本研究主要在於利用液態基質進行鴻喜菇菌絲體的培養，探討不同培養條件對液態培養鴻喜菇菌絲體生長與生理活性成分生成之影響。
　　利用二種不同的培養基，MY及PD，在不同pH值的條件下，進行鴻喜菇的液態菌種培養。隨著時間的增加，菌絲體生成量上升，pH值也跟著升高，以pH4.5培養基為PD是鴻喜菇液態菌種製備的最適條件。經醱酵槽培養後，接種30mL至木屑瓶中，到子實體採收所需時間比傳統的固態菌種栽培大幅減少了44.1 %。
子實體多醣含量測試結果為乾燥菇傘(20.2 mg/g)＞乾燥完整子實體(20 mg/g)＞乾燥菇柄(19.3mg/g)。利用搖瓶培養，醱酵液中多醣含量隨著培養天數的增加而減少，但醱酵槽培養時，卻是在減少數日後，多醣含量有再上升的現象。
　　另外，在抗氧化能力分析中，乾燥子實體的甲醇萃取物DPPH清除率＞菌絲體的甲醇萃取物；乾燥子實體的甲醇萃取物還原力＞菌絲體甲醇萃取物；乾燥子實體的甲醇萃取物螯合亞鐵離子的能力＞菌絲體甲醇萃取物；子實體的熱水萃取物清除超氧陰離子能力＞菌絲體的熱水萃取物。
而在腫瘤細胞測試方面，在濃度0.25~4.0 mg/mL的範圍裡，乾燥子實體的甲醇萃取物對於人類肺癌細胞(A549 cells)作用72小時，能使腫瘤細胞存活率減低。至於人類乳癌細胞(MCF7 cells) 乾燥子實體的甲醇及乙醇萃取物和熱水萃取的乾燥子實體都有不錯的抑制表現；另外，乾燥子實體的甲醇萃取物可以抑制人類肝癌細胞(Hep3B cells)的生長，液態培養菌絲體萃取物及醱酵液均無抑制腫瘤細胞之表現。

中文摘要…………………………………………………………...…………………i
英文摘要…………………………………………………………...………………ii
致謝………………………………………….………………………………………iii
目次………………………………………….………………………………………iv
第一章 文獻整理…………………………………………………………………..1
1.1菇類的生理活性功能………………………………………………………….1
1.1.1菇類簡介…………………………………………………………………..1
1.1.2菇類的生理活性功能……………………………………………………..4
1.2鴻喜菇簡介與相關研究……………………………………………………….5
1.3液態培養……………………………………………………………………….7
1.4自由基與健康的關係………………………………………………………….9
1.4.1 自由基與活性氧………………………………………………………10
1.4.2活性氧與自由基之形成…………………………………………………10
1.4.3活性氧、自由基和氧化產物對生物體分子之傷害……………………12
1.4.4天然抗氧化物的種類及作用機制……………………………………...14
1.4.5常見的抗氧化能力測定方法………………………………………...…19
第二章 研究目的 ……………………………………………………………….21
第三章 材料與方法
3.1實驗材料……………………………………………………………………...22
3.1.1設備………………………………………………………………………22
3.1.2藥品………………………………………………………………………22
3.1.3鴻喜菇……………………………………………………………………24
3.1.4細胞株……………………………………………………………………24
3.2實驗方法……………………………………………………………………...24
3.2.1液態醱酵培養菌絲體……………………………………………………24
3.2.2子實體萃取物製備………………………………………………………25
3.2.3液態培養萃取物製備…..………………………………………………..26
3.3多醣含量測定……………..……………………………………………….27
3.3.1多醣樣品製備……………………………………………………………27
3.3.2多醣含量測定……………………………………………………………27
3.4抗氧化性質分析……………………………………………………………...28
3.4.1樣品的製備………………………………………………………………28
3.4.2捕捉DPPH能力之測定…………………………………………………28
3.4.3還原力之測定……………………………………………………………28
3.4.4螯合亞鐵離子能力之測定………………………………………………29
3.4.5清除超氧陰離子能力之測定…………………………………………...29
3.5細胞株測試…………………………………………………………………...29
第四章 結果與討論………………………………………………………………33
4.1鴻喜菇液態培養生長情形…………………………………………………..33
4.1.1搖瓶培養……………………………..…………….…………………….33
4.1.2 5L醱酵槽培養…………………………………………………………47
4.1.3液態菌種接種後出菇情形…………………………………………….54
4.2多醣含量測定……….………………………………………………………..55
4.2.1搖瓶培養多醣含量測定…………………………………………………56
4.2.2 5L醱酵槽醱酵液多醣含量測定………………………………………..56
4.2.3 乾燥子實體多醣含量測定……………………………………………..59
4.3抗氧化性質分析……………………………………………………………..59
4.3.1捕捉DPPH能力之測定……………………………………………........59
4.3.2還原力之測定……………………………………………………………67
4.3.3螯合亞鐵離子能力之測定……………………………………………...71
4.3.4清除超氧陰離子能力之測定………………………………………….74
4.4腫瘤細胞測試………………………………………………………………..74
4.4.1鴻喜菇子實體及菌絲體之甲醇萃取物對抑制人類肺癌細胞(A549 cells)之影響…………………………………………………………………76
4.4.2鴻喜菇子實體及菌絲體之乙醇萃取物對抑制人類肺癌細胞(A549 cells)之影響………………………………………………………………........76
4.4.3鴻喜菇子實體及菌絲體之熱水萃取物對抑制人類肺癌細胞(A549 cells)之影響……………………………………………………………………80
4.4.4鴻喜菇醱酵液對抑制人類肺癌細胞(A549 cells)之影響………….…..80
4.4.5鴻喜菇子實體及菌絲體之甲醇萃取物對抑制人類乳癌細胞(MCF7 cells)之影響…………………..…………………………………..…….84
4.4.6鴻喜菇子實體及菌絲體之乙醇萃取物對抑制人類乳癌細胞(MCF7 cells)之影響………………………………………………………………84
4.4.7鴻喜菇子實體及菌絲體之熱水萃取物對抑制人類乳癌細胞(MCF7 cells)之影響………………………………………………………………88
4.4.8鴻喜菇醱酵液對抑制人類乳癌細胞(MCF7 cells)之影響…………..88
4.4.9鴻喜菇子實體及菌絲體之甲醇萃取物對抑制人類肝癌細胞(Hep3B cells)之影響…………………………………..……………………….…92
4.4.10鴻喜菇子體及菌絲體之乙醇萃取物對抑制人類肝癌細胞(Hep3B cells)之影響……………………………………………………………………96
4.4.11鴻喜菇子實體及菌絲體之熱水萃取物對抑制人類肝癌細胞(Hep3B cells)之影響……………………………………………………….……..96
4.4.12鴻喜菇醱酵液對抑制人類肝癌細胞(Hep3B cells)之影響…………...96
第五章 結論…………………………………………………………….……….101
參考文獻…………………………………………………………………………104
附錄…

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