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研究生:吳岱融
研究生(外文):Dai-Rong Wu
論文名稱:以SSR分子標記測量水稻品種內之遺傳均一性
論文名稱(外文):Genetic Uniformity within Rice Cultivars Measured by SSR Markers
指導教授:胡凱康
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:農藝學研究所
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:43
中文關鍵詞:分子標記均一性品種內
外文關鍵詞:within cultivaruniformitySSR
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本實驗使用20對SSR分子標記及螢光標記自動偵測技術,對六個水稻品種進行品種內變異性調查。所使用的SSR分子標記共可偵測到21個基因座,其中有18個基因座在品種間表現多型性,有11個基因座在品種內表現多型性。品種內變異性來源主要有兩種,為同一基因座內不同類型的同型合子,以及異型合子,其中前者占絕大部分。藉由主成分分析法、分群分析法及Assignment test分析實驗結果顯示,水稻品種內依然存在不同程度的變異性,但其不足以影響品種間的區分。在品種間與品種內變異性界線的討論上,本實驗引用了遺傳距離與Assignment test兩種方式,對品種間與品種內變異性進行觀察,結果顯示絕大部分樣品點的基因資訊符合界線的規範。水稻品種的變異性來源可能為突變、殘留的異質性、樣品的種子純度、異交率與實驗誤差,其中實驗誤差包含了品種數目與品種內取樣數目等。
The within-cultivar uniformity of six rice cultivar has been investigated using 20 SSR markers with fluorescence-based automated DNA detection technology in this study. In all 22 loci detected, 18 and 11 loci are polymorphic between and within cultivar, respectively. There are two type of non-uniformity within cultivars, the overwhelming one is the type that has more than one homozygotes in a locus, the other one is hetetozygote. According to the result of principle coordinate analysis, cluster analysis and Assignment test, the cultivars were uniquely identifiable in spite of different level of existing within-cultivar non-uniformity. To discuss the boundary of distinctness between cultivars and uniformity within cultivars, genetic distance and Assignment test were used, and the result revealed genetic information of most samples fall within the standards. The within-cultivar non-uniformity may come from mutation, residual heterozygosity, the seed purity of rice sample used, open-pollination rate and experimental error, which includes the number of cultivars used and number of plants within cultivars sampled.
內容目錄 i
圖片目錄 ii
表格目錄 iii
摘要 iv
Abstract v
前言 1
前人研究 2
材料與方法 7
材料準備 7
DNA萃取 7
DNA品質檢定 9
DNA稀釋定量 9
DNA片段的擴增 9
引子 (primer) 9
聚合酵素連鎖反應 (Polymerase Chain Reaction, PCR) 10
擴增結果資料收集 11
分子資料分析 11
結果 14
討論 29
品種間與品種內的變異形式 29
品種間與品種內的變異 29
品種間與品種內變異性的界線 30
以相似性定界線 30
以機率試算定界線 31
品種內變異性來源 32
突變 32
殘留的異質性 33
兩種對偶基因頻度差異不大的基因座 35
種子混雜與異交率 35
取樣族群大小 37
分子標記結果與田間表現 38
參考文獻 40
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