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研究生:林安婷
研究生(外文):An-Ting Lin
論文名稱:蝦白點症病毒潛伏性相關基因之鑑定
論文名稱(外文):Identification of white spot syndrome virus latency-associated genes
指導教授:郭光雄羅竹芳羅竹芳引用關係
指導教授(外文):Guang-Hsiung KouChu-Fang Lo
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:動物學研究研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:61
中文關鍵詞:蝦白點症病毒潛伏感染
外文關鍵詞:latencyWSSV
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近年來,在一般草蝦養殖池之草蝦白點症病毒檢測結果顯示,約90%為白點症病毒帶原蝦。潛伏性病毒感染屬於一種病毒與寄主長期共存的關係,病毒潛伏狀態的維持與少數病毒基因之表現有關。本研究目的在於鑑定並確定蝦白點症病毒潛伏性相關基因的存在。針對白點症病毒532個開放譯讀區進行反轉錄酶-聚合酶鏈反應,發現在白點症病毒潛伏感染時期,草蝦體內確實有白點症病毒基因wssv194、wssv313與wssv369轉錄表現,其時序表現量呈現漸增之現象。而蛋白質序列預測分析顯示,此三個白點症病毒基因產物包含多種基因調節蛋白質之弁鄔夆炾魽A推測其可能參與病毒於潛伏時期的基因轉錄調控。
For recent years, the detection of white spot syndrome virus (WSSV) of general cultured black tiger shrimps in Taiwan reveals that as high as 90% shrimps are WSSV carriers. Latent viral infection generates a long-term relationship between virus and its host. Under the latent state, few viral genes are expressed, which establishes the latency stage. The study examines the transcriptional expression of WSSV 532 predictive open reading frames and further identifies the WSSV latent-associated genes by the reverse transcriptase- DNA polymerase chain reaction. It was found that wssv194, wssv313 and wssv369 express in the WSSV carried Penaues monodom, and the expression level increases with the infection time. The protein sequence analysis reveals that some predictive functional motifs of gene regulatory protein exist in the WSSV latency-associated genes. These three latency-associated genes might possibly be involved in the transcriptional regulation of WSSV latent stage.
前言…1
材料與方法…10
一、 人工感染試驗與草蝦檢體之收取…10
二、 檢體DNA之萃取與白點症病毒檢測…10
三、 檢體RNA之萃取…11
四、 反轉錄酶-聚合酶鏈反應…12
五、 快速增殖cDNA末端…13
1. 5’端(轉錄起始點)快速增殖cDNA末端…13
2. 3’端快速增殖cDNA末端…15
結果…16
蝦白點症病毒潛伏性感染相關基因之轉錄表現…16
蝦白點症病毒潛伏性感染相關基因之轉錄本分析…16
蝦白點症病毒潛伏性感染相關基因之胺基酸序列分析…18
討論…21
蝦白點症病毒潛伏性感染相關基因之轉錄表現…21
蝦白點症病毒潛伏性感染相關基因之轉錄調控探討…23
蝦白點症病毒潛伏性感染相關基因弁鈺敦Q…28
結論…32
參考文獻…33
圖表…48
附錄…60
附錄一、潛伏性相關基因於不同病毒分離株之ORF對照表…60
附錄二、本論文所使用之引子序列一覽表…61
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