ionic interaction between these two polymers form polyelectrolyte. Besides, another kind of composite material was formed by not only the ionic interaction, but also the covalent bond between two polymers by the addition of the cross-linking agents—EDC & NHS. The mechanical tests indicated that with the increase of the ratio of pectin/chitosan, the mechanical strength of composite material formed by the ionic interaction increased from 0.05 MPa to about 0.12 MPa. Besides, the mechanical strength of the composite material formed by the (ionic and covalent) bond increased from 0.08 MPa to 0.12 MPa, but the strain at max. load didn’t decrease. The measurement of contact angles revealed that with the increase of the ratio of pectin/chitosan, the hydrophilicity of the composite materials increased. With the increase in the crosslinking agent concentration, the hydrophilibility of the composite materials increased, too. The DSC analysis showed that the interactions between two the polymers increased the values of Tg(glass transition temperature). The rate of degradation and the biocompatibility of chitosan were also enhanced by the addition of pectin. In this research, new composite materials were successfully made of chitosan and pectin using either ionic only or (ionic and covalent bond)interactions. Because of the good mechanical strength, high hydrophilicity, and excellent cytocompatibility, these composite materials have application potential in biomaterial-related fields.
誌謝 I
中文摘要 III
英文摘要 V
目錄 VII
圖目錄 XI
表目錄 XIV
縮寫與符號說明 XV
中英名詞對照 XVII

第一章 緒論
1.1 研究背景 1
1.2 幾丁聚醣 3
1.2.1 幾丁聚醣之性質 3
1.2.2 幾丁聚醣之應用 5
1.2.2.1 生醫材料方面之應用 5
1.2.2.2 其他方面之應用 6
1.3 果膠 7
1.3.1 果膠之性質 7
1.3.2 果膠之應用 11
1.4 複合材料 12
1.5 高分子基質製備 23
1.6 研究動機 25
1.7 研究架構 28

第二章 實驗藥品、儀器與方法
2.1 實驗藥品 33
2.1.1 混合溶液製備 33
2.1.2 多孔狀複合基質製備 34
2.1.3 複合基質之材料性質測定 34
2.1.3.1 複合材料之細胞相容性測定 34
2.2 實驗儀器 35
2.2.1一般儀器 35
2.2.2 混合溶液製備 36
2.2.2.1混合溶液之黏度測定 36
2.2.3 多孔狀複合基質製備 37
2.2.4複合基質之材料性質測定 37
2.2.4.1 複合基質之結構觀察 37
2.2.4.2 複合基質之機械性質測定 38
2.2.4.3 複合材料之親水性測定 38
2.2.4.4 複合材料之熱性質分析 38
2.2.4.5 複合基質之吸水能力測定 39
2.2.4.6 複合基質之降解性測定 39
2.2.4.7 複合基質之細胞相容性測定 40
2.3 實驗方法 41
2.3.1 混合溶液製備 41
2.3.1.1 離子型鍵結混合液製備 41
2.3.1.2 離子與共價型交聯混合液製備 42
2.3.1.3 混合溶液之黏度測定 43
2.3.2 多孔狀複合基質製備 44
2.3.3 複合材料之性質測定 45
2.3.3.1 複合基質之結構觀察 45
2.3.3.2 複合基質之機械性質測定 46
2.3.3.3複合材料之親水性測定 47
2.3.3.4複合材料之熱性質分析 49
2.3.3.5複合材料之吸水能力測定 50
2.3.3.6複合材料之降解性分析 51
2.3.3.7複合材料之細胞相容性測定 52

第三章 結果與討論
3.1 混合溶液製備 57
3.2 多孔狀複合基質製備 60
3.3 複合材料之性質測定 60
3.3.1複合基質之孔洞結構觀察 60
3.3.2複合基質之機械性質測定 72
3.3.2.1離子型複合基質之機械性質 72
3.3.2.2 離子與共價型複合基質之機械性質 76
3.3.3複合材料之親水性測定 80
3.3.3.1離子型複合材料之親水性 80
3.3.3.2 離子與共價型複合材料之親水性 83
3.3.4複合材料之熱性質分析 86
3.3.4.1離子型複合材料之熱性質分析結果 86
3.3.4.2 離子與共價型複合材料之熱性質分析結果 88
3.3.5複合基質之吸水能力 90
3.3.5.1離子型複合基質之吸水能力 90
3.3.5.2 離子與共價型複合基質之吸水能力 92
3.3.6複合基質之降解性 94
3.3.6.1離子型複合基質之降解性 94
3.3.6.2 離子與共價型複合基質之降解性 98
3.3.7複合材料之細胞相容性測定 100

第四章 結論
4.1 結論 109
4.2 未來研究方向 111

參考文獻 113
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