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研究生(外文):Yi-Chun Chen
論文名稱(外文):The Effect of Epstein-Barr Virus Protein Kinase BGLF4 on Cellular DNA Repair Activity
外文關鍵詞:EBV protein kinase BGLF4
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細胞內的蛋白質激酶在訊息傳導、細胞週期的調控等弁鄐W扮演著重要的角色,而病毒蛋白質激酶除了可以調節病毒的複製週期外,也可能透過磷酸化宿主蛋白質,影響細胞蛋白質的弁遄CEB病毒的BGLF4是EB病毒唯一已知的Ser/Thr蛋白質激酶(protein kinase),屬於溶解期早期表現的基因產物,BGLF4有自我磷酸化的現象,並有磷酸化EA-D、EF-1δ和ganciclovir的能力。在本篇研究中,以酵母菌雙雜交(yeast two hybrid)的方式篩選帶有EB病毒的類淋巴球母細胞株互補基因庫,發現BGLF4可分別與基因體核苷酸切除修復中負責辨認錯誤DNA的蛋白質XPC (xeroderma pigmentosum complementation group C),和腫瘤抑制因子BRCA1的共同抑制因子ZBRK1 (Zinc finger and BRCA1-interacting protein with a KRAB domain 1) 產生交互作用。因為這兩個蛋白質都參與細胞內DNA修復機制,故進一步探討BGLF4與XPC之間可能的交互作用,同時也研究BGLF4對細胞DNA修復能力可能的影響。首先利用共同免疫沉澱法證實無激酶活性的BGLF4突變蛋白質和HA tagged XPC蛋白質片段的交互作用。以免疫螢光染色觀察BGLF4與XPC在HeLa細胞內表現的位置,發現兩蛋白質在細胞內位置有部分重疊。在宿主細胞質體再活化試驗中,發現BGLF4可以促進p53缺失的H1299/bcl2細胞修復受損DNA的能力,而在XPC突變的XP4PA-SV細胞中則無此現象。至於BGLF4促進細胞修復DNA的能力是否經由需要XPC的機制,或是藉由其他的途徑,仍需進一步的研究。
Cellular protein kinases are known to be involved in regulating many cellular events such as signal transduction or cell cycle progression. Viral protein kinases are expected to play important roles in modulating not only viral replication machinery but also the biological function of infected cells. BGLF4, which expresses as an Epstein-Barr virus (EBV) early gene, is the only identified Ser/Thr protein kinase of EBV. The kinase activities of BGLF4 were demonstrated for autophosphorylation and phosphorylating viral DNA polymerase accessory factor EA-D, cellular translation elongation factor EF-1δ and ganciclovir. To identify possible cellular substrates of BGLF4, yeast two-hybrid system was employed to screen a cDNA library derived from an EBV positive lymphoblastoid cell line in this study. Two cellular proteins identified were global genome nucleotide excision repair initiator XPC (xeroderma pigmentosum complementation group C) and ZBRK1 (Zinc finger and BRCA1-interacting protein with a KRAB domain 1, which was implicated as a corepressor of BRCA1). Since both proteins are involved in the DNA repair system, the interaction between BGLF4 and XPC, and the possible effect of BGLF4 on cellular DNA repair machinery were further analyzed. The interaction between a kinase dead BGLF4 mutant and an HA tagged XPC truncated protein were confirmed by co-immuno- precipitation with reciprocal antibodies. BGLF4 and XPC were found partially colocalized in HeLa cells as demonstrated by immunofluorescence. In host cell reactivation assay, BGLF4 was found to enhance the DNA repair activity in H1299/bcl2 cell that harbors defective p53, but not in XPC mutanted XP4PA-SV cell. Whether BGLF4 enhances the DNA repair activity through an XPC dependent mechanism or other pathway needs to be further investigated.
中文摘要 -Ⅰ-
英文摘要 -Ⅱ-
前言 01
材料與方法 14
結果 23
討論 28
圖表 33
參考文獻 50
Adams, A. & Lindahl, T. (1975). Epstein-Barr virus genomes with properties of circular DNA molecules in carrier cells. Proc Natl Acad Sci U S A 72, 1477-81.
Ansari, A. & Emery, V. C. (1999). The U69 gene of human herpesvirus 6 encodes a protein kinase which can confer ganciclovir sensitivity to baculoviruses. J Virol 73, 3284-91.
Baek, M. C., Krosky, P. M., Pearson, A. & Coen, D. M. (2004). Phosphorylation of the RNA polymerase II carboxyl-terminal domain in human cytomegalovirus-infected cells and in vitro by the viral UL97 protein kinase. Virology 324, 184-93.
Baer, R., Bankier, A. T., Biggin, M. D., Deininger, P. L., Farrell, P. J., Gibson, T. J., Hatfull, G., Hudson, G. S., Satchwell, S. C., Seguin, C. & et al. (1984). DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature 310, 207-11.
Berwick, M. & Vineis, P. (2000). Markers of DNA repair and susceptibility to cancer in humans: an epidemiologic review. J Natl Cancer Inst 92, 874-97.
Bonnet, M., Guinebretiere, J. M., Kremmer, E., Grunewald, V., Benhamou, E., Contesso, G. & Joab, I. (1999). Detection of Epstein-Barr virus in invasive breast cancers. J Natl Cancer Inst 91, 1376-81.
Bruni, R., Fineschi, B., Ogle, W. O. & Roizman, B. (1999). A novel cellular protein, p60, interacting with both herpes simplex virus 1 regulatory proteins ICP22 and ICP0 is modified in a cell-type-specific manner and Is recruited to the nucleus after infection. J Virol 73, 3810-7.
Burke, A. P., Yen, T. S., Shekitka, K. M. & Sobin, L. H. (1990). Lymphoepithelial carcinoma of the stomach with Epstein-Barr virus demonstrated by polymerase chain reaction. Mod Pathol 3, 377-80.
Cannon, J. S., Hamzeh, F., Moore, S., Nicholas, J. & Ambinder, R. F. (1999). Human herpesvirus 8-encoded thymidine kinase and phosphotransferase homologues confer sensitivity to ganciclovir. J Virol 73, 4786-93.
Chee, M. S., Lawrence, G. L. & Barrell, B. G. (1989). Alpha-, beta- and gammaherpesviruses encode a putative phosphotransferase. J Gen Virol 70 ( Pt 5), 1151-60.
Chen, C. & Okayama, H. (1987). High-efficiency transformation of mammalian cells by plasmid DNA. Mol Cell Biol 7, 2745-52.
Chen, M. R., Chang, S. J., Huang, H. & Chen, J. Y. (2000). A protein kinase activity associated with Epstein-Barr virus BGLF4 phosphorylates the viral early antigen EA-D in vitro. J Virol 74, 3093-104.
Chevallier-Greco, A., Manet, E., Chavrier, P., Mosnier, C., Daillie, J. & Sergeant, A. (1986). Both Epstein-Barr virus (EBV)-encoded trans-acting factors, EB1 and EB2, are required to activate transcription from an EBV early promoter. Embo J 5, 3243-9.
Cho, M. S., Milman, G. & Hayward, S. D. (1985). A second Epstein-Barr virus early antigen gene in BamHI fragment M encodes a 48- to 50-kilodalton nuclear protein. J Virol 56, 860-6.
Clough, W. (1979). Deoxyribonuclease activity found in Epstein--Barr virus producing lymphoblastoid cells. Biochemistry 18, 4517-21.
Coulter, L. J., Moss, H. W., Lang, J. & McGeoch, D. J. (1993). A mutant of herpes simplex virus type 1 in which the UL13 protein kinase gene is disrupted. J Gen Virol 74 ( Pt 3), 387-95.
Countryman, J., Jenson, H., Seibl, R., Wolf, H. & Miller, G. (1987). Polymorphic proteins encoded within BZLF1 of defective and standard Epstein-Barr viruses disrupt latency. J Virol 61, 3672-9.
Countryman, J. & Miller, G. (1985). Activation of expression of latent Epstein-Barr herpesvirus after gene transfer with a small cloned subfragment of heterogeneous viral DNA. Proc Natl Acad Sci U S A 82, 4085-9.
Cunningham, C., Davison, A. J., Dolan, A., Frame, M. C., McGeoch, D. J., Meredith, D. M., Moss, H. W. & Orr, A. C. (1992). The UL13 virion protein of herpes simplex virus type 1 is phosphorylated by a novel virus-induced protein kinase. J Gen Virol 73 ( Pt 2), 303-11.
Dambaugh, T., Beisel, C., Hummel, M., King, W., Fennewald, S., Cheung, A., Heller, M., Raab-Traub, N. & Kieff, E. (1980). Epstein-Barr virus (B95-8) DNA VII: molecular cloning and detailed mapping. Proc Natl Acad Sci U S A 77, 2999-3003.
De Bolle, L., Michel, D., Mertens, T., Manichanh, C., Agut, H., De Clercq, E. & Naesens, L. (2002). Role of the human herpesvirus 6 u69-encoded kinase in the phosphorylation of ganciclovir. Mol Pharmacol 62, 714-21.
de Laat, W. L., Jaspers, N. G. & Hoeijmakers, J. H. (1999). Molecular mechanism of nucleotide excision repair. Genes Dev 13, 768-85.
Dolyniuk, M., Pritchett, R. & Kieff, E. (1976). Proteins of Epstein-Barr virus. I. Analysis of the polypeptides of purified enveloped Epstein-Barr virus. J Virol 17, 935-49.
Douville, E., Duncan, P., Abraham, N. & Bell, J. C. (1994). Dual specificity kinases--a new family of signal transducers. Cancer Metastasis Rev 13, 1-7.
Edelman, A. M., Blumenthal, D. K. & Krebs, E. G. (1987). Protein serine/threonine kinases. Annu Rev Biochem 56, 567-613.
Epstein, M. A., Achong, B. G. & Barr, Y. M. (1964). Virus Particles in Cultured Lymphoblasts from Burkitt''s Lymphoma. Lancet 15, 702-3.
Evans, E., Fellows, J., Coffer, A. & Wood, R. D. (1997). Open complex formation around a lesion during nucleotide excision repair provides a structure for cleavage by human XPG protein. Embo J 16, 625-38.
Feighny, R. J., Farrell, M. P. & Pagano, J. S. (1980). Polypeptide synthesis and phosphorylation in Epstein-Barr virus-infected cells. J Virol 34, 455-63.
Fields, S. & Song, O. (1989). A novel genetic system to detect protein-protein interactions. Nature 340, 245-6.
Frame, M. C., Purves, F. C., McGeoch, D. J., Marsden, H. S. & Leader, D. P. (1987). Identification of the herpes simplex virus protein kinase as the product of viral gene US3. J Gen Virol 68 ( Pt 10), 2699-704.
Friedberg, E. C. (1996). Cockayne syndrome--a primary defect in DNA repair, transcription, both or neither? Bioessays 18, 731-8.
Friedberg, E. C. (2001). How nucleotide excision repair protects against cancer. Nat Rev Cancer 1, 22-33.
Friedberg, E. C. (2003). DNA damage and repair. Nature 421, 436-40.
Gershburg, E. & Pagano, J. S. (2002). Phosphorylation of the Epstein-Barr virus (EBV) DNA polymerase processivity factor EA-D by the EBV-encoded protein kinase and effects of the L-riboside benzimidazole 1263W94. J Virol 76, 998-1003.
Grinstein, S., Preciado, M. V., Gattuso, P., Chabay, P. A., Warren, W. H., De Matteo, E. & Gould, V. E. (2002). Demonstration of Epstein-Barr virus in carcinomas of various sites. Cancer Res 62, 4876-8.
Hammerschmidt, W. & Sugden, B. (1988). Identification and characterization of oriLyt, a lytic origin of DNA replication of Epstein-Barr virus. Cell 55, 427-33.
Hanks, S. K. & Hunter, T. (1995). Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification. Faseb J 9, 576-96.
Hardwick, J. M., Lieberman, P. M. & Hayward, S. D. (1988). A new Epstein-Barr virus transactivator, R, induces expression of a cytoplasmic early antigen. J Virol 62, 2274-84.
He, Z., He, Y. S., Kim, Y., Chu, L., Ohmstede, C., Biron, K. K. & Coen, D. M. (1997). The human cytomegalovirus UL97 protein is a protein kinase that autophosphorylates on serines and threonines. J Virol 71, 405-11.
Heineman, T. C. & Cohen, J. I. (1995). The varicella-zoster virus (VZV) open reading frame 47 (ORF47) protein kinase is dispensable for viral replication and is not required for phosphorylation of ORF63 protein, the VZV homolog of herpes simplex virus ICP22. J Virol 69, 7367-70.
Heineman, T. C., Seidel, K. & Cohen, J. I. (1996). The varicella-zoster virus ORF66 protein induces kinase activity and is dispensable for viral replication. J Virol 70, 7312-7.
Henle, G., Henle, W. & Diehl, V. (1968). Relation of Burkitt''s tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc Natl Acad Sci U S A 59, 94-101.
Henle, W., Diehl, V., Kohn, G., Zur Hausen, H. & Henle, G. (1967). Herpes-type virus and chromosome marker in normal leukocytes after growth with irradiated Burkitt cells. Science 157, 1064-5.
Hoeijmakers, J. H. (1994). Human nucleotide excision repair syndromes: molecular clues to unexpected intricacies. Eur J Cancer 30A, 1912-21.
Hoeijmakers, J. H. (2001). Genome maintenance mechanisms for preventing cancer. Nature 411, 366-74.
James, P., Halladay, J. & Craig, E. A. (1996). Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast. Genetics 144, 1425-36.
Jones, J. F., Shurin, S., Abramowsky, C., Tubbs, R. R., Sciotto, C. G., Wahl, R., Sands, J., Gottman, D., Katz, B. Z. & Sklar, J. (1988). T-cell lymphomas containing Epstein-Barr viral DNA in patients with chronic Epstein-Barr virus infections. N Engl J Med 318, 733-41.
Kao, S. Y., Lemoine, F. J. & Marriott, S. J. (2000). Suppression of DNA repair by human T cell leukemia virus type 1 Tax is rescued by a functional p53 signaling pathway. J Biol Chem 275, 35926-31.
Kato, K., Kawaguchi, Y., Tanaka, M., Igarashi, M., Yokoyama, A., Matsuda, G., Kanamori, M., Nakajima, K., Nishimura, Y., Shimojima, M., Phung, H. T., Takahashi, E. & Hirai, K. (2001). Epstein-Barr virus-encoded protein kinase BGLF4 mediates hyperphosphorylation of cellular elongation factor 1delta (EF-1delta): EF-1delta is universally modified by conserved protein kinases of herpesviruses in mammalian cells. J Gen Virol 82, 1457-63.
Kato, K., Yokoyama, A., Tohya, Y., Akashi, H., Nishiyama, Y. & Kawaguchi, Y. (2003). Identification of protein kinases responsible for phosphorylation of Epstein-Barr virus nuclear antigen leader protein at serine-35, which regulates its coactivator function. J Gen Virol 84, 3381-92.
Kawaguchi, Y., Kato, K., Tanaka, M., Kanamori, M., Nishiyama, Y. & Yamanashi, Y. (2003). Conserved protein kinases encoded by herpesviruses and cellular protein kinase cdc2 target the same phosphorylation site in eukaryotic elongation factor 1delta. J Virol 77, 2359-68.
Kawaguchi, Y., Matsumura, T., Roizman, B. & Hirai, K. (1999). Cellular elongation factor 1delta is modified in cells infected with representative alpha-, beta-, or gammaherpesviruses. J Virol 73, 4456-60.
Kawaguchi, Y., Van Sant, C. & Roizman, B. (1998). Eukaryotic elongation factor 1delta is hyperphosphorylated by the protein kinase encoded by the U(L)13 gene of herpes simplex virus 1. J Virol 72, 1731-6.
Kennelly, P. J. & Krebs, E. G. (1991). Consensus sequences as substrate specificity determinants for protein kinases and protein phosphatases. J Biol Chem 266, 15555-8.
Kenyon, T. K., Cohen, J. I. & Grose, C. (2002). Phosphorylation by the varicella-zoster virus ORF47 protein serine kinase determines whether endocytosed viral gE traffics to the trans-Golgi network or recycles to the cell membrane. J Virol 76, 10980-93.
Kenyon, T. K., Lynch, J., Hay, J., Ruyechan, W. & Grose, C. (2001). Varicella-zoster virus ORF47 protein serine kinase: characterization of a cloned, biologically active phosphotransferase and two viral substrates, ORF62 and ORF63. J Virol 75, 8854-8.
Klein, G., Clifford, P., Klein, E. & Stjernsward, J. (1966). Search for tumor-specific immune reactions in Burkitt lymphoma patients by the membrane immunofluorescence reaction. Proc Natl Acad Sci U S A 55, 1628-35.
Krosky, P. M., Baek, M. C. & Coen, D. M. (2003). The human cytomegalovirus UL97 protein kinase, an antiviral drug target, is required at the stage of nuclear egress. J Virol 77, 905-14.
Kunz, B. A., Straffon, A. F. & Vonarx, E. J. (2000). DNA damage-induced mutation: tolerance via translesion synthesis. Mutat Res 451, 169-85.
Leader, D. P. (1993). Viral protein kinases and protein phosphatases. Pharmacol Ther 59, 343-89.
Littler, E., Stuart, A. D. & Chee, M. S. (1992). Human cytomegalovirus UL97 open reading frame encodes a protein that phosphorylates the antiviral nucleoside analogue ganciclovir. Nature 358, 160-2.
Liu, M. T., Chen, Y. R., Chen, S. C., Hu, C. Y., Lin, C. S., Chang, Y. T., Wang, W. B. & Chen, J. Y. (2004). Epstein-Barr virus latent membrane protein 1 induces micronucleus formation, represses DNA repair and enhances sensitivity to DNA-damaging agents in human epithelial cells. Oncogene 23, 2531-9.
Long, M. C., Leong, V., Schaffer, P. A., Spencer, C. A. & Rice, S. A. (1999). ICP22 and the UL13 protein kinase are both required for herpes simplex virus-induced modification of the large subunit of RNA polymerase II. J Virol 73, 5593-604.
Luka, J., Kallin, B. & Klein, G. (1979). Induction of the Epstein-Barr virus (EBV) cycle in latently infected cells by n-butyrate. Virology 94, 228-31.
Marschall, M., Freitag, M., Suchy, P., Romaker, D., Kupfer, R., Hanke, M. & Stamminger, T. (2003). The protein kinase pUL97 of human cytomegalovirus interacts with and phosphorylates the DNA polymerase processivity factor pUL44. Virology 311, 60-71.
Marschall, M., Stein-Gerlach, M., Freitag, M., Kupfer, R., van den Bogaard, M. & Stamminger, T. (2002). Direct targeting of human cytomegalovirus protein kinase pUL97 by kinase inhibitors is a novel principle for antiviral therapy. J Gen Virol 83, 1013-23.
Matsunaga, T., Mu, D., Park, C. H., Reardon, J. T. & Sancar, A. (1995). Human DNA repair excision nuclease. Analysis of the roles of the subunits involved in dual incisions by using anti-XPG and anti-ERCC1 antibodies. J Biol Chem 270, 20862-9.
Miller, R. L., Glaser, R. & Rapp, F. (1977). Studies of an Epstein-Barr virus-induced DNA polymerase. Virology 76, 494-502.
Moffat, J. F., Zerboni, L., Sommer, M. H., Heineman, T. C., Cohen, J. I., Kaneshima, H. & Arvin, A. M. (1998). The ORF47 and ORF66 putative protein kinases of varicella-zoster virus determine tropism for human T cells and skin in the SCID-hu mouse. Proc Natl Acad Sci U S A 95, 11969-74.
Morrison, E. E., Wang, Y. F. & Meredith, D. M. (1998). Phosphorylation of structural components promotes dissociation of the herpes simplex virus type 1 tegument. J Virol 72, 7108-14.
Ng, T. I. & Grose, C. (1992). Serine protein kinase associated with varicella-zoster virus ORF 47. Virology 191, 9-18.
Ng, T. I., Keenan, L., Kinchington, P. R. & Grose, C. (1994). Phosphorylation of varicella-zoster virus open reading frame (ORF) 62 regulatory product by viral ORF 47-associated protein kinase. J Virol 68, 1350-9.
Ng, T. I., Ogle, W. O. & Roizman, B. (1998). UL13 protein kinase of herpes simplex virus 1 complexes with glycoprotein E and mediates the phosphorylation of the viral Fc receptor: glycoproteins E and I. Virology 241, 37-48.
Niedobitek, G., Young, L. S., Lau, R., Brooks, L., Greenspan, D., Greenspan, J. S. & Rickinson, A. B. (1991). Epstein-Barr virus infection in oral hairy leukoplakia: virus replication in the absence of a detectable latent phase. J Gen Virol 72 ( Pt 12), 3035-46.
Ogle, W. O., Ng, T. I., Carter, K. L. & Roizman, B. (1997). The UL13 protein kinase and the infected cell type are determinants of posttranslational modification of ICP0. Virology 235, 406-13.
Overton, H. A., McMillan, D. J., Klavinskis, L. S., Hope, L., Ritchie, A. J. & Wong-kai-in, P. (1992). Herpes simplex virus type 1 gene UL13 encodes a phosphoprotein that is a component of the virion. Virology 190, 184-92.
Park, J., Lee, D., Seo, T., Chung, J. & Choe, J. (2000). Kaposi''s sarcoma-associated herpesvirus (human herpesvirus-8) open reading frame 36 protein is a serine protein kinase. J Gen Virol 81, 1067-71.
Prichard, M. N., Gao, N., Jairath, S., Mulamba, G., Krosky, P., Coen, D. M., Parker, B. O. & Pari, G. S. (1999). A recombinant human cytomegalovirus with a large deletion in UL97 has a severe replication deficiency. J Virol 73, 5663-70.
Purves, F. C., Ogle, W. O. & Roizman, B. (1993). Processing of the herpes simplex virus regulatory protein alpha 22 mediated by the UL13 protein kinase determines the accumulation of a subset of alpha and gamma mRNAs and proteins in infected cells. Proc Natl Acad Sci U S A 90, 6701-5.
Purves, F. C. & Roizman, B. (1992). The UL13 gene of herpes simplex virus 1 encodes the functions for posttranslational processing associated with phosphorylation of the regulatory protein alpha 22. Proc Natl Acad Sci U S A 89, 7310-4.
Quinlan, M. P., Chen, L. B. & Knipe, D. M. (1984). The intranuclear location of a herpes simplex virus DNA-binding protein is determined by the status of viral DNA replication. Cell 36, 857-68.
Reddy, S. M., Cox, E., Iofin, I., Soong, W. & Cohen, J. I. (1998). Varicella-zoster virus (VZV) ORF32 encodes a phosphoprotein that is posttranslationally modified by the VZV ORF47 protein kinase. J Virol 72, 8083-8.
Sancar, A., Lindsey-Boltz, L. A., Unsal-Kaccmaz, K. & Linn, S. (2004). Molecular Mechanisms of Mammalian DNA Repair and the DNA Damage Checkpoints. Annu Rev Biochem 73, 39-85.
Santagati, F., Botta, E., Stefanini, M. & Pedrini, A. M. (2001). Different dynamics in nuclear entry of subunits of the repair/transcription factor TFIIH. Nucleic Acids Res 29, 1574-81.
Schang, L. M., Rosenberg, A. & Schaffer, P. A. (1999). Transcription of herpes simplex virus immediate-early and early genes is inhibited by roscovitine, an inhibitor specific for cellular cyclin-dependent kinases. J Virol 73, 2161-72.
Schepers, A., Pich, D. & Hammerschmidt, W. (1996). Activation of oriLyt, the lytic origin of DNA replication of Epstein-Barr virus, by BZLF1. Virology 220, 367-76.
Skare, J. & Strominger, J. L. (1980). Cloning and mapping of BamHi endonuclease fragments of DNA from the transforming B95-8 strain of Epstein-Barr virus. Proc Natl Acad Sci U S A 77, 3860-4.
Smith, R. F. & Smith, T. F. (1989). Identification of new protein kinase-related genes in three herpesviruses, herpes simplex virus, varicella-zoster virus, and Epstein-Barr virus. J Virol 63, 450-5.
Soong, W., Schultz, J. C., Patera, A. C., Sommer, M. H. & Cohen, J. I. (2000). Infection of human T lymphocytes with varicella-zoster virus: an analysis with viral mutants and clinical isolates. J Virol 74, 1864-70.
Stevenson, D., Colman, K. L. & Davison, A. J. (1994). Characterization of the putative protein kinases specified by varicella-zoster virus genes 47 and 66. J Gen Virol 75 ( Pt 2), 317-26.
Sugasawa, K., Ng, J. M., Masutani, C., Iwai, S., van der Spek, P. J., Eker, A. P., Hanaoka, F., Bootsma, D. & Hoeijmakers, J. H. (1998). Xeroderma pigmentosum group C protein complex is the initiator of global genome nucleotide excision repair. Mol Cell 2, 223-32.
Sugasawa, K., Okamoto, T., Shimizu, Y., Masutani, C., Iwai, S. & Hanaoka, F. (2001). A multistep damage recognition mechanism for global genomic nucleotide excision repair. Genes Dev 15, 507-21.
Sugawara, Y., Makuuchi, M. & Takada, K. (2000). Detection of Epstein-Barr virus DNA in hepatocellular carcinoma tissues from hepatitis C-positive patients. Scand J Gastroenterol 35, 981-4.
Svetlova, M., Nikiforov, A., Solovjeva, L., Pleskach, N., Tomilin, N. & Hanawalt, P. C. (1999). Reduced extractability of the XPA DNA repair protein in ultraviolet light-irradiated mammalian cells. FEBS Lett 463, 49-52.
Tang, J. & Chu, G. (2002). Xeroderma pigmentosum complementation group E and UV-damaged DNA-binding protein. DNA Repair (Amst) 1, 601-16.
Taylor, T. J. & Knipe, D. M. (2004). Proteomics of herpes simplex virus replication compartments: association of cellular DNA replication, repair, recombination, and chromatin remodeling proteins with ICP8. J Virol 78, 5856-66.
Tovey, M. G., Lenoir, G. & Begon-Lours, J. (1978). Activation of latent Epstein-Barr virus by antibody to human IgM. Nature 276, 270-2.
Tsurumi, T. (1993). Purification and characterization of the DNA-binding activity of the Epstein-Barr virus DNA polymerase accessory protein BMRF1 gene products, as expressed in insect cells by using the baculovirus system. J Virol 67, 1681-7.
Tsurumi, T., Kishore, J., Yokoyama, N., Fujita, M., Daikoku, T., Yamada, H., Yamashita, Y. & Nishiyama, Y. (1998). Overexpression, purification and helix-destabilizing properties of Epstein-Barr virus ssDNA-binding protein. J Gen Virol 79 ( Pt 5), 1257-64.
Uchida, A., Sugasawa, K., Masutani, C., Dohmae, N., Araki, M., Yokoi, M., Ohkuma, Y. & Hanaoka, F. (2002). The carboxy-terminal domain of the XPC protein plays a crucial role in nucleotide excision repair through interactions with transcription factor IIH. DNA Repair (Amst) 1, 449-61.
van Zeijl, M., Fairhurst, J., Baum, E. Z., Sun, L. & Jones, T. R. (1997). The human cytomegalovirus UL97 protein is phosphorylated and a component of virions. Virology 231, 72-80.
Weiss, L. M., Strickler, J. G., Warnke, R. A., Purtilo, D. T. & Sklar, J. (1987). Epstein-Barr viral DNA in tissues of Hodgkin''s disease. Am J Pathol 129, 86-91.
Wilcock, D. & Lane, D. P. (1991). Localization of p53, retinoblastoma and host replication proteins at sites of viral replication in herpes-infected cells. Nature 349, 429-31.
Wilkinson, D. E. & Weller, S. K. (2004). Recruitment of cellular recombination and repair proteins to sites of herpes simplex virus type 1 DNA replication is dependent on the composition of viral proteins within prereplicative sites and correlates with the induction of the DNA damage response. J Virol 78, 4783-96.
Wolf, D. G., Courcelle, C. T., Prichard, M. N. & Mocarski, E. S. (2001). Distinct and separate roles for herpesvirus-conserved UL97 kinase in cytomegalovirus DNA synthesis and encapsidation. Proc Natl Acad Sci U S A 98, 1895-900.
Yokoyama, N., Fujii, K., Hirata, M., Tamai, K., Kiyono, T., Kuzushima, K., Nishiyama, Y., Fujita, M. & Tsurumi, T. (1999). Assembly of the epstein-barr virus BBLF4, BSLF1 and BBLF2/3 proteins and their interactive properties. J Gen Virol 80 ( Pt 11), 2879-87.
Zheng, L., Pan, H., Li, S., Flesken-Nikitin, A., Chen, P. L., Boyer, T. G. & Lee, W. H. (2000). Sequence-specific transcriptional corepressor function for BRCA1 through a novel zinc finger protein, ZBRK1. Mol Cell 6, 757-68.
Zhou, B. B. & Elledge, S. J. (2000). The DNA damage response: putting checkpoints in perspective. Nature 408, 433-9.
胡祺苑 (2000) EB病毒之潛伏蛋白1、核抗原1及DNA分解酶對基因體不穩定性的影響 碩士學位論文
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