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研究生:凌啟鴻
研究生(外文):Chi-Hung Ling
論文名稱:白靈側耳分子生物鑑定系統之研究
論文名稱(外文):Molecular systematics of Pleurotus nebrodensis
指導教授:許瑞祥許瑞祥引用關係
指導教授(外文):Ruey-Shyang Hseu
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:微生物與生化學研究所
學門:生命科學學門
學類:微生物學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:91
中文關鍵詞:白靈側耳側耳屬食用真菌核糖體RNA分子鑑定
外文關鍵詞:ribosomal RNAmolecular systematicsmushroomPleurotus nebrodensis
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白靈側耳(Pleurotus nebrodensis)為食用菇菌的一種,子實體色澤潔白,個體碩大,兼具有營養、嗜好等特性,極具商業開發潛力。然而白靈側耳子實體形態受環境因子及遺傳變異影響,且經過馴化改良後的生產用菌株形態差異更大,若僅以子實體形態及菌株生理生化特性作為分類依據將造成混淆。本論文以傳統形態觀察及分子生物技術進行白靈側耳的分類及育種研究。並由擔孢子篩選單核體進行全機率組合配對,確認白靈側耳之交配模式為雙因子控制的四極型。依照此交配模式進行雜交,共獲得30株菌落形態、生長速率與親代比較各有不同的雜交株。而以核醣體RNA基因探討親緣關係時,顯示ITS (internal transcribed spacer)區域序列可區分側耳屬(Pleurotus)中物種。根據由ITS1區域序列演算所建立的親緣關係圖與其限制酶水解多型性圖譜RFLP (Restriction Fragment Length Polymorphism)可區別白靈側耳與刺芹側耳(Pleurotus eryngii);利用設計的引子組EF-f / EF-r建立具種專一性鑑別能力的聚合酶連鎖反應(Species-Specific PCR)可明確辨認白靈側耳Pleurotus nebrodensis,且此鑑別系統所需最低的白靈側耳DNA濃度為2.5 pg/μl。
Pleurotus nebrodensis is edible mushroom which has rich nutrition, good flavor and taste. Its pure white fruitbody with high biomass makes it potentially commercialized. However, the morphology of P. nebrodensis fruitbodies is frequently influence by environmental factors and hereditary variation that commercial strains would have obvious variety in morphology after long-term artificial cultivations. The classification basis would be confused if the researchers just depended on the morphology of fruitbody, biochemical characteristic and physiology. In order to realize the relationships of P. nebrodensis species, this study aim at the classification and identification of P. nebrodensis by using molecular techniques. The characteristics of P. nebrodensis were investigated by observing fruitbodies, basidia, spores, and hyphae structure. There were 48 monokaryon isolated from basidiospores and 12 selected strains mated randomly. The mating type of P. nebrodensis was confirmed as tetra-polar type. According to this result, opposite sex of P. nebrodensis strains were hybridized that 30 dikaryotic strains had differences in mycelium morphology and growth rate with P. nebrodensis strain WW. The phylogenic relationship of P. nebrodensis was elucidated by using ribosomal RNA gene sequences that the Pleurotus species could be distinguished by ITS1 (internal transcribed spacer) sequences. Based on phylogenetic relationship, RFLP (restriction fragment length polymorphism) could distinguish between P. nebrodensis and P. eryngii. Furthermore, a species-specific polymerase chain reaction (PCR) technique was developed by using primer set EF-f and EF-r designed from specific sequence of ITS1 region in P. nebrodensis. The minimal concentration of DNA required for PCR was 2.5 pg/μl.
中文摘要 4
Abstract 5
表目錄 6
圖目錄 7
圖目錄 7
第一章 前言 9
第一章 前言 9
食用菇菌概述 9
二、側耳屬真菌 15
三、白靈側耳(Pleurotus nebrodensis) 17
四、側耳屬分類研究現況與鑑定系統 19
五、研究動機與目的 21
第二章 材料與方法 23
一、實驗材料 23
1.實驗標本及菌株 23
2.實驗藥品 25
3.實驗儀器 26
二、實驗方法 27
1.菌株生理及遺傳特性 27
2.核糖體RNA基因序列夾取 32
3.限制酶水解多型性圖譜鑑別法 36
4.白靈側耳專一性鑑別引子設計 37
第三章 實驗結果 39
一、白靈側耳型態及生理觀察 39
二、單-雙核親和配對測試 40
三、交配因子決定法 40
四、白靈側耳雜交株生理觀察 41
五、核糖體RNA基因序列夾取 41
六、限制酶水解多型性圖譜鑑別法 43
七、白靈側耳專一性鑑別引子設計 43
第四章 討論 45
第五章 結論 50
參考文獻 88
1.Atallings, W. C., K. A. Pattridge, R. K. Strong, and M. L. Ludwig. 1985. The structure of manganese superoxide dismutase from Thermus thermophilus HB8 at 2.4-A resolution. The Journal of Biological Chemistry 260:16424-16432.

2.Bano, Z. A. S. R. 1986. Vitamin values of Pleurotus mushrooms. Qualitas Plantarum Plant Foods for Human Nutrition 36:11-16.

3.Butler, G. M. 1968. Environmentally induced changes in clamp connection incidance in hyphal branching systems of Coprinus disseminatus. Annales of Botany (London) 32:847.

4.Chang, S. T. 1999. Global impact of edible and medicinal mushrooms on human welfare in the 21st Century: Nongreen revolution. International Journal of Medicinal Mushrooms 1:1-8.

5.Chang, S. T. 1999. World production of cultivated edible and medicinal mushrooms in 1997 with emphasis on Lentinula endodes (Berk.) Sing. in China. International Journal of Medicinal Mushrooms 1:310-318.

6.Eger, G. 1978. Biology and breeding of Pleurotus. In Biology and cultivation of edible mushrooms. New York, San Francisco, London: Academic Press:497-519.

7.Eger, G., G. Eden and E. Wissig. 1976. Pleurotus ostreatus - breeding potential of a new cultivated mushroom. Theoretical and Applied Genetics 47:155-163.

8.Gonzalez, P., and J. Labarere. 2000. Phylogenetic relationships of Pleurotus species according to the sequence and secondary structure of the mitochondrial small-subunit rRNA V4, V6 and V9 domains. Microbiology 146 ( Pt 1):209-21.

9.Hilber, O. 1982. Die Gattung Pleurotus (Fr.) Kummer. Biblitheca Mycologia 87:464.

10.Hilber, O. 1989. Valid, invalid and confusing taxa of the genus Pleurotus. Mushroom Science 12:241-248.

11.Hilber, O. 1997. The Genus Pleurotus (Fr.) Kummer (2). Ershienen im Selbstverlag:64 pp.

12.Hughes, P. R. H. a. K. W. 1997. A New species of Pleurotus. Mycologia 89:173-180.

13.Kaarik, A. 1992. Pleurotus (Fr.) Quel., Macromycetes Vol. 2:pp. 48-49.

14.Kavanagh, F., D. Hervey and W. J. Robbins. 1951. Antibiotic substances from Basidiomycetes. VIII, Pleurotus mutilis (Fr.) Sacc. and Pleurotus passeckerianus Pilat. Proceeding National Acagemic Science. U.S.A. 37:570.

15.Kazuno, C. A. H. M. 1985. Chemical Constituents of Pleurotus: studies on constituents of edible fungi. Journal of Japanese Society for Food Science and Technology 32:338-343.

16.Khanna, P. A. G., H. S. 1984. Pleurotus mushroom - a source of food protein. Mushroom newsletter for the topics 4:9-15.

17.Lewinsohn D., W. S. P., Reshetnikov S. V., Hadar Y., Nevo E. 2002. The Pleurotus eryngii species-complex in Isreal:distribution and morphological description of a new taxon. Mycotaxon LXXXI:51-67.

18.Moser, M. 1983. Die Rohrlinge und Blatterpilze (Polyporales, Boletales, Agricales, Russulales). 5 vol. ub. Aufl.-stuttgart; New York: Fischer, Bd.2, (Kleine Kryptogamenflora. Begrundet von H. Dams).

19.Singer, R. 1986. The Agaricales in moderm taxonomy. Koeltz Sci, Books, Koenigstein:981.

20.Sun, R. V. A. B. L. 1994. Ancient and recent patterns of phylogentic analysis in osyter mushroom Pleurotus revealed by phylogenetic analysis of rinodome DNA sequence. 91:4599 - 4603.

21.Venturella, G. 2002. On the real identity of Plrutotus nebrodensis in Spain. Mycotaxon LXXXIV:445-446.

22.Venturella, G. 2000. Typification of Pleurotus nebrodensis. Mycotaxon LXXV:229-231.

23.Watling, R. A. N. M. G. 1989. 6/Crepidotaceae, Pleurotaceae and other pleurotoid agarics. In: British Fungus Flora, Agarics and Boleti. Royal Botanic Gardens. Edinburgh:157 pp.

24.Yoshioka, Y., T. Ikekana, M. Noda and F. Fukuoka. 1972. Studies on antitumor activity of some fractions from Basidiomycetes, I. An antitumer acidic polysaccharide fraction of Pleurotus ostreatus (Fr.) Quel. Chemical & pharmaceutical bulletin 20:1175-1180.

25.Zadrazil, F. 1978. Cultivation of Pleurotus. In the biology and cultivation of edible mushrooms. New York, San Francisco, London: Academic Press.

26.Zervakis, G. A. C. B. 1996. A pluralistic approach in the study of Pleurotus species with emphasis on compatibility and physiology of the European morphotaxa. Micological Research 100:717-731.

27.Zervakis, G. A. G. V. 1998. Toward the elucidation of systematics of the Pleurotus taxa growing in Umbellifers. In Abstracts of the Sixth Internationalal Mycological Congress, Jerusalem, Israel:7.

28.Zervakis, G. I., G. Venturella, and K. Papadopoulou. 2001. Genetic polymorphism and taxonomic infrastructure of the Pleurotus eryngii species-complex as determined by RAPD analysis, isozyme profiles and ecomorphological characters. Microbiology 147:3183-94.

29.水野 卓, 川合正允著., 賴慶亮譯. 1997. 菇類的化學.生化學. 國立編譯館.

30.杜自彊. 1974. 食用菇栽培技術. 豐年社.

31.許文輝. 1980. 鮑魚菇屬人工栽培彩色圖鑑. 食品工業月刊社.

32.游中驥. 1993. 食用菇栽培. 淑馨出版社.
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