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研究生:洪詠達
研究生(外文):Yang-Ta Hung
論文名稱:表面增強拉曼散射於單一細胞監測技術開發
論文名稱(外文):Surface Enhanced Raman Scattering for single living cell Monitoring
指導教授:江惠華江惠華引用關係房同經房同經引用關係
指導教授(外文):Huihua Kenny ChiangTung-Jing Fang
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:生醫光電工程研究所
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:中文
論文頁數:92
中文關鍵詞:拉曼散射表面增強拉曼散射奈米金粒子細胞表面增強拉曼影像
外文關鍵詞:Raman scatteringSurface Enhanced Raman ScatteringSERSGold nanoparticleCellSurface Enhanced Raman ImageSERI
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  • 被引用被引用:8
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中 文 摘 要
拉曼光譜特性能夠解析分子結構性質,因此透過拉曼顯微光譜儀,能夠提供生物細胞影像多種維度的資訊。但微弱拉曼散射訊號阻礙拉曼光譜在活細胞研究發展,於是結合奈米粒子與表面增強拉曼散射應用在細胞拉曼光譜成為一種重要研究課題。
本研究中,透過化學氧化還原法製備奈米金粒子,藉由調控還原劑-三鈉檸檬酸鹽使用量,製備出不同粒徑的奈米金粒子,由不同粒徑的奈米金粒子中,找尋最佳表面增強拉曼散射效應(增強107到108)。
本研究使用人類牙周韌帶細胞與中國倉鼠卵巢細胞做為測試對象,利用超音波將奈米金粒子導引進入細胞內,因此能夠以積分一秒時間攫取細胞拉曼光譜,實現拉曼光譜即時並且動態監控細胞內物質變化情況的特性。
藉由表面增強拉曼散射,將能夠以極短時間內獲得細胞拉曼光譜。因此,亦將能夠在短時間內利用Mapping的方法建構出細胞表面增強拉曼影像,以提供未來應用表面增強拉曼影像對活細胞做更深入的研究。
Abstract
Raman spectrum characteristics can analyze molecular structure characteristics. Therefore,Raman microspectroscopy can provide the biological cell image in many dimensions of the information. But weak Raman scattering light hinder the Raman spectrum in the research development of living cell. Thereupon, the application of the integration of metal nanopaeticle and Surface-Enhanced Raman Scattering to Raman spectrum of the cell has become an important research topic.
In this research, gold nanoparticle is made by the method of oxidation-reduction. The different sizes of gold nanoparticle is made by the regulative Reductant - three sodiumcitrate. From the different size of gold nanoparticle, the optimum enhanced order of Surface-Enhanced Raman Scattering effect is found to 107 to 108 .
This research used the Human Periodontal Ligament cell and the Chinese hamster ovary cell as the test samples. Gold nanoparticle is led into the cell by Ultrasound. Therefore, Raman spectrum of the cell can be acquired by integral time of one second. It realizes the Raman spectrum real-time. Besides, it monitors dynamically the substance change of the cell.
This research used the Human Periodontal Ligament cell and the Chinese hamster ovary cell as the test samples. Gold nanoparticle is led into the cell by Ultrasound. Therefore, Raman spectrum of the cell can be acquired by integral time of one second. It realizes the Raman spectrum real-time. Besides, it monitors dynamically the substance change of the cell.
參考文獻
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