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研究生:鄭靖緹
論文名稱:鴻喜菇之液態菌種培養及功能性評估
論文名稱(外文):The Study of Cultivation Parameters of Hypsiagus Liquid Spawn and Functional Assessment
指導教授:賴龍標李順來李順來引用關係許孟博
學位類別:碩士
校院名稱:南台科技大學
系所名稱:化學工程系
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
中文關鍵詞:鴻喜菇液態培養抗氧化能力多醣癌細胞株
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  本研究主要在於利用液態基質進行鴻喜菇菌絲體的培養,探討不同培養條件對液態培養鴻喜菇菌絲體生長與生理活性成分生成之影響。
  利用二種不同的培養基,MY及PD,在不同pH值的條件下,進行鴻喜菇的液態菌種培養。隨著時間的增加,菌絲體生成量上升,pH值也跟著升高,以pH4.5培養基為PD是鴻喜菇液態菌種製備的最適條件。經醱酵槽培養後,接種30mL至木屑瓶中,到子實體採收所需時間比傳統的固態菌種栽培大幅減少了44.1 %。
子實體多醣含量測試結果為乾燥菇傘(20.2 mg/g)>乾燥完整子實體(20 mg/g)>乾燥菇柄(19.3mg/g)。利用搖瓶培養,醱酵液中多醣含量隨著培養天數的增加而減少,但醱酵槽培養時,卻是在減少數日後,多醣含量有再上升的現象。
  另外,在抗氧化能力分析中,乾燥子實體的甲醇萃取物DPPH清除率>菌絲體的甲醇萃取物;乾燥子實體的甲醇萃取物還原力>菌絲體甲醇萃取物;乾燥子實體的甲醇萃取物螯合亞鐵離子的能力>菌絲體甲醇萃取物;子實體的熱水萃取物清除超氧陰離子能力>菌絲體的熱水萃取物。
而在腫瘤細胞測試方面,在濃度0.25~4.0 mg/mL的範圍裡,乾燥子實體的甲醇萃取物對於人類肺癌細胞(A549 cells)作用72小時,能使腫瘤細胞存活率減低。至於人類乳癌細胞(MCF7 cells) 乾燥子實體的甲醇及乙醇萃取物和熱水萃取的乾燥子實體都有不錯的抑制表現;另外,乾燥子實體的甲醇萃取物可以抑制人類肝癌細胞(Hep3B cells)的生長,液態培養菌絲體萃取物及醱酵液均無抑制腫瘤細胞之表現。
中文摘要…………………………………………………………...…………………i
英文摘要…………………………………………………………...………………ii
致謝………………………………………….………………………………………iii
目次………………………………………….………………………………………iv
第一章 文獻整理…………………………………………………………………..1
1.1菇類的生理活性功能………………………………………………………….1
1.1.1菇類簡介…………………………………………………………………..1
1.1.2菇類的生理活性功能……………………………………………………..4
1.2鴻喜菇簡介與相關研究……………………………………………………….5
1.3液態培養……………………………………………………………………….7
1.4自由基與健康的關係………………………………………………………….9
1.4.1 自由基與活性氧………………………………………………………10
1.4.2活性氧與自由基之形成…………………………………………………10
1.4.3活性氧、自由基和氧化產物對生物體分子之傷害……………………12
1.4.4天然抗氧化物的種類及作用機制……………………………………...14
1.4.5常見的抗氧化能力測定方法………………………………………...…19
第二章 研究目的 ……………………………………………………………….21
第三章 材料與方法
3.1實驗材料……………………………………………………………………...22
3.1.1設備………………………………………………………………………22
3.1.2藥品………………………………………………………………………22
3.1.3鴻喜菇……………………………………………………………………24
3.1.4細胞株……………………………………………………………………24
3.2實驗方法……………………………………………………………………...24
3.2.1液態醱酵培養菌絲體……………………………………………………24
3.2.2子實體萃取物製備………………………………………………………25
3.2.3液態培養萃取物製備…..………………………………………………..26
3.3多醣含量測定……………..……………………………………………….27
3.3.1多醣樣品製備……………………………………………………………27
3.3.2多醣含量測定……………………………………………………………27
3.4抗氧化性質分析……………………………………………………………...28
3.4.1樣品的製備………………………………………………………………28
3.4.2捕捉DPPH能力之測定…………………………………………………28
3.4.3還原力之測定……………………………………………………………28
3.4.4螯合亞鐵離子能力之測定………………………………………………29
3.4.5清除超氧陰離子能力之測定…………………………………………...29
3.5細胞株測試…………………………………………………………………...29
第四章 結果與討論………………………………………………………………33
4.1鴻喜菇液態培養生長情形…………………………………………………..33
4.1.1搖瓶培養……………………………..…………….…………………….33
4.1.2 5L醱酵槽培養…………………………………………………………47
4.1.3液態菌種接種後出菇情形…………………………………………….54
4.2多醣含量測定……….………………………………………………………..55
4.2.1搖瓶培養多醣含量測定…………………………………………………56
4.2.2 5L醱酵槽醱酵液多醣含量測定………………………………………..56
4.2.3 乾燥子實體多醣含量測定……………………………………………..59
4.3抗氧化性質分析……………………………………………………………..59
4.3.1捕捉DPPH能力之測定……………………………………………........59
4.3.2還原力之測定……………………………………………………………67
4.3.3螯合亞鐵離子能力之測定……………………………………………...71
4.3.4清除超氧陰離子能力之測定………………………………………….74
4.4腫瘤細胞測試………………………………………………………………..74
4.4.1鴻喜菇子實體及菌絲體之甲醇萃取物對抑制人類肺癌細胞(A549 cells)之影響…………………………………………………………………76
4.4.2鴻喜菇子實體及菌絲體之乙醇萃取物對抑制人類肺癌細胞(A549 cells)之影響………………………………………………………………........76
4.4.3鴻喜菇子實體及菌絲體之熱水萃取物對抑制人類肺癌細胞(A549 cells)之影響……………………………………………………………………80
4.4.4鴻喜菇醱酵液對抑制人類肺癌細胞(A549 cells)之影響………….…..80
4.4.5鴻喜菇子實體及菌絲體之甲醇萃取物對抑制人類乳癌細胞(MCF7 cells)之影響…………………..…………………………………..…….84
4.4.6鴻喜菇子實體及菌絲體之乙醇萃取物對抑制人類乳癌細胞(MCF7 cells)之影響………………………………………………………………84
4.4.7鴻喜菇子實體及菌絲體之熱水萃取物對抑制人類乳癌細胞(MCF7 cells)之影響………………………………………………………………88
4.4.8鴻喜菇醱酵液對抑制人類乳癌細胞(MCF7 cells)之影響…………..88
4.4.9鴻喜菇子實體及菌絲體之甲醇萃取物對抑制人類肝癌細胞(Hep3B cells)之影響…………………………………..……………………….…92
4.4.10鴻喜菇子體及菌絲體之乙醇萃取物對抑制人類肝癌細胞(Hep3B cells)之影響……………………………………………………………………96
4.4.11鴻喜菇子實體及菌絲體之熱水萃取物對抑制人類肝癌細胞(Hep3B cells)之影響……………………………………………………….……..96
4.4.12鴻喜菇醱酵液對抑制人類肝癌細胞(Hep3B cells)之影響…………...96
第五章 結論…………………………………………………………….……….101
參考文獻…………………………………………………………………………104
附錄…
1. Mijazaki, T. and Nishijima, M.. 1981. Studies on fungal polysaccharides. XXVII. Structural examination of a water-soluble, antitumor polysaccharide of Ganoderma lucidum. Chem. Pharm. Bull. 29(12): 3611-3616.
2. Ukai, S.. 1982. Structure of a new galactomannan from the ascocarps of Cordyceps cicadae Shing. Carbohyde. Res. 101: 109.
3. 水野卓、川合正允。賴慶亮譯。1997。菇類的化學、生化學。國立編譯館出版。
4. 王伯徹。2000。食藥用菇保健食品之開發。食品工業。32(5): 18-27。
5. 王伯徹。2000。具開發潛力食藥用菇介紹。食品工業。32(5): 1-17。
6. Mizuno, T. 1995. Bioactive biomolecules of mushrooms: Food function and medicinal effect of mushroom fungi. Food Reviews International 11: 7-21.
7. Jong, S. C. and Birmingham, J. M. 1993. Medicinal therapeutic value of the Shiitake mushroom. Adv Appl Microbiol. 39: 153-184.
8. Wasser, S. P. and Weis, A. L. 1999. Therapeutic effects of substances occurring in higher Basidiomycetes mushroom: a modern perspective. Crit. Rev Immunol 19(1): 65-96.
9. 陳健祺。2000。食用菇類在醫藥上的應用。食品工業。32(5): 54-69。
10. Morigiwa, A., Kitabatake, K., Fujimoto, Y. and Ikekawa, N.. 1986. Angiotensin converting enzyme-inhibitory triterpenes from Ganoderma lucidum. Chem. Pharm. Bull. 34: 3025-3028.
11. Nakai, R., Masui, H., Horio, H. and Ohtsuru, M. 1999. Effect of maitake (Grifola frondosa) water extract on inhibition of adipocyte conversion of C3H10T1/2B2C1 cells. Journal of Nutritional Science & Vitaminology 45:385-389.
12. Kariya, K., Nakamura, K., Nomoto, K., Matama, S. and Saigenji, K.. 1992. Mimicking of superoxide dismutase activity by protein-bound polysaccharide of Coriolus versicolor QUEL, and oxidative stress relief for cancer patients. Molecular Biotherapy 4:40-46.
13. Su, C. H., Sun, C. S., Juan, S. W., Hu, C. H., Ke, W. T. and Sheu, M. T. 1997. Fungal mycelia as the source of chitin and polysaccharides and their application as skin substitutes. Biomaterials 18:1169-1174.
14. Kiho, T., Hui, J., Yamane, A. and Ukai, S.. 1993. Polysaccharides in fungi. XXXII. Hypoglycemic activity and chemical properties of a polysaccharide from the cultural mycelium of Cordyceps sinensis. Biological & Pharmaceutical Bulletin 16:1291-1293.
15. Hikino, H., Konno, C., Mirin, Y. and Hayashi, T.. 1985. Isolation and hypoglycemic activity of ganoderans A and B, glycans of Ganoderma lucidum fruit bodies. Planta Medica. 4:339-340.
16. Park, E. J., Ko, G., Kim, J. and Sohn, D. H.. 1997. Antifibrotic effects of a polysaccharide extracted from Ganoderma lucidum, glycyrrhizin, and pentoxifylline in rats with cirrhoss induced by biliary obstruction. Biological & Pharmaceutical Bulletin 20:417-420.
17. Yamamoto, Y., Shirono, H., Kono, K. and Ohashi, Y.. 1997. Immunopotentiating activity of the water-soluble lignin rich fraction prepared from LEM-the extract of the solide culture medium of Lentinus edodes mycelia. Bioscience Biotechnology & Biochemistry 61:1909-1912.
18. 孫億誠。2000。鴻喜菇採收後品質劣變與保鮮方法之研究。國立中興大學園藝所碩士論文。
19. Ikekawa, T., Saitoh, H., Feng, W., Zhang H., Li, L. and Matsuzawa, T.. 1992. Antitumor activity of hypsizigus marmoreus. I. Antitumor activity of extracts and polysaccharides. Chem Pharm Bull (Tokyo) 40(7): 1954-1957.
20. Tsuchida, K., Aoyagi, Y., Odani, S., Mita, T. and Isemura, M.. 1995. Isolation of a novel collagen-binding protein from the mushroom, hypsiaigus marmoreus, which inhibits the lewis lung carcinoma cell adhesion to type iv collagen. J Biol Chem 270(4): 1481-1484.
21. Shoji, Y., Isemura, M., Muto, H., Isemura, S. and Aoyagi, Y.. 2000. Isolation of a 41-kda protein with cell adhesion activity for animal tumor cells from the mushroom hypsizigus marmoreus by affinity chromatography with type iv collagen immobilized on agarose. Biosci Biotechnol Biochem 64(4): 755-780.
22. Lam, S. K. and Ng, T. B.. 2001. Hypsin, a novel thermostable ribosome-inactivating protein with antifungal and antiproliferative activities from fruiting bodies of the edible mushroom hypsizigus marmoreus. Biochem Biophys Res Commun 285(4): 1071-1075.
23. 歐馨婷。2001。多種蔬菜抑制人類白血病細胞(U937)增殖與誘導其分化之探討。國立台灣大學食品科技研究所碩士論文。
24. 徐麗嵐。2002。以柳松菇與鴻喜菇誘導人類白血病細胞(U937)分化及對Balb/c鼠皮下移植CT26腫瘤之抑制效果。國立台灣大學食品科技研究所碩士論文。
25. Song, C. H., Cho, K. Y. and Nair, N. G.. 1987. Asynthetic medium for the production of submerged cultures of lentinus edodes. Mycologia. 79(6): 866-876.
26. 張淑芬。2001。食藥用菇類搖瓶液體培養條件之探討。食品工業。33(7): 39-46。
27. 王培銘。2002。食藥用菇液態培養製程之開發。食品工業。34(5): 31-35。
28. 陳惠英、顏國欽。1998。自由基、抗氧化防禦與人體健康。Nutritional Sciences Journal, 23(1): 105-121.
29. Green, M. J. and Hill, H. A. O..1984. Chemistry of dioxygen. Methods Enzymol. 105: 3-22.
30. Cadenas, E.. 1995. Mechanisms of oxygen activation and reactive oxygen species detoxification. In Oxidative Stress and Antioxidant Defenses in Biology. Chapman& Hall, New York, USA. 1-61.
31. Halliwell, B., Murcia, M. A., Chirico, S. and Aruoma, O. I.. 1995. Free radicals and antioxidants in food and in vivo: what they do and how they work. Crit. Rev. Food Sci. Nutr. 35: 7-20.
32. Imlay, J. A. and Fridovich, I.. 1991. Assay of metabolic superoxide production in Escherichia coli. J. Biol. Chem. 266: 6957-6965.
33. Halliwel,l B.. 1993. The role of oxygen radicals in human disease, with particular reference to the vascular system. Haemostasis. 23: 118-126.
34. Ames, B. N., Shigenaga, M. K. and Hagen, T. M.. 1993. Oxidants, antioxidants and the degenerative diseases of aging. Proc. Natl. Acad. Sci. USA. 90: 7915-7922.
35. Church, D. F. and Pryor, W. A.. 1985. Free radical chemistry of cigarette smoke and its toxicological implications. Environ. Health Perspect. 64:111-126.
36. Sandy, M. S., Moldeus, P., Ross, D. and Smith, M. T.. 1986. Role of redox cycling and lipid peroxidation in bipyridyl herbicide cytotoxicity. Studies with a compromised isolated hepatocyte model system. Biochem. Pharmacol. 35:3095-3101.
37. Poyer, J. L., McCay, P. B., Lai, E. K., Ganzen, E. G. and Davis, E.R.. 1980. Confirmation of assignment of the trichloromethyl radical spin adduct detected by spin trapping during 13C-carbon tetrachloride metabolism in vitro and in vivo. Biochem. Biophys. Res. Commun. 94:1154-1160.
38. Cavalieri, E. L. and Rogan, E. G.. 1995. Central role of radical cations in metabolic activation of polycyclic aromatic hydrocarbons. Xenobiotica. 25:677-688.
39. Hazlewood, C. and Davies, M. J.. 1995. Damage to DNA and RNA by tumour promoterderived alkoxyl radicals: an EPR spin trapping study. Biochem. Soc. Trans. 23:259S.
40. Frankel, E. N.. 1991. Recent advances in lipid oxidation. J. Sci. Food Agric. 54:495-511.
41. Shahidi, F. and Wanasundara, P. K. J. P. D..1992. Phenolic antioxidants. Crit. Rev. Food Sci. Nutr. 32:67-103.
42. Ichinose, T., Miller, M. G. and Shibamoto, T.. 1994. Determination of free malonaldehyde formed in liver microsomes upon CCl4 oxidation. J. Appl. Toxicol. 14:453-455.
43. Stadtman, E. R. and Oliver, C. N.. 1991. Metal-catalyzed oxidation of proteins. Physiological consequences. J. Biol. Chem. 266: 2005-2008.
44. Neuzil, J., Gebicki, J. M. and Stocker, R.. 1993. Radical-induced chain oxidation of protein and its inhibition by chain-breaking antioxidants. Biochem. J. 293:601-606.
45. Saul R. L. and Ames, B. N.. 1986. Background levels of DNA damage in the population. Basic. Life Sci. 38: 529-535.
46. Branen, A. L. 1975. Toxicology and biochemistry of butylated hydroxyl anisole and butylated hydroxytoluene. J. Am. Oil Chem. Soc. 52: 59-63.
47. Imada, K., Fukushima, S., Shivai, T., Ohtani, M., Nakanishi, K. and Ito, N.. 1983. Promoting activities of butylated hydroxyanisole and butylated hydroxytoluene on 2-stage urinary bladder carcinogenesis and inhibition of γ-glutamyl transpeptidase-positive foci development in the liver of rats. Carcinogenesis 4: 885-889.
48. Ito, N., Fukushima, S. and Tsuda, H.. 1985. Carcinogenicity and modification of the carcinogenic response by BHA, BHT and other antioxidants. CRC Crit. Rev. Toxicol. 15: 109-150.
49. Dziezak, J. D.. 1986. Preservatives: antioxidant. Food Technol. 40: 94-102.
50. Giese, J.. 1996. Antioxidants: Tool for preventing lipid oxidation. Food Tech. November. 73-82.
51. Namiki, M.. 1990. Antioxidants antimutages in food. Cric. Rev. Food Sci. Nutr. 29(4): 273-300.
52. 拱玉郎。1997。天然抗氧化劑發展近況。食品工業29(3): 29-37。
53. 劉姍姍。1996。肉豆蔻揮發性及抗氧化成分之研究。東海大學食品科學研究所碩士論文。
54. 劉伯康。1997。數種傳統食用植物抗佯化性之研究。國立中興大學食品科學系碩士論文。
55. 王子慶。2000。葡萄子與皮抗氧化性之研究。屏東科技大學食品科學系碩士論文。
56. 郭靜娟。2001。薏苡籽實之抗氧化成分及其抑制自由基傷害之研究。國立台灣大學食品科技研究所博士論文。
57. 羅珮文。2001。台灣數種特有水果抗氧化活性及清除自由基能力之評估。輔仁大學食品營養系碩士論文。
58. Yen, G. C., Wu, S. C. and Duh, P. D.. 1996. Extraction and Identification of antioxidant components from the leaves of Mulberry (Morus albal). J. Agric. Food Chem. 44: 1687-1690.
59. Milovanoric, M., Jovanovic, P. J., Radovic, B. V. and Vrbaski, Z.. 1996. Antioxidant effects of flavonoids of Anthriscus sylvestris in lard. J. Am. Oil Chem. Soc. 73: 773-776.
60. Richheimer, S. L., Bernart, M. W., King, G. A., Kent, M. C. and Bailey D. T.. 1996. Antioxidant activity of lipid-soluble phenolic diterpenes from rosemary. J. Am. Oil Chem. Soc. 73: 507-514.
61. Wanasundara, P. K. J. P. D., Shahidi, F. and Shukla, V. K. S. S.. 1997. Endogenous antioxidants from oilseeds and edible oils. Food Rev. Int. 13: 225-292.
62. Angelo, A. J. S.. 1996. Lipid oxidation in foods. Crit. Rev. Food Sci. Nutr. 36(3): 175-224.
63. Torel, J., Cillard, J. and Cillard, P.. 1986. Antioxidant activity of flavonoides and reactivity with peroxy radicals. Phytochem. 25(2): 383-385.
64. Larson, R. A. 1988. The antioxidants of higher plants. Phytochem. 27: 969-978.
65. Burton, G. W.. 1994. Vitamin E: molecular and biological function. Proc Nutr. Soc. 53: 251-262.
66. Rice-Evans, C. A., Miller, N. J. and Paganga, G.. 1996. Structure antioxidant activity relationships of flavonoid and phenolic acids. Free Radical Biol. Med. 20: 933-956.
67. Liebler, D.C.. 1993. antioxidant reactions of carotenoids. Ann. New York. Acad. of Sci. 691: 20-31.
68. Stadler, R. H. and Fay, L. B.. 1995. Antioxidative reactions of caffeine: Formation of 8-oxocaffeine (1,3,7-trimethyiuric acid) in coffee subjected to oxidative stress. J. Agric. Food Chem. 43: 1332-1338.
69. Halliwell, B.. 1994. Free radicals and antioxidants: A personal view. Nutr. Rev. 52(8): 253-265.
70. Cuvelier, M. E., Richard, H. and Berset, C.. 1992. Comparison of the antioxidative activity of some acid-phenols: Structure-activity relationship. Biosci. Biotech. Biochem. 56(2): 324-325.
71. Chen, Q., Shi, H. and Ho, C. T.. 1992. Effects of rosemary extracts and major constituents on lipid oxidation and soybean lipoxygenase activity. J. Am. Oil Chem. Soc. 69(10): 999-1002.
72. Wada, S. and Fang, X.. 1992. The synergistic antioxidant effect of rosemary extract and alpha-tocopherol on sardine oil model system and crushed fish meat. J. Food Proc. Preserv. 16: 263-274.
73. Osawa, T., Sugiyama, Y., Inayoshi, M. and Kawakishi, S.. 1995. Antioxidative activity of tetraphydrocurcuminoids. Biosci. Biotech. Biochem. 59(9): 1609-1612.
74. Cheung, L. M., Cheung, P. C. K. and Ooi, V. E. C. 2003. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chemistry. 81: 249-255.
75. Song, Y. S., Kim, S. H., Sa, J. H., Jin, C., Lim, C. J.and Park, E. H.. 2003. Anti-angiogenic, antioxidant and xanthine oxidase inhibition activities of the mushroom Phellinus linteus. Journal of Ethnopharmacology. 88: 113-116.
76. Yang, J. H., Lin, H. C. and Mau, J. L.. 2002. Antioxidant Properties of several mushrooms. Food Chemistry. 77: 229-235.
77. Mau, J. L., Lin, H. C.and Song, S. F.. 2002. Antioxidant properties of several specialty mushrooms. Food Reaserch International. 35: 519-526.
78. Shyu, Y. S.and Hwang, L. S.. 2002. Antioxidative activity of the crude extract of lignan glycosides from unroasted Burma black sesame meal. Food Res. Inter. 35: 357-365
79. Duh, P. D. and Yen, G. C.. 1997. Antioxidative activity of three herbal water extracts. Food chem. 60(4): 639-645.
80. Amro, B., Aburjai, T. and Al-Khalil, S.. 2002. Antioxidative and radical scavenging effects of olive cake extract. Fitoterapia 73: 456-461.
81. Apáti,P., Szentmihályi, K., Kristó, S. T., Papp, I., Vinkler, P., Szoke, É. and Kéry. Á., 2003. Herbal remedies of Solidago-correlation of phytochemical characteristics and antioxidative properties. Jour. Pharm. and Bio. Analysis. 32: 1045-1053.
82. Dummermuth, A. L., Karsten, U., Fish, K. M., König, G. M. and Wiencke, C.. 2003. Responses of marine macroalgae to hydrogen-peroxide stress. Jour. Exper. Marine Bio. Eco. 289: 103-121.
83. Zainol, M. K., Abd-Hamid, A., Yusof, S. and Muse, R.. 2003. Antioxidative activity and total phenolic compounds of leaf, root and petiole of four accessions of Centella asiatica (L.) Urban. Food Chemistry. 81: 575-581.
84. 黃鈴娟。2000。樟芝與姬松茸之抗氧化性質及其多醣組成分析。國立中興大學食品科學系碩論文。
85. 林秀卿。1999。食藥用菇類之呈味品質和抗氧化性質之評估。國文中興大學食品科學系碩士論文。
86. 邱世浩。2001。培養基之選擇與製備。保健用菇類菌種製作研習班講義。食品工業發展研究所編印。
87. 陳秀男。2003。靈芝多醣體最佳培養條件及對小白鼠之免疫反應研究。國立臺灣大學動物學研究所碩士論文。
88. Liu, F., Ooi, V. E. C. and Chang, S. T.. 1997. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci. 60(10): 763-771.
89. Liu, F. and Ng, T. B.. 2000. Antioxidative and free radical scavenging activities of selected medicinal herbs. Life Sciences. 66(8): 725-735.
90. 張紹欣。2003。天然食材的樟芝發酵酵產物對抑制腫瘤細胞之評估。南台科技大學化學工程研究所生物技術組碩士論文。
91. 蔡淑瑤。2002。靈芝與柳松菇之抗氧化性質和其對腫瘤細胞之毒性及柳松菇之抗致突變性質。國立中興大學食品科學系碩士論文。
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1. 5. 王伯徹。2000。具開發潛力食藥用菇介紹。食品工業。32(5): 1-17。
2. 4. 王伯徹。2000。食藥用菇保健食品之開發。食品工業。32(5): 18-27。
3. 9. 陳健祺。2000。食用菇類在醫藥上的應用。食品工業。32(5): 54-69。
4. 26. 張淑芬。2001。食藥用菇類搖瓶液體培養條件之探討。食品工業。33(7): 39-46。
5. 27. 王培銘。2002。食藥用菇液態培養製程之開發。食品工業。34(5): 31-35。
6. 52. 拱玉郎。1997。天然抗氧化劑發展近況。食品工業29(3): 29-37。
7. (2)朱志忠,林俊一,1994,以實驗計劃法探討製糖濾泥吸收二氧化硫之最適操作條件,技術學刊,第九卷,第二期,pp. 181 - 184。
8. (3)吳全耀、吳黃素月、趙熹、鄭昌家,1994,田口式品質工程在食品加工上的應用-文蛤軟糖的製造,中國水產,493:29 - 40。
9. (4)吳明昌、吳秋霖、林崑狄、吳柏志,2002,以田口方式探討茶焗蛋之製程最適條件,國立屏東科技大學學報,第十一卷,第四期,pp. 239-246。
10. (9)高馥君,1992,反應曲面在食品開發上的應用,食品工業,24(3):32 - 41。
11. (11)許玉芳、林文雄,1998,品質工程之田口實驗設計,建國學報,17:427 - 443。
12. (13)陳和賢、王志源,2001,灰色田口法應用於蛋黃酥油酥皮生產最佳之研究,技術學刊,16(4):549 - 556。
13. (19)潘浙湳,1994,沙寧、田口與傳統實驗設計方法之比較分析,品質管制月刊,30(10)。
 
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