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研究生(外文):Shao Heng Liou
論文名稱(外文):Studies of magnetism effects on proliferation and reactive oxygen species in liver cancer cells
指導教授:張 寅
指導教授(外文):Yin Chang
中文關鍵詞:正/負磁極Hep G2Hep 3B細胞程式凋亡ROS
外文關鍵詞:positive/negative magnet poleHep G2Hep 3BapoptosisROS
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本研究針對磁極培育人類肝癌細胞株 Hep G2 和 Hep 3B 細胞增殖及 ROS 之影響進行探討。實驗以不加磁極(控制組)及外加磁極進行 Hep G2 和 Hep 3B 之培養。本實驗使用1000、2000、3000及6000 gauss之磁極。在37℃、5% CO2環境下培養, 48 和 96 小時後,收集細胞進行增殖率、DNA 及 ROS 分析。由細胞增殖率結果可知,以正磁極培養 Hep G2 及 Hep 3B 細胞數有高於控制組之趨勢,而以負磁極培養 Hep G2 及 Hep 3B 細胞則有低於控制組趨勢,特別在 Hep 3B/6000gauss負磁極/96hr、Hep G2/6000gauss正磁極/48,96hr有顯著差異(p<0.05);細胞程式凋亡現象觀察之結果,在 Hep G2 及 Hep 3B 與控制組細胞週期並無差異性。另外,由 ROS 測量結果得知,在 Hep G2 細胞在 3000 gauss 正、負磁極培養48及96小時後,以正磁極培養細胞之 ROS 量高於控制組,控制組之 ROS 又高於負磁極培養細胞之 ROS (p<0.05),而 6000 gauss正、負磁極培養48及96小時後,ROS 量均低控制組(p<0.05),至於 ROS 在 Hep 3B 細胞培養96小時後即可呈現與 Hep G2 細胞相同結果。由本研究結果及推論:(1)在3000 gauss 磁場強度下影響的是單一電子自旋態改變,而6000 gauss 磁場強度已足以影響細胞色素高自旋狀態與低自旋狀態;(2) 3000 gauss 強度之負磁極對於正常細胞培養48小時已具有抗氧化效果,而針對 Hep 3B 細胞卻在培養96小時可觀察出有抗氧化效果;(3) 對 Hep G2 cells 而言,以 3000 gauss 正磁極培養48小時及96小時皆會造成 ROS 較控制組增加的趨勢,而 Hep3B cells 以 3000 gauss 正磁極培養96小時亦會造成 ROS 有此現象,因此推論 3000 gauss 正磁極培養時間拉長情況下,可能會有細胞凋亡現象產生。
The purpose of this study is to investigate whether magnet pole can induce the apoptosis in human hepatoma HepG2 and Hep3B cells or not. The magnet pole we used were 1000、2000、3000 and 6000 gauss. The Cells were incubated in the mediums with or without magnet pole at 37℃ in a 5% CO2 humidified atmosphere. Then, the cells were collected and analyzed after incubation. It was found that the proliferation of the cell was significantly decreased in Hep 3B cells which were with the negative pole of 6000 gauss magnet after 96 hours incubation (p<0.05). The proliferation of the cell in Hep G2 which were incubated with the positive pole of 6000 gauss magnet after 48 and 96 hours were also observed(p<0.05). But the cell apoptosis on Hep 3B and Hep G2 were no observed. About the quantity of ROS experimentation, the Hep 3B cells with the 3000 gauss magnet after 48 hours and 96 hours incubation , the positive pole quantity of ROS is higher than control and control higher than negative pole(p<0.05), with the 6000 gauss magnet after 48 hours and 96 hours incubation , the positive and negative pole quantity of ROS is lower than control(p<0.05), but Hep G2 cells only observed on same situation after 96 hours incubation. According to the results we suppose that, (1) 3000 gauss magnetism can change electron spin ; 6000 gauss magnetism can change the cytochrome from high(low) spin state to low(high) spin state, (2) 3000 gauss negative pole were also observed antioxidative, (3) maybe 3000 gauss positive pole can induced Hep 3B dells and Hep G2 cells apoptosis were incubated time longer than 96 hr.
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